Restriction map for
pF1KE1055
(Circular) (Six-base) MAPSORT of: pF1KE1055.seq Check: 4268 from: 1 to: 7853
pF1KE1055 7853 bp
With 182 enzymes: SgfI *
October 31, 2008 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1643 2566 3089 1643
Size: 923 523 6407
Fragments arranged by size:
6407 923 523
AatII G_ACGT'C
Cuts at: 1045 2621 1045
Size: 1576 6277
AccI GT'mk_AC
Cuts at: 4891 4891
Size: 7853
Acc65I G'GTAC_C
Cuts at: 4070 4869 4070
Size: 799 7054
AclI AA'CG_TT
Cuts at: 7693 7693
Size: 7853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 1009 1076 1550 2986 3162 3229 3970 4440
Size: 349 67 474 1436 176 67 741 470
Cuts at: 4440 5678 6110 6857 7125 660
Size: 1238 432 747 268 1388
Fragments arranged by size:
1436 1388 1238 747 741 474 470 432
349 268 176 67 67
AfeI AGC'GCT
Cuts at: 4754 4754
Size: 7853
AflIII A'CryG_T
Cuts at: 393 1986 5205 6309 7286 393
Size: 1593 3219 1104 977 960
Fragments arranged by size:
3219 1593 1104 977 960
AgeI A'CCGG_T
Cuts at: 6241 6241
Size: 7853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3256 3554 3979 4169 3256
Size: 298 425 190 6940
Fragments arranged by size:
6940 425 298 190
AlwNI CAG_nnn'CTG
Cuts at: 3194 5158 6725 3194
Size: 1964 1567 4322
Fragments arranged by size:
4322 1964 1567
ApaI G_GGCC'C
Cuts at: 2576 3485 4674 2576
Size: 909 1189 5755
Fragments arranged by size:
5755 1189 909
ApaLI G'TGCA_C
Cuts at: 1600 3905 6623 1600
Size: 2305 2718 2830
Fragments arranged by size:
2830 2718 2305
ApoI r'AATT_y
Cuts at: 159 3408 3987 4828 4857 7206 7462 159
Size: 3249 579 841 29 2349 256 550
Fragments arranged by size:
3249 2349 841 579 550 256 29
AseI AT'TA_AT
Cuts at: 20 698 20
Size: 678 7175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7853
AvaI C'yCGr_G
Cuts at: 1451 1779 2064 2290 2589 2986 2998 3033 3262
Size: 328 285 226 299 397 12 35 229
Cuts at: 3262 3502 3544 3559 3754 4110 4730 4873 1451
Size: 240 42 15 195 356 620 143 4431
Fragments arranged by size:
4431 620 397 356 328 299 285 240
229 226 195 143 42 35 15 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4054 4087 4054
Size: 33 7820
BamHI G'GATC_C
Cuts at: 1292 4878 1292
Size: 3586 4267
BanI G'GyrC_C
Cuts at: 3074 3820 3916 3925 4070 4286 4869 5283 5531
Size: 746 96 9 145 216 583 414 248
Cuts at: 5531 5566 3074
Size: 35 5361
Fragments arranged by size:
5361 746 583 414 248 216 145 96
35 9
BanII G_rGCy'C
Cuts at: 311 407 467 778 1452 2124 2291 2576 3060
Size: 96 60 311 674 672 167 285 484
Cuts at: 3060 3485 4209 4674 4681 4867 5897 311
Size: 425 724 465 7 186 1030 2267
Fragments arranged by size:
2267 1030 724 674 672 484 465 425
311 285 186 167 96 60 7
BbeI G_GCGC'C
Cuts at: 3078 4290 5287 5535 3078
Size: 1212 997 248 5396
Fragments arranged by size:
5396 1212 997 248
BbsI GAAGACnn'nnnn_
Cuts at: 1436 1728 1999 2231 2974 1436
Size: 292 271 232 743 6315
Fragments arranged by size:
6315 743 292 271 232
BbvCI CC'TCA_GC
Cuts at: 876 2143 3187 876
Size: 1267 1044 5542
Fragments arranged by size:
5542 1267 1044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2922 2956 2922
Size: 34 7819
BciVI GTATCCnnnnn_n'
Cuts at: 620 5751 6512 7853 620
Size: 5131 761 1341 620
Fragments arranged by size:
5131 1341 761 620
BclI T'GATC_A
Cuts at: 362 5373 362
Size: 5011 2842
BfrBI ATG'CAT
Cuts at: 1185 1185
Size: 7853
BglI GCCn_nnn'nGGC
Cuts at: 112 3480 3524 3563 112
Size: 3368 44 39 4402
Fragments arranged by size:
4402 3368 44 39
BglII A'GATC_T
Cuts at: 1810 5368 7405 1810
Size: 3558 2037 2258
Fragments arranged by size:
3558 2258 2037
BlpI GC'TnA_GC
Cuts at: 310 772 2863 4965 310
Size: 462 2091 2102 3198
Fragments arranged by size:
3198 2102 2091 462
Bme1580I G_kGCm'C
Cuts at: 1604 2576 3476 3485 3909 4674 5478 5571 6627
Size: 972 900 9 424 765 804 93 1056
Cuts at: 6627 1604
Size: 2830
Fragments arranged by size:
2830 1056 972 900 804 765 424 93
9
BmgBI CAC'GTC
Cuts at: 3218 3393 3218
Size: 175 7678
BmrI ACTGGGnnnn_n'
Cuts at: 111 1566 1689 4051 5065 5146 5481 7625 111
Size: 1455 123 2362 1014 81 335 2144 339
Fragments arranged by size:
2362 2144 1455 1014 339 335 123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 372 6220 372
Size: 5848 2005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 930 1841 2161 2706 2718 2808 3005 3352
Size: 732 911 320 545 12 90 197 347
Cuts at: 3352 4638 198
Size: 1286 3413
Fragments arranged by size:
3413 1286 911 732 545 347 320 197
90 12
Bpu10I CC'TnA_GC
Cuts at: 876 1198 1215 2143 2683 3187 4675 876
Size: 322 17 928 540 504 1488 4054
Fragments arranged by size:
4054 1488 928 540 504 322 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1164 1653 1827 2878 3622 3741 5029 5908 6400
Size: 489 174 1051 744 119 1288 879 492
Cuts at: 6400 6698 1164
Size: 298 2319
Fragments arranged by size:
2319 1288 1051 879 744 492 489 298
174 119
BsaI GGTCTCn'nnnn_
Cuts at: 2318 2523 7557 2318
Size: 205 5034 2614
Fragments arranged by size:
5034 2614 205
BsaAI yAC'GTr
Cuts at: 396 608 2439 5206 396
Size: 212 1831 2767 3043
Fragments arranged by size:
3043 2767 1831 212
BsaBI GATnn'nnATC
Cuts at: 6 5367 7404 6
Size: 5361 2037 455
Fragments arranged by size:
5361 2037 455
BsaHI Gr'CG_yC
Cuts at: 1042 2618 2743 2810 2935 3075 4287 4499 4735
Size: 1576 125 67 125 140 1212 212 236
Cuts at: 4735 5284 5532 6234 7730 1042
Size: 549 248 702 1496 1165
Fragments arranged by size:
1576 1496 1212 1165 702 549 248 236
212 140 125 125 67
BsaWI w'CCGG_w
Cuts at: 612 644 1979 3607 5042 5563 6241 6515 6662
Size: 32 1335 1628 1435 521 678 274 147
Cuts at: 6662 7073 612
Size: 411 1392
Fragments arranged by size:
1628 1435 1392 1335 678 521 411 274
147 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 1633 1663 1957 1987 2785 2815 3610
Size: 30 1500 30 294 30 798 30 795
Cuts at: 3610 3640 103
Size: 30 4316
Fragments arranged by size:
4316 1500 798 795 294 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 470 2513 2688 3051 3129 3618 3738 4078 4701
Size: 2043 175 363 78 489 120 340 623
Cuts at: 4701 470
Size: 3622
Fragments arranged by size:
3622 2043 623 489 363 340 175 120
78
BseYI C'CCAG_C
Cuts at: 807 1438 2854 2879 3307 3445 3736 4749 5278
Size: 631 1416 25 428 138 291 1013 529
Cuts at: 5278 6036 6613 7109 807
Size: 758 577 496 1551
Fragments arranged by size:
1551 1416 1013 758 631 577 529 496
428 291 138 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1671 1932 3063 3418 1671
Size: 261 1131 355 6106
Fragments arranged by size:
6106 1131 355 261
BsiEI CG_ry'CG
Cuts at: 104 2056 2110 2764 5395 5441 6649 104
Size: 1952 54 654 2631 46 1208 1308
Fragments arranged by size:
2631 1952 1308 1208 654 54 46
BsiHKAI G_wGCw'C
Cuts at: 178 1604 1847 2438 3791 3909 3958 4408 4768
Size: 1426 243 591 1353 118 49 450 360
Cuts at: 4768 4867 5645 5835 6627 178
Size: 99 778 190 792 1404
Fragments arranged by size:
1426 1404 1353 792 778 591 450 360
243 190 118 99 49
BsmI GAATG_Cn'
Cuts at: 1908 2176 1908
Size: 268 7585
BsmBI CGTCTCn'nnnn_
Cuts at: 1049 1176 1049
Size: 127 7726
Bsp1286I G_dGCh'C
Cuts at: 178 311 407 467 778 1452 1604 1847 2124
Size: 133 96 60 311 674 152 243 277
Cuts at: 2124 2291 2438 2576 3060 3476 3485 3791 3909
Size: 167 147 138 484 416 9 306 118
Cuts at: 3909 3958 4209 4408 4674 4681 4768 4867 5478
Size: 49 251 199 266 7 87 99 611
Cuts at: 5478 5571 5645 5835 5897 6627 178
Size: 93 74 190 62 730 1404
Fragments arranged by size:
1404 730 674 611 484 416 311 306
277 266 251 243 199 190 167 152
147 138 133 118 99 96 93 87
74 62 60 49 9 7
BspEI T'CCGG_A
Cuts at: 612 1979 3607 7073 612
Size: 1367 1628 3466 1392
Fragments arranged by size:
3466 1628 1392 1367
BspHI T'CATG_A
Cuts at: 531 7029 531
Size: 6498 1355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 626 1643 2566 3089 3587 4360 4903 5117 5419
Size: 1017 923 523 498 773 543 214 302
Cuts at: 5419 5800 626
Size: 381 2679
Fragments arranged by size:
2679 1017 923 773 543 523 498 381
302 214
BsrBI CCG'CTC
Cuts at: 50 722 2696 2732 2822 3728 3974 6145 6199
Size: 672 1974 36 90 906 246 2171 54
Cuts at: 6199 7683 7848 50
Size: 1484 165 55
Fragments arranged by size:
2171 1974 1484 906 672 246 165 90
55 54 36
BsrDI GCAATG_nn'
Cuts at: 571 5765 571
Size: 5194 2659
BsrFI r'CCGG_y
Cuts at: 889 2759 3999 5851 6032 6241 889
Size: 1870 1240 1852 181 209 2501
Fragments arranged by size:
2501 1870 1852 1240 209 181
BssSI C'ACGA_G
Cuts at: 1359 3164 3238 6124 6482 1359
Size: 1805 74 2886 358 2730
Fragments arranged by size:
2886 2730 1805 358 74
BstAPI GCAn_nnn'nTGC
Cuts at: 1599 3795 5230 1599
Size: 2196 1435 4222
Fragments arranged by size:
4222 2196 1435
BstBI TT'CG_AA
Cuts at: 6214 6214
Size: 7853
BstEII G'GTnAC_C
Cuts at: 1387 1387
Size: 7853
BstXI CCAn_nnnn'nTGG
Cuts at: 909 1056 1627 2430 4776 909
Size: 147 571 803 2346 3986
Fragments arranged by size:
3986 2346 803 571 147
BstYI r'GATC_y
Cuts at: 1 374 632 1193 1292 1810 2705 4878 5345
Size: 373 258 561 99 518 895 2173 467
Cuts at: 5345 5368 5703 5949 6961 7047 7059 7405 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2173 1012 895 561 518 467 449 373
346 335 258 246 99 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2700 2700
Size: 7853
BtgI C'CryG_G
Cuts at: 107 378 420 437 765 3060 3105 3221 3344
Size: 271 42 17 328 2295 45 116 123
Cuts at: 3344 3477 3649 4448 107
Size: 133 172 799 3512
Fragments arranged by size:
3512 2295 799 328 271 172 133 123
116 45 42 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 725 3223 5803 5984 6147 725
Size: 2498 2580 181 163 2431
Fragments arranged by size:
2580 2498 2431 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 2372 5096 1379
Size: 993 2724 4136
Fragments arranged by size:
4136 2724 993
ClaI AT'CG_AT
Cuts at: 2339 2339
Size: 7853
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2370 2405 4559 4594 2370
Size: 35 2154 35 5629
Fragments arranged by size:
5629 2154 35 35
DraI TTT'AAA
Cuts at: 3407 4852 3407
Size: 1445 6408
DraIII CAC_nnn'GTG
Cuts at: 3111 3111
Size: 7853
DrdI GACnn_nn'nnGTC
Cuts at: 5559 6417 5559
Size: 858 6995
EaeI y'GGCC_r
Cuts at: 843 2053 2103 2761 2780 3212 3270 3525 5438
Size: 1210 50 658 19 432 58 255 1913
Cuts at: 5438 5612 6003 6030 7375 843
Size: 174 391 27 1345 1321
Fragments arranged by size:
1913 1345 1321 1210 658 432 391 255
174 58 50 27 19
EagI C'GGCC_G
Cuts at: 2053 2761 5438 2053
Size: 708 2677 4468
Fragments arranged by size:
4468 2677 708
EarI CTCTTCn'nnn_
Cuts at: 1095 1164 3946 5876 6086 1095
Size: 69 2782 1930 210 2862
Fragments arranged by size:
2862 2782 1930 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 415 576 6061 6371 6517 7663 415
Size: 161 5485 310 146 1146 605
Fragments arranged by size:
5485 1146 605 310 161 146
EcoICRI GAG'CTC
Cuts at: 4865 4865
Size: 7853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 660 930 1009 1076 1550 1841 2161 2706
Size: 462 270 79 67 474 291 320 545
Cuts at: 2706 2718 2808 2986 3005 3162 3229 3352 3970
Size: 12 90 178 19 157 67 123 618
Cuts at: 3970 4440 4638 5678 6110 6857 7125 198
Size: 470 198 1040 432 747 268 926
Fragments arranged by size:
1040 926 747 618 545 474 470 462
432 320 291 270 268 198 178 157
123 90 79 67 67 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 627 1286 1489 2528 4513 627
Size: 659 203 1039 1985 3967
Fragments arranged by size:
3967 1985 1039 659 203
EcoO109I rG'GnC_Cy
Cuts at: 353 383 2139 3150 3858 4053 4670 4671 4992
Size: 30 1756 1011 708 195 617 1 321
Cuts at: 4992 353
Size: 3214
Fragments arranged by size:
3214 1756 1011 708 617 321 195 30
1
EcoRI G'AATT_C
Cuts at: 159 4857 159
Size: 4698 3155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 5872 1612
Size: 4260 3593
FseI GG_CCGG'CC
Cuts at: 2763 2763
Size: 7853
FspI TGC'GCA
Cuts at: 5634 5634
Size: 7853
HaeII r_GCGC'y
Cuts at: 2660 2983 3078 3452 3617 4009 4290 4529 4756
Size: 323 95 374 165 392 281 239 227
Cuts at: 4756 5287 5535 6557 7536 2660
Size: 531 248 1022 979 2977
Fragments arranged by size:
2977 1022 979 531 392 374 323 281
248 239 227 165 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 1957 1989 2027 2059 2785 2817 3610
Size: 32 1822 32 38 32 726 32 793
Cuts at: 3610 3642 5695 5725 5727 5757 103
Size: 32 2053 30 2 30 2199
Fragments arranged by size:
2199 2053 1822 793 726 38 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 4892 4892
Size: 7853
HindIII A'AGCT_T
Cuts at: 4233 4233
Size: 7853
KasI G'GCGC_C
Cuts at: 3074 4286 5283 5531 3074
Size: 1212 997 248 5396
Fragments arranged by size:
5396 1212 997 248
KpnI G_GTAC'C
Cuts at: 4074 4873 4074
Size: 799 7054
MfeI C'AATT_G
Cuts at: 515 515
Size: 7853
MluI A'CGCG_T
Cuts at: 7286 7286
Size: 7853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1294 2303 2573 6524 6708 1294
Size: 1009 270 3951 184 2439
Fragments arranged by size:
3951 2439 1009 270 184
MscI TGG'CCA
Cuts at: 845 3214 3527 5614 845
Size: 2369 313 2087 3084
Fragments arranged by size:
3084 2369 2087 313
MslI CAynn'nnrTG
Cuts at: 907 1625 1913 1929 3961 4774 5842 5969 907
Size: 718 288 16 2032 813 1068 127 2791
Fragments arranged by size:
2791 2032 1068 813 718 288 127 16
MspA1I CmG'CkG
Cuts at: 422 655 880 3194 3223 3651 3658 3984 4021
Size: 233 225 2314 29 428 7 326 37
Cuts at: 4021 4401 4505 4964 5278 5638 6651 6896 422
Size: 380 104 459 314 360 1013 245 1379
Fragments arranged by size:
2314 1379 1013 459 428 380 360 326
314 245 233 225 104 37 29 7
NaeI GCC'GGC
Cuts at: 891 2761 6034 891
Size: 1870 3273 2710
Fragments arranged by size:
3273 2710 1870
NarI GG'CG_CC
Cuts at: 3075 4287 5284 5532 3075
Size: 1212 997 248 5396
Fragments arranged by size:
5396 1212 997 248
NcoI C'CATG_G
Cuts at: 107 378 437 3344 107
Size: 271 59 2907 4616
Fragments arranged by size:
4616 2907 271 59
NdeI CA'TA_TG
Cuts at: 578 1715 1926 578
Size: 1137 211 6505
Fragments arranged by size:
6505 1137 211
NgoMIV G'CCGG_C
Cuts at: 889 2759 6032 889
Size: 1870 3273 2710
Fragments arranged by size:
3273 2710 1870
NsiI A_TGCA'T
Cuts at: 1187 1187
Size: 7853
NspI r_CATG'y
Cuts at: 1185 1990 2637 4044 4104 4906 5406 6313 1185
Size: 805 647 1407 60 802 500 907 2725
Fragments arranged by size:
2725 1407 907 805 802 647 500 60
PasI CC'CwG_GG
Cuts at: 1444 1444
Size: 7853
PciI A'CATG_T
Cuts at: 1986 6309 1986
Size: 4323 3530
PflMI CCAn_nnn'nTGG
Cuts at: 2227 3590 4449 4602 2227
Size: 1363 859 153 5478
Fragments arranged by size:
5478 1363 859 153
PfoI T'CCnGG_A
Cuts at: 243 3455 243
Size: 3212 4641
PmeI GTTT'AAAC
Cuts at: 4852 4852
Size: 7853
PmlI CAC'GTG
Cuts at: 396 396
Size: 7853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1203 1235 6992 7024 1203
Size: 32 5757 32 2032
Fragments arranged by size:
5757 2032 32 32
PpuMI rG'GwC_Cy
Cuts at: 383 2139 4053 383
Size: 1756 1914 4183
Fragments arranged by size:
4183 1914 1756
PshAI GACnn'nnGTC
Cuts at: 4343 4343
Size: 7853
PspOMI G'GGCC_C
Cuts at: 2572 3481 4670 2572
Size: 909 1189 5755
Fragments arranged by size:
5755 1189 909
PspXI vC'TCGA_Gb
Cuts at: 2290 2998 3544 2290
Size: 708 546 6599
Fragments arranged by size:
6599 708 546
PstI C_TGCA'G
Cuts at: 1374 2563 3086 4322 4357 4418 4900 5585 1374
Size: 1189 523 1236 35 61 482 685 3642
Fragments arranged by size:
3642 1236 1189 685 523 482 61 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7853
PvuII CAG'CTG
Cuts at: 880 3194 3984 4021 4505 5278 5638 880
Size: 2314 790 37 484 773 360 3095
Fragments arranged by size:
3095 2314 790 773 484 360 37
RsrII CG'GwC_CG
Cuts at: 6048 6048
Size: 7853
SacI G_AGCT'C
Cuts at: 4867 4867
Size: 7853
SacII CC_GC'GG
Cuts at: 423 3224 3652 423
Size: 2801 428 4624
Fragments arranged by size:
4624 2801 428
SalI G'TCGA_C
Cuts at: 4890 4890
Size: 7853
SanDI GG'GwC_CC
Cuts at: 383 383
Size: 7853
SapI GCTCTTCn'nnn_
Cuts at: 1095 3946 5876 6086 1095
Size: 2851 1930 210 2862
Fragments arranged by size:
2862 2851 1930 210
SbfI CC_TGCA'GG
Cuts at: 4357 4900 4357
Size: 543 7310
ScaI AGT'ACT
Cuts at: 7370 7370
Size: 7853
SfcI C'TryA_G
Cuts at: 33 779 1370 2559 3082 4318 4353 4414 4513
Size: 746 591 1189 523 1236 35 61 99
Cuts at: 4513 4896 5581 6574 6765 33
Size: 383 685 993 191 1121
Fragments arranged by size:
1236 1189 1121 993 746 685 591 523
383 191 99 61 35
SfoI GGC'GCC
Cuts at: 3076 4288 5285 5533 3076
Size: 1212 997 248 5396
Fragments arranged by size:
5396 1212 997 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7853
SmaI CCC'GGG
Cuts at: 2591 3504 4112 4875 2591
Size: 913 608 763 5569
Fragments arranged by size:
5569 913 763 608
SmlI C'TyrA_G
Cuts at: 1143 1668 1779 1806 2290 2893 2998 3544 3637
Size: 525 111 27 484 603 105 546 93
Cuts at: 3637 3720 5008 5923 6415 6677 1143
Size: 83 1288 915 492 262 2319
Fragments arranged by size:
2319 1288 915 603 546 525 492 484
262 111 105 93 83 27
SnaBI TAC'GTA
Cuts at: 608 608
Size: 7853
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7853
SphI G_CATG'C
Cuts at: 4044 4104 4906 5406 4044
Size: 60 802 500 6491
Fragments arranged by size:
6491 802 500 60
SrfI GCCC'GGGC
Cuts at: 3504 3504
Size: 7853
StuI AGG'CCT
Cuts at: 1281 1774 1281
Size: 493 7360
StyI C'CwwG_G
Cuts at: 107 378 437 1055 2124 3344 4179 4987 5341
Size: 271 59 618 1069 1220 835 808 354
Cuts at: 5341 107
Size: 2619
Fragments arranged by size:
2619 1220 1069 835 808 618 354 271
59
SwaI ATTT'AAAT
Cuts at: 3407 3407
Size: 7853
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1600 2124 2320 2557 5436 5569 6028 6237 1600
Size: 524 196 237 2879 133 459 209 3216
Fragments arranged by size:
3216 2879 524 459 237 209 196 133
TatI w'GTAC_w
Cuts at: 7368 7368
Size: 7853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 284 316 1633 1665 7370 7402 284
Size: 32 1317 32 5705 32 735
Fragments arranged by size:
5705 1317 735 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5650 5650
Size: 7853
XbaI T'CTAG_A
Cuts at: 4884 4884
Size: 7853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5348 5348
Size: 7853
XhoI C'TCGA_G
Cuts at: 1779 2290 2998 3544 1779
Size: 511 708 546 6088
Fragments arranged by size:
6088 708 546 511
XmaI C'CCGG_G
Cuts at: 2589 3502 4110 4873 2589
Size: 913 608 763 5569
Fragments arranged by size:
5569 913 763 608
XmnI GAAnn'nnTTC
Cuts at: 917 1642 1684 1908 917
Size: 725 42 224 6862
Fragments arranged by size:
6862 725 224 42
ZraI GAC'GTC
Cuts at: 1043 2619 1043
Size: 1576 6277
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BmtI BsiWI
BsrGI BssHII BstZ17I EcoRV FspAI HpaI NheI NotI
NruI PacI PsiI PsrI SexAI SfiI SgrAI SspI