Restriction map for
pF1KE1026
(Circular) (Six-base) MAPSORT of: pF1KE1026.seq Check: 6085 from: 1 to: 6050
pF1KE1026 6050 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 699 948 507
Size: 192 249 5609
Fragments arranged by size:
5609 249 192
AatII G_ACGT'C
Cuts at: 1973 1973
Size: 6050
AccI GT'mk_AC
Cuts at: 124 1967 3088 124
Size: 1843 1121 3086
Fragments arranged by size:
3086 1843 1121
Acc65I G'GTAC_C
Cuts at: 700 3066 700
Size: 2366 3684
AclI AA'CG_TT
Cuts at: 5890 5890
Size: 6050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 3875 4307 4660 5322 446
Size: 3429 432 353 662 1174
Fragments arranged by size:
3429 1174 662 432 353
AflIII A'CryG_T
Cuts at: 578 972 974 1184 2360 2762 3402 5202 5483
Size: 394 2 210 1176 402 640 1800 281
Cuts at: 5483 578
Size: 1145
Fragments arranged by size:
1800 1176 1145 640 402 394 281 210
2
AgeI A'CCGG_T
Cuts at: 370 370
Size: 6050
AleI CACnn'nnGTG
Cuts at: 1345 1996 1345
Size: 651 5399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1909 1909
Size: 6050
AlwNI CAG_nnn'CTG
Cuts at: 540 639 801 900 1521 1938 2178 2349 2430
Size: 99 162 99 621 417 240 171 81
Cuts at: 2430 2517 3033 3355 4793 540
Size: 87 516 322 1438 1797
Fragments arranged by size:
1797 1438 621 516 417 322 240 171
162 99 99 87 81
ApaI G_GGCC'C
Cuts at: 777 1913 2968 777
Size: 1136 1055 3859
Fragments arranged by size:
3859 1136 1055
ApaLI G'TGCA_C
Cuts at: 374 712 4888 374
Size: 338 4176 1536
Fragments arranged by size:
4176 1536 338
ApoI r'AATT_y
Cuts at: 3054 5403 5659 3054
Size: 2349 256 3445
Fragments arranged by size:
3445 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 1060 1912 2296 3070 1060
Size: 852 384 774 4040
Fragments arranged by size:
4040 852 774 384
BamHI G'GATC_C
Cuts at: 3075 3075
Size: 6050
BanI G'GyrC_C
Cuts at: 216 430 526 700 960 1339 1597 2052 2332
Size: 214 96 174 260 379 258 455 280
Cuts at: 2332 3066 3480 3728 3763 216
Size: 734 414 248 35 2503
Fragments arranged by size:
2503 734 455 414 379 280 260 258
248 214 174 96 35
BanII G_rGCy'C
Cuts at: 289 341 777 1529 1874 1913 2132 2968 3064
Size: 52 436 752 345 39 219 836 96
Cuts at: 3064 4094 289
Size: 1030 2245
Fragments arranged by size:
2245 1030 836 752 436 345 219 96
52 39
BbeI G_GCGC'C
Cuts at: 2056 2336 3484 3732 2056
Size: 280 1148 248 4374
Fragments arranged by size:
4374 1148 280 248
BbsI GAAGACnn'nnnn_
Cuts at: 1560 2020 1560
Size: 460 5590
BbvCI CC'TCA_GC
Cuts at: 257 1454 2035 257
Size: 1197 581 4272
Fragments arranged by size:
4272 1197 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1182 1216 1182
Size: 34 6016
BciVI GTATCCnnnnn_n'
Cuts at: 3948 5004 6050 3948
Size: 1056 1046 3948
Fragments arranged by size:
3948 1056 1046
BclI T'GATC_A
Cuts at: 3570 3570
Size: 6050
BglI GCCn_nnn'nGGC
Cuts at: 439 491 1595 2842 2995 439
Size: 52 1104 1247 153 3494
Fragments arranged by size:
3494 1247 1104 153 52
BglII A'GATC_T
Cuts at: 847 3565 5602 847
Size: 2718 2037 1295
Fragments arranged by size:
2718 2037 1295
BlpI GC'TnA_GC
Cuts at: 2431 3162 2431
Size: 731 5319
Bme1580I G_kGCm'C
Cuts at: 378 529 614 716 777 787 965 1600 1913
Size: 151 85 102 61 10 178 635 313
Cuts at: 1913 2968 3675 3768 4892 378
Size: 1055 707 93 1124 1536
Fragments arranged by size:
1536 1124 1055 707 635 313 178 151
102 93 85 61 10
BmgBI CAC'GTC
Cuts at: 2202 2363 2202
Size: 161 5889
BmrI ACTGGGnnnn_n'
Cuts at: 1232 2039 2213 3262 3343 3678 5822 1232
Size: 807 174 1049 81 335 2144 1460
Fragments arranged by size:
2144 1460 1049 807 335 174 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2493 4417 2493
Size: 1924 4126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 901 937 966 1543 1864 2043 2281 2539
Size: 327 36 29 577 321 179 238 258
Cuts at: 2539 2581 2998 3024 574
Size: 42 417 26 3600
Fragments arranged by size:
3600 577 417 327 321 258 238 179
42 36 29 26
Bpu10I CC'TnA_GC
Cuts at: 257 1454 2035 257
Size: 1197 581 4272
Fragments arranged by size:
4272 1197 581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1711 2590 2644 3226 4105 4819 5117 920
Size: 791 879 54 582 879 714 298 1853
Fragments arranged by size:
1853 879 879 791 714 582 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 687 2800 2965 5754 687
Size: 2113 165 2789 983
Fragments arranged by size:
2789 2113 983 165
BsaAI yAC'GTr
Cuts at: 330 579 930 975 1185 1347 3403 330
Size: 249 351 45 210 162 2056 2977
Fragments arranged by size:
2977 2056 351 249 210 162 45
BsaBI GATnn'nnATC
Cuts at: 6 3564 5601 6
Size: 3558 2037 455
Fragments arranged by size:
3558 2037 455
BsaHI Gr'CG_yC
Cuts at: 815 1066 1904 1970 2053 2333 3481 3729 4431
Size: 251 838 66 83 280 1148 248 702
Cuts at: 4431 5927 815
Size: 1496 938
Fragments arranged by size:
1496 1148 938 838 702 280 251 248
83 66
BsaWI w'CCGG_w
Cuts at: 370 1315 2240 3239 3760 4438 4849 4996 5270
Size: 945 925 999 521 678 411 147 274
Cuts at: 5270 370
Size: 1150
Fragments arranged by size:
1150 999 945 925 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 1525 1555 1993 2023 964
Size: 30 531 30 438 30 4991
Fragments arranged by size:
4991 531 438 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2104 2314 2647 2896 2908 2911 2914 2104
Size: 210 333 249 12 3 3 5240
Fragments arranged by size:
5240 333 249 210 12 3 3
BseYI C'CCAG_C
Cuts at: 155 182 188 210 221 290 398 530 614
Size: 27 6 22 11 69 108 132 84
Cuts at: 614 704 1228 1368 2961 3475 4233 4898 5306
Size: 90 524 140 1593 514 758 665 408
Cuts at: 5306 155
Size: 899
Fragments arranged by size:
1593 899 758 665 524 514 408 140
132 108 90 84 69 27 22 11
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 541 580 667 760 913 928 964 1012
Size: 164 39 87 93 153 15 36 48
Cuts at: 1012 1486 1837 2485 2530 3046 377
Size: 474 351 648 45 516 3381
Fragments arranged by size:
3381 648 516 474 351 164 153 93
87 48 45 39 36 15
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4868 104
Size: 3488 46 1230 1286
Fragments arranged by size:
3488 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 341 378 716 1480 1789 1874 2114 2138 2606
Size: 37 338 764 309 85 240 24 468
Cuts at: 2606 2783 3064 3842 4032 4892 341
Size: 177 281 778 190 860 1499
Fragments arranged by size:
1499 860 778 764 468 338 309 281
240 190 177 85 37 24
BsmBI CGTCTCn'nnnn_
Cuts at: 1057 1441 1996 1057
Size: 384 555 5111
Fragments arranged by size:
5111 555 384
Bsp1286I G_dGCh'C
Cuts at: 289 341 378 529 614 716 777 787 965
Size: 52 37 151 85 102 61 10 178
Cuts at: 965 1480 1529 1600 1789 1874 1913 2114 2132
Size: 515 49 71 189 85 39 201 18
Cuts at: 2132 2138 2606 2783 2968 3064 3675 3768 3842
Size: 6 468 177 185 96 611 93 74
Cuts at: 3842 4032 4094 4892 289
Size: 190 62 798 1447
Fragments arranged by size:
1447 798 611 515 468 201 190 189
185 178 177 151 102 96 93 85
85 74 71 62 61 52 49 39
37 18 10 6
BspEI T'CCGG_A
Cuts at: 2240 2240
Size: 6050
BspHI T'CATG_A
Cuts at: 4482 4482
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 507 699 795 948 1991 3100 3314 3616 3997
Size: 192 96 153 1043 1109 214 302 381
Cuts at: 3997 507
Size: 2560
Fragments arranged by size:
2560 1109 1043 381 302 214 192 153
96
BsrBI CCG'CTC
Cuts at: 50 1562 4342 4396 5880 6045 50
Size: 1512 2780 54 1484 165 55
Fragments arranged by size:
2780 1512 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3962 3962
Size: 6050
BsrFI r'CCGG_y
Cuts at: 370 490 735 2626 2985 4048 4229 370
Size: 120 245 1891 359 1063 181 2191
Fragments arranged by size:
2191 1891 1063 359 245 181 120
BsrGI T'GTAC_A
Cuts at: 1073 2067 1073
Size: 994 5056
BssHII G'CGCG_C
Cuts at: 2389 2391 3010 2389
Size: 2 619 5429
Fragments arranged by size:
5429 619 2
BssSI C'ACGA_G
Cuts at: 2478 4321 5029 2478
Size: 1843 708 3499
Fragments arranged by size:
3499 1843 708
BstAPI GCAn_nnn'nTGC
Cuts at: 540 2349 3033 3427 540
Size: 1809 684 394 3163
Fragments arranged by size:
3163 1809 684 394
BstBI TT'CG_AA
Cuts at: 4411 4411
Size: 6050
BstXI CCAn_nnnn'nTGG
Cuts at: 1627 1764 2259 1627
Size: 137 495 5418
Fragments arranged by size:
5418 495 137
BstYI r'GATC_y
Cuts at: 1 847 3075 3542 3565 3900 4146 4452 4464
Size: 846 2228 467 23 335 246 306 12
Cuts at: 4464 4550 5602 1
Size: 86 1052 449
Fragments arranged by size:
2228 1052 846 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 125 125
Size: 6050
Bsu36I CC'TnA_GG
Cuts at: 1898 1898
Size: 6050
BtgI C'CryG_G
Cuts at: 107 696 779 810 1418 1471 2282 2324 2681
Size: 589 83 31 608 53 811 42 357
Cuts at: 2681 107
Size: 3476
Fragments arranged by size:
3476 811 608 589 357 83 53 42
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1025 2799 4000 4181 4344 1025
Size: 1774 1201 181 163 2731
Fragments arranged by size:
2731 1774 1201 181 163
BtsI GCAGTG_nn'
Cuts at: 1170 1266 1311 1380 1503 2347 2572 3293 1170
Size: 96 45 69 123 844 225 721 3927
Fragments arranged by size:
3927 844 721 225 123 96 69 45
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DraIII CAC_nnn'GTG
Cuts at: 313 313
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 3756 5100 3756
Size: 1344 4706
EaeI y'GGCC_r
Cuts at: 315 675 1025 1118 1157 1688 2321 2843 2987
Size: 360 350 93 39 531 633 522 144
Cuts at: 2987 3635 3809 4200 4227 5572 315
Size: 648 174 391 27 1345 793
Fragments arranged by size:
1345 793 648 633 531 522 391 360
350 174 144 93 39 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 629 759 2656 2885 3025 4073 4283 629
Size: 130 1897 229 140 1048 210 2396
Fragments arranged by size:
2396 1897 1048 229 210 140 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1203 4258 5000 5146 5860 1203
Size: 3055 742 146 714 1393
Fragments arranged by size:
3055 1393 742 714 146
EcoICRI GAG'CTC
Cuts at: 339 1872 3062 339
Size: 1533 1190 3327
Fragments arranged by size:
3327 1533 1190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 574 901 937 966 1543 1864 2043 2281
Size: 128 327 36 29 577 321 179 238
Cuts at: 2281 2539 2581 2998 3024 3875 4307 4660 5322
Size: 258 42 417 26 851 432 353 662
Cuts at: 5322 446
Size: 1174
Fragments arranged by size:
1174 851 662 577 432 417 353 327
321 258 238 179 128 42 36 29
26
EcoNI CCTnn'n_nnAGG
Cuts at: 2022 2380 2022
Size: 358 5692
EcoO109I rG'GnC_Cy
Cuts at: 168 773 774 912 1935 2175 2694 3189 168
Size: 605 1 138 1023 240 519 495 3029
Fragments arranged by size:
3029 1023 605 519 495 240 138 1
EcoRI G'AATT_C
Cuts at: 3054 3054
Size: 6050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2685 4069 2685
Size: 1384 4666
FspI TGC'GCA
Cuts at: 1636 2707 3831 1636
Size: 1071 1124 3855
Fragments arranged by size:
3855 1124 1071
HaeII r_GCGC'y
Cuts at: 877 1811 2056 2336 2358 2627 2714 3034 3484
Size: 934 245 280 22 269 87 320 450
Cuts at: 3484 3732 4962 5733 877
Size: 248 1230 771 1194
Fragments arranged by size:
1230 1194 934 771 450 320 280 269
248 245 87 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 439 471 839 871 3892 3922 3924 3954 439
Size: 32 368 32 3021 30 2 30 2535
Fragments arranged by size:
3021 2535 368 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1533 1968 2289 3089 1533
Size: 435 321 800 4494
Fragments arranged by size:
4494 800 435 321
KasI G'GCGC_C
Cuts at: 2052 2332 3480 3728 2052
Size: 280 1148 248 4374
Fragments arranged by size:
4374 1148 280 248
KpnI G_GTAC'C
Cuts at: 704 3070 704
Size: 2366 3684
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 1778 4809 4993 437
Size: 1341 3031 184 1494
Fragments arranged by size:
3031 1494 1341 184
MscI TGG'CCA
Cuts at: 317 677 1159 1690 2845 3811 317
Size: 360 482 531 1155 966 2556
Fragments arranged by size:
2556 1155 966 531 482 360
MslI CAynn'nnrTG
Cuts at: 607 1177 1183 1345 1625 1690 1996 2107 4039
Size: 570 6 162 280 65 306 111 1932
Cuts at: 4039 4166 607
Size: 127 2491
Fragments arranged by size:
2491 1932 570 306 280 162 127 111
65 6
MspA1I CmG'CkG
Cuts at: 214 244 537 594 801 900 1071 1247 1331
Size: 30 293 57 207 99 171 176 84
Cuts at: 1331 2061 2326 2492 2514 3161 3475 3835 4619
Size: 730 265 166 22 647 314 360 784
Cuts at: 4619 4864 214
Size: 245 1400
Fragments arranged by size:
1400 784 730 647 360 314 293 265
245 207 176 171 166 99 84 57
30 22
NaeI GCC'GGC
Cuts at: 2628 2987 4231 2628
Size: 359 1244 4447
Fragments arranged by size:
4447 1244 359
NarI GG'CG_CC
Cuts at: 2053 2333 3481 3729 2053
Size: 280 1148 248 4374
Fragments arranged by size:
4374 1148 280 248
NcoI C'CATG_G
Cuts at: 107 696 2282 2681 107
Size: 589 1586 399 3476
Fragments arranged by size:
3476 1586 589 399
NgoMIV G'CCGG_C
Cuts at: 2626 2985 4229 2626
Size: 359 1244 4447
Fragments arranged by size:
4447 1244 359
NspI r_CATG'y
Cuts at: 1368 2766 3103 3603 5206 1368
Size: 1398 337 500 1603 2212
Fragments arranged by size:
2212 1603 1398 500 337
PciI A'CATG_T
Cuts at: 2762 5202 2762
Size: 2440 3610
PflMI CCAn_nnn'nTGG
Cuts at: 313 1155 1743 2178 2283 313
Size: 842 588 435 105 4080
Fragments arranged by size:
4080 842 588 435 105
PfoI T'CCnGG_A
Cuts at: 1433 2690 1433
Size: 1257 4793
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PmlI CAC'GTG
Cuts at: 579 930 975 579
Size: 351 45 5654
Fragments arranged by size:
5654 351 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 1935 2694 1935
Size: 759 5291
PspOMI G'GGCC_C
Cuts at: 773 1909 2964 773
Size: 1136 1055 3859
Fragments arranged by size:
3859 1136 1055
PstI C_TGCA'G
Cuts at: 284 806 1988 2405 2546 3097 3782 284
Size: 522 1182 417 141 551 685 2552
Fragments arranged by size:
2552 1182 685 551 522 417 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 594 801 3475 3835 594
Size: 207 2674 360 2809
Fragments arranged by size:
2809 2674 360 207
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 341 1874 3064 341
Size: 1533 1190 3327
Fragments arranged by size:
3327 1533 1190
SacII CC_GC'GG
Cuts at: 2327 2327
Size: 6050
SalI G'TCGA_C
Cuts at: 1966 3087 1966
Size: 1121 4929
SapI GCTCTTCn'nnn_
Cuts at: 629 759 2656 4073 4283 629
Size: 130 1897 1417 210 2396
Fragments arranged by size:
2396 1897 1417 210 130
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 5567 5567
Size: 6050
SfcI C'TryA_G
Cuts at: 33 133 280 802 1984 2401 2542 2673 3093
Size: 100 147 522 1182 417 141 131 420
Cuts at: 3093 3778 4746 4937 33
Size: 685 968 191 1146
Fragments arranged by size:
1182 1146 968 685 522 420 417 191
147 141 131 100
SfoI GGC'GCC
Cuts at: 2054 2334 3482 3730 2054
Size: 280 1148 248 4374
Fragments arranged by size:
4374 1148 280 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SgrAI Cr'CCGG_yG
Cuts at: 735 735
Size: 6050
SmaI CCC'GGG
Cuts at: 1914 3072 1914
Size: 1158 4892
SmlI C'TyrA_G
Cuts at: 935 1726 2605 2659 3205 4120 4834 5096 935
Size: 791 879 54 546 915 714 262 1889
Fragments arranged by size:
1889 915 879 791 714 546 262 54
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
StuI AGG'CCT
Cuts at: 2476 2476
Size: 6050
StyI C'CwwG_G
Cuts at: 107 696 1353 2282 2636 2681 2798 3184 3538
Size: 589 657 929 354 45 117 386 354
Cuts at: 3538 107
Size: 2619
Fragments arranged by size:
2619 929 657 589 386 354 354 117
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 393 544 853 1138 2290 2472 3633 3766
Size: 141 151 309 285 1152 182 1161 133
Cuts at: 3766 4225 4434 252
Size: 459 209 1868
Fragments arranged by size:
1868 1161 1152 459 309 285 209 182
151 141 133
TatI w'GTAC_w
Cuts at: 404 977 1073 2067 2759 2765 5565 404
Size: 573 96 994 692 6 2800 889
Fragments arranged by size:
2800 994 889 692 573 96 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 1993 2025 5567 5599 964
Size: 32 997 32 3542 32 1415
Fragments arranged by size:
3542 1415 997 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 453 3847 453
Size: 3394 2656
XbaI T'CTAG_A
Cuts at: 3081 3081
Size: 6050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 636 1740 2103 2820 3545 636
Size: 1104 363 717 725 3141
Fragments arranged by size:
3141 1104 725 717 363
XmaI C'CCGG_G
Cuts at: 1912 3070 1912
Size: 1158 4892
XmnI GAAnn'nnTTC
Cuts at: 2017 2017
Size: 6050
ZraI GAC'GTC
Cuts at: 1971 1971
Size: 6050
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BstEII ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI