Restriction map for
pF1KE1042
(Circular) (Six-base) MAPSORT of: pF1KE1042.seq Check: 72 from: 1 to: 4616
pF1KE1042 4616 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1632 1632
Size: 4616
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1431 2441 2873 3620 3888 1431
Size: 1010 432 747 268 2159
Fragments arranged by size:
2159 1010 747 432 268
AflIII A'CryG_T
Cuts at: 1968 3072 4049 1968
Size: 1104 977 2535
Fragments arranged by size:
2535 1104 977
AgeI A'CCGG_T
Cuts at: 260 3004 260
Size: 2744 1872
AhdI GACnn_n'nnGTC
Cuts at: 512 512
Size: 4616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 1527 633
Size: 894 3722
AlwNI CAG_nnn'CTG
Cuts at: 825 1077 1921 3488 825
Size: 252 844 1567 1953
Fragments arranged by size:
1953 1567 844 252
ApaI G_GGCC'C
Cuts at: 1357 1357
Size: 4616
ApaLI G'TGCA_C
Cuts at: 3386 3386
Size: 4616
ApoI r'AATT_y
Cuts at: 1551 1620 3969 4225 1551
Size: 69 2349 256 1942
Fragments arranged by size:
2349 1942 256 69
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 701 778 1636 701
Size: 77 858 3681
Fragments arranged by size:
3681 858 77
BamHI G'GATC_C
Cuts at: 1641 1641
Size: 4616
BanI G'GyrC_C
Cuts at: 609 1306 1632 2046 2294 2329 609
Size: 697 326 414 248 35 2896
Fragments arranged by size:
2896 697 414 326 248 35
BanII G_rGCy'C
Cuts at: 856 911 1357 1610 1630 2660 856
Size: 55 446 253 20 1030 2812
Fragments arranged by size:
2812 1030 446 253 55 20
BbeI G_GCGC'C
Cuts at: 2050 2298 2050
Size: 248 4368
BbsI GAAGACnn'nnnn_
Cuts at: 946 1158 946
Size: 212 4404
BbvCI CC'TCA_GC
Cuts at: 292 292
Size: 4616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 734 768 734
Size: 34 4582
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3275 4616 2514
Size: 761 1341 2514
Fragments arranged by size:
2514 1341 761
BclI T'GATC_A
Cuts at: 2136 2136
Size: 4616
BglI GCCn_nnn'nGGC
Cuts at: 485 485
Size: 4616
BglII A'GATC_T
Cuts at: 1178 2131 4168 1178
Size: 953 2037 1626
Fragments arranged by size:
2037 1626 953
BlpI GC'TnA_GC
Cuts at: 1728 1728
Size: 4616
Bme1580I G_kGCm'C
Cuts at: 577 612 1357 2241 2334 3390 577
Size: 35 745 884 93 1056 1803
Fragments arranged by size:
1803 1056 884 745 93 35
BmgBI CAC'GTC
Cuts at: 630 723 1458 630
Size: 93 735 3788
Fragments arranged by size:
3788 735 93
BmrI ACTGGGnnnn_n'
Cuts at: 425 1828 1909 2244 4388 425
Size: 1403 81 335 2144 653
Fragments arranged by size:
2144 1403 653 335 81
BmtI G_CTAG'C
Cuts at: 122 122
Size: 4616
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 583 883 952 1099 163
Size: 420 300 69 147 3680
Fragments arranged by size:
3680 420 300 147 69
Bpu10I CC'TnA_GC
Cuts at: 292 589 292
Size: 297 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 1792 2671 3163 3461 1081
Size: 711 879 492 298 2236
Fragments arranged by size:
2236 879 711 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 878 4320 878
Size: 3442 1174
BsaAI yAC'GTr
Cuts at: 1434 1560 1969 1434
Size: 126 409 4081
Fragments arranged by size:
4081 409 126
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 1364 1442 2047 2295 2997 4493 1364
Size: 78 605 248 702 1496 1487
Fragments arranged by size:
1496 1487 702 605 248 78
BsaWI w'CCGG_w
Cuts at: 260 1805 2326 3004 3278 3425 3836 260
Size: 1545 521 678 274 147 411 1040
Fragments arranged by size:
1545 1040 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 556 586 614 644 193
Size: 30 333 30 28 30 4165
Fragments arranged by size:
4165 333 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1098 1098
Size: 4616
BseYI C'CCAG_C
Cuts at: 1242 2041 2799 3376 3872 1242
Size: 799 758 577 496 1986
Fragments arranged by size:
1986 799 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1090 838
Size: 252 4364
BsiEI CG_ry'CG
Cuts at: 104 422 2158 2204 3412 104
Size: 318 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 318 46
BsiHKAI G_wGCw'C
Cuts at: 211 569 812 836 856 911 1433 1630 2408
Size: 358 243 24 20 55 522 197 778
Cuts at: 2408 2598 3390 211
Size: 190 792 1437
Fragments arranged by size:
1437 792 778 522 358 243 197 190
55 24 20
BsmBI CGTCTCn'nnnn_
Cuts at: 775 1536 775
Size: 761 3855
Bsp1286I G_dGCh'C
Cuts at: 211 569 577 612 812 836 856 911 1357
Size: 358 8 35 200 24 20 55 446
Cuts at: 1357 1433 1610 1630 2241 2334 2408 2598 2660
Size: 76 177 20 611 93 74 190 62
Cuts at: 2660 3390 211
Size: 730 1437
Fragments arranged by size:
1437 730 611 446 358 200 190 177
93 76 74 62 55 35 24 20
20 8
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 4616
BspHI T'CATG_A
Cuts at: 3792 3792
Size: 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1250 1666 1880 2182 2563 1250
Size: 416 214 302 381 3303
Fragments arranged by size:
3303 416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 498 2908 2962 4446 4611 50
Size: 448 2410 54 1484 165 55
Fragments arranged by size:
2410 1484 448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 600 2528 600
Size: 1928 2688
BsrFI r'CCGG_y
Cuts at: 260 417 596 1019 1028 2614 2795 3004 260
Size: 157 179 423 9 1586 181 209 1872
Fragments arranged by size:
1872 1586 423 209 181 179 157 9
BssSI C'ACGA_G
Cuts at: 328 568 643 811 2887 3245 328
Size: 240 75 168 2076 358 1699
Fragments arranged by size:
2076 1699 358 240 168 75
BstAPI GCAn_nnn'nTGC
Cuts at: 816 825 1993 816
Size: 9 1168 3439
Fragments arranged by size:
3439 1168 9
BstBI TT'CG_AA
Cuts at: 2977 2977
Size: 4616
BstEII G'GTnAC_C
Cuts at: 1395 1395
Size: 4616
BstYI r'GATC_y
Cuts at: 1 695 1178 1641 2108 2131 2466 2712 3724
Size: 694 483 463 467 23 335 246 1012
Cuts at: 3724 3810 3822 4168 1
Size: 86 12 346 449
Fragments arranged by size:
1012 694 483 467 463 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4616
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1123 1485 2566 2747 2910 1123
Size: 362 1081 181 163 2829
Fragments arranged by size:
2829 1081 362 181 163
BtsI GCAGTG_nn'
Cuts at: 256 826 1859 256
Size: 570 1033 3013
Fragments arranged by size:
3013 1033 570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 714 749 714
Size: 35 4581
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DraIII CAC_nnn'GTG
Cuts at: 816 816
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 2322 3180 2322
Size: 858 3758
EaeI y'GGCC_r
Cuts at: 255 281 419 839 944 995 1218 2201 2375
Size: 26 138 420 105 51 223 983 174
Cuts at: 2375 2766 2793 4138 255
Size: 391 27 1345 733
Fragments arranged by size:
1345 983 733 420 391 223 174 138
105 51 27 26
EagI C'GGCC_G
Cuts at: 419 2201 419
Size: 1782 2834
EarI CTCTTCn'nnn_
Cuts at: 1339 1525 2639 2849 1339
Size: 186 1114 210 3106
Fragments arranged by size:
3106 1114 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 526 2824 3134 3280 4426 201
Size: 325 2298 310 146 1146 391
Fragments arranged by size:
2298 1146 391 325 310 146
EcoICRI GAG'CTC
Cuts at: 854 909 1628 854
Size: 55 719 3842
Fragments arranged by size:
3842 719 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 583 883 952 1099 1431 2441 2873 3620
Size: 420 300 69 147 332 1010 432 747
Cuts at: 3620 3888 163
Size: 268 891
Fragments arranged by size:
1010 891 747 432 420 332 300 268
147 69
EcoO109I rG'GnC_Cy
Cuts at: 236 455 1101 1353 1755 236
Size: 219 646 252 402 3097
Fragments arranged by size:
3097 646 402 252 219
EcoRI G'AATT_C
Cuts at: 1551 1620 1551
Size: 69 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 681 894 1416 2635 681
Size: 213 522 1219 2662
Fragments arranged by size:
2662 1219 522 213
FspI TGC'GCA
Cuts at: 2397 2397
Size: 4616
HaeII r_GCGC'y
Cuts at: 558 1203 2050 2298 3320 4299 558
Size: 645 847 248 1022 979 875
Fragments arranged by size:
1022 979 875 847 645 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 498 530 2458 2488 2490 2520 498
Size: 32 1928 30 2 30 2594
Fragments arranged by size:
2594 1928 32 30 30 2
HincII GTy'rAC
Cuts at: 1488 1655 1488
Size: 167 4449
KasI G'GCGC_C
Cuts at: 2046 2294 2046
Size: 248 4368
KpnI G_GTAC'C
Cuts at: 1636 1636
Size: 4616
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3287 3471 3287
Size: 184 4432
MscI TGG'CCA
Cuts at: 257 283 841 997 2377 257
Size: 26 558 156 1380 2496
Fragments arranged by size:
2496 1380 558 156 26
MslI CAynn'nnrTG
Cuts at: 2605 2732 2605
Size: 127 4489
MspA1I CmG'CkG
Cuts at: 491 1077 1727 2041 2401 3414 3659 491
Size: 586 650 314 360 1013 245 1448
Fragments arranged by size:
1448 1013 650 586 360 314 245
NaeI GCC'GGC
Cuts at: 419 598 1021 2797 419
Size: 179 423 1776 2238
Fragments arranged by size:
2238 1776 423 179
NarI GG'CG_CC
Cuts at: 2047 2295 2047
Size: 248 4368
NcoI C'CATG_G
Cuts at: 107 107
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 417 596 1019 2795 417
Size: 179 423 1776 2238
Fragments arranged by size:
2238 1776 423 179
NheI G'CTAG_C
Cuts at: 118 118
Size: 4616
NspI r_CATG'y
Cuts at: 1669 2169 3076 1669
Size: 500 907 3209
Fragments arranged by size:
3209 907 500
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PmlI CAC'GTG
Cuts at: 1434 1434
Size: 4616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4584
PpuMI rG'GwC_Cy
Cuts at: 455 1101 455
Size: 646 3970
PspOMI G'GGCC_C
Cuts at: 1353 1353
Size: 4616
PstI C_TGCA'G
Cuts at: 117 220 917 1018 1663 2348 117
Size: 103 697 101 645 685 2385
Fragments arranged by size:
2385 697 685 645 103 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 1077 2041 2401 1077
Size: 964 360 3292
Fragments arranged by size:
3292 964 360
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 856 911 1630 856
Size: 55 719 3842
Fragments arranged by size:
3842 719 55
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SanDI GG'GwC_CC
Cuts at: 455 455
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4406
SbfI CC_TGCA'GG
Cuts at: 1663 1663
Size: 4616
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SfcI C'TryA_G
Cuts at: 33 113 151 216 913 1014 1659 2344 3337
Size: 80 38 65 697 101 645 685 993
Cuts at: 3337 3528 33
Size: 191 1121
Fragments arranged by size:
1121 993 697 685 645 191 101 80
65 38
SfoI GGC'GCC
Cuts at: 2048 2296 2048
Size: 248 4368
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 703 1638 703
Size: 935 3681
SmlI C'TyrA_G
Cuts at: 778 1096 1771 2686 3178 3440 778
Size: 318 675 915 492 262 1954
Fragments arranged by size:
1954 915 675 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 1669 2169 1669
Size: 500 4116
StuI AGG'CCT
Cuts at: 530 1012 530
Size: 482 4134
StyI C'CwwG_G
Cuts at: 107 540 1750 2104 107
Size: 433 1210 354 2619
Fragments arranged by size:
2619 1210 433 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 2199 2332 2791 3000 523
Size: 1676 133 459 209 2139
Fragments arranged by size:
2139 1676 459 209 133
TatI w'GTAC_w
Cuts at: 4131 4131
Size: 4616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 556 588 614 646 4133 4165 556
Size: 32 26 32 3487 32 1007
Fragments arranged by size:
3487 1007 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 721 2413 721
Size: 1692 2924
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 250 2111 174
Size: 76 1861 2679
Fragments arranged by size:
2679 1861 76
XhoI C'TCGA_G
Cuts at: 778 778
Size: 4616
XmaI C'CCGG_G
Cuts at: 701 1636 701
Size: 935 3681
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BfrBI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI