Restriction map for
pF1KE1031
(Circular) (Six-base) MAPSORT of: pF1KE1031.seq Check: 881 from: 1 to: 7292
pF1KE1031 7292 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2205 2975 2205
Size: 770 6522
AatII G_ACGT'C
Cuts at: 3724 3724
Size: 7292
AccI GT'mk_AC
Cuts at: 4330 4330
Size: 7292
Acc65I G'GTAC_C
Cuts at: 1562 4308 1562
Size: 2746 4546
AclI AA'CG_TT
Cuts at: 7132 7132
Size: 7292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 1174 1688 3431 3943 3959 5117 5549 5902
Size: 560 514 1743 512 16 1158 432 353
Cuts at: 5902 6564 614
Size: 662 1342
Fragments arranged by size:
1743 1342 1158 662 560 514 512 432
353 16
AfeI AGC'GCT
Cuts at: 3008 3008
Size: 7292
AflII C'TTAA_G
Cuts at: 1410 1410
Size: 7292
AflIII A'CryG_T
Cuts at: 3861 4644 6444 6725 3861
Size: 783 1800 281 4428
Fragments arranged by size:
4428 1800 783 281
AhdI GACnn_n'nnGTC
Cuts at: 1708 3192 1708
Size: 1484 5808
AleI CACnn'nnGTG
Cuts at: 4005 4005
Size: 7292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 431 3511 4123 431
Size: 3080 612 3600
Fragments arranged by size:
3600 3080 612
AlwNI CAG_nnn'CTG
Cuts at: 937 1290 1783 2976 3018 3333 3639 4248 4597
Size: 353 493 1193 42 315 306 609 349
Cuts at: 4597 6035 937
Size: 1438 2194
Fragments arranged by size:
2194 1438 1193 609 493 353 349 315
306 42
ApaI G_GGCC'C
Cuts at: 607 640 1371 2485 2949 3798 607
Size: 33 731 1114 464 849 4101
Fragments arranged by size:
4101 1114 849 731 464 33
ApaLI G'TGCA_C
Cuts at: 6130 6130
Size: 7292
ApoI r'AATT_y
Cuts at: 4296 6645 6901 4296
Size: 2349 256 4687
Fragments arranged by size:
4687 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7292
AvaI C'yCGr_G
Cuts at: 2733 2871 3305 3459 4312 2733
Size: 138 434 154 853 5713
Fragments arranged by size:
5713 853 434 154 138
AvrII C'CTAG_G
Cuts at: 676 676
Size: 7292
BamHI G'GATC_C
Cuts at: 4317 4317
Size: 7292
BanI G'GyrC_C
Cuts at: 649 1562 2220 3533 4007 4308 4722 4970 5005
Size: 913 658 1313 474 301 414 248 35
Cuts at: 5005 649
Size: 2936
Fragments arranged by size:
2936 1313 913 658 474 414 301 248
35
BanII G_rGCy'C
Cuts at: 607 640 752 1016 1371 1512 1541 1724 2189
Size: 33 112 264 355 141 29 183 465
Cuts at: 2189 2234 2408 2485 2949 3798 3882 4078 4205
Size: 45 174 77 464 849 84 196 127
Cuts at: 4205 4306 5336 607
Size: 101 1030 2563
Fragments arranged by size:
2563 1030 849 465 464 355 264 196
183 174 141 127 112 101 84 77
45 33 29
BbeI G_GCGC'C
Cuts at: 3537 4726 4974 3537
Size: 1189 248 5855
Fragments arranged by size:
5855 1189 248
BbsI GAAGACnn'nnnn_
Cuts at: 1180 3947 1180
Size: 2767 4525
BbvCI CC'TCA_GC
Cuts at: 391 1995 2069 2351 391
Size: 1604 74 282 5332
Fragments arranged by size:
5332 1604 282 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7258
BciVI GTATCCnnnnn_n'
Cuts at: 5190 6246 7292 5190
Size: 1056 1046 5190
Fragments arranged by size:
5190 1056 1046
BclI T'GATC_A
Cuts at: 402 4812 402
Size: 4410 2882
BglI GCCn_nnn'nGGC
Cuts at: 2863 3328 3574 2863
Size: 465 246 6581
Fragments arranged by size:
6581 465 246
BglII A'GATC_T
Cuts at: 895 4807 6844 895
Size: 3912 2037 1343
Fragments arranged by size:
3912 2037 1343
BlpI GC'TnA_GC
Cuts at: 767 2116 2233 2766 3067 3115 4404 767
Size: 1349 117 533 301 48 1289 3655
Fragments arranged by size:
3655 1349 1289 533 301 117 48
Bme1580I G_kGCm'C
Cuts at: 479 607 629 640 1371 1867 2485 2492 2949
Size: 128 22 11 731 496 618 7 457
Cuts at: 2949 3490 3560 3798 4012 4917 5010 6134 479
Size: 541 70 238 214 905 93 1124 1637
Fragments arranged by size:
1637 1124 905 731 618 541 496 457
238 214 128 93 70 22 11 7
BmgBI CAC'GTC
Cuts at: 1215 1215
Size: 7292
BmrI ACTGGGnnnn_n'
Cuts at: 473 2188 2479 2638 2734 3622 3894 4504 4585
Size: 1715 291 159 96 888 272 610 81
Cuts at: 4585 4920 7064 473
Size: 335 2144 701
Fragments arranged by size:
2144 1715 888 701 610 335 291 272
159 96 81
BmtI G_CTAG'C
Cuts at: 1399 1399
Size: 7292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 951 3786 3908 5659 951
Size: 2835 122 1751 2584
Fragments arranged by size:
2835 2584 1751 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1054 1129 1547 1783 1879 2392 2553 2554
Size: 813 75 418 236 96 513 161 1
Cuts at: 2554 2863 2878 3154 3937 4225 241
Size: 309 15 276 783 288 3308
Fragments arranged by size:
3308 813 783 513 418 309 288 276
236 161 96 75 15 1
Bpu10I CC'TnA_GC
Cuts at: 391 751 1995 2069 2351 3245 391
Size: 360 1244 74 282 894 4438
Fragments arranged by size:
4438 1244 894 360 282 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 3659 4468 5347 6061 6359 912
Size: 2747 809 879 714 298 1845
Fragments arranged by size:
2747 1845 879 809 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 111 1919 4147 4276 6996 111
Size: 1808 2228 129 2720 407
Fragments arranged by size:
2720 2228 1808 407 129
BsaAI yAC'GTr
Cuts at: 1758 3864 4645 1758
Size: 2106 781 4405
Fragments arranged by size:
4405 2106 781
BsaBI GATnn'nnATC
Cuts at: 6 4806 6843 6
Size: 4800 2037 455
Fragments arranged by size:
4800 2037 455
BsaHI Gr'CG_yC
Cuts at: 3534 3721 4723 4971 5673 7169 3534
Size: 187 1002 248 702 1496 3657
Fragments arranged by size:
3657 1496 1002 702 248 187
BsaWI w'CCGG_w
Cuts at: 2042 3971 4481 5002 5680 6091 6238 6512 2042
Size: 1929 510 521 678 411 147 274 2822
Fragments arranged by size:
2822 1929 678 521 510 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 657 687 2572 2602 3918 3948 657
Size: 30 1885 30 1316 30 4001
Fragments arranged by size:
4001 1885 1316 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 314 408 844 955 1270 1531 1549 2281 2608
Size: 94 436 111 315 261 18 732 327
Cuts at: 2608 2788 3643 314
Size: 180 855 3963
Fragments arranged by size:
3963 855 732 436 327 315 261 180
111 94 18
BseYI C'CCAG_C
Cuts at: 189 306 343 863 874 1047 1319 1594 1638
Size: 117 37 520 11 173 272 275 44
Cuts at: 1638 1999 2297 2710 3265 3659 4717 5475 6140
Size: 361 298 413 555 394 1058 758 665
Cuts at: 6140 6548 189
Size: 408 933
Fragments arranged by size:
1058 933 758 665 555 520 413 408
394 361 298 275 272 173 117 44
37 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 203 217 1072 1225 3319 4144 136
Size: 67 14 855 153 2094 825 3284
Fragments arranged by size:
3284 2094 855 825 153 67 14
BsiEI CG_ry'CG
Cuts at: 104 4834 4880 6110 104
Size: 4730 46 1230 1286
Fragments arranged by size:
4730 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1332 1503 1541 1814 2122 2189 2234 3882 4019
Size: 171 38 273 308 67 45 1648 137
Cuts at: 4019 4078 4205 4265 4306 5084 5274 6134 1332
Size: 59 127 60 41 778 190 860 2490
Fragments arranged by size:
2490 1648 860 778 308 273 190 171
137 127 67 60 59 45 41 38
BsmI GAATG_Cn'
Cuts at: 1639 1830 1639
Size: 191 7101
BsmBI CGTCTCn'nnnn_
Cuts at: 2755 2755
Size: 7292
Bsp1286I G_dGCh'C
Cuts at: 479 607 629 640 752 1016 1332 1371 1503
Size: 128 22 11 112 264 316 39 132
Cuts at: 1503 1512 1541 1724 1814 1867 2122 2189 2234
Size: 9 29 183 90 53 255 67 45
Cuts at: 2234 2408 2485 2492 2949 3490 3560 3798 3882
Size: 174 77 7 457 541 70 238 84
Cuts at: 3882 4012 4019 4078 4205 4265 4306 4917 5010
Size: 130 7 59 127 60 41 611 93
Cuts at: 5010 5084 5274 5336 6134 479
Size: 74 190 62 798 1637
Fragments arranged by size:
1637 798 611 541 457 316 264 255
238 190 183 174 132 130 128 127
112 93 90 84 77 74 70 67
62 60 59 53 45 41 39 29
22 11 9 7 7
BspEI T'CCGG_A
Cuts at: 2042 3971 2042
Size: 1929 5363
BspHI T'CATG_A
Cuts at: 5724 5724
Size: 7292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 1229 1791 2205 2975 4342 4556 4858 5239
Size: 1074 562 414 770 1367 214 302 381
Cuts at: 5239 155
Size: 2208
Fragments arranged by size:
2208 1367 1074 770 562 414 381 302
214
BsrBI CCG'CTC
Cuts at: 50 255 1270 5584 5638 7122 7287 50
Size: 205 1015 4314 54 1484 165 55
Fragments arranged by size:
4314 1484 1015 205 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1057 1481 3291 5204 1057
Size: 424 1810 1913 3145
Fragments arranged by size:
3145 1913 1810 424
BsrFI r'CCGG_y
Cuts at: 245 1391 1679 2314 3071 5290 5471 245
Size: 1146 288 635 757 2219 181 2066
Fragments arranged by size:
2219 2066 1146 757 635 288 181
BssSI C'ACGA_G
Cuts at: 2716 5563 6271 2716
Size: 2847 708 3737
Fragments arranged by size:
3737 2847 708
BstAPI GCAn_nnn'nTGC
Cuts at: 181 855 2220 3018 3513 4158 4669 181
Size: 674 1365 798 495 645 511 2804
Fragments arranged by size:
2804 1365 798 674 645 511 495
BstBI TT'CG_AA
Cuts at: 5653 5653
Size: 7292
BstEII G'GTnAC_C
Cuts at: 2170 2170
Size: 7292
BstXI CCAn_nnnn'nTGG
Cuts at: 700 1233 3318 3750 3790 700
Size: 533 2085 432 40 4202
Fragments arranged by size:
4202 2085 533 432 40
BstYI r'GATC_y
Cuts at: 1 833 895 2825 4317 4784 4807 5142 5388
Size: 832 62 1930 1492 467 23 335 246
Cuts at: 5388 5694 5706 5792 6844 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1930 1492 1052 832 467 449 335 306
246 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 365 827 2650 365
Size: 462 1823 5007
Fragments arranged by size:
5007 1823 462
BtgI C'CryG_G
Cuts at: 818 1248 1566 2224 2477 3482 818
Size: 430 318 658 253 1005 4628
Fragments arranged by size:
4628 1005 658 430 318 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5242 5423 5586 5242
Size: 181 163 6948
Fragments arranged by size:
6948 181 163
BtsI GCAGTG_nn'
Cuts at: 200 354 413 1336 1410 2278 2608 2655 2789
Size: 154 59 923 74 868 330 47 134
Cuts at: 2789 3016 3514 4535 200
Size: 227 498 1021 2957
Fragments arranged by size:
2957 1021 923 868 498 330 227 154
134 74 59 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 437 472 437
Size: 35 7257
DraI TTT'AAA
Cuts at: 4291 4291
Size: 7292
DrdI GACnn_nn'nnGTC
Cuts at: 2397 4998 6342 2397
Size: 2601 1344 3347
Fragments arranged by size:
3347 2601 1344
EaeI y'GGCC_r
Cuts at: 351 458 2160 2366 2844 4877 5051 5442 5469
Size: 107 1702 206 478 2033 174 391 27
Cuts at: 5469 6814 351
Size: 1345 829
Fragments arranged by size:
2033 1702 1345 829 478 391 206 174
107 27
EagI C'GGCC_G
Cuts at: 4877 4877
Size: 7292
EarI CTCTTCn'nnn_
Cuts at: 377 788 1015 1444 1508 2810 5315 5525 377
Size: 411 227 429 64 1302 2505 210 2144
Fragments arranged by size:
2505 2144 1302 429 411 227 210 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1807 3985 5500 6242 6388 7102 1807
Size: 2178 1515 742 146 714 1997
Fragments arranged by size:
2178 1997 1515 742 714 146
EcoICRI GAG'CTC
Cuts at: 1539 2187 2232 3880 4076 4203 4304 1539
Size: 648 45 1648 196 127 101 4527
Fragments arranged by size:
4527 1648 648 196 127 101 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 614 1054 1129 1174 1547 1688 1783 1879
Size: 373 440 75 45 373 141 95 96
Cuts at: 1879 2392 2553 2554 2863 2878 3154 3431 3937
Size: 513 161 1 309 15 276 277 506
Cuts at: 3937 3943 3959 4225 5117 5549 5902 6564 241
Size: 6 16 266 892 432 353 662 969
Fragments arranged by size:
969 892 662 513 506 440 432 373
373 353 309 277 276 266 161 141
96 95 75 45 16 15 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 364 2590 364
Size: 2226 5066
EcoO109I rG'GnC_Cy
Cuts at: 375 603 724 958 1367 1780 1854 1930 2127
Size: 228 121 234 409 413 74 76 197
Cuts at: 2127 2946 3258 3501 3794 3795 4065 4431 375
Size: 819 312 243 293 1 270 366 3236
Fragments arranged by size:
3236 819 413 409 366 312 293 270
243 234 228 197 121 76 74 1
EcoRI G'AATT_C
Cuts at: 4296 4296
Size: 7292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5311 5311
Size: 7292
FspI TGC'GCA
Cuts at: 5073 5073
Size: 7292
HaeII r_GCGC'y
Cuts at: 2586 3010 3537 4726 4974 6204 6975 2586
Size: 424 527 1189 248 1230 771 2903
Fragments arranged by size:
2903 1230 1189 771 527 424 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 387 419 657 689 1161 1193 1569
Size: 32 132 32 238 32 472 32 376
Cuts at: 1569 1601 1694 1726 2572 2604 3178 3210 3442
Size: 32 93 32 846 32 574 32 232
Cuts at: 3442 3474 5134 5164 5166 5196 223
Size: 32 1660 30 2 30 2319
Fragments arranged by size:
2319 1660 846 574 472 376 238 232
132 93 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4331 4331
Size: 7292
HindIII A'AGCT_T
Cuts at: 4168 4168
Size: 7292
KasI G'GCGC_C
Cuts at: 3533 4722 4970 3533
Size: 1189 248 5855
Fragments arranged by size:
5855 1189 248
KpnI G_GTAC'C
Cuts at: 1566 4312 1566
Size: 2746 4546
MluI A'CGCG_T
Cuts at: 6725 6725
Size: 7292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 6051 6235 263
Size: 5788 184 1320
Fragments arranged by size:
5788 1320 184
MscI TGG'CCA
Cuts at: 353 2162 2846 5053 353
Size: 1809 684 2207 2592
Fragments arranged by size:
2592 2207 1809 684
MslI CAynn'nnrTG
Cuts at: 269 1247 3493 3589 4005 5281 5408 269
Size: 978 2246 96 416 1276 127 2153
Fragments arranged by size:
2246 2153 1276 978 416 127 96
MspA1I CmG'CkG
Cuts at: 178 201 852 1999 2226 2268 2679 2850 3192
Size: 23 651 1147 227 42 411 171 342
Cuts at: 3192 3393 4403 4717 5077 5861 6106 178
Size: 201 1010 314 360 784 245 1364
Fragments arranged by size:
1364 1147 1010 784 651 411 360 342
314 245 227 201 171 42 23
NaeI GCC'GGC
Cuts at: 1681 2316 3073 5473 1681
Size: 635 757 2400 3500
Fragments arranged by size:
3500 2400 757 635
NarI GG'CG_CC
Cuts at: 3534 4723 4971 3534
Size: 1189 248 5855
Fragments arranged by size:
5855 1189 248
NcoI C'CATG_G
Cuts at: 818 1248 1566 3482 818
Size: 430 318 1916 4628
Fragments arranged by size:
4628 1916 430 318
NgoMIV G'CCGG_C
Cuts at: 1679 2314 3071 5471 1679
Size: 635 757 2400 3500
Fragments arranged by size:
3500 2400 757 635
NheI G'CTAG_C
Cuts at: 1395 1395
Size: 7292
NspI r_CATG'y
Cuts at: 1345 4345 4845 6448 1345
Size: 3000 500 1603 2189
Fragments arranged by size:
3000 2189 1603 500
PciI A'CATG_T
Cuts at: 6444 6444
Size: 7292
PflMI CCAn_nnn'nTGG
Cuts at: 613 1910 2085 2577 2901 3407 613
Size: 1297 175 492 324 506 4498
Fragments arranged by size:
4498 1297 506 492 324 175
PfoI T'CCnGG_A
Cuts at: 257 1524 1856 2570 3143 3617 3749 257
Size: 1267 332 714 573 474 132 3800
Fragments arranged by size:
3800 1267 714 573 474 332 132
PmeI GTTT'AAAC
Cuts at: 4291 4291
Size: 7292
PmlI CAC'GTG
Cuts at: 3864 3864
Size: 7292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5738 5770 5738
Size: 32 7260
PpuMI rG'GwC_Cy
Cuts at: 375 724 958 1780 1854 3258 3501 4065 375
Size: 349 234 822 74 1404 243 564 3602
Fragments arranged by size:
3602 1404 822 564 349 243 234 74
PspOMI G'GGCC_C
Cuts at: 603 636 1367 2481 2945 3794 603
Size: 33 731 1114 464 849 4101
Fragments arranged by size:
4101 1114 849 731 464 33
PstI C_TGCA'G
Cuts at: 815 842 2972 3014 3509 3818 4115 4339 5024
Size: 27 2130 42 495 309 297 224 685
Cuts at: 5024 815
Size: 3083
Fragments arranged by size:
3083 2130 685 495 309 297 224 42
27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7292
PvuII CAG'CTG
Cuts at: 178 201 1999 2850 3192 4717 5077 178
Size: 23 1798 851 342 1525 360 2393
Fragments arranged by size:
2393 1798 1525 851 360 342 23
RsrII CG'GwC_CG
Cuts at: 5487 5487
Size: 7292
SacI G_AGCT'C
Cuts at: 1541 2189 2234 3882 4078 4205 4306 1541
Size: 648 45 1648 196 127 101 4527
Fragments arranged by size:
4527 1648 648 196 127 101 45
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 7292
SalI G'TCGA_C
Cuts at: 4329 4329
Size: 7292
SanDI GG'GwC_CC
Cuts at: 724 724
Size: 7292
SapI GCTCTTCn'nnn_
Cuts at: 1508 5315 5525 1508
Size: 3807 210 3275
Fragments arranged by size:
3807 3275 210
SbfI CC_TGCA'GG
Cuts at: 842 4339 842
Size: 3497 3795
ScaI AGT'ACT
Cuts at: 6809 6809
Size: 7292
SfcI C'TryA_G
Cuts at: 33 811 838 1282 1883 2968 3010 3349 3505
Size: 778 27 444 601 1085 42 339 156
Cuts at: 3505 3814 4111 4335 5020 5988 6179 33
Size: 309 297 224 685 968 191 1146
Fragments arranged by size:
1146 1085 968 778 685 601 444 339
309 297 224 191 156 42 27
SfoI GGC'GCC
Cuts at: 3535 4724 4972 3535
Size: 1189 248 5855
Fragments arranged by size:
5855 1189 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7292
SmaI CCC'GGG
Cuts at: 2873 4314 2873
Size: 1441 5851
SmlI C'TyrA_G
Cuts at: 891 1410 3674 4447 5362 6076 6338 891
Size: 519 2264 773 915 714 262 1845
Fragments arranged by size:
2264 1845 915 773 714 519 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7292
SphI G_CATG'C
Cuts at: 4345 4845 4345
Size: 500 6792
SspI AAT'ATT
Cuts at: 2010 2010
Size: 7292
StuI AGG'CCT
Cuts at: 2530 3250 2530
Size: 720 6572
StyI C'CwwG_G
Cuts at: 599 676 818 977 1248 1457 1566 3056 3482
Size: 77 142 159 271 209 109 1490 426
Cuts at: 3482 4426 4780 599
Size: 944 354 3111
Fragments arranged by size:
3111 1490 944 426 354 271 209 159
142 109 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 684 1243 2254 2258 2311 3043 3115 3501
Size: 549 559 1011 4 53 732 72 386
Cuts at: 3501 4875 5008 5467 5676 135
Size: 1374 133 459 209 1751
Fragments arranged by size:
1751 1374 1011 732 559 549 459 386
209 133 72 53 4
TatI w'GTAC_w
Cuts at: 3630 6807 3630
Size: 3177 4115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1772 1804 3918 3950 6809 6841 1772
Size: 32 2114 32 2859 32 2223
Fragments arranged by size:
2859 2223 2114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1099 5089 1099
Size: 3990 3302
XbaI T'CTAG_A
Cuts at: 4323 4323
Size: 7292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1854 3480 4787 1854
Size: 1626 1307 4359
Fragments arranged by size:
4359 1626 1307
XmaI C'CCGG_G
Cuts at: 2871 4312 2871
Size: 1441 5851
XmnI GAAnn'nnTTC
Cuts at: 2887 2887
Size: 7292
ZraI GAC'GTC
Cuts at: 3722 3722
Size: 7292
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AloI AscI BaeI BfrBI BsiWI BsrGI BssHII
BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI