Restriction map for
pF1KE1032
(Circular) (Six-base) MAPSORT of: pF1KE1032.seq Check: 2785 from: 1 to: 7394
pF1KE1032 7394 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 735 4355 4432 735
Size: 3620 77 3697
Fragments arranged by size:
3697 3620 77
Acc65I G'GTAC_C
Cuts at: 4410 4410
Size: 7394
AclI AA'CG_TT
Cuts at: 7234 7234
Size: 7394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 2216 2465 2585 3203 4133 5219 5651 6004
Size: 1164 249 120 618 930 1086 432 353
Cuts at: 6004 6666 1052
Size: 662 1780
Fragments arranged by size:
1780 1164 1086 930 662 618 432 353
249 120
AflIII A'CryG_T
Cuts at: 1025 3291 4746 6546 6827 1025
Size: 2266 1455 1800 281 1592
Fragments arranged by size:
2266 1800 1592 1455 281
AleI CACnn'nnGTG
Cuts at: 3290 3290
Size: 7394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3054 3054
Size: 7394
AlwNI CAG_nnn'CTG
Cuts at: 2029 2221 2368 2962 3034 4699 6137 2029
Size: 192 147 594 72 1665 1438 3286
Fragments arranged by size:
3286 1665 1438 594 192 147 72
ApaLI G'TGCA_C
Cuts at: 6232 6232
Size: 7394
ApoI r'AATT_y
Cuts at: 596 798 913 997 1019 1350 1479 1764 1829
Size: 202 115 84 22 331 129 285 65
Cuts at: 1829 1973 2079 2784 3654 4278 4360 4398 6747
Size: 144 106 705 870 624 82 38 2349
Cuts at: 6747 7003 596
Size: 256 987
Fragments arranged by size:
2349 987 870 705 624 331 285 256
202 144 129 115 106 84 82 65
38 22
AseI AT'TA_AT
Cuts at: 20 1793 1853 2396 4111 20
Size: 1773 60 543 1715 3303
Fragments arranged by size:
3303 1773 1715 543 60
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7394
AvaI C'yCGr_G
Cuts at: 4414 4414
Size: 7394
AvrII C'CTAG_G
Cuts at: 1002 1002
Size: 7394
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 560 593 1385 1418 3770 3803 560
Size: 33 792 33 2352 33 4151
Fragments arranged by size:
4151 2352 792 33 33 33
BamHI G'GATC_C
Cuts at: 4419 4419
Size: 7394
BanI G'GyrC_C
Cuts at: 4410 4824 5072 5107 4410
Size: 414 248 35 6697
Fragments arranged by size:
6697 414 248 35
BanII G_rGCy'C
Cuts at: 441 4408 5438 441
Size: 3967 1030 2397
Fragments arranged by size:
3967 2397 1030
BbeI G_GCGC'C
Cuts at: 4828 5076 4828
Size: 248 7146
BbsI GAAGACnn'nnnn_
Cuts at: 1346 1571 1900 1988 2420 2573 4004 1346
Size: 225 329 88 432 153 1431 4736
Fragments arranged by size:
4736 1431 432 329 225 153 88
BciVI GTATCCnnnnn_n'
Cuts at: 3061 5292 6348 7394 3061
Size: 2231 1056 1046 3061
Fragments arranged by size:
3061 2231 1056 1046
BclI T'GATC_A
Cuts at: 1614 4914 1614
Size: 3300 4094
BfrBI ATG'CAT
Cuts at: 2308 2308
Size: 7394
BglII A'GATC_T
Cuts at: 3210 4909 6946 3210
Size: 1699 2037 3658
Fragments arranged by size:
3658 2037 1699
BlpI GC'TnA_GC
Cuts at: 4506 4506
Size: 7394
Bme1580I G_kGCm'C
Cuts at: 3911 5019 5112 6236 3911
Size: 1108 93 1124 5069
Fragments arranged by size:
5069 1124 1108 93
BmgBI CAC'GTC
Cuts at: 3292 3292
Size: 7394
BmrI ACTGGGnnnn_n'
Cuts at: 1169 2950 4322 4606 4687 5022 7166 1169
Size: 1781 1372 284 81 335 2144 1397
Fragments arranged by size:
2144 1781 1397 1372 335 284 81
BmtI G_CTAG'C
Cuts at: 898 898
Size: 7394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1616 4266 5761 1616
Size: 2650 1495 3249
Fragments arranged by size:
3249 2650 1495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 686 2159 2198 2900 4107 485
Size: 201 1473 39 702 1207 3772
Fragments arranged by size:
3772 1473 1207 702 201 39
Bpu10I CC'TnA_GC
Cuts at: 695 950 2060 3089 3924 695
Size: 255 1110 1029 835 4165
Fragments arranged by size:
4165 1110 1029 835 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4570 5449 6163 6461 4570
Size: 879 714 298 5503
Fragments arranged by size:
5503 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 7098 7098
Size: 7394
BsaAI yAC'GTr
Cuts at: 4747 4747
Size: 7394
BsaBI GATnn'nnATC
Cuts at: 6 4908 6945 6
Size: 4902 2037 455
Fragments arranged by size:
4902 2037 455
BsaHI Gr'CG_yC
Cuts at: 2170 4825 5073 5775 7271 2170
Size: 2655 248 702 1496 2293
Fragments arranged by size:
2655 2293 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4583 5104 5782 6193 6340 6614 4583
Size: 521 678 411 147 274 5363
Fragments arranged by size:
5363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 540 570 3303 3333 540
Size: 30 2733 30 4601
Fragments arranged by size:
4601 2733 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 545 2987 2990 3464 4148 545
Size: 2442 3 474 684 3791
Fragments arranged by size:
3791 2442 684 474 3
BseYI C'CCAG_C
Cuts at: 342 1074 4819 5577 6242 6650 342
Size: 732 3745 758 665 408 1086
Fragments arranged by size:
3745 1086 758 732 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 2774 530
Size: 2244 5150
BsiEI CG_ry'CG
Cuts at: 104 4936 4982 6212 104
Size: 4832 46 1230 1286
Fragments arranged by size:
4832 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 696 4204 4408 5186 5376 6236 696
Size: 3508 204 778 190 860 1854
Fragments arranged by size:
3508 1854 860 778 204 190
BsmI GAATG_Cn'
Cuts at: 3384 3384
Size: 7394
BsmBI CGTCTCn'nnnn_
Cuts at: 2325 2325
Size: 7394
Bsp1286I G_dGCh'C
Cuts at: 441 696 3911 4204 4408 5019 5112 5186 5376
Size: 255 3215 293 204 611 93 74 190
Cuts at: 5376 5438 6236 441
Size: 62 798 1599
Fragments arranged by size:
3215 1599 798 611 293 255 204 190
93 74 62
BspHI T'CATG_A
Cuts at: 5826 5826
Size: 7394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 184 2417 2997 4444 4658 4960 5341 184
Size: 2233 580 1447 214 302 381 2237
Fragments arranged by size:
2237 2233 1447 580 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5686 5740 7224 7389 50
Size: 5636 54 1484 165 55
Fragments arranged by size:
5636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2603 4372 5306 2603
Size: 1769 934 4691
Fragments arranged by size:
4691 1769 934
BsrFI r'CCGG_y
Cuts at: 3515 5392 5573 3515
Size: 1877 181 5336
Fragments arranged by size:
5336 1877 181
BsrGI T'GTAC_A
Cuts at: 229 1267 229
Size: 1038 6356
BssSI C'ACGA_G
Cuts at: 854 5665 6373 854
Size: 4811 708 1875
Fragments arranged by size:
4811 1875 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4771 4771
Size: 7394
BstBI TT'CG_AA
Cuts at: 5755 5755
Size: 7394
BstYI r'GATC_y
Cuts at: 1 603 807 2893 3210 3792 4419 4886 4909
Size: 602 204 2086 317 582 627 467 23
Cuts at: 4909 5244 5490 5796 5808 5894 6946 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
2086 1052 627 602 582 467 449 335
317 306 246 204 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3641 5344 5525 5688 3641
Size: 1703 181 163 5347
Fragments arranged by size:
5347 1703 181 163
BtsI GCAGTG_nn'
Cuts at: 3715 4637 3715
Size: 922 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3195 3230 3195
Size: 35 7359
DraI TTT'AAA
Cuts at: 209 296 677 1392 1952 4393 209
Size: 87 381 715 560 2441 3210
Fragments arranged by size:
3210 2441 715 560 381 87
DraIII CAC_nnn'GTG
Cuts at: 1507 2632 1507
Size: 1125 6269
DrdI GACnn_nn'nnGTC
Cuts at: 5100 6444 5100
Size: 1344 6050
EaeI y'GGCC_r
Cuts at: 591 4979 5153 5544 5571 6916 591
Size: 4388 174 391 27 1345 1069
Fragments arranged by size:
4388 1345 1069 391 174 27
EagI C'GGCC_G
Cuts at: 4979 4979
Size: 7394
EarI CTCTTCn'nnn_
Cuts at: 887 1687 2105 2213 3248 5417 5627 887
Size: 800 418 108 1035 2169 210 2654
Fragments arranged by size:
2654 2169 1035 800 418 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3807 5602 6344 6490 7204 3807
Size: 1795 742 146 714 3997
Fragments arranged by size:
3997 1795 742 714 146
EcoICRI GAG'CTC
Cuts at: 4406 4406
Size: 7394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 686 1052 2159 2198 2216 2465 2585 2900
Size: 201 366 1107 39 18 249 120 315
Cuts at: 2900 3203 4107 4133 5219 5651 6004 6666 485
Size: 303 904 26 1086 432 353 662 1213
Fragments arranged by size:
1213 1107 1086 904 662 432 366 353
315 303 249 201 120 39 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 563 2177 3113 3923 3981 563
Size: 1614 936 810 58 3976
Fragments arranged by size:
3976 1614 936 810 58
EcoO109I rG'GnC_Cy
Cuts at: 3972 4533 3972
Size: 561 6833
EcoRI G'AATT_C
Cuts at: 997 1479 2079 4398 997
Size: 482 600 2319 3993
Fragments arranged by size:
3993 2319 600 482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 785 2699 5413 785
Size: 1914 2714 2766
Fragments arranged by size:
2766 2714 1914
FspI TGC'GCA
Cuts at: 5175 5175
Size: 7394
HaeII r_GCGC'y
Cuts at: 4828 5076 6306 7077 4828
Size: 248 1230 771 5145
Fragments arranged by size:
5145 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1231 1263 1978 2010 2011 2043 2080 2112 2515
Size: 32 715 32 1 32 37 32 403
Cuts at: 2515 2547 3281 3313 5236 5266 5268 5298 1231
Size: 32 734 32 1923 30 2 30 3327
Fragments arranged by size:
3327 1923 734 715 403 37 32 32
32 32 32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 633 1156 2862 4433 633
Size: 523 1706 1571 3594
Fragments arranged by size:
3594 1706 1571 523
HindIII A'AGCT_T
Cuts at: 481 671 4321 481
Size: 190 3650 3554
Fragments arranged by size:
3650 3554 190
KasI G'GCGC_C
Cuts at: 4824 5072 4824
Size: 248 7146
KpnI G_GTAC'C
Cuts at: 4414 4414
Size: 7394
MfeI C'AATT_G
Cuts at: 989 989
Size: 7394
MluI A'CGCG_T
Cuts at: 6827 6827
Size: 7394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2755 2949 3141 6153 6337 2755
Size: 194 192 3012 184 3812
Fragments arranged by size:
3812 3012 194 192 184
MscI TGG'CCA
Cuts at: 593 5155 593
Size: 4562 2832
MslI CAynn'nnrTG
Cuts at: 360 1952 3036 3290 3841 4111 4382 5383 5510
Size: 1592 1084 254 551 270 271 1001 127
Cuts at: 5510 360
Size: 2244
Fragments arranged by size:
2244 1592 1084 1001 551 271 270 254
127
MspA1I CmG'CkG
Cuts at: 1399 3271 3784 4505 4819 5179 5963 6208 1399
Size: 1872 513 721 314 360 784 245 2585
Fragments arranged by size:
2585 1872 784 721 513 360 314 245
NaeI GCC'GGC
Cuts at: 3517 5575 3517
Size: 2058 5336
NarI GG'CG_CC
Cuts at: 4825 5073 4825
Size: 248 7146
NdeI CA'TA_TG
Cuts at: 3017 3017
Size: 7394
NgoMIV G'CCGG_C
Cuts at: 3515 5573 3515
Size: 2058 5336
NheI G'CTAG_C
Cuts at: 894 894
Size: 7394
NsiI A_TGCA'T
Cuts at: 2310 2310
Size: 7394
NspI r_CATG'y
Cuts at: 405 715 1029 4447 4947 6550 405
Size: 310 314 3418 500 1603 1249
Fragments arranged by size:
3418 1603 1249 500 314 310
PciI A'CATG_T
Cuts at: 1025 6546 1025
Size: 5521 1873
PflMI CCAn_nnn'nTGG
Cuts at: 2940 3354 2940
Size: 414 6980
PmeI GTTT'AAAC
Cuts at: 4393 4393
Size: 7394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5840 5872 5840
Size: 32 7362
PstI C_TGCA'G
Cuts at: 2577 4057 4441 5126 2577
Size: 1480 384 685 4845
Fragments arranged by size:
4845 1480 685 384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7394
PvuII CAG'CTG
Cuts at: 1399 3271 3784 4819 5179 1399
Size: 1872 513 1035 360 3614
Fragments arranged by size:
3614 1872 1035 513 360
RsrII CG'GwC_CG
Cuts at: 5589 5589
Size: 7394
SacI G_AGCT'C
Cuts at: 4408 4408
Size: 7394
SalI G'TCGA_C
Cuts at: 4431 4431
Size: 7394
SapI GCTCTTCn'nnn_
Cuts at: 5417 5627 5417
Size: 210 7184
SbfI CC_TGCA'GG
Cuts at: 4441 4441
Size: 7394
ScaI AGT'ACT
Cuts at: 6911 6911
Size: 7394
SfcI C'TryA_G
Cuts at: 33 1553 2189 2492 2573 3707 3956 4053 4437
Size: 1520 636 303 81 1134 249 97 384
Cuts at: 4437 5122 6090 6281 33
Size: 685 968 191 1146
Fragments arranged by size:
1520 1146 1134 968 685 636 384 303
249 191 97 81
SfoI GGC'GCC
Cuts at: 4826 5074 4826
Size: 248 7146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7394
SmaI CCC'GGG
Cuts at: 4416 4416
Size: 7394
SmlI C'TyrA_G
Cuts at: 4549 5464 6178 6440 4549
Size: 915 714 262 5503
Fragments arranged by size:
5503 915 714 262
SpeI A'CTAG_T
Cuts at: 67 922 2463 67
Size: 855 1541 4998
Fragments arranged by size:
4998 1541 855
SphI G_CATG'C
Cuts at: 405 4447 4947 405
Size: 4042 500 2852
Fragments arranged by size:
4042 2852 500
SspI AAT'ATT
Cuts at: 936 936
Size: 7394
StuI AGG'CCT
Cuts at: 1387 1387
Size: 7394
StyI C'CwwG_G
Cuts at: 415 1002 2639 2836 2934 4528 4882 415
Size: 587 1637 197 98 1594 354 2927
Fragments arranged by size:
2927 1637 1594 587 354 197 98
SwaI ATTT'AAAT
Cuts at: 1952 1952
Size: 7394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3423 4977 5110 5569 5778 3423
Size: 1554 133 459 209 5039
Fragments arranged by size:
5039 1554 459 209 133
TatI w'GTAC_w
Cuts at: 229 1267 2928 3294 3765 4006 6909 229
Size: 1038 1661 366 471 241 2903 714
Fragments arranged by size:
2903 1661 1038 714 471 366 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2239 2271 3303 3335 6911 6943 2239
Size: 32 1032 32 3576 32 2690
Fragments arranged by size:
3576 2690 1032 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5191 5191
Size: 7394
XbaI T'CTAG_A
Cuts at: 4425 4425
Size: 7394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1158 4889 1158
Size: 3731 3663
XmaI C'CCGG_G
Cuts at: 4414 4414
Size: 7394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NsiI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI BbvCI BcgI BglI BsiWI BspEI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI EcoRV FseI FspAI
HpaI NcoI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI XhoI XmnI ZraI