Restriction map for
pF1KE1019
(Circular) (Six-base) MAPSORT of: pF1KE1019.seq Check: 4006 from: 1 to: 5102
pF1KE1019 5102 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AatII G_ACGT'C
Cuts at: 620 620
Size: 5102
AccI GT'mk_AC
Cuts at: 2140 2140
Size: 5102
Acc65I G'GTAC_C
Cuts at: 922 2118 922
Size: 1196 3906
AclI AA'CG_TT
Cuts at: 4942 4942
Size: 5102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 1489 1783 1916 2927 3359 3712 4374 1336
Size: 153 294 133 1011 432 353 662 2064
Fragments arranged by size:
2064 1011 662 432 353 294 153 133
AfeI AGC'GCT
Cuts at: 1091 1091
Size: 5102
AflIII A'CryG_T
Cuts at: 2454 4254 4535 2454
Size: 1800 281 3021
Fragments arranged by size:
3021 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1347 2407 3845 1347
Size: 1060 1438 2604
Fragments arranged by size:
2604 1438 1060
ApaLI G'TGCA_C
Cuts at: 3940 3940
Size: 5102
ApoI r'AATT_y
Cuts at: 1483 1609 2106 4455 4711 1483
Size: 126 497 2349 256 1874
Fragments arranged by size:
2349 1874 497 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 5102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
AvaI C'yCGr_G
Cuts at: 216 2122 216
Size: 1906 3196
BamHI G'GATC_C
Cuts at: 1202 2127 1202
Size: 925 4177
BanI G'GyrC_C
Cuts at: 922 2118 2532 2780 2815 922
Size: 1196 414 248 35 3209
Fragments arranged by size:
3209 1196 414 248 35
BanII G_rGCy'C
Cuts at: 847 2116 3146 847
Size: 1269 1030 2803
Fragments arranged by size:
2803 1269 1030
BbeI G_GCGC'C
Cuts at: 2536 2784 2536
Size: 248 4854
BbsI GAAGACnn'nnnn_
Cuts at: 341 341
Size: 5102
BciVI GTATCCnnnnn_n'
Cuts at: 3000 4056 5102 3000
Size: 1056 1046 3000
Fragments arranged by size:
3000 1056 1046
BclI T'GATC_A
Cuts at: 2622 2622
Size: 5102
BfrBI ATG'CAT
Cuts at: 1579 1579
Size: 5102
BglI GCCn_nnn'nGGC
Cuts at: 1378 1378
Size: 5102
BglII A'GATC_T
Cuts at: 2617 4654 2617
Size: 2037 3065
BlpI GC'TnA_GC
Cuts at: 2214 2214
Size: 5102
Bme1580I G_kGCm'C
Cuts at: 2727 2820 3944 2727
Size: 93 1124 3885
Fragments arranged by size:
3885 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2314 2395 2730 4874 2314
Size: 81 335 2144 2542
Fragments arranged by size:
2542 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 588 3469 588
Size: 2881 2221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 509 647 823 1229 105
Size: 404 138 176 406 3978
Fragments arranged by size:
3978 406 404 176 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 1948 2278 3157 3871 4169 603
Size: 1345 330 879 714 298 1536
Fragments arranged by size:
1536 1345 879 714 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 576 4806 576
Size: 4230 872
BsaAI yAC'GTr
Cuts at: 2455 2455
Size: 5102
BsaBI GATnn'nnATC
Cuts at: 6 2048 2616 4653 6
Size: 2042 568 2037 455
Fragments arranged by size:
2042 2037 568 455
BsaHI Gr'CG_yC
Cuts at: 617 775 2533 2781 3483 4979 617
Size: 158 1758 248 702 1496 740
Fragments arranged by size:
1758 1496 740 702 248 158
BsaWI w'CCGG_w
Cuts at: 2291 2812 3490 3901 4048 4322 2291
Size: 521 678 411 147 274 3071
Fragments arranged by size:
3071 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 784
Size: 5102
BseYI C'CCAG_C
Cuts at: 157 1340 1624 2527 3285 3950 4358 157
Size: 1183 284 903 758 665 408 901
Fragments arranged by size:
1183 903 901 758 665 408 284
BsiEI CG_ry'CG
Cuts at: 104 2644 2690 3920 104
Size: 2540 46 1230 1286
Fragments arranged by size:
2540 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 847 2116 2894 3084 3944 847
Size: 1269 778 190 860 2005
Fragments arranged by size:
2005 1269 860 778 190
BsmI GAATG_Cn'
Cuts at: 1130 1130
Size: 5102
BsmBI CGTCTCn'nnnn_
Cuts at: 559 766 559
Size: 207 4895
Bsp1286I G_dGCh'C
Cuts at: 847 2116 2727 2820 2894 3084 3146 3944 847
Size: 1269 611 93 74 190 62 798 2005
Fragments arranged by size:
2005 1269 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1532 3534 1532
Size: 2002 3100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2152 2366 2668 3049 2152
Size: 214 302 381 4205
Fragments arranged by size:
4205 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3394 3448 4932 5097 50
Size: 3344 54 1484 165 55
Fragments arranged by size:
3344 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 3014 1550
Size: 1464 3638
BsrFI r'CCGG_y
Cuts at: 457 3100 3281 457
Size: 2643 181 2278
Fragments arranged by size:
2643 2278 181
BssSI C'ACGA_G
Cuts at: 3373 4081 3373
Size: 708 4394
BstAPI GCAn_nnn'nTGC
Cuts at: 2479 2479
Size: 5102
BstBI TT'CG_AA
Cuts at: 1867 3463 1867
Size: 1596 3506
BstEII G'GTnAC_C
Cuts at: 937 937
Size: 5102
BstXI CCAn_nnnn'nTGG
Cuts at: 1945 1945
Size: 5102
BstYI r'GATC_y
Cuts at: 1 492 1202 2127 2594 2617 2952 3198 3504
Size: 491 710 925 467 23 335 246 306
Cuts at: 3504 3516 3602 4654 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 925 710 491 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 767 1832 767
Size: 1065 4037
BtgI C'CryG_G
Cuts at: 1877 1877
Size: 5102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 890 3052 3233 3396 890
Size: 2162 181 163 2596
Fragments arranged by size:
2596 2162 181 163
BtsI GCAGTG_nn'
Cuts at: 1345 2345 1345
Size: 1000 4102
ClaI AT'CG_AT
Cuts at: 294 294
Size: 5102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 915 950 915
Size: 35 5067
DraI TTT'AAA
Cuts at: 2101 2101
Size: 5102
DrdI GACnn_nn'nnGTC
Cuts at: 175 2808 4152 175
Size: 2633 1344 1125
Fragments arranged by size:
2633 1344 1125
EaeI y'GGCC_r
Cuts at: 231 2687 2861 3252 3279 4624 231
Size: 2456 174 391 27 1345 709
Fragments arranged by size:
2456 1345 709 391 174 27
EagI C'GGCC_G
Cuts at: 2687 2687
Size: 5102
EarI CTCTTCn'nnn_
Cuts at: 434 478 711 743 859 1163 1327 3125 3335
Size: 44 233 32 116 304 164 1798 210
Cuts at: 3335 434
Size: 2201
Fragments arranged by size:
2201 1798 304 233 210 164 116 44
32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3310 4052 4198 4912 3310
Size: 742 146 714 3500
Fragments arranged by size:
3500 742 714 146
EcoICRI GAG'CTC
Cuts at: 845 2114 845
Size: 1269 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 509 647 823 1229 1336 1489 1783 1916
Size: 404 138 176 406 107 153 294 133
Cuts at: 1916 2927 3359 3712 4374 105
Size: 1011 432 353 662 833
Fragments arranged by size:
1011 833 662 432 406 404 353 294
176 153 138 133 107
EcoO109I rG'GnC_Cy
Cuts at: 1473 2241 1473
Size: 768 4334
EcoRI G'AATT_C
Cuts at: 2106 2106
Size: 5102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 463 3121 463
Size: 2658 2444
FspI TGC'GCA
Cuts at: 2883 2883
Size: 5102
HaeII r_GCGC'y
Cuts at: 1093 2536 2784 4014 4785 1093
Size: 1443 248 1230 771 1410
Fragments arranged by size:
1443 1410 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 715 747 2944 2974 2976 3006 310
Size: 32 373 32 2197 30 2 30 2406
Fragments arranged by size:
2406 2197 373 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2141 2141
Size: 5102
KasI G'GCGC_C
Cuts at: 2532 2780 2532
Size: 248 4854
KpnI G_GTAC'C
Cuts at: 926 2122 926
Size: 1196 3906
MluI A'CGCG_T
Cuts at: 4535 4535
Size: 5102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1420 3861 4045 1420
Size: 2441 184 2477
Fragments arranged by size:
2477 2441 184
MscI TGG'CCA
Cuts at: 233 2863 233
Size: 2630 2472
MslI CAynn'nnrTG
Cuts at: 2035 3091 3218 2035
Size: 1056 127 3919
Fragments arranged by size:
3919 1056 127
MspA1I CmG'CkG
Cuts at: 161 1624 2213 2527 2887 3671 3916 161
Size: 1463 589 314 360 784 245 1347
Fragments arranged by size:
1463 1347 784 589 360 314 245
NaeI GCC'GGC
Cuts at: 3283 3283
Size: 5102
NarI GG'CG_CC
Cuts at: 2533 2781 2533
Size: 248 4854
NcoI C'CATG_G
Cuts at: 1877 1877
Size: 5102
NgoMIV G'CCGG_C
Cuts at: 3281 3281
Size: 5102
NsiI A_TGCA'T
Cuts at: 1581 1581
Size: 5102
NspI r_CATG'y
Cuts at: 2155 2655 4258 2155
Size: 500 1603 2999
Fragments arranged by size:
2999 1603 500
PasI CC'CwG_GG
Cuts at: 129 129
Size: 5102
PciI A'CATG_T
Cuts at: 4254 4254
Size: 5102
PflMI CCAn_nnn'nTGG
Cuts at: 113 135 113
Size: 22 5080
PmeI GTTT'AAAC
Cuts at: 2101 2101
Size: 5102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 5070
PsiI TTA'TAA
Cuts at: 2080 2080
Size: 5102
PspXI vC'TCGA_Gb
Cuts at: 216 216
Size: 5102
PstI C_TGCA'G
Cuts at: 2149 2834 2149
Size: 685 4417
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5102
PvuII CAG'CTG
Cuts at: 1624 2527 2887 1624
Size: 903 360 3839
Fragments arranged by size:
3839 903 360
RsrII CG'GwC_CG
Cuts at: 3297 3297
Size: 5102
SacI G_AGCT'C
Cuts at: 847 2116 847
Size: 1269 3833
SalI G'TCGA_C
Cuts at: 2139 2139
Size: 5102
SapI GCTCTTCn'nnn_
Cuts at: 859 1327 3125 3335 859
Size: 468 1798 210 2626
Fragments arranged by size:
2626 1798 468 210
SbfI CC_TGCA'GG
Cuts at: 2149 2149
Size: 5102
ScaI AGT'ACT
Cuts at: 4619 4619
Size: 5102
SfcI C'TryA_G
Cuts at: 33 1414 2145 2830 3798 3989 33
Size: 1381 731 685 968 191 1146
Fragments arranged by size:
1381 1146 968 731 685 191
SfoI GGC'GCC
Cuts at: 2534 2782 2534
Size: 248 4854
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5102
SmaI CCC'GGG
Cuts at: 2124 2124
Size: 5102
SmlI C'TyrA_G
Cuts at: 216 582 1963 2257 3172 3886 4148 216
Size: 366 1381 294 915 714 262 1170
Fragments arranged by size:
1381 1170 915 714 366 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5102
SphI G_CATG'C
Cuts at: 2155 2655 2155
Size: 500 4602
SspI AAT'ATT
Cuts at: 2004 2004
Size: 5102
StuI AGG'CCT
Cuts at: 1193 1193
Size: 5102
StyI C'CwwG_G
Cuts at: 711 848 1877 2236 2590 711
Size: 137 1029 359 354 3223
Fragments arranged by size:
3223 1029 359 354 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1796 2685 2818 3277 3486 1796
Size: 889 133 459 209 3412
Fragments arranged by size:
3412 889 459 209 133
TatI w'GTAC_w
Cuts at: 236 1810 1917 4617 236
Size: 1574 107 2700 721
Fragments arranged by size:
2700 1574 721 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 427 459 4619 4651 427
Size: 32 4160 32 878
Fragments arranged by size:
4160 878 32 32
Tth111I GACn'n_nGTC
Cuts at: 2899 2899
Size: 5102
XbaI T'CTAG_A
Cuts at: 2133 2133
Size: 5102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 2069 2597 270
Size: 1799 528 2775
Fragments arranged by size:
2775 1799 528
XhoI C'TCGA_G
Cuts at: 216 216
Size: 5102
XmaI C'CCGG_G
Cuts at: 2122 2122
Size: 5102
XmnI GAAnn'nnTTC
Cuts at: 1321 1321
Size: 5102
ZraI GAC'GTC
Cuts at: 618 618
Size: 5102
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BspEI BsrGI BssHII BstZ17I DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PfoI PmlI PpuMI PshAI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI