Restriction map for
pF1KE1049
(Circular) (Six-base) MAPSORT of: pF1KE1049.seq Check: 6303 from: 1 to: 3788
pF1KE1049 3788 bp
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 389 826 389
Size: 437 3351
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
AfeI AGC'GCT
Cuts at: 238 238
Size: 3788
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 163 383 808 163
Size: 220 425 3143
Fragments arranged by size:
3143 425 220
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 804 1218 1466 1501 804
Size: 414 248 35 3091
Fragments arranged by size:
3091 414 248 35
BanII G_rGCy'C
Cuts at: 802 1832 802
Size: 1030 2758
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 321 768 1000 1081 1416 3560 321
Size: 447 232 81 335 2144 549
Fragments arranged by size:
2144 549 447 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 774 964 1843 2335 2633 283
Size: 491 190 879 492 298 1438
Fragments arranged by size:
1438 879 492 491 298 190
BsaI GGTCTCn'nnnn_
Cuts at: 160 742 3492 160
Size: 582 2750 456
Fragments arranged by size:
2750 582 456
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 134
Size: 3788
BseYI C'CCAG_C
Cuts at: 156 260 1213 1971 2548 3044 156
Size: 104 953 758 577 496 900
Fragments arranged by size:
953 900 758 577 496 104
BsiEI CG_ry'CG
Cuts at: 104 149 1330 1376 2584 104
Size: 45 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46 45
BsiHKAI G_wGCw'C
Cuts at: 179 802 1580 1770 2562 179
Size: 623 778 190 792 1405
Fragments arranged by size:
1405 792 778 623 190
Bsp1286I G_dGCh'C
Cuts at: 179 802 1413 1506 1580 1770 1832 2562 179
Size: 623 611 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 623 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 285 285
Size: 3788
BstYI r'GATC_y
Cuts at: 1 813 1280 1303 1638 1884 2896 2982 2994
Size: 812 467 23 335 246 1012 86 12
Cuts at: 2994 3340 1
Size: 346 449
Fragments arranged by size:
1012 812 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 390 390
Size: 3788
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 1738 1919 2082 336
Size: 1402 181 163 2042
Fragments arranged by size:
2042 1402 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 146 1373 1547 1938 1965 3310 146
Size: 1227 174 391 27 1345 624
Fragments arranged by size:
1345 1227 624 391 174 27
EagI C'GGCC_G
Cuts at: 146 1373 146
Size: 1227 2561
EarI CTCTTCn'nnn_
Cuts at: 187 259 1811 2021 187
Size: 72 1552 210 1954
Fragments arranged by size:
1954 1552 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 131 1996 2306 2452 3598 131
Size: 1865 310 146 1146 321
Fragments arranged by size:
1865 1146 321 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 184 184
Size: 3788
EcoO109I rG'GnC_Cy
Cuts at: 129 303 927 129
Size: 174 624 2990
Fragments arranged by size:
2990 624 174
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 511 1807 511
Size: 1296 2492
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 163 240 1222 1470 2492 3471 163
Size: 77 982 248 1022 979 480
Fragments arranged by size:
1022 982 979 480 248 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 754 786 1630 1660 1662 1692 754
Size: 32 844 30 2 30 2850
Fragments arranged by size:
2850 844 32 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
HindIII A'AGCT_T
Cuts at: 699 699
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 283 1777 1904 283
Size: 1494 127 2167
Fragments arranged by size:
2167 1494 127
MspA1I CmG'CkG
Cuts at: 342 899 1213 1573 2586 2831 342
Size: 557 314 360 1013 245 1299
Fragments arranged by size:
1299 1013 557 360 314 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NotI GC'GGCC_GC
Cuts at: 146 146
Size: 3788
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PpuMI rG'GwC_Cy
Cuts at: 129 303 129
Size: 174 3614
PstI C_TGCA'G
Cuts at: 341 835 1520 341
Size: 494 685 2609
Fragments arranged by size:
2609 685 494
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 342 1213 1573 342
Size: 871 360 2557
Fragments arranged by size:
2557 871 360
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SanDI GG'GwC_CC
Cuts at: 129 129
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 259 1811 2021 259
Size: 1552 210 2026
Fragments arranged by size:
2026 1552 210
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 337 831 1516 2509 2700 33
Size: 304 494 685 993 191 1121
Fragments arranged by size:
1121 993 685 494 304 191
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 298 753 943 1858 2350 2612 298
Size: 455 190 915 492 262 1474
Fragments arranged by size:
1474 915 492 455 262 190
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 107 922 1276 107
Size: 815 354 2619
Fragments arranged by size:
2619 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 1283 282
Size: 1001 2787
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI