Restriction map for
pF1KE1035
(Circular) (Six-base) MAPSORT of: pF1KE1035.seq Check: 9908 from: 1 to: 4397
pF1KE1035 4397 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 435 435
Size: 4397
AccI GT'mk_AC
Cuts at: 290 1435 290
Size: 1145 3252
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 934 2222 2654 3401 3669 610
Size: 324 1288 432 747 268 1338
Fragments arranged by size:
1338 1288 747 432 324 268
AfeI AGC'GCT
Cuts at: 511 511
Size: 4397
AflIII A'CryG_T
Cuts at: 1749 2853 3830 1749
Size: 1104 977 2316
Fragments arranged by size:
2316 1104 977
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AleI CACnn'nnGTG
Cuts at: 1276 1276
Size: 4397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 850 850
Size: 4397
AlwNI CAG_nnn'CTG
Cuts at: 172 439 1702 3269 172
Size: 267 1263 1567 1300
Fragments arranged by size:
1567 1300 1263 267
ApaLI G'TGCA_C
Cuts at: 3167 3167
Size: 4397
ApoI r'AATT_y
Cuts at: 746 1124 1401 3750 4006 746
Size: 378 277 2349 256 1137
Fragments arranged by size:
2349 1137 378 277 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 473 1417 473
Size: 944 3453
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 1252 1413 1827 2075 2110 1252
Size: 161 414 248 35 3539
Fragments arranged by size:
3539 414 248 161 35
BanII G_rGCy'C
Cuts at: 137 220 1411 2441 137
Size: 83 1191 1030 2093
Fragments arranged by size:
2093 1191 1030 83
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BbvCI CC'TCA_GC
Cuts at: 188 188
Size: 4397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1146 1180 1146
Size: 34 4363
BciVI GTATCCnnnnn_n'
Cuts at: 643 2295 3056 4397 643
Size: 1652 761 1341 643
Fragments arranged by size:
1652 1341 761 643
BclI T'GATC_A
Cuts at: 1917 1917
Size: 4397
BglI GCCn_nnn'nGGC
Cuts at: 1250 1250
Size: 4397
BglII A'GATC_T
Cuts at: 851 1912 3949 851
Size: 1061 2037 1299
Fragments arranged by size:
2037 1299 1061
BlpI GC'TnA_GC
Cuts at: 1509 1509
Size: 4397
Bme1580I G_kGCm'C
Cuts at: 2022 2115 3171 2022
Size: 93 1056 3248
Fragments arranged by size:
3248 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 449 1609 1690 2025 4169 449
Size: 1160 81 335 2144 677
Fragments arranged by size:
2144 1160 677 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 531 1106 2764 531
Size: 575 1658 2164
Fragments arranged by size:
2164 1658 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 608 448
Size: 160 4237
Bpu10I CC'TnA_GC
Cuts at: 188 188
Size: 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 819 982 1573 2452 2944 3242 247
Size: 572 163 591 879 492 298 1402
Fragments arranged by size:
1402 879 591 572 492 298 163
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 906 1750 906
Size: 844 3553
BsaBI GATnn'nnATC
Cuts at: 6 1363 1911 3948 6
Size: 1357 548 2037 455
Fragments arranged by size:
2037 1357 548 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 932 1586 2107 2785 3059 3206 3617 932
Size: 654 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 654 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1354 1384 1354
Size: 30 4367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1093 1359 1093
Size: 266 4131
BseYI C'CCAG_C
Cuts at: 172 1019 1044 1822 2580 3157 3653 172
Size: 847 25 778 758 577 496 916
Fragments arranged by size:
916 847 778 758 577 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1336 1018
Size: 318 4079
BsiEI CG_ry'CG
Cuts at: 104 1939 1985 3193 104
Size: 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 1411 2189 2379 3171 137
Size: 1274 778 190 792 1363
Fragments arranged by size:
1363 1274 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 862 862
Size: 4397
Bsp1286I G_dGCh'C
Cuts at: 137 220 1411 2022 2115 2189 2379 2441 3171
Size: 83 1191 611 93 74 190 62 730
Cuts at: 3171 137
Size: 1363
Fragments arranged by size:
1363 1191 730 611 190 93 83 74
62
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 779 3573 779
Size: 2794 1603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 435 765 1229 1447 1661 1963 2344 435
Size: 330 464 218 214 302 381 2488
Fragments arranged by size:
2488 464 381 330 302 218 214
BsrBI CCG'CTC
Cuts at: 50 1170 2689 2743 4227 4392 50
Size: 1120 1519 54 1484 165 55
Fragments arranged by size:
1519 1484 1120 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 1049 1178 2309 1007
Size: 42 129 1131 3095
Fragments arranged by size:
3095 1131 129 42
BsrFI r'CCGG_y
Cuts at: 1244 2395 2576 2785 1244
Size: 1151 181 209 2856
Fragments arranged by size:
2856 1151 209 181
BssSI C'ACGA_G
Cuts at: 766 2668 3026 766
Size: 1902 358 2137
Fragments arranged by size:
2137 1902 358
BstAPI GCAn_nnn'nTGC
Cuts at: 891 1774 891
Size: 883 3514
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstXI CCAn_nnnn'nTGG
Cuts at: 241 1263 241
Size: 1022 3375
BstYI r'GATC_y
Cuts at: 1 809 840 851 1301 1422 1889 1912 2247
Size: 808 31 11 450 121 467 23 335
Cuts at: 2247 2493 3505 3591 3603 3949 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 808 467 450 449 346 335 246
121 86 31 23 12 11
Bsu36I CC'TnA_GG
Cuts at: 338 1202 338
Size: 864 3533
BtgI C'CryG_G
Cuts at: 107 1062 107
Size: 955 3442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2347 2528 2691 2347
Size: 181 163 4053
Fragments arranged by size:
4053 181 163
BtsI GCAGTG_nn'
Cuts at: 404 415 1640 404
Size: 11 1225 3161
Fragments arranged by size:
3161 1225 11
DraI TTT'AAA
Cuts at: 1107 1396 1107
Size: 289 4108
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 410 1149 1232 1982 2156 2547 2574 3919 410
Size: 739 83 750 174 391 27 1345 888
Fragments arranged by size:
1345 888 750 739 391 174 83 27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 575 2420 2630 575
Size: 1845 210 2342
Fragments arranged by size:
2342 1845 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2605 2915 3061 4207 2605
Size: 310 146 1146 2795
Fragments arranged by size:
2795 1146 310 146
EcoICRI GAG'CTC
Cuts at: 135 1409 135
Size: 1274 3123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 608 610 934 2222 2654 3401 3669 448
Size: 160 2 324 1288 432 747 268 1176
Fragments arranged by size:
1288 1176 747 432 324 268 160 2
EcoNI CCTnn'n_nnAGG
Cuts at: 367 367
Size: 4397
EcoO109I rG'GnC_Cy
Cuts at: 192 1536 192
Size: 1344 3053
EcoRI G'AATT_C
Cuts at: 1124 1401 1124
Size: 277 4120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4397
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 513 1831 2079 3101 4080 513
Size: 1318 248 1022 979 830
Fragments arranged by size:
1318 1022 979 830 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 1354 1386 2239 2269 2271 2301 295
Size: 32 1027 32 853 30 2 30 2391
Fragments arranged by size:
2391 1027 853 32 32 30 30 2
HincII GTy'rAC
Cuts at: 291 878 1436 291
Size: 587 558 3252
Fragments arranged by size:
3252 587 558
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3252 3068
Size: 184 4213
MscI TGG'CCA
Cuts at: 412 1234 2158 412
Size: 822 924 2651
Fragments arranged by size:
2651 924 822
MslI CAynn'nnrTG
Cuts at: 1276 2386 2513 1276
Size: 1110 127 3160
Fragments arranged by size:
3160 1110 127
MspA1I CmG'CkG
Cuts at: 172 426 1002 1508 1822 2182 3195 3440 172
Size: 254 576 506 314 360 1013 245 1129
Fragments arranged by size:
1129 1013 576 506 360 314 254 245
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 4397
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 107
Size: 4397
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 4397
NspI r_CATG'y
Cuts at: 528 729 1200 1450 1950 2857 528
Size: 201 471 250 500 907 2068
Fragments arranged by size:
2068 907 500 471 250 201
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PflMI CCAn_nnn'nTGG
Cuts at: 439 439
Size: 4397
PfoI T'CCnGG_A
Cuts at: 740 740
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PshAI GACnn'nnGTC
Cuts at: 728 728
Size: 4397
PstI C_TGCA'G
Cuts at: 1444 2129 1444
Size: 685 3712
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 172 426 1822 2182 172
Size: 254 1396 360 2387
Fragments arranged by size:
2387 1396 360 254
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 137 1411 137
Size: 1274 3123
SalI G'TCGA_C
Cuts at: 289 1434 289
Size: 1145 3252
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 901 3914 901
Size: 3013 1384
SexAI A'CCwGG_T
Cuts at: 971 971
Size: 4397
SfcI C'TryA_G
Cuts at: 33 1440 2125 3118 3309 33
Size: 1407 685 993 191 1121
Fragments arranged by size:
1407 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 1419 1419
Size: 4397
SmlI C'TyrA_G
Cuts at: 262 798 961 1552 2467 2959 3221 262
Size: 536 163 591 915 492 262 1438
Fragments arranged by size:
1438 915 591 536 492 262 163
SpeI A'CTAG_T
Cuts at: 67 1026 67
Size: 959 3438
SphI G_CATG'C
Cuts at: 1450 1950 1450
Size: 500 3897
SspI AAT'ATT
Cuts at: 1338 1338
Size: 4397
StyI C'CwwG_G
Cuts at: 107 1013 1256 1531 1885 107
Size: 906 243 275 354 2619
Fragments arranged by size:
2619 906 354 275 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1980 2113 2572 2781 1980
Size: 133 459 209 3596
Fragments arranged by size:
3596 459 209 133
TatI w'GTAC_w
Cuts at: 899 3912 899
Size: 3013 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 3914 3946 349
Size: 32 3533 32 800
Fragments arranged by size:
3533 800 32 32
Tth111I GACn'n_nGTC
Cuts at: 2194 2194
Size: 4397
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 351 381 1892 351
Size: 30 1511 2856
Fragments arranged by size:
2856 1511 30
XmaI C'CCGG_G
Cuts at: 1417 1417
Size: 4397
XmnI GAAnn'nnTTC
Cuts at: 749 749
Size: 4397
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI