Restriction map for
pF1KE1047
(Circular) (Six-base) MAPSORT of: pF1KE1047.seq Check: 9115 from: 1 to: 4151
pF1KE1047 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1082 1082
Size: 4151
AccI GT'mk_AC
Cuts at: 521 1189 521
Size: 668 3483
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1976 2408 3155 3423 601
Size: 1375 432 747 268 1329
Fragments arranged by size:
1375 1329 747 432 268
AflIII A'CryG_T
Cuts at: 942 1503 2607 3584 942
Size: 561 1104 977 1509
Fragments arranged by size:
1509 1104 977 561
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 351 819 984 1456 3023 351
Size: 468 165 472 1567 1479
Fragments arranged by size:
1567 1479 472 468 165
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 772 1155 3504 3760 772
Size: 383 2349 256 1163
Fragments arranged by size:
2349 1163 383 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 238 716 1171 238
Size: 478 455 3218
Fragments arranged by size:
3218 478 455
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 862 1165 2195 862
Size: 303 1030 2818
Fragments arranged by size:
2818 1030 303
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 451 542 451
Size: 91 4060
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 799 799
Size: 4151
BglII A'GATC_T
Cuts at: 641 1666 3703 641
Size: 1025 2037 1089
Fragments arranged by size:
2037 1089 1025
BlpI GC'TnA_GC
Cuts at: 841 1263 841
Size: 422 3729
Bme1580I G_kGCm'C
Cuts at: 1776 1869 2925 1776
Size: 93 1056 3002
Fragments arranged by size:
3002 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1131 1363 1444 1779 3923 1131
Size: 232 81 335 2144 1359
Fragments arranged by size:
2144 1359 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 526 533 216
Size: 310 7 3834
Fragments arranged by size:
3834 310 7
Bpu10I CC'TnA_GC
Cuts at: 352 352
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2206 2698 2996 1327
Size: 879 492 298 2482
Fragments arranged by size:
2482 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 657 1504 657
Size: 847 3304
BsaBI GATnn'nnATC
Cuts at: 6 116 646 1665 3702 6
Size: 110 530 1019 2037 455
Fragments arranged by size:
2037 1019 530 455 110
BsaHI Gr'CG_yC
Cuts at: 539 1582 1830 2532 4028 539
Size: 1043 248 702 1496 662
Fragments arranged by size:
1496 1043 702 662 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 913 1074 913
Size: 161 3990
BseYI C'CCAG_C
Cuts at: 449 575 924 951 1038 1576 2334 2911 3407
Size: 126 349 27 87 538 758 577 496
Cuts at: 3407 449
Size: 1193
Fragments arranged by size:
1193 758 577 538 496 349 126 87
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 499 513 295
Size: 204 14 3933
Fragments arranged by size:
3933 204 14
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 656 1061 1165 1943 2133 2925 656
Size: 405 104 778 190 792 1882
Fragments arranged by size:
1882 792 778 405 190 104
Bsp1286I G_dGCh'C
Cuts at: 656 862 1061 1165 1776 1869 1943 2133 2195
Size: 206 199 104 611 93 74 190 62
Cuts at: 2195 2925 656
Size: 730 1882
Fragments arranged by size:
1882 730 611 206 199 190 104 93
74 62
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1082 1201 1415 1717 2098 1082
Size: 119 214 302 381 3135
Fragments arranged by size:
3135 381 302 214 119
BsrBI CCG'CTC
Cuts at: 50 327 2443 2497 3981 4146 50
Size: 277 2116 54 1484 165 55
Fragments arranged by size:
2116 1484 277 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 1004 2149 2330 2539 1004
Size: 1145 181 209 2616
Fragments arranged by size:
2616 1145 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4151
BstYI r'GATC_y
Cuts at: 1 117 641 1176 1643 1666 2001 2247 3259
Size: 116 524 535 467 23 335 246 1012
Cuts at: 3259 3345 3357 3703 1
Size: 86 12 346 449
Fragments arranged by size:
1012 535 524 467 449 346 335 246
116 86 23 12
BtgI C'CryG_G
Cuts at: 107 387 487 583 893 107
Size: 280 100 96 310 3365
Fragments arranged by size:
3365 310 280 100 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 301 852 1394 301
Size: 551 542 3058
Fragments arranged by size:
3058 551 542
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 422 659 1736 1910 2301 2328 3673 422
Size: 237 1077 174 391 27 1345 900
Fragments arranged by size:
1345 1077 900 391 237 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 208 1013 2174 2384 208
Size: 805 1161 210 1975
Fragments arranged by size:
1975 1161 805 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 780 2359 2669 2815 3961 780
Size: 1579 310 146 1146 970
Fragments arranged by size:
1579 1146 970 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 526 533 601 1976 2408 3155 3423 216
Size: 310 7 68 1375 432 747 268 944
Fragments arranged by size:
1375 944 747 432 310 268 68 7
EcoO109I rG'GnC_Cy
Cuts at: 699 1133 1290 699
Size: 434 157 3560
Fragments arranged by size:
3560 434 157
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 592 1932 592
Size: 1340 2811
HaeII r_GCGC'y
Cuts at: 351 460 558 1585 1833 2855 3834 351
Size: 109 98 1027 248 1022 979 668
Fragments arranged by size:
1027 1022 979 668 248 109 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1993 2023 2025 2055 1993
Size: 30 2 30 4089
Fragments arranged by size:
4089 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2822 3006 659
Size: 2163 184 1804
Fragments arranged by size:
2163 1804 184
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 2140 2267 2140
Size: 127 4024
MspA1I CmG'CkG
Cuts at: 309 389 453 489 585 767 924 1262 1576
Size: 80 64 36 96 182 157 338 314
Cuts at: 1576 1936 2949 3194 309
Size: 360 1013 245 1266
Fragments arranged by size:
1266 1013 360 338 314 245 182 157
96 80 64 36
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 893 107
Size: 786 3365
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PasI CC'CwG_GG
Cuts at: 797 819 797
Size: 22 4129
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 958 984 958
Size: 26 4125
PfoI T'CCnGG_A
Cuts at: 603 603
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PmlI CAC'GTG
Cuts at: 657 657
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 699 1133 699
Size: 434 3717
PstI C_TGCA'G
Cuts at: 308 464 563 815 1198 1883 308
Size: 156 99 252 383 685 2576
Fragments arranged by size:
2576 685 383 252 156 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 309 453 1576 1936 309
Size: 144 1123 360 2524
Fragments arranged by size:
2524 1123 360 144
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SacII CC_GC'GG
Cuts at: 390 490 586 390
Size: 100 96 3955
Fragments arranged by size:
3955 100 96
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SanDI GG'GwC_CC
Cuts at: 1133 1133
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SexAI A'CCwGG_T
Cuts at: 878 878
Size: 4151
SfcI C'TryA_G
Cuts at: 33 304 460 559 811 1194 1879 2872 3063
Size: 271 156 99 252 383 685 993 191
Cuts at: 3063 33
Size: 1121
Fragments arranged by size:
1121 993 685 383 271 252 191 156
99
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 1306 2221 2713 2975 1306
Size: 915 492 262 2482
Fragments arranged by size:
2482 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StuI AGG'CCT
Cuts at: 503 787 503
Size: 284 3867
StyI C'CwwG_G
Cuts at: 107 893 957 1285 1639 107
Size: 786 64 328 354 2619
Fragments arranged by size:
2619 786 354 328 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 585 617 3668 3700 585
Size: 32 3051 32 1036
Fragments arranged by size:
3051 1036 32 32
Tth111I GACn'n_nGTC
Cuts at: 1137 1948 1137
Size: 811 3340
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspOMI PspXI PsrI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI