Restriction map for
pF1KE1023
(Circular) (Six-base) MAPSORT of: pF1KE1023.seq Check: 2484 from: 1 to: 5435
pF1KE1023 5435 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2343 2343
Size: 5435
AatII G_ACGT'C
Cuts at: 1285 1285
Size: 5435
AccI GT'mk_AC
Cuts at: 623 2156 2473 623
Size: 1533 317 3585
Fragments arranged by size:
3585 1533 317
Acc65I G'GTAC_C
Cuts at: 2451 2451
Size: 5435
AclI AA'CG_TT
Cuts at: 5275 5275
Size: 5435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1048 3260 3692 4045 4707 844
Size: 204 2212 432 353 662 1572
Fragments arranged by size:
2212 1572 662 432 353 204
AflIII A'CryG_T
Cuts at: 2129 2787 4587 4868 2129
Size: 658 1800 281 2696
Fragments arranged by size:
2696 1800 658 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1073 1687 1073
Size: 614 4821
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 419 451 419
Size: 32 5403
AlwNI CAG_nnn'CTG
Cuts at: 1861 2232 2740 4178 1861
Size: 371 508 1438 3118
Fragments arranged by size:
3118 1438 508 371
ApaI G_GGCC'C
Cuts at: 397 581 1079 1310 397
Size: 184 498 231 4522
Fragments arranged by size:
4522 498 231 184
ApaLI G'TGCA_C
Cuts at: 4273 4273
Size: 5435
ApoI r'AATT_y
Cuts at: 662 2439 4788 5044 662
Size: 1777 2349 256 1053
Fragments arranged by size:
2349 1777 1053 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
AvaI C'yCGr_G
Cuts at: 2203 2282 2455 2203
Size: 79 173 5183
Fragments arranged by size:
5183 173 79
BamHI G'GATC_C
Cuts at: 2334 2460 2334
Size: 126 5309
BanI G'GyrC_C
Cuts at: 2186 2355 2451 2865 3113 3148 2186
Size: 169 96 414 248 35 4473
Fragments arranged by size:
4473 414 248 169 96 35
BanII G_rGCy'C
Cuts at: 185 397 535 581 656 1079 1310 1384 2095
Size: 212 138 46 75 423 231 74 711
Cuts at: 2095 2449 3479 185
Size: 354 1030 2141
Fragments arranged by size:
2141 1030 711 423 354 231 212 138
75 74 46
BbeI G_GCGC'C
Cuts at: 2869 3117 2869
Size: 248 5187
BbsI GAAGACnn'nnnn_
Cuts at: 311 311
Size: 5435
BbvCI CC'TCA_GC
Cuts at: 1854 2117 1854
Size: 263 5172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2045 2079 2045
Size: 34 5401
BciVI GTATCCnnnnn_n'
Cuts at: 3333 4389 5435 3333
Size: 1056 1046 3333
Fragments arranged by size:
3333 1056 1046
BclI T'GATC_A
Cuts at: 1667 2955 1667
Size: 1288 4147
BglI GCCn_nnn'nGGC
Cuts at: 1008 1008
Size: 5435
BglII A'GATC_T
Cuts at: 690 2950 4987 690
Size: 2260 2037 1138
Fragments arranged by size:
2260 2037 1138
BlpI GC'TnA_GC
Cuts at: 2547 2547
Size: 5435
Bme1580I G_kGCm'C
Cuts at: 397 581 1079 1310 2360 3060 3153 4277 397
Size: 184 498 231 1050 700 93 1124 1555
Fragments arranged by size:
1555 1124 1050 700 498 231 184 93
BmgBI CAC'GTC
Cuts at: 2130 2130
Size: 5435
BmrI ACTGGGnnnn_n'
Cuts at: 715 1079 1126 2647 2728 3063 5207 715
Size: 364 47 1521 81 335 2144 943
Fragments arranged by size:
2144 1521 943 364 335 81 47
BmtI G_CTAG'C
Cuts at: 2063 2063
Size: 5435
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1512 3802 1512
Size: 2290 3145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 520 1091 1677 2144 2367 178
Size: 342 571 586 467 223 3246
Fragments arranged by size:
3246 586 571 467 342 223
Bpu10I CC'TnA_GC
Cuts at: 757 1660 1854 2117 757
Size: 903 194 263 4075
Fragments arranged by size:
4075 903 263 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1409 2611 3490 4204 4502 715
Size: 694 1202 879 714 298 1648
Fragments arranged by size:
1648 1202 879 714 694 298
BsaI GGTCTCn'nnnn_
Cuts at: 5139 5139
Size: 5435
BsaAI yAC'GTr
Cuts at: 2788 2788
Size: 5435
BsaBI GATnn'nnATC
Cuts at: 6 2949 4986 6
Size: 2943 2037 455
Fragments arranged by size:
2943 2037 455
BsaHI Gr'CG_yC
Cuts at: 1282 2384 2866 3114 3816 5312 1282
Size: 1102 482 248 702 1496 1405
Fragments arranged by size:
1496 1405 1102 702 482 248
BsaWI w'CCGG_w
Cuts at: 2624 3145 3823 4234 4381 4655 2624
Size: 521 678 411 147 274 3404
Fragments arranged by size:
3404 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 306 664 864 1094 1842 1857 2283 306
Size: 358 200 230 748 15 426 3458
Fragments arranged by size:
3458 748 426 358 230 200 15
BseYI C'CCAG_C
Cuts at: 1339 1970 2075 2330 2860 3618 4283 4691 1339
Size: 631 105 255 530 758 665 408 2083
Fragments arranged by size:
2083 758 665 631 530 408 255 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 443
Size: 5435
BsiEI CG_ry'CG
Cuts at: 104 2977 3023 4253 104
Size: 2873 46 1230 1286
Fragments arranged by size:
2873 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 405 656 1384 2095 2449 3227 3417 4277 405
Size: 251 728 711 354 778 190 860 1563
Fragments arranged by size:
1563 860 778 728 711 354 251 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2120
Size: 5435
Bsp1286I G_dGCh'C
Cuts at: 185 397 405 535 581 656 1079 1310 1384
Size: 212 8 130 46 75 423 231 74
Cuts at: 1384 2095 2360 2449 3060 3153 3227 3417 3479
Size: 711 265 89 611 93 74 190 62
Cuts at: 3479 4277 185
Size: 798 1343
Fragments arranged by size:
1343 798 711 611 423 265 231 212
190 130 93 89 75 74 74 62
46 8
BspHI T'CATG_A
Cuts at: 2271 3867 2271
Size: 1596 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 1832 2343 2485 2699 3001 3382 423
Size: 1409 511 142 214 302 381 2476
Fragments arranged by size:
2476 1409 511 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 2362 3727 3781 5265 5430 50
Size: 2312 1365 54 1484 165 55
Fragments arranged by size:
2312 1484 1365 165 55 54
BsrDI GCAATG_nn'
Cuts at: 395 1299 2427 3347 395
Size: 904 1128 920 2483
Fragments arranged by size:
2483 1128 920 904
BsrFI r'CCGG_y
Cuts at: 615 3433 3614 615
Size: 2818 181 2436
Fragments arranged by size:
2818 2436 181
BssSI C'ACGA_G
Cuts at: 216 3706 4414 216
Size: 3490 708 1237
Fragments arranged by size:
3490 1237 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1959 2812 1959
Size: 853 4582
BstBI TT'CG_AA
Cuts at: 3796 3796
Size: 5435
BstXI CCAn_nnnn'nTGG
Cuts at: 847 847
Size: 5435
BstYI r'GATC_y
Cuts at: 1 690 863 2112 2334 2460 2927 2950 3285
Size: 689 173 1249 222 126 467 23 335
Cuts at: 3285 3531 3837 3849 3935 4987 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1249 1052 689 467 449 335 306 246
222 173 126 86 23 12
BtgI C'CryG_G
Cuts at: 107 161 1706 1871 107
Size: 54 1545 165 3671
Fragments arranged by size:
3671 1545 165 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3385 3566 3729 3385
Size: 181 163 5091
Fragments arranged by size:
5091 181 163
BtsI GCAGTG_nn'
Cuts at: 1162 1859 1877 2678 1162
Size: 697 18 801 3919
Fragments arranged by size:
3919 801 697 18
DraI TTT'AAA
Cuts at: 2434 2434
Size: 5435
DrdI GACnn_nn'nnGTC
Cuts at: 3141 4485 3141
Size: 1344 4091
EaeI y'GGCC_r
Cuts at: 483 1874 3020 3194 3585 3612 4957 483
Size: 1391 1146 174 391 27 1345 961
Fragments arranged by size:
1391 1345 1146 961 391 174 27
EagI C'GGCC_G
Cuts at: 3020 3020
Size: 5435
EarI CTCTTCn'nnn_
Cuts at: 716 3458 3668 716
Size: 2742 210 2483
Fragments arranged by size:
2742 2483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3643 4385 4531 5245 3643
Size: 742 146 714 3833
Fragments arranged by size:
3833 742 714 146
EcoICRI GAG'CTC
Cuts at: 654 1382 2093 2447 654
Size: 728 711 354 3642
Fragments arranged by size:
3642 728 711 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 520 844 1048 1091 1677 2144 2367 3260
Size: 342 324 204 43 586 467 223 893
Cuts at: 3260 3692 4045 4707 178
Size: 432 353 662 906
Fragments arranged by size:
906 893 662 586 467 432 353 342
324 223 204 43
EcoNI CCTnn'n_nnAGG
Cuts at: 1106 1106
Size: 5435
EcoO109I rG'GnC_Cy
Cuts at: 435 577 1075 1306 1765 2229 2574 435
Size: 142 498 231 459 464 345 3296
Fragments arranged by size:
3296 498 464 459 345 231 142
EcoRI G'AATT_C
Cuts at: 2439 2439
Size: 5435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3454 3454
Size: 5435
FspI TGC'GCA
Cuts at: 3216 3216
Size: 5435
HaeII r_GCGC'y
Cuts at: 2320 2869 3117 4347 5118 2320
Size: 549 248 1230 771 2637
Fragments arranged by size:
2637 1230 771 549 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 438 470 1428 1460 2395 2427 3277 3307 3309
Size: 32 958 32 935 32 850 30 2
Cuts at: 3309 3339 438
Size: 30 2534
Fragments arranged by size:
2534 958 935 850 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2474 2474
Size: 5435
HindIII A'AGCT_T
Cuts at: 2170 2170
Size: 5435
KasI G'GCGC_C
Cuts at: 2865 3113 2865
Size: 248 5187
KpnI G_GTAC'C
Cuts at: 2455 2455
Size: 5435
MluI A'CGCG_T
Cuts at: 4868 4868
Size: 5435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4194 4378 4194
Size: 184 5251
MscI TGG'CCA
Cuts at: 485 3196 485
Size: 2711 2724
MslI CAynn'nnrTG
Cuts at: 1669 3424 3551 1669
Size: 1755 127 3553
Fragments arranged by size:
3553 1755 127
MspA1I CmG'CkG
Cuts at: 1026 1158 1893 2330 2546 2860 3220 4004 4249
Size: 132 735 437 216 314 360 784 245
Cuts at: 4249 1026
Size: 2212
Fragments arranged by size:
2212 784 735 437 360 314 245 216
132
NaeI GCC'GGC
Cuts at: 617 3616 617
Size: 2999 2436
NarI GG'CG_CC
Cuts at: 2866 3114 2866
Size: 248 5187
NcoI C'CATG_G
Cuts at: 107 161 107
Size: 54 5381
NgoMIV G'CCGG_C
Cuts at: 615 3614 615
Size: 2999 2436
NheI G'CTAG_C
Cuts at: 2059 2059
Size: 5435
NspI r_CATG'y
Cuts at: 147 855 1218 2488 2988 4591 147
Size: 708 363 1270 500 1603 991
Fragments arranged by size:
1603 1270 991 708 500 363
PasI CC'CwG_GG
Cuts at: 1302 1462 2232 2321 1302
Size: 160 770 89 4416
Fragments arranged by size:
4416 770 160 89
PciI A'CATG_T
Cuts at: 4587 4587
Size: 5435
PflMI CCAn_nnn'nTGG
Cuts at: 2232 2232
Size: 5435
PfoI T'CCnGG_A
Cuts at: 194 1107 194
Size: 913 4522
PmeI GTTT'AAAC
Cuts at: 2434 2434
Size: 5435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 5403
PpuMI rG'GwC_Cy
Cuts at: 435 1765 435
Size: 1330 4105
PspOMI G'GGCC_C
Cuts at: 393 577 1075 1306 393
Size: 184 498 231 4522
Fragments arranged by size:
4522 498 231 184
PstI C_TGCA'G
Cuts at: 434 1829 1898 1952 2058 2482 3167 434
Size: 1395 69 54 106 424 685 2702
Fragments arranged by size:
2702 1395 685 424 106 69 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5435
PvuII CAG'CTG
Cuts at: 1026 1893 2330 2860 3220 1026
Size: 867 437 530 360 3241
Fragments arranged by size:
3241 867 530 437 360
RsrII CG'GwC_CG
Cuts at: 3630 3630
Size: 5435
SacI G_AGCT'C
Cuts at: 656 1384 2095 2449 656
Size: 728 711 354 3642
Fragments arranged by size:
3642 728 711 354
SalI G'TCGA_C
Cuts at: 2472 2472
Size: 5435
SanDI GG'GwC_CC
Cuts at: 1765 1765
Size: 5435
SapI GCTCTTCn'nnn_
Cuts at: 716 3458 3668 716
Size: 2742 210 2483
Fragments arranged by size:
2742 2483 210
SbfI CC_TGCA'GG
Cuts at: 2482 2482
Size: 5435
ScaI AGT'ACT
Cuts at: 414 4952 414
Size: 4538 897
SfcI C'TryA_G
Cuts at: 33 430 557 620 1825 1894 1948 2054 2478
Size: 397 127 63 1205 69 54 106 424
Cuts at: 2478 3163 4131 4322 33
Size: 685 968 191 1146
Fragments arranged by size:
1205 1146 968 685 424 397 191 127
106 69 63 54
SfoI GGC'GCC
Cuts at: 2867 3115 2867
Size: 248 5187
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5435
SmaI CCC'GGG
Cuts at: 2457 2457
Size: 5435
SmlI C'TyrA_G
Cuts at: 694 1424 2590 3505 4219 4481 694
Size: 730 1166 915 714 262 1648
Fragments arranged by size:
1648 1166 915 730 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5435
SphI G_CATG'C
Cuts at: 147 2488 2988 147
Size: 2341 500 2594
Fragments arranged by size:
2594 2341 500
StuI AGG'CCT
Cuts at: 363 1849 2344 363
Size: 1486 495 3454
Fragments arranged by size:
3454 1486 495
StyI C'CwwG_G
Cuts at: 107 161 2569 2923 107
Size: 54 2408 354 2619
Fragments arranged by size:
2619 2408 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1163 3018 3151 3610 3819 1163
Size: 1855 133 459 209 2779
Fragments arranged by size:
2779 1855 459 209 133
TatI w'GTAC_w
Cuts at: 412 4950 412
Size: 4538 897
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 848 880 4952 4984 848
Size: 32 4072 32 1299
Fragments arranged by size:
4072 1299 32 32
Tth111I GACn'n_nGTC
Cuts at: 3232 3232
Size: 5435
XbaI T'CTAG_A
Cuts at: 2466 2466
Size: 5435
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1004 2930 1004
Size: 1926 3509
XmaI C'CCGG_G
Cuts at: 2455 2455
Size: 5435
ZraI GAC'GTC
Cuts at: 1283 1283
Size: 5435
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AscI AvrII BaeI
BfrBI BsaXI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI