Restriction map for
pF1KE1020
(Circular) (Six-base) MAPSORT of: pF1KE1020.seq Check: 3848 from: 1 to: 5117
pF1KE1020 5117 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1827 1929 1827
Size: 102 5015
AccI GT'mk_AC
Cuts at: 871 1067 2155 871
Size: 196 1088 3833
Fragments arranged by size:
3833 1088 196
Acc65I G'GTAC_C
Cuts at: 2059 2133 2059
Size: 74 5043
AclI AA'CG_TT
Cuts at: 4957 4957
Size: 5117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 615 1349 1479 1608 1718 2942 3374 3727
Size: 372 734 130 129 110 1224 432 353
Cuts at: 3727 4389 243
Size: 662 971
Fragments arranged by size:
1224 971 734 662 432 372 353 130
129 110
AflIII A'CryG_T
Cuts at: 1070 2469 4269 4550 1070
Size: 1399 1800 281 1637
Fragments arranged by size:
1800 1637 1399 281
AgeI A'CCGG_T
Cuts at: 1376 1376
Size: 5117
AhdI GACnn_n'nnGTC
Cuts at: 1086 1086
Size: 5117
AleI CACnn'nnGTG
Cuts at: 385 385
Size: 5117
AlwNI CAG_nnn'CTG
Cuts at: 207 570 638 2422 3860 207
Size: 363 68 1784 1438 1464
Fragments arranged by size:
1784 1464 1438 363 68
ApaI G_GGCC'C
Cuts at: 615 1261 1813 615
Size: 646 552 3919
Fragments arranged by size:
3919 646 552
ApaLI G'TGCA_C
Cuts at: 3955 3955
Size: 5117
ApoI r'AATT_y
Cuts at: 826 2121 4470 4726 826
Size: 1295 2349 256 1217
Fragments arranged by size:
2349 1295 1217 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5117
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
AvaI C'yCGr_G
Cuts at: 476 605 1417 1439 1475 1806 2137 476
Size: 129 812 22 36 331 331 3456
Fragments arranged by size:
3456 812 331 331 129 36 22
BamHI G'GATC_C
Cuts at: 2142 2142
Size: 5117
BanI G'GyrC_C
Cuts at: 1286 1348 1529 1769 1941 2059 2133 2547 2795
Size: 62 181 240 172 118 74 414 248
Cuts at: 2795 2830 1286
Size: 35 3573
Fragments arranged by size:
3573 414 248 240 181 172 118 74
62 35
BanII G_rGCy'C
Cuts at: 615 647 1129 1171 1261 1482 1691 1813 1926
Size: 32 482 42 90 221 209 122 113
Cuts at: 1926 2011 2111 2131 3161 615
Size: 85 100 20 1030 2571
Fragments arranged by size:
2571 1030 482 221 209 122 113 100
90 85 42 32 20
BbeI G_GCGC'C
Cuts at: 1290 1773 2551 2799 1290
Size: 483 778 248 3608
Fragments arranged by size:
3608 778 483 248
BbvCI CC'TCA_GC
Cuts at: 1019 1234 1423 2077 1019
Size: 215 189 654 4059
Fragments arranged by size:
4059 654 215 189
BciVI GTATCCnnnnn_n'
Cuts at: 3015 4071 5117 3015
Size: 1056 1046 3015
Fragments arranged by size:
3015 1056 1046
BclI T'GATC_A
Cuts at: 2637 2637
Size: 5117
BglI GCCn_nnn'nGGC
Cuts at: 610 1223 1256 1958 1967 610
Size: 613 33 702 9 3760
Fragments arranged by size:
3760 702 613 33 9
BglII A'GATC_T
Cuts at: 2001 2632 4669 2001
Size: 631 2037 2449
Fragments arranged by size:
2449 2037 631
BlpI GC'TnA_GC
Cuts at: 430 2229 430
Size: 1799 3318
Bme1580I G_kGCm'C
Cuts at: 615 845 938 1261 1353 1532 1752 1781 1813
Size: 230 93 323 92 179 220 29 32
Cuts at: 1813 1946 2742 2835 3959 615
Size: 133 796 93 1124 1773
Fragments arranged by size:
1773 1124 796 323 230 220 179 133
93 93 92 32 29
BmgBI CAC'GTC
Cuts at: 779 1071 779
Size: 292 4825
BmrI ACTGGGnnnn_n'
Cuts at: 769 925 1354 1904 2114 2329 2410 2745 4889
Size: 156 429 550 210 215 81 335 2144
Cuts at: 4889 769
Size: 997
Fragments arranged by size:
2144 997 550 429 335 215 210 156
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 1996 3484 631
Size: 1365 1488 2264
Fragments arranged by size:
2264 1488 1365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 841 1177 1590 1867 1940 2071 114
Size: 727 336 413 277 73 131 3160
Fragments arranged by size:
3160 727 413 336 277 131 73
Bpu10I CC'TnA_GC
Cuts at: 737 1019 1234 1423 1506 2077 737
Size: 282 215 189 83 571 3777
Fragments arranged by size:
3777 571 282 215 189 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1669 2293 3172 3886 4184 601
Size: 1068 624 879 714 298 1534
Fragments arranged by size:
1534 1068 879 714 624 298
BsaI GGTCTCn'nnnn_
Cuts at: 1094 4821 1094
Size: 3727 1390
BsaAI yAC'GTr
Cuts at: 908 1747 2470 908
Size: 839 723 3555
Fragments arranged by size:
3555 839 723
BsaBI GATnn'nnATC
Cuts at: 6 2631 4668 6
Size: 2625 2037 455
Fragments arranged by size:
2625 2037 455
BsaHI Gr'CG_yC
Cuts at: 1287 1770 2028 2548 2796 3498 4994 1287
Size: 483 258 520 248 702 1496 1410
Fragments arranged by size:
1496 1410 702 520 483 258 248
BsaWI w'CCGG_w
Cuts at: 1376 2306 2827 3505 3916 4063 4337 1376
Size: 930 521 678 411 147 274 2156
Fragments arranged by size:
2156 930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 901 931 1132 1162 901
Size: 30 201 30 4856
Fragments arranged by size:
4856 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 1004 1529 529
Size: 475 525 4117
Fragments arranged by size:
4117 525 475
BseYI C'CCAG_C
Cuts at: 154 216 322 789 1455 2542 3300 3965 4373
Size: 62 106 467 666 1087 758 665 408
Cuts at: 4373 154
Size: 898
Fragments arranged by size:
1087 898 758 666 665 467 408 106
62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 1799 759
Size: 1040 4077
BsiEI CG_ry'CG
Cuts at: 104 2659 2705 3935 104
Size: 2555 46 1230 1286
Fragments arranged by size:
2555 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 236 419 496 1926 2011 2131 2909 3099 3959
Size: 183 77 1430 85 120 778 190 860
Cuts at: 3959 236
Size: 1394
Fragments arranged by size:
1430 1394 860 778 190 183 120 85
77
BsmI GAATG_Cn'
Cuts at: 468 468
Size: 5117
BsmBI CGTCTCn'nnnn_
Cuts at: 2035 2035
Size: 5117
Bsp1286I G_dGCh'C
Cuts at: 236 419 496 615 647 845 938 1129 1171
Size: 183 77 119 32 198 93 191 42
Cuts at: 1171 1261 1353 1482 1532 1691 1752 1781 1813
Size: 90 92 129 50 159 61 29 32
Cuts at: 1813 1926 1946 2011 2111 2131 2742 2835 2909
Size: 113 20 65 100 20 611 93 74
Cuts at: 2909 3099 3161 3959 236
Size: 190 62 798 1394
Fragments arranged by size:
1394 798 611 198 191 190 183 159
129 119 113 100 93 93 92 90
77 74 65 62 61 50 42 32
32 29 20 20
BspHI T'CATG_A
Cuts at: 3549 3549
Size: 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 883 1827 1929 2167 2381 2683 3064 632
Size: 251 944 102 238 214 302 381 2685
Fragments arranged by size:
2685 944 381 302 251 238 214 102
BsrBI CCG'CTC
Cuts at: 50 137 741 1307 3409 3463 4947 5112 50
Size: 87 604 566 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 604 566 165 87 55 54
BsrDI GCAATG_nn'
Cuts at: 3029 3029
Size: 5117
BsrFI r'CCGG_y
Cuts at: 149 1222 1376 1532 1961 3115 3296 149
Size: 1073 154 156 429 1154 181 1970
Fragments arranged by size:
1970 1154 1073 429 181 156 154
BsrGI T'GTAC_A
Cuts at: 670 670
Size: 5117
BssSI C'ACGA_G
Cuts at: 718 1035 1146 3388 4096 718
Size: 317 111 2242 708 1739
Fragments arranged by size:
2242 1739 708 317 111
BstAPI GCAn_nnn'nTGC
Cuts at: 207 2494 207
Size: 2287 2830
BstBI TT'CG_AA
Cuts at: 3478 3478
Size: 5117
BstEII G'GTnAC_C
Cuts at: 252 1111 252
Size: 859 4258
BstYI r'GATC_y
Cuts at: 1 2001 2142 2609 2632 2967 3213 3519 3531
Size: 2000 141 467 23 335 246 306 12
Cuts at: 3531 3617 4669 1
Size: 86 1052 449
Fragments arranged by size:
2000 1052 467 449 335 306 246 141
86 23 12
BstZ17I GTA'TAC
Cuts at: 872 872
Size: 5117
BtgI C'CryG_G
Cuts at: 920 1076 1181 1282 1294 1573 920
Size: 156 105 101 12 279 4464
Fragments arranged by size:
4464 279 156 105 101 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3067 3248 3411 3067
Size: 181 163 4773
Fragments arranged by size:
4773 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 2360 1613
Size: 747 4370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 993 1028 993
Size: 35 5082
DraI TTT'AAA
Cuts at: 2116 2116
Size: 5117
DraIII CAC_nnn'GTG
Cuts at: 1348 1348
Size: 5117
DrdI GACnn_nn'nnGTC
Cuts at: 2823 4167 2823
Size: 1344 3773
EaeI y'GGCC_r
Cuts at: 373 1390 1852 1968 2040 2702 2876 3267 3294
Size: 1017 462 116 72 662 174 391 27
Cuts at: 3294 4639 373
Size: 1345 851
Fragments arranged by size:
1345 1017 851 662 462 391 174 116
72 27
EagI C'GGCC_G
Cuts at: 2702 2702
Size: 5117
EarI CTCTTCn'nnn_
Cuts at: 279 634 1714 2022 3140 3350 279
Size: 355 1080 308 1118 210 2046
Fragments arranged by size:
2046 1118 1080 355 308 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 169 1702 3325 4067 4213 4927 169
Size: 1533 1623 742 146 714 359
Fragments arranged by size:
1623 1533 742 714 359 146
EcoICRI GAG'CTC
Cuts at: 1924 2009 2129 1924
Size: 85 120 4912
Fragments arranged by size:
4912 120 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 243 615 841 1177 1349 1479 1590 1608
Size: 129 372 226 336 172 130 111 18
Cuts at: 1608 1718 1867 1940 2071 2942 3374 3727 4389
Size: 110 149 73 131 871 432 353 662
Cuts at: 4389 114
Size: 842
Fragments arranged by size:
871 842 662 432 372 353 336 226
172 149 131 130 129 111 110 73
18
EcoO109I rG'GnC_Cy
Cuts at: 165 204 305 611 612 763 1257 1258 1405
Size: 39 101 306 1 151 494 1 147
Cuts at: 1405 1434 1629 1810 1836 1863 2256 165
Size: 29 195 181 26 27 393 3026
Fragments arranged by size:
3026 494 393 306 195 181 151 147
101 39 29 27 26 1 1
EcoRI G'AATT_C
Cuts at: 2121 2121
Size: 5117
EcoRV GAT'ATC
Cuts at: 1889 1889
Size: 5117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 5117
FseI GG_CCGG'CC
Cuts at: 1965 1965
Size: 5117
FspI TGC'GCA
Cuts at: 2898 2898
Size: 5117
HaeII r_GCGC'y
Cuts at: 1290 1773 2551 2799 4029 4800 1290
Size: 483 778 248 1230 771 1607
Fragments arranged by size:
1607 1230 778 771 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1072 1104 1908 1940 1993 2025 2959 2989 2991
Size: 32 804 32 53 32 934 30 2
Cuts at: 2991 3021 1072
Size: 30 3168
Fragments arranged by size:
3168 934 804 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2156 2156
Size: 5117
KasI G'GCGC_C
Cuts at: 1286 1769 2547 2795 1286
Size: 483 778 248 3608
Fragments arranged by size:
3608 778 483 248
KpnI G_GTAC'C
Cuts at: 2063 2137 2063
Size: 74 5043
MluI A'CGCG_T
Cuts at: 4550 4550
Size: 5117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 510 1118 1361 3876 4060 510
Size: 608 243 2515 184 1567
Fragments arranged by size:
2515 1567 608 243 184
MscI TGG'CCA
Cuts at: 1854 2878 1854
Size: 1024 4093
MslI CAynn'nnrTG
Cuts at: 247 385 3106 3233 247
Size: 138 2721 127 2131
Fragments arranged by size:
2721 2131 138 127
MspA1I CmG'CkG
Cuts at: 1085 1360 1370 1563 1724 1850 1994 2228 2542
Size: 275 10 193 161 126 144 234 314
Cuts at: 2542 2902 3686 3931 1085
Size: 360 784 245 2271
Fragments arranged by size:
2271 784 360 314 275 245 234 193
161 144 126 10
NaeI GCC'GGC
Cuts at: 151 1224 1963 3298 151
Size: 1073 739 1335 1970
Fragments arranged by size:
1970 1335 1073 739
NarI GG'CG_CC
Cuts at: 1287 1770 2548 2796 1287
Size: 483 778 248 3608
Fragments arranged by size:
3608 778 483 248
NcoI C'CATG_G
Cuts at: 920 1076 920
Size: 156 4961
NgoMIV G'CCGG_C
Cuts at: 149 1222 1961 3296 149
Size: 1073 739 1335 1970
Fragments arranged by size:
1970 1335 1073 739
NspI r_CATG'y
Cuts at: 2170 2670 4273 2170
Size: 500 1603 3014
Fragments arranged by size:
3014 1603 500
PasI CC'CwG_GG
Cuts at: 1120 1397 1830 1120
Size: 277 433 4407
Fragments arranged by size:
4407 433 277
PciI A'CATG_T
Cuts at: 4269 4269
Size: 5117
PflMI CCAn_nnn'nTGG
Cuts at: 1380 1830 1380
Size: 450 4667
PfoI T'CCnGG_A
Cuts at: 364 1891 364
Size: 1527 3590
PmeI GTTT'AAAC
Cuts at: 2116 2116
Size: 5117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 5085
PpuMI rG'GwC_Cy
Cuts at: 305 763 1405 1434 1629 1836 305
Size: 458 642 29 195 207 3586
Fragments arranged by size:
3586 642 458 207 195 29
PspOMI G'GGCC_C
Cuts at: 611 1257 1809 611
Size: 646 552 3919
Fragments arranged by size:
3919 646 552
PstI C_TGCA'G
Cuts at: 203 880 1166 1680 2164 2849 203
Size: 677 286 514 484 685 2471
Fragments arranged by size:
2471 685 677 514 484 286
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5117
PvuII CAG'CTG
Cuts at: 1360 1370 1850 1994 2542 2902 1360
Size: 10 480 144 548 360 3575
Fragments arranged by size:
3575 548 480 360 144 10
RsrII CG'GwC_CG
Cuts at: 3312 3312
Size: 5117
SacI G_AGCT'C
Cuts at: 1926 2011 2131 1926
Size: 85 120 4912
Fragments arranged by size:
4912 120 85
SalI G'TCGA_C
Cuts at: 2154 2154
Size: 5117
SanDI GG'GwC_CC
Cuts at: 1405 1434 1405
Size: 29 5088
SapI GCTCTTCn'nnn_
Cuts at: 634 2022 3140 3350 634
Size: 1388 1118 210 2401
Fragments arranged by size:
2401 1388 1118 210
SbfI CC_TGCA'GG
Cuts at: 2164 2164
Size: 5117
ScaI AGT'ACT
Cuts at: 4634 4634
Size: 5117
SfcI C'TryA_G
Cuts at: 33 199 876 1162 1676 2160 2845 3813 4004
Size: 166 677 286 514 484 685 968 191
Cuts at: 4004 33
Size: 1146
Fragments arranged by size:
1146 968 685 677 514 484 286 191
166
SfiI GGCCn_nnn'nGGCC
Cuts at: 1256 1967 1256
Size: 711 4406
SfoI GGC'GCC
Cuts at: 1288 1771 2549 2797 1288
Size: 483 778 248 3608
Fragments arranged by size:
3608 778 483 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
SmaI CCC'GGG
Cuts at: 1441 2139 1441
Size: 698 4419
SmlI C'TyrA_G
Cuts at: 616 1648 2272 3187 3901 4163 616
Size: 1032 624 915 714 262 1570
Fragments arranged by size:
1570 1032 915 714 624 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5117
SphI G_CATG'C
Cuts at: 2170 2670 2170
Size: 500 4617
StyI C'CwwG_G
Cuts at: 920 1076 2251 2605 920
Size: 156 1175 354 3432
Fragments arranged by size:
3432 1175 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1336 1389 2700 2833 3292 3501 1336
Size: 53 1311 133 459 209 2952
Fragments arranged by size:
2952 1311 459 209 133 53
TatI w'GTAC_w
Cuts at: 670 4632 670
Size: 3962 1155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1132 1164 4634 4666 1132
Size: 32 3470 32 1583
Fragments arranged by size:
3470 1583 32 32
Tth111I GACn'n_nGTC
Cuts at: 2914 2914
Size: 5117
XbaI T'CTAG_A
Cuts at: 2148 2148
Size: 5117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 816 2612 816
Size: 1796 3321
XmaI C'CCGG_G
Cuts at: 1439 2137 1439
Size: 698 4419
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BspEI BssHII BstXI
Bsu36I ClaI EcoNI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI