Restriction map for
pF1KE1039
(Circular) (Six-base) MAPSORT of: pF1KE1039.seq Check: 6958 from: 1 to: 4598
pF1KE1039 4598 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2855 3602 3870 2423
Size: 432 747 268 3151
Fragments arranged by size:
3151 747 432 268
AflIII A'CryG_T
Cuts at: 622 956 1950 3054 4031 622
Size: 334 994 1104 977 1189
Fragments arranged by size:
1189 1104 994 977 334
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 1000 1164 1903 3470 1000
Size: 164 739 1567 2128
Fragments arranged by size:
2128 1567 739 164
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 185 262 363 574 946 1153 1602 3951 4207
Size: 77 101 211 372 207 449 2349 256
Cuts at: 4207 185
Size: 576
Fragments arranged by size:
2349 576 449 372 256 211 207 101
77
AseI AT'TA_AT
Cuts at: 20 755 20
Size: 735 3863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 1618 1618
Size: 4598
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 938 1614 2028 2276 2311 938
Size: 676 414 248 35 3225
Fragments arranged by size:
3225 676 414 248 35
BanII G_rGCy'C
Cuts at: 601 1612 2642 601
Size: 1011 1030 2557
Fragments arranged by size:
2557 1030 1011
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BbsI GAAGACnn'nnnn_
Cuts at: 163 1039 163
Size: 876 3722
BciVI GTATCCnnnnn_n'
Cuts at: 1180 2496 3257 4598 1180
Size: 1316 761 1341 1180
Fragments arranged by size:
1341 1316 1180 761
BclI T'GATC_A
Cuts at: 518 2118 518
Size: 1600 2998
BfrBI ATG'CAT
Cuts at: 1271 1271
Size: 4598
BglII A'GATC_T
Cuts at: 1452 2113 4150 1452
Size: 661 2037 1900
Fragments arranged by size:
2037 1900 661
BlpI GC'TnA_GC
Cuts at: 662 1710 662
Size: 1048 3550
Bme1580I G_kGCm'C
Cuts at: 2223 2316 3372 2223
Size: 93 1056 3449
Fragments arranged by size:
3449 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1810 1891 2226 4370 1810
Size: 81 335 2144 2038
Fragments arranged by size:
2144 2038 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 696 2965 696
Size: 2269 2329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 629 456
Size: 173 4425
Bpu10I CC'TnA_GC
Cuts at: 671 671
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2653 3145 3443 1774
Size: 879 492 298 2929
Fragments arranged by size:
2929 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 2112 4149 6
Size: 2106 2037 455
Fragments arranged by size:
2106 2037 455
BsaHI Gr'CG_yC
Cuts at: 2029 2277 2979 4475 2029
Size: 248 702 1496 2152
Fragments arranged by size:
2152 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3260 3407 3818 1787
Size: 521 678 274 147 411 2567
Fragments arranged by size:
2567 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2023 2781 3358 3854 2023
Size: 758 577 496 2767
Fragments arranged by size:
2767 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3394 104
Size: 2036 46 1208 1308
Fragments arranged by size:
2036 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1612 2390 2580 3372 1612
Size: 778 190 792 2838
Fragments arranged by size:
2838 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 601 1612 2223 2316 2390 2580 2642 3372 601
Size: 1011 611 93 74 190 62 730 1827
Fragments arranged by size:
1827 1011 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3818 3818
Size: 4598
BspHI T'CATG_A
Cuts at: 152 3774 152
Size: 3622 976
BspMI ACCTGCnnnn'nnnn_
Cuts at: 488 1648 1862 2164 2545 488
Size: 1160 214 302 381 2541
Fragments arranged by size:
2541 1160 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 218 2510 206
Size: 12 2292 2294
Fragments arranged by size:
2294 2292 12
BsrFI r'CCGG_y
Cuts at: 2596 2777 2986 2596
Size: 181 209 4208
Fragments arranged by size:
4208 209 181
BsrGI T'GTAC_A
Cuts at: 951 959 951
Size: 8 4590
BssSI C'ACGA_G
Cuts at: 2869 3227 2869
Size: 358 4240
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstYI r'GATC_y
Cuts at: 1 318 648 1452 1623 2090 2113 2448 2694
Size: 317 330 804 171 467 23 335 246
Cuts at: 2694 3706 3792 3804 4150 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 804 467 449 346 335 330 317
246 171 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 989 2548 2729 2892 989
Size: 1559 181 163 2695
Fragments arranged by size:
2695 1559 181 163
BtsI GCAGTG_nn'
Cuts at: 1841 1841
Size: 4598
DraI TTT'AAA
Cuts at: 485 875 1597 485
Size: 390 722 3486
Fragments arranged by size:
3486 722 390
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 119 2183 2357 2748 2775 4120 119
Size: 2064 174 391 27 1345 597
Fragments arranged by size:
2064 1345 597 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 435 629 782 1355 1460 2621 2831 435
Size: 194 153 573 105 1161 210 2202
Fragments arranged by size:
2202 1161 573 210 194 153 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2806 3116 3262 4408 2806
Size: 310 146 1146 2996
Fragments arranged by size:
2996 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1610 1610
Size: 4598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 629 2423 2855 3602 3870 456
Size: 173 1794 432 747 268 1184
Fragments arranged by size:
1794 1184 747 432 268 173
EcoO109I rG'GnC_Cy
Cuts at: 1737 1737
Size: 4598
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1414 2617 1414
Size: 1203 3395
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3302 4281 2032
Size: 248 1022 979 2349
Fragments arranged by size:
2349 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 207 239 2440 2470 2472 2502 207
Size: 32 2201 30 2 30 2303
Fragments arranged by size:
2303 2201 32 30 30 2
HincII GTy'rAC
Cuts at: 735 1637 735
Size: 902 3696
HindIII A'AGCT_T
Cuts at: 357 357
Size: 4598
HpaI GTT'AAC
Cuts at: 735 735
Size: 4598
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 313 3269 3453 313
Size: 2956 184 1458
Fragments arranged by size:
2956 1458 184
MscI TGG'CCA
Cuts at: 2359 2359
Size: 4598
MslI CAynn'nnrTG
Cuts at: 211 1148 2587 2714 211
Size: 937 1439 127 2095
Fragments arranged by size:
2095 1439 937 127
MspA1I CmG'CkG
Cuts at: 773 834 997 1549 1709 2023 2383 3396 3641
Size: 61 163 552 160 314 360 1013 245
Cuts at: 3641 773
Size: 1730
Fragments arranged by size:
1730 1013 552 360 314 245 163 160
61
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NcoI C'CATG_G
Cuts at: 107 107
Size: 4598
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NsiI A_TGCA'T
Cuts at: 1273 1273
Size: 4598
NspI r_CATG'y
Cuts at: 960 1651 2151 3058 960
Size: 691 500 907 2500
Fragments arranged by size:
2500 907 691 500
PciI A'CATG_T
Cuts at: 956 3054 956
Size: 2098 2500
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PstI C_TGCA'G
Cuts at: 389 1645 2330 389
Size: 1256 685 2657
Fragments arranged by size:
2657 1256 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 773 997 2023 2383 773
Size: 224 1026 360 2988
Fragments arranged by size:
2988 1026 360 224
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 1612 1612
Size: 4598
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 2621 2831 2621
Size: 210 4388
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 385 464 629 1247 1641 2326 3319 3510
Size: 352 79 165 618 394 685 993 191
Cuts at: 3510 33
Size: 1121
Fragments arranged by size:
1121 993 685 618 394 352 191 165
79
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 1620 1620
Size: 4598
SmlI C'TyrA_G
Cuts at: 1753 2668 3160 3422 1753
Size: 915 492 262 2929
Fragments arranged by size:
2929 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 1651 2151 1651
Size: 500 4098
StyI C'CwwG_G
Cuts at: 107 1732 2086 107
Size: 1625 354 2619
Fragments arranged by size:
2619 1625 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2181 2314 2773 2982 2181
Size: 133 459 209 3797
Fragments arranged by size:
3797 459 209 133
TatI w'GTAC_w
Cuts at: 951 959 4113 951
Size: 8 3154 1436
Fragments arranged by size:
3154 1436 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4566
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2093 114
Size: 1979 2619
XmaI C'CCGG_G
Cuts at: 1618 1618
Size: 4598
XmnI GAAnn'nnTTC
Cuts at: 1007 1007
Size: 4598
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI