Restriction map for
pF1KE1030
(Circular) (Six-base) MAPSORT of: pF1KE1030.seq Check: 5307 from: 1 to: 7013
pF1KE1030 7013 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1022 1389 4051 1022
Size: 367 2662 3984
Fragments arranged by size:
3984 2662 367
Acc65I G'GTAC_C
Cuts at: 663 4029 663
Size: 3366 3647
AclI AA'CG_TT
Cuts at: 6853 6853
Size: 7013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 940 968 1019 1886 1985 2075 2686 3332
Size: 344 28 51 867 99 90 611 646
Cuts at: 3332 3557 3937 4838 5270 5623 6285 596
Size: 225 380 901 432 353 662 1324
Fragments arranged by size:
1324 901 867 662 646 611 432 380
353 344 225 99 90 51 28
AflII C'TTAA_G
Cuts at: 2017 2503 2017
Size: 486 6527
AflIII A'CryG_T
Cuts at: 1290 2835 4365 6165 6446 1290
Size: 1545 1530 1800 281 1857
Fragments arranged by size:
1857 1800 1545 1530 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2111 2111
Size: 7013
AlwNI CAG_nnn'CTG
Cuts at: 1701 2076 3804 4318 5756 1701
Size: 375 1728 514 1438 2958
Fragments arranged by size:
2958 1728 1438 514 375
ApaLI G'TGCA_C
Cuts at: 5851 5851
Size: 7013
ApoI r'AATT_y
Cuts at: 1669 1859 2037 2183 2203 2421 3464 4017 6366
Size: 190 178 146 20 218 1043 553 2349
Cuts at: 6366 6622 1669
Size: 256 2060
Fragments arranged by size:
2349 2060 1043 553 256 218 190 178
146 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 7013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
AvaI C'yCGr_G
Cuts at: 350 4033 350
Size: 3683 3330
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1712 1745 1712
Size: 33 6980
BamHI G'GATC_C
Cuts at: 893 4038 893
Size: 3145 3868
BanI G'GyrC_C
Cuts at: 663 4029 4443 4691 4726 663
Size: 3366 414 248 35 2950
Fragments arranged by size:
3366 2950 414 248 35
BanII G_rGCy'C
Cuts at: 4027 5057 4027
Size: 1030 5983
BbeI G_GCGC'C
Cuts at: 4447 4695 4447
Size: 248 6765
BbsI GAAGACnn'nnnn_
Cuts at: 762 928 988 3881 762
Size: 166 60 2893 3894
Fragments arranged by size:
3894 2893 166 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 569 603 569
Size: 34 6979
BciVI GTATCCnnnnn_n'
Cuts at: 4911 5967 7013 4911
Size: 1056 1046 4911
Fragments arranged by size:
4911 1056 1046
BclI T'GATC_A
Cuts at: 390 2469 4533 390
Size: 2079 2064 2870
Fragments arranged by size:
2870 2079 2064
BfrBI ATG'CAT
Cuts at: 2315 2315
Size: 7013
BglII A'GATC_T
Cuts at: 4528 6565 4528
Size: 2037 4976
BlpI GC'TnA_GC
Cuts at: 1544 2459 4125 1544
Size: 915 1666 4432
Fragments arranged by size:
4432 1666 915
Bme1580I G_kGCm'C
Cuts at: 4638 4731 5855 4638
Size: 93 1124 5796
Fragments arranged by size:
5796 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 541 4225 4306 4641 6785 541
Size: 3684 81 335 2144 769
Fragments arranged by size:
3684 2144 769 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 365 5380 365
Size: 5015 1998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 3826 3860 2578
Size: 1248 34 5731
Fragments arranged by size:
5731 1248 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 2920 4189 5068 5782 6080 816
Size: 2104 1269 879 714 298 1749
Fragments arranged by size:
2104 1749 1269 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3142 6717 3142
Size: 3575 3438
BsaAI yAC'GTr
Cuts at: 4366 4366
Size: 7013
BsaBI GATnn'nnATC
Cuts at: 6 4527 6564 6
Size: 4521 2037 455
Fragments arranged by size:
4521 2037 455
BsaHI Gr'CG_yC
Cuts at: 4444 4692 5394 6890 4444
Size: 248 702 1496 4567
Fragments arranged by size:
4567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4202 4723 5401 5812 5959 6233 4202
Size: 521 678 411 147 274 4982
Fragments arranged by size:
4982 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3892 3922 3892
Size: 30 6983
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2410 3373 2410
Size: 963 6050
BseYI C'CCAG_C
Cuts at: 4438 5196 5861 6269 4438
Size: 758 665 408 5182
Fragments arranged by size:
5182 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 2084 2080
Size: 4 7009
BsiEI CG_ry'CG
Cuts at: 104 4555 4601 5831 104
Size: 4451 46 1230 1286
Fragments arranged by size:
4451 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3380 3992 4027 4805 4995 5855 3380
Size: 612 35 778 190 860 4538
Fragments arranged by size:
4538 860 778 612 190 35
BsmI GAATG_Cn'
Cuts at: 1545 2315 2409 1545
Size: 770 94 6149
Fragments arranged by size:
6149 770 94
Bsp1286I G_dGCh'C
Cuts at: 3380 3992 4027 4638 4731 4805 4995 5057 5855
Size: 612 35 611 93 74 190 62 798
Cuts at: 5855 3380
Size: 4538
Fragments arranged by size:
4538 798 612 611 190 93 74 62
35
BspHI T'CATG_A
Cuts at: 191 3087 5445 191
Size: 2896 2358 1759
Fragments arranged by size:
2896 2358 1759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 480 1806 4063 4277 4579 4960 480
Size: 1326 2257 214 302 381 2533
Fragments arranged by size:
2533 2257 1326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5305 5359 6843 7008 50
Size: 5255 54 1484 165 55
Fragments arranged by size:
5255 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 685 1221 4925 685
Size: 536 3704 2773
Fragments arranged by size:
3704 2773 536
BsrFI r'CCGG_y
Cuts at: 5011 5192 5011
Size: 181 6832
BsrGI T'GTAC_A
Cuts at: 3003 3003
Size: 7013
BssSI C'ACGA_G
Cuts at: 5284 5992 5284
Size: 708 6305
BstAPI GCAn_nnn'nTGC
Cuts at: 4390 4390
Size: 7013
BstBI TT'CG_AA
Cuts at: 5374 5374
Size: 7013
BstXI CCAn_nnnn'nTGG
Cuts at: 904 904
Size: 7013
BstYI r'GATC_y
Cuts at: 1 893 2802 4038 4505 4528 4863 5109 5415
Size: 892 1909 1236 467 23 335 246 306
Cuts at: 5415 5427 5513 6565 1
Size: 12 86 1052 449
Fragments arranged by size:
1909 1236 1052 892 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1390 1390
Size: 7013
BtgI C'CryG_G
Cuts at: 107 107
Size: 7013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 644 4963 5144 5307 644
Size: 4319 181 163 2350
Fragments arranged by size:
4319 2350 181 163
BtsI GCAGTG_nn'
Cuts at: 4256 4256
Size: 7013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 821 856 821
Size: 35 6978
DraI TTT'AAA
Cuts at: 360 960 1064 2828 4012 360
Size: 600 104 1764 1184 3361
Fragments arranged by size:
3361 1764 1184 600 104
DrdI GACnn_nn'nnGTC
Cuts at: 4719 6063 4719
Size: 1344 5669
EaeI y'GGCC_r
Cuts at: 1676 4598 4772 5163 5190 6535 1676
Size: 2922 174 391 27 1345 2154
Fragments arranged by size:
2922 2154 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4598 4598
Size: 7013
EarI CTCTTCn'nnn_
Cuts at: 181 479 1618 1793 2003 2159 2387 2543 2753
Size: 298 1139 175 210 156 228 156 210
Cuts at: 2753 2880 2929 3624 5036 5246 181
Size: 127 49 695 1412 210 1948
Fragments arranged by size:
1948 1412 1139 695 298 228 210 210
210 175 156 156 127 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5221 5963 6109 6823 5221
Size: 742 146 714 5411
Fragments arranged by size:
5411 742 714 146
EcoICRI GAG'CTC
Cuts at: 4025 4025
Size: 7013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 940 968 1019 1886 1985 2075 2578 2686
Size: 344 28 51 867 99 90 503 108
Cuts at: 2686 3332 3557 3826 3860 3937 4838 5270 5623
Size: 646 225 269 34 77 901 432 353
Cuts at: 5623 6285 596
Size: 662 1324
Fragments arranged by size:
1324 901 867 662 646 503 432 353
344 269 225 108 99 90 77 51
34 28
EcoO109I rG'GnC_Cy
Cuts at: 2073 4152 2073
Size: 2079 4934
EcoRI G'AATT_C
Cuts at: 4017 4017
Size: 7013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 985 2032 2374 5032 985
Size: 1047 342 2658 2966
Fragments arranged by size:
2966 2658 1047 342
FspI TGC'GCA
Cuts at: 4794 4794
Size: 7013
HaeII r_GCGC'y
Cuts at: 4447 4695 5925 6696 4447
Size: 248 1230 771 4764
Fragments arranged by size:
4764 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1224 1256 1388 1420 3892 3924 4855 4885 4887
Size: 32 132 32 2472 32 931 30 2
Cuts at: 4887 4917 1224
Size: 30 3320
Fragments arranged by size:
3320 2472 931 132 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1181 4052 1181
Size: 2871 4142
HindIII A'AGCT_T
Cuts at: 244 737 1711 244
Size: 493 974 5546
Fragments arranged by size:
5546 974 493
HpaI GTT'AAC
Cuts at: 1181 1181
Size: 7013
KasI G'GCGC_C
Cuts at: 4443 4691 4443
Size: 248 6765
KpnI G_GTAC'C
Cuts at: 667 4033 667
Size: 3366 3647
MluI A'CGCG_T
Cuts at: 6446 6446
Size: 7013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 1724 3209 5772 5956 356
Size: 1368 1485 2563 184 1413
Fragments arranged by size:
2563 1485 1413 1368 184
MscI TGG'CCA
Cuts at: 1678 4774 1678
Size: 3096 3917
MslI CAynn'nnrTG
Cuts at: 5002 5129 5002
Size: 127 6886
MspA1I CmG'CkG
Cuts at: 583 1633 2991 4124 4438 4798 5582 5827 583
Size: 1050 1358 1133 314 360 784 245 1769
Fragments arranged by size:
1769 1358 1133 1050 784 360 314 245
NaeI GCC'GGC
Cuts at: 5194 5194
Size: 7013
NarI GG'CG_CC
Cuts at: 4444 4692 4444
Size: 248 6765
NcoI C'CATG_G
Cuts at: 107 107
Size: 7013
NgoMIV G'CCGG_C
Cuts at: 5192 5192
Size: 7013
NsiI A_TGCA'T
Cuts at: 2317 2317
Size: 7013
NspI r_CATG'y
Cuts at: 1294 2839 4066 4566 6169 1294
Size: 1545 1227 500 1603 2138
Fragments arranged by size:
2138 1603 1545 1227 500
PciI A'CATG_T
Cuts at: 1290 2835 6165 1290
Size: 1545 3330 2138
Fragments arranged by size:
3330 2138 1545
PmeI GTTT'AAAC
Cuts at: 4012 4012
Size: 7013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5459 5491 5459
Size: 32 6981
PshAI GACnn'nnGTC
Cuts at: 833 833
Size: 7013
PsiI TTA'TAA
Cuts at: 1225 1225
Size: 7013
PstI C_TGCA'G
Cuts at: 1446 4060 4745 1446
Size: 2614 685 3714
Fragments arranged by size:
3714 2614 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7013
PvuII CAG'CTG
Cuts at: 583 2991 4438 4798 583
Size: 2408 1447 360 2798
Fragments arranged by size:
2798 2408 1447 360
RsrII CG'GwC_CG
Cuts at: 5208 5208
Size: 7013
SacI G_AGCT'C
Cuts at: 4027 4027
Size: 7013
SalI G'TCGA_C
Cuts at: 4050 4050
Size: 7013
SapI GCTCTTCn'nnn_
Cuts at: 479 2159 2543 5036 5246 479
Size: 1680 384 2493 210 2246
Fragments arranged by size:
2493 2246 1680 384 210
SbfI CC_TGCA'GG
Cuts at: 4060 4060
Size: 7013
ScaI AGT'ACT
Cuts at: 6530 6530
Size: 7013
SexAI A'CCwGG_T
Cuts at: 3995 3995
Size: 7013
SfcI C'TryA_G
Cuts at: 33 1442 1513 2257 2543 3478 4056 4741 5709
Size: 1409 71 744 286 935 578 685 968
Cuts at: 5709 5900 33
Size: 191 1146
Fragments arranged by size:
1409 1146 968 935 744 685 578 286
191 71
SfoI GGC'GCC
Cuts at: 4445 4693 4445
Size: 248 6765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7013
SmaI CCC'GGG
Cuts at: 4035 4035
Size: 7013
SmlI C'TyrA_G
Cuts at: 350 831 2017 2503 2899 4168 5083 5797 6059
Size: 481 1186 486 396 1269 915 714 262
Cuts at: 6059 350
Size: 1304
Fragments arranged by size:
1304 1269 1186 915 714 486 481 396
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7013
SphI G_CATG'C
Cuts at: 4066 4566 4066
Size: 500 6513
StuI AGG'CCT
Cuts at: 2872 2872
Size: 7013
StyI C'CwwG_G
Cuts at: 107 1724 4147 4501 107
Size: 1617 2423 354 2619
Fragments arranged by size:
2619 2423 1617 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 940 4596 4729 5188 5397 940
Size: 3656 133 459 209 2556
Fragments arranged by size:
3656 2556 459 209 133
TatI w'GTAC_w
Cuts at: 1692 3003 6528 1692
Size: 1311 3525 2177
Fragments arranged by size:
3525 2177 1311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6530 6562 6530
Size: 32 6981
Tth111I GACn'n_nGTC
Cuts at: 3906 4810 3906
Size: 904 6109
XbaI T'CTAG_A
Cuts at: 3372 4044 3372
Size: 672 6341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2524 4508 2524
Size: 1984 5029
XhoI C'TCGA_G
Cuts at: 350 350
Size: 7013
XmaI C'CCGG_G
Cuts at: 4033 4033
Size: 7013
XmnI GAAnn'nnTTC
Cuts at: 1604 2552 1604
Size: 948 6065
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BbvCI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BspEI BssHII BstEII Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI