Restriction map for
pF1KE1022
(Circular) (Six-base) MAPSORT of: pF1KE1022.seq Check: 3651 from: 1 to: 5303
pF1KE1022 5303 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1165 1388 1165
Size: 223 5080
AccI GT'mk_AC
Cuts at: 2341 2341
Size: 5303
Acc65I G'GTAC_C
Cuts at: 2319 2319
Size: 5303
AclI AA'CG_TT
Cuts at: 5143 5143
Size: 5303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 850 923 1276 1406 1852 1972 2056 3128
Size: 72 73 353 130 446 120 84 1072
Cuts at: 3128 3560 3913 4575 778
Size: 432 353 662 1506
Fragments arranged by size:
1506 1072 662 446 432 353 353 130
120 84 73 72
AfeI AGC'GCT
Cuts at: 1201 2045 1201
Size: 844 4459
AflIII A'CryG_T
Cuts at: 991 2655 4455 4736 991
Size: 1664 1800 281 1558
Fragments arranged by size:
1800 1664 1558 281
AhdI GACnn_n'nnGTC
Cuts at: 2099 2099
Size: 5303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 683 715 1011 1043 1162 1194 683
Size: 32 296 32 119 32 4792
Fragments arranged by size:
4792 296 119 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 828 1190 2608 4046 828
Size: 362 1418 1438 2085
Fragments arranged by size:
2085 1438 1418 362
ApaI G_GGCC'C
Cuts at: 2182 2182
Size: 5303
ApaLI G'TGCA_C
Cuts at: 907 4141 907
Size: 3234 2069
ApoI r'AATT_y
Cuts at: 2307 4656 4912 2307
Size: 2349 256 2698
Fragments arranged by size:
2698 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5303
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
AvaI C'yCGr_G
Cuts at: 1302 2229 2323 1302
Size: 927 94 4282
Fragments arranged by size:
4282 927 94
BamHI G'GATC_C
Cuts at: 1719 2328 1719
Size: 609 4694
BanI G'GyrC_C
Cuts at: 521 1205 1564 2123 2319 2733 2981 3016 521
Size: 684 359 559 196 414 248 35 2808
Fragments arranged by size:
2808 684 559 414 359 248 196 35
BanII G_rGCy'C
Cuts at: 412 2084 2182 2317 3347 412
Size: 1672 98 135 1030 2368
Fragments arranged by size:
2368 1672 1030 135 98
BbeI G_GCGC'C
Cuts at: 2737 2985 2737
Size: 248 5055
BbsI GAAGACnn'nnnn_
Cuts at: 929 1264 1394 1696 929
Size: 335 130 302 4536
Fragments arranged by size:
4536 335 302 130
BbvCI CC'TCA_GC
Cuts at: 320 1134 1139 320
Size: 814 5 4484
Fragments arranged by size:
4484 814 5
BciVI GTATCCnnnnn_n'
Cuts at: 3201 4257 5303 3201
Size: 1056 1046 3201
Fragments arranged by size:
3201 1056 1046
BclI T'GATC_A
Cuts at: 836 2823 836
Size: 1987 3316
BglII A'GATC_T
Cuts at: 2818 4855 2818
Size: 2037 3266
BlpI GC'TnA_GC
Cuts at: 406 2415 406
Size: 2009 3294
Bme1580I G_kGCm'C
Cuts at: 778 911 1051 1567 2182 2928 3021 4145 778
Size: 133 140 516 615 746 93 1124 1936
Fragments arranged by size:
1936 1124 746 615 516 140 133 93
BmrI ACTGGGnnnn_n'
Cuts at: 1076 1755 2515 2596 2931 5075 1076
Size: 679 760 81 335 2144 1304
Fragments arranged by size:
2144 1304 760 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 1146 3670 561
Size: 585 2524 2194
Fragments arranged by size:
2524 2194 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1960 1981 2023 2029 2096 2136 943
Size: 1017 21 42 6 67 40 4110
Fragments arranged by size:
4110 1017 67 42 40 21 6
Bpu10I CC'TnA_GC
Cuts at: 320 1134 1139 320
Size: 814 5 4484
Fragments arranged by size:
4484 814 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2479 3358 4072 4370 1624
Size: 855 879 714 298 2557
Fragments arranged by size:
2557 879 855 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 262 939 5007 262
Size: 677 4068 558
Fragments arranged by size:
4068 677 558
BsaAI yAC'GTr
Cuts at: 719 2656 719
Size: 1937 3366
BsaBI GATnn'nnATC
Cuts at: 6 2817 4854 6
Size: 2811 2037 455
Fragments arranged by size:
2811 2037 455
BsaHI Gr'CG_yC
Cuts at: 566 1663 2734 2982 3684 5180 566
Size: 1097 1071 248 702 1496 689
Fragments arranged by size:
1496 1097 1071 702 689 248
BsaWI w'CCGG_w
Cuts at: 423 2126 2492 3013 3691 4102 4249 4523 423
Size: 1703 366 521 678 411 147 274 1203
Fragments arranged by size:
1703 1203 678 521 411 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 683 713 1162 1192 1954 1984 683
Size: 30 449 30 762 30 4002
Fragments arranged by size:
4002 762 449 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 284 505 508 553 878 1122 1834 1837 1957
Size: 221 3 45 325 244 712 3 120
Cuts at: 1957 1978 1981 284
Size: 21 3 3606
Fragments arranged by size:
3606 712 325 244 221 120 45 21
3 3 3
BseYI C'CCAG_C
Cuts at: 949 1588 1865 2728 3486 4151 4559 949
Size: 639 277 863 758 665 408 1693
Fragments arranged by size:
1693 863 758 665 639 408 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1438 1438
Size: 5303
BsiEI CG_ry'CG
Cuts at: 104 2204 2845 2891 4121 104
Size: 2100 641 46 1230 1286
Fragments arranged by size:
2100 1286 1230 641 46
BsiHKAI G_wGCw'C
Cuts at: 911 1178 2317 3095 3285 4145 911
Size: 267 1139 778 190 860 2069
Fragments arranged by size:
2069 1139 860 778 267 190
BsmI GAATG_Cn'
Cuts at: 1758 1758
Size: 5303
BsmBI CGTCTCn'nnnn_
Cuts at: 139 1417 139
Size: 1278 4025
Bsp1286I G_dGCh'C
Cuts at: 412 778 911 1051 1178 1567 2084 2182 2317
Size: 366 133 140 127 389 517 98 135
Cuts at: 2317 2928 3021 3095 3285 3347 4145 412
Size: 611 93 74 190 62 798 1570
Fragments arranged by size:
1570 798 611 517 389 366 190 140
135 133 127 98 93 74 62
BspEI T'CCGG_A
Cuts at: 423 423
Size: 5303
BspHI T'CATG_A
Cuts at: 1523 3735 1523
Size: 2212 3091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1388 2353 2567 2869 3250 1165
Size: 223 965 214 302 381 3218
Fragments arranged by size:
3218 965 381 302 223 214
BsrBI CCG'CTC
Cuts at: 50 1281 1897 3595 3649 5133 5298 50
Size: 1231 616 1698 54 1484 165 55
Fragments arranged by size:
1698 1484 1231 616 165 55 54
BsrDI GCAATG_nn'
Cuts at: 355 3215 355
Size: 2860 2443
BsrFI r'CCGG_y
Cuts at: 1915 1973 2187 3301 3482 1915
Size: 58 214 1114 181 3736
Fragments arranged by size:
3736 1114 214 181 58
BssSI C'ACGA_G
Cuts at: 751 3574 4282 751
Size: 2823 708 1772
Fragments arranged by size:
2823 1772 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2259 2680 2259
Size: 421 4882
BstBI TT'CG_AA
Cuts at: 1750 3664 1750
Size: 1914 3389
BstXI CCAn_nnnn'nTGG
Cuts at: 555 555
Size: 5303
BstYI r'GATC_y
Cuts at: 1 314 1719 2328 2795 2818 3153 3399 3705
Size: 313 1405 609 467 23 335 246 306
Cuts at: 3705 3717 3803 4855 1
Size: 12 86 1052 449
Fragments arranged by size:
1405 1052 609 467 449 335 313 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 548 869 1284 1658 1724 107
Size: 441 321 415 374 66 3686
Fragments arranged by size:
3686 441 415 374 321 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 472 3253 3434 3597 472
Size: 2781 181 163 2178
Fragments arranged by size:
2781 2178 181 163
BtsI GCAGTG_nn'
Cuts at: 200 829 2546 200
Size: 629 1717 2957
Fragments arranged by size:
2957 1717 629
DraI TTT'AAA
Cuts at: 2302 2302
Size: 5303
DrdI GACnn_nn'nnGTC
Cuts at: 1431 3009 4353 1431
Size: 1578 1344 2381
Fragments arranged by size:
2381 1578 1344
EaeI y'GGCC_r
Cuts at: 606 1350 2072 2201 2888 3062 3453 3480 4825
Size: 744 722 129 687 174 391 27 1345
Cuts at: 4825 606
Size: 1084
Fragments arranged by size:
1345 1084 744 722 687 391 174 129
27
EagI C'GGCC_G
Cuts at: 2201 2888 2201
Size: 687 4616
EarI CTCTTCn'nnn_
Cuts at: 107 482 1009 1828 2209 3326 3536 107
Size: 375 527 819 381 1117 210 1874
Fragments arranged by size:
1874 1117 819 527 381 375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2069 3511 4253 4399 5113 2002
Size: 67 1442 742 146 714 2192
Fragments arranged by size:
2192 1442 742 714 146 67
EcoICRI GAG'CTC
Cuts at: 2315 2315
Size: 5303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 850 923 943 1276 1406 1852 1960 1972
Size: 72 73 20 333 130 446 108 12
Cuts at: 1972 1981 2023 2029 2056 2096 2136 3128 3560
Size: 9 42 6 27 40 40 992 432
Cuts at: 3560 3913 4575 778
Size: 353 662 1506
Fragments arranged by size:
1506 992 662 446 432 353 333 130
108 73 72 42 40 40 27 20
12 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 559 559
Size: 5303
EcoO109I rG'GnC_Cy
Cuts at: 1845 2178 2179 2442 1845
Size: 333 1 263 4706
Fragments arranged by size:
4706 333 263 1
EcoRI G'AATT_C
Cuts at: 2307 2307
Size: 5303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 5303
FspI TGC'GCA
Cuts at: 1345 3084 1345
Size: 1739 3564
HaeII r_GCGC'y
Cuts at: 1203 2047 2737 2985 4215 4986 1203
Size: 844 690 248 1230 771 1520
Fragments arranged by size:
1520 1230 844 771 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1077 1109 1414 1446 2071 2085 2103 2117 3145
Size: 32 305 32 625 14 18 14 1028
Cuts at: 3145 3175 3177 3207 1077
Size: 30 2 30 3173
Fragments arranged by size:
3173 1028 625 305 32 32 30 30
18 14 14 2
HincII GTy'rAC
Cuts at: 2342 2342
Size: 5303
KasI G'GCGC_C
Cuts at: 2733 2981 2733
Size: 248 5055
KpnI G_GTAC'C
Cuts at: 2323 2323
Size: 5303
MluI A'CGCG_T
Cuts at: 4736 4736
Size: 5303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1595 4062 4246 1205
Size: 390 2467 184 2262
Fragments arranged by size:
2467 2262 390 184
MscI TGG'CCA
Cuts at: 2074 3064 2074
Size: 990 4313
MslI CAynn'nnrTG
Cuts at: 1471 3292 3419 1471
Size: 1821 127 3355
Fragments arranged by size:
3355 1821 127
MspA1I CmG'CkG
Cuts at: 405 1138 1190 1592 1726 1959 2156 2414 2728
Size: 733 52 402 134 233 197 258 314
Cuts at: 2728 3088 3872 4117 405
Size: 360 784 245 1591
Fragments arranged by size:
1591 784 733 402 360 314 258 245
233 197 134 52
NaeI GCC'GGC
Cuts at: 1917 1975 3484 1917
Size: 58 1509 3736
Fragments arranged by size:
3736 1509 58
NarI GG'CG_CC
Cuts at: 2734 2982 2734
Size: 248 5055
NcoI C'CATG_G
Cuts at: 107 548 1658 107
Size: 441 1110 3752
Fragments arranged by size:
3752 1110 441
NgoMIV G'CCGG_C
Cuts at: 1915 1973 3482 1915
Size: 58 1509 3736
Fragments arranged by size:
3736 1509 58
NspI r_CATG'y
Cuts at: 995 2257 2356 2856 4459 995
Size: 1262 99 500 1603 1839
Fragments arranged by size:
1839 1603 1262 500 99
PasI CC'CwG_GG
Cuts at: 2064 2064
Size: 5303
PciI A'CATG_T
Cuts at: 991 4455 991
Size: 3464 1839
PflMI CCAn_nnn'nTGG
Cuts at: 2276 2276
Size: 5303
PfoI T'CCnGG_A
Cuts at: 149 1937 149
Size: 1788 3515
PmeI GTTT'AAAC
Cuts at: 2302 2302
Size: 5303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 119 151 683 715 1162 1194 3749 3781 119
Size: 32 532 32 447 32 2555 32 1641
Fragments arranged by size:
2555 1641 532 447 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 2020 2020
Size: 5303
PspOMI G'GGCC_C
Cuts at: 2178 2178
Size: 5303
PstI C_TGCA'G
Cuts at: 2350 3035 2350
Size: 685 4618
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5303
PvuII CAG'CTG
Cuts at: 1138 1959 2156 2728 3088 1138
Size: 821 197 572 360 3353
Fragments arranged by size:
3353 821 572 360 197
RsrII CG'GwC_CG
Cuts at: 3498 3498
Size: 5303
SacI G_AGCT'C
Cuts at: 2317 2317
Size: 5303
SacII CC_GC'GG
Cuts at: 1727 1727
Size: 5303
SalI G'TCGA_C
Cuts at: 2340 2340
Size: 5303
SapI GCTCTTCn'nnn_
Cuts at: 2209 3326 3536 2209
Size: 1117 210 3976
Fragments arranged by size:
3976 1117 210
SbfI CC_TGCA'GG
Cuts at: 2350 2350
Size: 5303
ScaI AGT'ACT
Cuts at: 4820 4820
Size: 5303
SexAI A'CCwGG_T
Cuts at: 554 554
Size: 5303
SfcI C'TryA_G
Cuts at: 33 849 1807 2346 3031 3999 4190 33
Size: 816 958 539 685 968 191 1146
Fragments arranged by size:
1146 968 958 816 685 539 191
SfoI GGC'GCC
Cuts at: 2735 2983 2735
Size: 248 5055
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5303
SmaI CCC'GGG
Cuts at: 1304 2231 2325 1304
Size: 927 94 4282
Fragments arranged by size:
4282 927 94
SmlI C'TyrA_G
Cuts at: 1639 2458 3373 4087 4349 1639
Size: 819 915 714 262 2593
Fragments arranged by size:
2593 915 819 714 262
SnaBI TAC'GTA
Cuts at: 719 719
Size: 5303
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5303
SphI G_CATG'C
Cuts at: 2356 2856 2356
Size: 500 4803
StuI AGG'CCT
Cuts at: 544 544
Size: 5303
StyI C'CwwG_G
Cuts at: 107 548 668 702 1043 1658 2270 2437 2791
Size: 441 120 34 341 615 612 167 354
Cuts at: 2791 107
Size: 2619
Fragments arranged by size:
2619 615 612 441 354 341 167 120
34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2886 3019 3478 3687 2886
Size: 133 459 209 4502
Fragments arranged by size:
4502 459 209 133
TatI w'GTAC_w
Cuts at: 1763 4818 1763
Size: 3055 2248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 1954 1986 4820 4852 865
Size: 32 1057 32 2834 32 1316
Fragments arranged by size:
2834 1316 1057 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 556 1615 3100 556
Size: 1059 1485 2759
Fragments arranged by size:
2759 1485 1059
XbaI T'CTAG_A
Cuts at: 2334 2334
Size: 5303
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1768 2798 1768
Size: 1030 4273
XmaI C'CCGG_G
Cuts at: 1302 2229 2323 1302
Size: 927 94 4282
Fragments arranged by size:
4282 927 94
XmnI GAAnn'nnTTC
Cuts at: 601 1148 601
Size: 547 4756
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PsiI PspXI PsrI SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI