Restriction map for
pF1KE1041
(Circular) (Six-base) MAPSORT of: pF1KE1041.seq Check: 5703 from: 1 to: 4163
pF1KE1041 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 1157 4003 1157
Size: 2846 1317
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 199 410 560 922 1055 1988 2420 3167
Size: 18 211 150 362 133 933 432 747
Cuts at: 3167 3435 181
Size: 268 909
Fragments arranged by size:
933 909 747 432 362 268 211 150
133 18
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AhdI GACnn_n'nnGTC
Cuts at: 858 858
Size: 4163
AleI CACnn'nnGTG
Cuts at: 923 923
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 463 1468 3035 463
Size: 1005 1567 1591
Fragments arranged by size:
1591 1567 1005
ApaI G_GGCC'C
Cuts at: 421 421
Size: 4163
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 868 1167 3516 3772 868
Size: 299 2349 256 1259
Fragments arranged by size:
2349 1259 299 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 421 1177 2207 421
Size: 756 1030 2377
Fragments arranged by size:
2377 1030 756
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 970 970
Size: 4163
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 187 421 1788 1881 2937 187
Size: 234 1367 93 1056 1413
Fragments arranged by size:
1413 1367 1056 234 93
BmgBI CAC'GTC
Cuts at: 709 709
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1168 2530 1168
Size: 1362 2801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1040 1079 566
Size: 474 39 3650
Fragments arranged by size:
3650 474 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 1339 2218 2710 3008 603
Size: 736 879 492 298 1758
Fragments arranged by size:
1758 879 736 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 836 1516 836
Size: 680 3483
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 713 1092 1588 2346 2923 3419 713
Size: 379 496 758 577 496 1457
Fragments arranged by size:
1457 758 577 496 496 379
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
BsmI GAATG_Cn'
Cuts at: 616 616
Size: 4163
Bsp1286I G_dGCh'C
Cuts at: 187 421 1177 1788 1881 1955 2145 2207 2937
Size: 234 756 611 93 74 190 62 730
Cuts at: 2937 187
Size: 1413
Fragments arranged by size:
1413 756 730 611 234 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 1213 1427 1729 2110 297
Size: 916 214 302 381 2350
Fragments arranged by size:
2350 916 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 293 708 903 2075 293
Size: 415 195 1172 2381
Fragments arranged by size:
2381 1172 415 195
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BsrGI T'GTAC_A
Cuts at: 657 657
Size: 4163
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 512 1540 512
Size: 1028 3135
BstBI TT'CG_AA
Cuts at: 693 2524 693
Size: 1831 2332
BstEII G'GTnAC_C
Cuts at: 1147 1147
Size: 4163
BstYI r'GATC_y
Cuts at: 1 1188 1655 1678 2013 2259 3271 3357 3369
Size: 1187 467 23 335 246 1012 86 12
Cuts at: 3369 3715 1
Size: 346 449
Fragments arranged by size:
1187 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 690 725 690
Size: 35 4128
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 139 1748 1922 2313 2340 3685 139
Size: 1609 174 391 27 1345 617
Fragments arranged by size:
1609 1345 617 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 1019 2186 2396 1019
Size: 1167 210 2786
Fragments arranged by size:
2786 1167 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 199 410 560 566 922 1040 1055 1079
Size: 18 211 150 6 356 118 15 24
Cuts at: 1079 1988 2420 3167 3435 181
Size: 909 432 747 268 909
Fragments arranged by size:
909 909 747 432 356 268 211 150
118 24 18 15 6
EcoO109I rG'GnC_Cy
Cuts at: 417 1302 417
Size: 885 3278
EcoRI G'AATT_C
Cuts at: 868 1167 868
Size: 299 3864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 764 796 844 876 2005 2035 2037 2067 764
Size: 32 48 32 1129 30 2 30 2860
Fragments arranged by size:
2860 1129 48 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 750 2834 3018 750
Size: 2084 184 1895
Fragments arranged by size:
2084 1895 184
MscI TGG'CCA
Cuts at: 141 1924 141
Size: 1783 2380
MslI CAynn'nnrTG
Cuts at: 923 2152 2279 923
Size: 1229 127 2807
Fragments arranged by size:
2807 1229 127
MspA1I CmG'CkG
Cuts at: 923 1274 1588 1948 2961 3206 923
Size: 351 314 360 1013 245 1880
Fragments arranged by size:
1880 1013 360 351 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 463 463
Size: 4163
PfoI T'CCnGG_A
Cuts at: 462 462
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PsiI TTA'TAA
Cuts at: 563 563
Size: 4163
PspOMI G'GGCC_C
Cuts at: 417 417
Size: 4163
PstI C_TGCA'G
Cuts at: 341 1210 1895 341
Size: 869 685 2609
Fragments arranged by size:
2609 869 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 923 1588 1948 923
Size: 665 360 3138
Fragments arranged by size:
3138 665 360
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 1019 2186 2396 1019
Size: 1167 210 2786
Fragments arranged by size:
2786 1167 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SexAI A'CCwGG_T
Cuts at: 591 591
Size: 4163
SfcI C'TryA_G
Cuts at: 33 337 1206 1891 2884 3075 33
Size: 304 869 685 993 191 1121
Fragments arranged by size:
1121 993 869 685 304 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 618 1318 2233 2725 2987 618
Size: 700 915 492 262 1794
Fragments arranged by size:
1794 915 700 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
SspI AAT'ATT
Cuts at: 115 115
Size: 4163
StyI C'CwwG_G
Cuts at: 107 1297 1651 107
Size: 1190 354 2619
Fragments arranged by size:
2619 1190 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 302 657 3678 302
Size: 355 3021 787
Fragments arranged by size:
3021 787 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1658 1658
Size: 4163
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI