Restriction map for
pF1KE1028
(Circular) (Six-base) MAPSORT of: pF1KE1028.seq Check: 5354 from: 1 to: 6764
pF1KE1028 6764 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 111 111
Size: 6764
AccI GT'mk_AC
Cuts at: 2422 3802 2422
Size: 1380 5384
Acc65I G'GTAC_C
Cuts at: 3780 3780
Size: 6764
AclI AA'CG_TT
Cuts at: 1080 6604 1080
Size: 5524 1240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1397 2512 2782 3210 4589 5021 5374 6036
Size: 1238 1115 270 428 1379 432 353 662
Cuts at: 6036 159
Size: 887
Fragments arranged by size:
1379 1238 1115 887 662 432 428 353
270
AfeI AGC'GCT
Cuts at: 489 2815 489
Size: 2326 4438
AflIII A'CryG_T
Cuts at: 1980 4116 5916 6197 1980
Size: 2136 1800 281 2547
Fragments arranged by size:
2547 2136 1800 281
AgeI A'CCGG_T
Cuts at: 368 368
Size: 6764
AleI CACnn'nnGTG
Cuts at: 1753 1753
Size: 6764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1571 1571
Size: 6764
AlwNI CAG_nnn'CTG
Cuts at: 1104 2782 2985 3552 4069 5507 1104
Size: 1678 203 567 517 1438 2361
Fragments arranged by size:
2361 1678 1438 567 517 203
ApaLI G'TGCA_C
Cuts at: 5602 5602
Size: 6764
ApoI r'AATT_y
Cuts at: 947 1156 2287 2933 3081 3661 3768 6117 6373
Size: 209 1131 646 148 580 107 2349 256
Cuts at: 6373 947
Size: 1338
Fragments arranged by size:
2349 1338 1131 646 580 256 209 148
107
AseI AT'TA_AT
Cuts at: 20 1325 2228 20
Size: 1305 903 4556
Fragments arranged by size:
4556 1305 903
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6764
AvaI C'yCGr_G
Cuts at: 1445 2585 3784 1445
Size: 1140 1199 4425
Fragments arranged by size:
4425 1199 1140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 590 623 590
Size: 33 6731
BamHI G'GATC_C
Cuts at: 3789 3789
Size: 6764
BanI G'GyrC_C
Cuts at: 415 3780 4194 4442 4477 415
Size: 3365 414 248 35 2702
Fragments arranged by size:
3365 2702 414 248 35
BanII G_rGCy'C
Cuts at: 774 3778 4808 774
Size: 3004 1030 2730
Fragments arranged by size:
3004 2730 1030
BbeI G_GCGC'C
Cuts at: 4198 4446 4198
Size: 248 6516
BbsI GAAGACnn'nnnn_
Cuts at: 1376 1376
Size: 6764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 796 830 796
Size: 34 6730
BciVI GTATCCnnnnn_n'
Cuts at: 1890 4662 5718 6764 1890
Size: 2772 1056 1046 1890
Fragments arranged by size:
2772 1890 1056 1046
BclI T'GATC_A
Cuts at: 1934 2241 3707 4284 1934
Size: 307 1466 577 4414
Fragments arranged by size:
4414 1466 577 307
BglII A'GATC_T
Cuts at: 1572 4279 6316 1572
Size: 2707 2037 2020
Fragments arranged by size:
2707 2037 2020
BlpI GC'TnA_GC
Cuts at: 839 884 3560 3876 839
Size: 45 2676 316 3727
Fragments arranged by size:
3727 2676 316 45
Bme1580I G_kGCm'C
Cuts at: 4389 4482 5606 4389
Size: 93 1124 5547
Fragments arranged by size:
5547 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2111 2458 3976 4057 4392 6536 2111
Size: 347 1518 81 335 2144 2339
Fragments arranged by size:
2339 2144 1518 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5131 5131
Size: 6764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1874 3410 1420
Size: 454 1536 4774
Fragments arranged by size:
4774 1536 454
Bpu10I CC'TnA_GC
Cuts at: 2372 2372
Size: 6764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1288 3034 3293 3667 3940 4819 5533 5831
Size: 98 1746 259 374 273 879 714 298
Cuts at: 5831 1190
Size: 2123
Fragments arranged by size:
2123 1746 879 714 374 298 273 259
98
BsaI GGTCTCn'nnnn_
Cuts at: 2266 6468 2266
Size: 4202 2562
BsaAI yAC'GTr
Cuts at: 4117 4117
Size: 6764
BsaBI GATnn'nnATC
Cuts at: 6 4278 6315 6
Size: 4272 2037 455
Fragments arranged by size:
4272 2037 455
BsaHI Gr'CG_yC
Cuts at: 4195 4443 5145 6641 4195
Size: 248 702 1496 4318
Fragments arranged by size:
4318 1496 702 248
BsaWI w'CCGG_w
Cuts at: 368 3953 4474 5152 5563 5710 5984 368
Size: 3585 521 678 411 147 274 1148
Fragments arranged by size:
3585 1148 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2027 2276 4189 4947 5612 6020 2027
Size: 249 1913 758 665 408 2771
Fragments arranged by size:
2771 1913 758 665 408 249
BsiEI CG_ry'CG
Cuts at: 104 4306 4352 5582 104
Size: 4202 46 1230 1286
Fragments arranged by size:
4202 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2097 3778 4556 4746 5606 2097
Size: 1681 778 190 860 3255
Fragments arranged by size:
3255 1681 860 778 190
BsmI GAATG_Cn'
Cuts at: 1542 1542
Size: 6764
BsmBI CGTCTCn'nnnn_
Cuts at: 759 759
Size: 6764
Bsp1286I G_dGCh'C
Cuts at: 774 2097 3778 4389 4482 4556 4746 4808 5606
Size: 1323 1681 611 93 74 190 62 798
Cuts at: 5606 774
Size: 1932
Fragments arranged by size:
1932 1681 1323 798 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 2856 5196 2856
Size: 2340 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 111 2213 3814 4028 4330 4711 111
Size: 2102 1601 214 302 381 2164
Fragments arranged by size:
2164 2102 1601 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5056 5110 6594 6759 50
Size: 5006 54 1484 165 55
Fragments arranged by size:
5006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4676 4676
Size: 6764
BsrFI r'CCGG_y
Cuts at: 368 4762 4943 368
Size: 4394 181 2189
Fragments arranged by size:
4394 2189 181
BsrGI T'GTAC_A
Cuts at: 110 3603 110
Size: 3493 3271
BssSI C'ACGA_G
Cuts at: 320 812 5035 5743 320
Size: 492 4223 708 1341
Fragments arranged by size:
4223 1341 708 492
BstAPI GCAn_nnn'nTGC
Cuts at: 1104 1558 2898 4141 1104
Size: 454 1340 1243 3727
Fragments arranged by size:
3727 1340 1243 454
BstBI TT'CG_AA
Cuts at: 141 402 3006 5125 141
Size: 261 2604 2119 1780
Fragments arranged by size:
2604 2119 1780 261
BstEII G'GTnAC_C
Cuts at: 371 2071 371
Size: 1700 5064
BstXI CCAn_nnnn'nTGG
Cuts at: 3388 3388
Size: 6764
BstYI r'GATC_y
Cuts at: 1 168 211 1227 1425 1572 3789 4256 4279
Size: 167 43 1016 198 147 2217 467 23
Cuts at: 4279 4614 4860 5166 5178 5264 6316 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2217 1052 1016 467 449 335 306 246
198 167 147 86 43 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4714 4895 5058 4714
Size: 181 163 6420
Fragments arranged by size:
6420 181 163
BtsI GCAGTG_nn'
Cuts at: 1763 3025 3369 4007 1763
Size: 1262 344 638 4520
Fragments arranged by size:
4520 1262 638 344
ClaI AT'CG_AT
Cuts at: 380 3145 380
Size: 2765 3999
DraI TTT'AAA
Cuts at: 1625 2292 3763 1625
Size: 667 1471 4626
Fragments arranged by size:
4626 1471 667
DraIII CAC_nnn'GTG
Cuts at: 3757 3757
Size: 6764
DrdI GACnn_nn'nnGTC
Cuts at: 4470 5814 4470
Size: 1344 5420
EaeI y'GGCC_r
Cuts at: 3722 4349 4523 4914 4941 6286 3722
Size: 627 174 391 27 1345 4200
Fragments arranged by size:
4200 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 4349 4349
Size: 6764
EarI CTCTTCn'nnn_
Cuts at: 399 692 1526 2335 2485 2797 2912 3116 4787
Size: 293 834 809 150 312 115 204 1671
Cuts at: 4787 4997 399
Size: 210 2166
Fragments arranged by size:
2166 1671 834 809 312 293 210 204
150 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4972 5714 5860 6574 4972
Size: 742 146 714 5162
Fragments arranged by size:
5162 742 714 146
EcoICRI GAG'CTC
Cuts at: 3776 3776
Size: 6764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1397 1420 1874 2512 2782 3210 3410 4589
Size: 1238 23 454 638 270 428 200 1179
Cuts at: 4589 5021 5374 6036 159
Size: 432 353 662 887
Fragments arranged by size:
1238 1179 887 662 638 454 432 428
353 270 200 23
EcoO109I rG'GnC_Cy
Cuts at: 3903 3903
Size: 6764
EcoRI G'AATT_C
Cuts at: 3768 3768
Size: 6764
EcoRV GAT'ATC
Cuts at: 915 915
Size: 6764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 415 527 994 4783 415
Size: 112 467 3789 2396
Fragments arranged by size:
3789 2396 467 112
FspI TGC'GCA
Cuts at: 2209 4545 2209
Size: 2336 4428
HaeII r_GCGC'y
Cuts at: 491 2817 4198 4446 5676 6447 491
Size: 2326 1381 248 1230 771 808
Fragments arranged by size:
2326 1381 1230 808 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1938 1970 2871 2903 4606 4636 4638 4668 1938
Size: 32 901 32 1703 30 2 30 4034
Fragments arranged by size:
4034 1703 901 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2524 3054 3200 3378 3803 2524
Size: 530 146 178 425 5485
Fragments arranged by size:
5485 530 425 178 146
HindIII A'AGCT_T
Cuts at: 1970 2403 1970
Size: 433 6331
HpaI GTT'AAC
Cuts at: 3054 3200 3054
Size: 146 6618
KasI G'GCGC_C
Cuts at: 4194 4442 4194
Size: 248 6516
KpnI G_GTAC'C
Cuts at: 3784 3784
Size: 6764
MfeI C'AATT_G
Cuts at: 161 161
Size: 6764
MluI A'CGCG_T
Cuts at: 6197 6197
Size: 6764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1001 3375 5523 5707 1001
Size: 2374 2148 184 2058
Fragments arranged by size:
2374 2148 2058 184
MscI TGG'CCA
Cuts at: 3724 4525 3724
Size: 801 5963
MslI CAynn'nnrTG
Cuts at: 1753 2090 2843 3701 4753 4880 1753
Size: 337 753 858 1052 127 3637
Fragments arranged by size:
3637 1052 858 753 337 127
MspA1I CmG'CkG
Cuts at: 288 2031 3655 3875 4189 4549 5333 5578 288
Size: 1743 1624 220 314 360 784 245 1474
Fragments arranged by size:
1743 1624 1474 784 360 314 245 220
NaeI GCC'GGC
Cuts at: 4945 4945
Size: 6764
NarI GG'CG_CC
Cuts at: 4195 4443 4195
Size: 248 6516
NdeI CA'TA_TG
Cuts at: 2840 2840
Size: 6764
NgoMIV G'CCGG_C
Cuts at: 4943 4943
Size: 6764
NspI r_CATG'y
Cuts at: 1690 1754 1984 2413 2901 3610 3817 4317 5920
Size: 64 230 429 488 709 207 500 1603
Cuts at: 5920 1690
Size: 2534
Fragments arranged by size:
2534 1603 709 500 488 429 230 207
64
PciI A'CATG_T
Cuts at: 1980 5916 1980
Size: 3936 2828
PflMI CCAn_nnn'nTGG
Cuts at: 3552 3552
Size: 6764
PfoI T'CCnGG_A
Cuts at: 1851 1851
Size: 6764
PmeI GTTT'AAAC
Cuts at: 3763 3763
Size: 6764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 6732
PspXI vC'TCGA_Gb
Cuts at: 1445 2585 1445
Size: 1140 5624
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 6732
PstI C_TGCA'G
Cuts at: 3811 4496 3811
Size: 685 6079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6764
PvuII CAG'CTG
Cuts at: 2031 3655 4189 4549 2031
Size: 1624 534 360 4246
Fragments arranged by size:
4246 1624 534 360
RsrII CG'GwC_CG
Cuts at: 4959 4959
Size: 6764
SacI G_AGCT'C
Cuts at: 3778 3778
Size: 6764
SalI G'TCGA_C
Cuts at: 3801 3801
Size: 6764
SapI GCTCTTCn'nnn_
Cuts at: 4787 4997 4787
Size: 210 6554
SbfI CC_TGCA'GG
Cuts at: 3811 3811
Size: 6764
ScaI AGT'ACT
Cuts at: 6281 6281
Size: 6764
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 6764
SfcI C'TryA_G
Cuts at: 33 3807 4492 5460 5651 33
Size: 3774 685 968 191 1146
Fragments arranged by size:
3774 1146 968 685 191
SfoI GGC'GCC
Cuts at: 4196 4444 4196
Size: 248 6516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6764
SmaI CCC'GGG
Cuts at: 3786 3786
Size: 6764
SmlI C'TyrA_G
Cuts at: 1205 1303 1445 2585 3013 3308 3646 3919 4834
Size: 98 142 1140 428 295 338 273 915
Cuts at: 4834 5548 5810 1205
Size: 714 262 2159
Fragments arranged by size:
2159 1140 915 714 428 338 295 273
262 142 98
SpeI A'CTAG_T
Cuts at: 67 850 67
Size: 783 5981
SphI G_CATG'C
Cuts at: 2413 2901 3817 4317 2413
Size: 488 916 500 4860
Fragments arranged by size:
4860 916 500 488
SspI AAT'ATT
Cuts at: 1471 2217 1471
Size: 746 6018
StuI AGG'CCT
Cuts at: 3636 3636
Size: 6764
StyI C'CwwG_G
Cuts at: 1034 3470 3898 4252 1034
Size: 2436 428 354 3546
Fragments arranged by size:
3546 2436 428 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4347 4480 4939 5148 4347
Size: 133 459 209 5963
Fragments arranged by size:
5963 459 209 133
TatI w'GTAC_w
Cuts at: 110 3041 3165 3603 6279 110
Size: 2931 124 438 2676 595
Fragments arranged by size:
2931 2676 595 438 124
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 6281 6313 3389
Size: 32 2860 32 3840
Fragments arranged by size:
3840 2860 32 32
Tth111I GACn'n_nGTC
Cuts at: 2077 4561 2077
Size: 2484 4280
XbaI T'CTAG_A
Cuts at: 714 2299 3795 714
Size: 1585 1496 3683
Fragments arranged by size:
3683 1585 1496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4259 4259
Size: 6764
XhoI C'TCGA_G
Cuts at: 1445 2585 1445
Size: 1140 5624
XmaI C'CCGG_G
Cuts at: 3784 3784
Size: 6764
XmnI GAAnn'nnTTC
Cuts at: 2362 3191 2362
Size: 829 5935
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BbvCI
BfrBI BglI BmgBI BmtI BsaXI BseRI BsgI BsiWI
BspEI BssHII BstZ17I Bsu36I BtgI CspCI EcoNI FseI
FspAI NcoI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI ZraI