Restriction map for
pF1KE1029
(Circular) (Six-base) MAPSORT of: pF1KE1029.seq Check: 6276 from: 1 to: 6932
pF1KE1029 6932 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 855 3153 855
Size: 2298 4634
AccI GT'mk_AC
Cuts at: 536 2753 3723 3970 536
Size: 2217 970 247 3498
Fragments arranged by size:
3498 2217 970 247
Acc65I G'GTAC_C
Cuts at: 3948 3948
Size: 6932
AclI AA'CG_TT
Cuts at: 6772 6772
Size: 6932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 782 1468 2063 4757 5189 5542 6204 490
Size: 292 686 595 2694 432 353 662 1218
Fragments arranged by size:
2694 1218 686 662 595 432 353 292
AfeI AGC'GCT
Cuts at: 3397 3397
Size: 6932
AflIII A'CryG_T
Cuts at: 4284 6084 6365 4284
Size: 1800 281 4851
Fragments arranged by size:
4851 1800 281
AgeI A'CCGG_T
Cuts at: 138 138
Size: 6932
AhdI GACnn_n'nnGTC
Cuts at: 132 132
Size: 6932
AleI CACnn'nnGTG
Cuts at: 961 2672 961
Size: 1711 5221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1145 1145
Size: 6932
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1398 1430 1398
Size: 32 6900
AlwNI CAG_nnn'CTG
Cuts at: 241 468 820 4237 5675 241
Size: 227 352 3417 1438 1498
Fragments arranged by size:
3417 1498 1438 352 227
ApaI G_GGCC'C
Cuts at: 335 335
Size: 6932
ApaLI G'TGCA_C
Cuts at: 5770 5770
Size: 6932
ApoI r'AATT_y
Cuts at: 266 565 1551 1636 2056 3369 3426 3621 3936
Size: 299 986 85 420 1313 57 195 315
Cuts at: 3936 6285 6541 266
Size: 2349 256 657
Fragments arranged by size:
2349 1313 986 657 420 315 299 256
195 85 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 6932
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6932
AvaI C'yCGr_G
Cuts at: 3952 3952
Size: 6932
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1847 1880 1847
Size: 33 6899
BamHI G'GATC_C
Cuts at: 164 3503 3957 164
Size: 3339 454 3139
Fragments arranged by size:
3339 3139 454
BanI G'GyrC_C
Cuts at: 3834 3948 4362 4610 4645 3834
Size: 114 414 248 35 6121
Fragments arranged by size:
6121 414 248 114 35
BanII G_rGCy'C
Cuts at: 319 335 1774 3693 3946 4976 319
Size: 16 1439 1919 253 1030 2275
Fragments arranged by size:
2275 1919 1439 1030 253 16
BbeI G_GCGC'C
Cuts at: 4366 4614 4366
Size: 248 6684
BbsI GAAGACnn'nnnn_
Cuts at: 1918 2459 3574 1918
Size: 541 1115 5276
Fragments arranged by size:
5276 1115 541
BbvCI CC'TCA_GC
Cuts at: 836 836
Size: 6932
BciVI GTATCCnnnnn_n'
Cuts at: 4830 5886 6932 4830
Size: 1056 1046 4830
Fragments arranged by size:
4830 1056 1046
BclI T'GATC_A
Cuts at: 1314 3644 4452 1314
Size: 2330 808 3794
Fragments arranged by size:
3794 2330 808
BfrBI ATG'CAT
Cuts at: 925 2556 925
Size: 1631 5301
BglI GCCn_nnn'nGGC
Cuts at: 1362 1376 2653 1362
Size: 14 1277 5641
Fragments arranged by size:
5641 1277 14
BglII A'GATC_T
Cuts at: 3152 4447 6484 3152
Size: 1295 2037 3600
Fragments arranged by size:
3600 2037 1295
BlpI GC'TnA_GC
Cuts at: 1601 3269 4044 1601
Size: 1668 775 4489
Fragments arranged by size:
4489 1668 775
Bme1580I G_kGCm'C
Cuts at: 335 2086 3776 3839 4557 4650 5774 335
Size: 1751 1690 63 718 93 1124 1493
Fragments arranged by size:
1751 1690 1493 1124 718 93 63
BmrI ACTGGGnnnn_n'
Cuts at: 798 4144 4225 4560 6704 798
Size: 3346 81 335 2144 1026
Fragments arranged by size:
3346 2144 1026 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 431 1608 2614 5299 431
Size: 1177 1006 2685 2064
Fragments arranged by size:
2685 2064 1177 1006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1142 1163 1400 1758 2098 3265 3622 3844
Size: 28 21 237 358 340 1167 357 222
Cuts at: 3844 1114
Size: 4202
Fragments arranged by size:
4202 1167 358 357 340 237 222 28
21
Bpu10I CC'TnA_GC
Cuts at: 836 3446 836
Size: 2610 4322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3216 4108 4987 5701 5999 3216
Size: 892 879 714 298 4149
Fragments arranged by size:
4149 892 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 1972 3787 3856 6636 238
Size: 1734 1815 69 2780 534
Fragments arranged by size:
2780 1815 1734 534 69
BsaAI yAC'GTr
Cuts at: 3411 4285 3411
Size: 874 6058
BsaBI GATnn'nnATC
Cuts at: 6 4446 6483 6
Size: 4440 2037 455
Fragments arranged by size:
4440 2037 455
BsaHI Gr'CG_yC
Cuts at: 4363 4611 5313 6809 4363
Size: 248 702 1496 4486
Fragments arranged by size:
4486 1496 702 248
BsaWI w'CCGG_w
Cuts at: 138 3205 3506 4121 4642 5320 5731 5878 6152
Size: 3067 301 615 521 678 411 147 274
Cuts at: 6152 138
Size: 918
Fragments arranged by size:
3067 918 678 615 521 411 301 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 383 413 1313 1343 1398 1428 3238 3268 383
Size: 30 900 30 55 30 1810 30 4047
Fragments arranged by size:
4047 1810 900 55 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 824 1820 2419 3379 824
Size: 996 599 960 4377
Fragments arranged by size:
4377 996 960 599
BseYI C'CCAG_C
Cuts at: 152 286 614 791 813 824 1366 2898 2985
Size: 134 328 177 22 11 542 1532 87
Cuts at: 2985 4357 5115 5780 6188 152
Size: 1372 758 665 408 896
Fragments arranged by size:
1532 1372 896 758 665 542 408 328
177 134 87 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 419 463 1556 2785 3181 400
Size: 19 44 1093 1229 396 4151
Fragments arranged by size:
4151 1229 1093 396 44 19
BsiEI CG_ry'CG
Cuts at: 104 4474 4520 5750 104
Size: 4370 46 1230 1286
Fragments arranged by size:
4370 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 319 3946 4724 4914 5774 319
Size: 3627 778 190 860 1477
Fragments arranged by size:
3627 1477 860 778 190
BsmI GAATG_Cn'
Cuts at: 1087 2415 2516 1087
Size: 1328 101 5503
Fragments arranged by size:
5503 1328 101
BsmBI CGTCTCn'nnnn_
Cuts at: 1204 2950 1204
Size: 1746 5186
Bsp1286I G_dGCh'C
Cuts at: 319 335 1774 2086 3693 3776 3839 3946 4557
Size: 16 1439 312 1607 83 63 107 611
Cuts at: 4557 4650 4724 4914 4976 5774 319
Size: 93 74 190 62 798 1477
Fragments arranged by size:
1607 1477 1439 798 611 312 190 107
93 83 74 63 62 16
BspEI T'CCGG_A
Cuts at: 3506 3506
Size: 6932
BspHI T'CATG_A
Cuts at: 557 5364 557
Size: 4807 2125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 855 3153 3982 4196 4498 4879 855
Size: 2298 829 214 302 381 2908
Fragments arranged by size:
2908 2298 829 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5224 5278 6762 6927 50
Size: 5174 54 1484 165 55
Fragments arranged by size:
5174 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1730 2174 3083 4844 1730
Size: 444 909 1761 3818
Fragments arranged by size:
3818 1761 909 444
BsrFI r'CCGG_y
Cuts at: 138 942 4930 5111 138
Size: 804 3988 181 1959
Fragments arranged by size:
3988 1959 804 181
BsrGI T'GTAC_A
Cuts at: 1689 1816 1689
Size: 127 6805
BssSI C'ACGA_G
Cuts at: 1595 5203 5911 1595
Size: 3608 708 2616
Fragments arranged by size:
3608 2616 708
BstAPI GCAn_nnn'nTGC
Cuts at: 204 4309 204
Size: 4105 2827
BstBI TT'CG_AA
Cuts at: 5293 5293
Size: 6932
BstXI CCAn_nnnn'nTGG
Cuts at: 702 702
Size: 6932
BstYI r'GATC_y
Cuts at: 1 164 1385 3152 3503 3957 4424 4447 4782
Size: 163 1221 1767 351 454 467 23 335
Cuts at: 4782 5028 5334 5346 5432 6484 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1767 1221 1052 467 454 449 351 335
306 246 163 86 23 12
BstZ17I GTA'TAC
Cuts at: 2754 2754
Size: 6932
Bsu36I CC'TnA_GG
Cuts at: 530 2338 2818 530
Size: 1808 480 4644
Fragments arranged by size:
4644 1808 480
BtgI C'CryG_G
Cuts at: 387 747 3095 3572 387
Size: 360 2348 477 3747
Fragments arranged by size:
3747 2348 477 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4882 5063 5226 4882
Size: 181 163 6588
Fragments arranged by size:
6588 181 163
BtsI GCAGTG_nn'
Cuts at: 242 4175 242
Size: 3933 2999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1464 1499 1464
Size: 35 6897
DraI TTT'AAA
Cuts at: 570 1206 1662 1850 2316 3931 570
Size: 636 456 188 466 1615 3571
Fragments arranged by size:
3571 1615 636 466 456 188
DraIII CAC_nnn'GTG
Cuts at: 204 2499 204
Size: 2295 4637
DrdI GACnn_nn'nnGTC
Cuts at: 252 2332 4638 5982 252
Size: 2080 2306 1344 1202
Fragments arranged by size:
2306 2080 1344 1202
EaeI y'GGCC_r
Cuts at: 723 890 4517 4691 5082 5109 6454 723
Size: 167 3627 174 391 27 1345 1201
Fragments arranged by size:
3627 1345 1201 391 174 167 27
EagI C'GGCC_G
Cuts at: 4517 4517
Size: 6932
EarI CTCTTCn'nnn_
Cuts at: 1532 2497 2609 2621 2767 3356 4955 5165 1532
Size: 965 112 12 146 589 1599 210 3299
Fragments arranged by size:
3299 1599 965 589 210 146 112 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 929 3516 5140 5882 6028 6742 929
Size: 2587 1624 742 146 714 1119
Fragments arranged by size:
2587 1624 1119 742 714 146
EcoICRI GAG'CTC
Cuts at: 317 3944 317
Size: 3627 3305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 782 1114 1142 1163 1400 1468 1758 2063
Size: 292 332 28 21 237 68 290 305
Cuts at: 2063 2098 3265 3622 3844 4757 5189 5542 6204
Size: 35 1167 357 222 913 432 353 662
Cuts at: 6204 490
Size: 1218
Fragments arranged by size:
1218 1167 913 662 432 357 353 332
305 292 290 237 222 68 35 28
21
EcoO109I rG'GnC_Cy
Cuts at: 2334 3828 4071 2334
Size: 1494 243 5195
Fragments arranged by size:
5195 1494 243
EcoRI G'AATT_C
Cuts at: 266 3369 3426 3621 3936 266
Size: 3103 57 195 315 3262
Fragments arranged by size:
3262 3103 315 195 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4951 4951
Size: 6932
FspI TGC'GCA
Cuts at: 4713 4713
Size: 6932
HaeII r_GCGC'y
Cuts at: 3399 4366 4614 5844 6615 3399
Size: 967 248 1230 771 3716
Fragments arranged by size:
3716 1230 967 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 472 504 1210 1242 1299 1313 1331
Size: 32 322 32 706 32 57 14 18
Cuts at: 1331 1345 2151 2183 3385 3417 3424 3456 4774
Size: 14 806 32 1202 32 7 32 1318
Cuts at: 4774 4804 4806 4836 118
Size: 30 2 30 2214
Fragments arranged by size:
2214 1318 1202 806 706 322 57 32
32 32 32 32 32 30 30 18
14 14 7 2
HincII GTy'rAC
Cuts at: 2772 3251 3517 3971 2772
Size: 479 266 454 5733
Fragments arranged by size:
5733 479 454 266
HindIII A'AGCT_T
Cuts at: 2023 3301 2023
Size: 1278 5654
KasI G'GCGC_C
Cuts at: 4362 4610 4362
Size: 248 6684
KpnI G_GTAC'C
Cuts at: 3952 3952
Size: 6932
MfeI C'AATT_G
Cuts at: 2186 2186
Size: 6932
MluI A'CGCG_T
Cuts at: 6365 6365
Size: 6932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 142 5691 5875 142
Size: 5549 184 1199
Fragments arranged by size:
5549 1199 184
MscI TGG'CCA
Cuts at: 725 4693 725
Size: 3968 2964
MslI CAynn'nnrTG
Cuts at: 961 1484 1735 2672 3707 4921 5048 961
Size: 523 251 937 1035 1214 127 2845
Fragments arranged by size:
2845 1214 1035 937 523 251 127
MspA1I CmG'CkG
Cuts at: 376 433 2140 3129 3216 3261 3336 4043 4357
Size: 57 1707 989 87 45 75 707 314
Cuts at: 4357 4717 5501 5746 376
Size: 360 784 245 1562
Fragments arranged by size:
1707 1562 989 784 707 360 314 245
87 75 57 45
NaeI GCC'GGC
Cuts at: 5113 5113
Size: 6932
NarI GG'CG_CC
Cuts at: 4363 4611 4363
Size: 248 6684
NcoI C'CATG_G
Cuts at: 747 3095 3572 747
Size: 2348 477 4107
Fragments arranged by size:
4107 2348 477
NgoMIV G'CCGG_C
Cuts at: 5111 5111
Size: 6932
NsiI A_TGCA'T
Cuts at: 927 2558 927
Size: 1631 5301
NspI r_CATG'y
Cuts at: 3772 3985 4485 6088 3772
Size: 213 500 1603 4616
Fragments arranged by size:
4616 1603 500 213
PciI A'CATG_T
Cuts at: 6084 6084
Size: 6932
PflMI CCAn_nnn'nTGG
Cuts at: 3803 3803
Size: 6932
PfoI T'CCnGG_A
Cuts at: 1091 2993 3242 1091
Size: 1902 249 4781
Fragments arranged by size:
4781 1902 249
PmeI GTTT'AAAC
Cuts at: 3931 3931
Size: 6932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1398 1430 5378 5410 1398
Size: 32 3948 32 2920
Fragments arranged by size:
3948 2920 32 32
PpuMI rG'GwC_Cy
Cuts at: 2334 2334
Size: 6932
PspOMI G'GGCC_C
Cuts at: 331 331
Size: 6932
PstI C_TGCA'G
Cuts at: 515 3134 3979 4664 515
Size: 2619 845 685 2783
Fragments arranged by size:
2783 2619 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6932
PvuII CAG'CTG
Cuts at: 433 2140 3129 3216 3336 4357 4717 433
Size: 1707 989 87 120 1021 360 2648
Fragments arranged by size:
2648 1707 1021 989 360 120 87
RsrII CG'GwC_CG
Cuts at: 5127 5127
Size: 6932
SacI G_AGCT'C
Cuts at: 319 3946 319
Size: 3627 3305
SalI G'TCGA_C
Cuts at: 3969 3969
Size: 6932
SapI GCTCTTCn'nnn_
Cuts at: 1532 4955 5165 1532
Size: 3423 210 3299
Fragments arranged by size:
3423 3299 210
SbfI CC_TGCA'GG
Cuts at: 3979 3979
Size: 6932
ScaI AGT'ACT
Cuts at: 274 6449 274
Size: 6175 757
SexAI A'CCwGG_T
Cuts at: 293 500 293
Size: 207 6725
SfcI C'TryA_G
Cuts at: 33 338 511 1400 3130 3975 4660 5628 5819
Size: 305 173 889 1730 845 685 968 191
Cuts at: 5819 33
Size: 1146
Fragments arranged by size:
1730 1146 968 889 845 685 305 191
173
SfoI GGC'GCC
Cuts at: 4364 4612 4364
Size: 248 6684
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6932
SgrAI Cr'CCGG_yG
Cuts at: 138 138
Size: 6932
SmaI CCC'GGG
Cuts at: 3954 3954
Size: 6932
SmlI C'TyrA_G
Cuts at: 3231 4087 5002 5716 5978 3231
Size: 856 915 714 262 4185
Fragments arranged by size:
4185 915 856 714 262
SpeI A'CTAG_T
Cuts at: 67 1614 67
Size: 1547 5385
SphI G_CATG'C
Cuts at: 3985 4485 3985
Size: 500 6432
StuI AGG'CCT
Cuts at: 3742 3742
Size: 6932
StyI C'CwwG_G
Cuts at: 747 1133 2076 2330 3095 3321 3572 3761 4066
Size: 386 943 254 765 226 251 189 305
Cuts at: 4066 4420 747
Size: 354 3259
Fragments arranged by size:
3259 943 765 386 354 305 254 251
226 189
SwaI ATTT'AAAT
Cuts at: 570 570
Size: 6932
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 383 760 3802 4515 4648 5107 5316 300
Size: 83 377 3042 713 133 459 209 1916
Fragments arranged by size:
3042 1916 713 459 377 209 133 83
TatI w'GTAC_w
Cuts at: 272 1689 1816 6447 272
Size: 1417 127 4631 757
Fragments arranged by size:
4631 1417 757 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 383 415 6449 6481 383
Size: 32 6034 32 834
Fragments arranged by size:
6034 834 32 32
Tth111I GACn'n_nGTC
Cuts at: 4729 4729
Size: 6932
XbaI T'CTAG_A
Cuts at: 3963 3963
Size: 6932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2061 3579 4427 2061
Size: 1518 848 4566
Fragments arranged by size:
4566 1518 848
XmaI C'CCGG_G
Cuts at: 3952 3952
Size: 6932
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AscI AvrII BcgI BmgBI BmtI BsiWI
BssHII BstEII ClaI EcoNI EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI XhoI XmnI ZraI