Restriction map for
pF1KE1059
(Circular) (Six-base) MAPSORT of: pF1KE1059.seq Check: 2628 from: 1 to: 5723
pF1KE1059 5723 bp
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 833 833
Size: 5723
AccI GT'mk_AC
Cuts at: 449 776 1022 1322 2761 449
Size: 327 246 300 1439 3411
Fragments arranged by size:
3411 1439 327 300 246
Acc65I G'GTAC_C
Cuts at: 2739 2739
Size: 5723
AclI AA'CG_TT
Cuts at: 5563 5563
Size: 5723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1905 2230 3548 3980 4727 4995 1905
Size: 325 1318 432 747 268 2633
Fragments arranged by size:
2633 1318 747 432 325 268
AfeI AGC'GCT
Cuts at: 137 137
Size: 5723
AflIII A'CryG_T
Cuts at: 1187 3075 4179 5156 1187
Size: 1888 1104 977 1754
Fragments arranged by size:
1888 1754 1104 977
AgeI A'CCGG_T
Cuts at: 4111 4111
Size: 5723
AhdI GACnn_n'nnGTC
Cuts at: 2502 2502
Size: 5723
AleI CACnn'nnGTG
Cuts at: 757 757
Size: 5723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1922 1069
Size: 853 4870
AlwNI CAG_nnn'CTG
Cuts at: 225 328 619 1617 1770 2208 2337 3028 4595
Size: 103 291 998 153 438 129 691 1567
Cuts at: 4595 225
Size: 1353
Fragments arranged by size:
1567 1353 998 691 438 291 153 129
103
ApaI G_GGCC'C
Cuts at: 2321 2321
Size: 5723
ApaLI G'TGCA_C
Cuts at: 1759 4493 1759
Size: 2734 2989
ApoI r'AATT_y
Cuts at: 2727 5076 5332 2727
Size: 2349 256 3118
Fragments arranged by size:
3118 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5723
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
AvaI C'yCGr_G
Cuts at: 498 834 1254 1867 1944 2295 2743 498
Size: 336 420 613 77 351 448 3478
Fragments arranged by size:
3478 613 448 420 351 336 77
AvrII C'CTAG_G
Cuts at: 2697 2697
Size: 5723
BamHI G'GATC_C
Cuts at: 2748 2748
Size: 5723
BanI G'GyrC_C
Cuts at: 257 456 606 648 747 1258 1341 1963 2739
Size: 199 150 42 99 511 83 622 776
Cuts at: 2739 3153 3401 3436 257
Size: 414 248 35 2544
Fragments arranged by size:
2544 776 622 511 414 248 199 150
99 83 42 35
BanII G_rGCy'C
Cuts at: 173 2321 2737 3767 173
Size: 2148 416 1030 2129
Fragments arranged by size:
2148 2129 1030 416
BbeI G_GCGC'C
Cuts at: 261 3157 3405 261
Size: 2896 248 2579
Fragments arranged by size:
2896 2579 248
BbsI GAAGACnn'nnnn_
Cuts at: 2143 2143
Size: 5723
BbvCI CC'TCA_GC
Cuts at: 362 2467 362
Size: 2105 3618
BciVI GTATCCnnnnn_n'
Cuts at: 3621 4382 5723 3621
Size: 761 1341 3621
Fragments arranged by size:
3621 1341 761
BclI T'GATC_A
Cuts at: 3243 3243
Size: 5723
BglI GCCn_nnn'nGGC
Cuts at: 230 324 352 615 893 1808 2058 230
Size: 94 28 263 278 915 250 3895
Fragments arranged by size:
3895 915 278 263 250 94 28
BglII A'GATC_T
Cuts at: 290 2435 3238 5275 290
Size: 2145 803 2037 738
Fragments arranged by size:
2145 2037 803 738
BlpI GC'TnA_GC
Cuts at: 476 1729 2835 476
Size: 1253 1106 3364
Fragments arranged by size:
3364 1253 1106
Bme1580I G_kGCm'C
Cuts at: 461 611 724 752 863 977 1058 1097 1232
Size: 150 113 28 111 114 81 39 135
Cuts at: 1232 1261 1277 1716 1763 1868 2321 3348 3441
Size: 29 16 439 47 105 453 1027 93
Cuts at: 3441 4497 461
Size: 1056 1687
Fragments arranged by size:
1687 1056 1027 453 439 150 135 114
113 111 105 93 81 47 39 29
28 16
BmgBI CAC'GTC
Cuts at: 972 1188 1197 1227 1758 2529 972
Size: 216 9 30 531 771 4166
Fragments arranged by size:
4166 771 531 216 30 9
BmrI ACTGGGnnnn_n'
Cuts at: 393 974 1577 2935 3016 3351 5495 393
Size: 581 603 1358 81 335 2144 621
Fragments arranged by size:
2144 1358 621 603 581 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4090 4090
Size: 5723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 641 979 1148 1380 1489 1835 2215 2371
Size: 415 338 169 232 109 346 380 156
Cuts at: 2371 226
Size: 3578
Fragments arranged by size:
3578 415 380 346 338 232 169 156
109
Bpu10I CC'TnA_GC
Cuts at: 362 2467 362
Size: 2105 3618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1846 2899 3778 4270 4568 271
Size: 1575 1053 879 492 298 1426
Fragments arranged by size:
1575 1426 1053 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 941 1075 5427 941
Size: 134 4352 1237
Fragments arranged by size:
4352 1237 134
BsaAI yAC'GTr
Cuts at: 3076 3076
Size: 5723
BsaBI GATnn'nnATC
Cuts at: 6 3237 5274 6
Size: 3231 2037 455
Fragments arranged by size:
3231 2037 455
BsaHI Gr'CG_yC
Cuts at: 258 1874 2275 2396 3154 3402 4104 5600 258
Size: 1616 401 121 758 248 702 1496 381
Fragments arranged by size:
1616 1496 758 702 401 381 248 121
BsaWI w'CCGG_w
Cuts at: 2912 3433 4111 4385 4532 4943 2912
Size: 521 678 274 147 411 3692
Fragments arranged by size:
3692 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1563 1593 1563
Size: 30 5693
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1093 2140 1093
Size: 1047 4676
BseYI C'CCAG_C
Cuts at: 356 381 434 466 505 528 580 713 788
Size: 25 53 32 39 23 52 133 75
Cuts at: 788 883 1350 1368 2664 2678 3148 3906 4483
Size: 95 467 18 1296 14 470 758 577
Cuts at: 4483 4979 356
Size: 496 1100
Fragments arranged by size:
1296 1100 758 577 496 470 467 133
95 75 53 52 39 32 25 23
18 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 1882 1954 1630
Size: 252 72 5399
Fragments arranged by size:
5399 252 72
BsiEI CG_ry'CG
Cuts at: 104 1181 3265 3311 4519 104
Size: 1077 2084 46 1208 1308
Fragments arranged by size:
2084 1308 1208 1077 46
BsiHKAI G_wGCw'C
Cuts at: 173 1763 2737 3515 3705 4497 173
Size: 1590 974 778 190 792 1399
Fragments arranged by size:
1590 1399 974 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 173 461 611 724 752 863 977 1058 1097
Size: 288 150 113 28 111 114 81 39
Cuts at: 1097 1232 1261 1277 1716 1763 1868 2321 2737
Size: 135 29 16 439 47 105 453 416
Cuts at: 2737 3348 3441 3515 3705 3767 4497 173
Size: 611 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 453 439 416 288 190
150 135 114 113 111 105 93 81
74 62 47 39 29 28 16
BspEI T'CCGG_A
Cuts at: 4943 4943
Size: 5723
BspHI T'CATG_A
Cuts at: 194 4899 194
Size: 4705 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 833 1625 2567 2773 2987 3289 3670 833
Size: 792 942 206 214 302 381 2886
Fragments arranged by size:
2886 942 792 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 4015 4069 5553 5718 50
Size: 3965 54 1484 165 55
Fragments arranged by size:
3965 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1695 1911 3635 1695
Size: 216 1724 3783
Fragments arranged by size:
3783 1724 216
BsrFI r'CCGG_y
Cuts at: 1176 1402 1558 1733 1838 2327 3721 3902 4111
Size: 226 156 175 105 489 1394 181 209
Cuts at: 4111 1176
Size: 2788
Fragments arranged by size:
2788 1394 489 226 209 181 175 156
105
BssSI C'ACGA_G
Cuts at: 1654 3994 4352 1654
Size: 2340 358 3025
Fragments arranged by size:
3025 2340 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1617 2337 3100 1617
Size: 720 763 4240
Fragments arranged by size:
4240 763 720
BstBI TT'CG_AA
Cuts at: 4084 4084
Size: 5723
BstEII G'GTnAC_C
Cuts at: 2541 2541
Size: 5723
BstXI CCAn_nnnn'nTGG
Cuts at: 759 1884 2391 759
Size: 1125 507 4091
Fragments arranged by size:
4091 1125 507
BstYI r'GATC_y
Cuts at: 1 290 2435 2748 3215 3238 3573 3819 4831
Size: 289 2145 313 467 23 335 246 1012
Cuts at: 4831 4917 4929 5275 1
Size: 86 12 346 449
Fragments arranged by size:
2145 1012 467 449 346 335 313 289
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 777 777
Size: 5723
Bsu36I CC'TnA_GG
Cuts at: 1298 1952 1298
Size: 654 5069
BtgI C'CryG_G
Cuts at: 698 920 2390 2411 2630 698
Size: 222 1470 21 219 3791
Fragments arranged by size:
3791 1470 222 219 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2606 3673 3854 4017 2606
Size: 1067 181 163 4312
Fragments arranged by size:
4312 1067 181 163
BtsI GCAGTG_nn'
Cuts at: 1066 1912 1937 2362 2966 1066
Size: 846 25 425 604 3823
Fragments arranged by size:
3823 846 604 425 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 725 760 725
Size: 35 5688
DraI TTT'AAA
Cuts at: 2722 2722
Size: 5723
DrdI GACnn_nn'nnGTC
Cuts at: 3429 4287 3429
Size: 858 4865
EaeI y'GGCC_r
Cuts at: 128 152 587 894 942 1178 1631 1800 2264
Size: 24 435 307 48 236 453 169 464
Cuts at: 2264 2414 2588 3308 3482 3873 3900 5245 128
Size: 150 174 720 174 391 27 1345 606
Fragments arranged by size:
1345 720 606 464 453 435 391 307
236 174 174 169 150 48 27 24
EagI C'GGCC_G
Cuts at: 1178 3308 1178
Size: 2130 3593
EarI CTCTTCn'nnn_
Cuts at: 1924 2347 3746 3956 1924
Size: 423 1399 210 3691
Fragments arranged by size:
3691 1399 423 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 3931 4241 4387 5533 2677
Size: 1254 310 146 1146 2867
Fragments arranged by size:
2867 1254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 171 2735 171
Size: 2564 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 641 979 1148 1380 1489 1835 1905 2215
Size: 415 338 169 232 109 346 70 310
Cuts at: 2215 2230 2371 3548 3980 4727 4995 226
Size: 15 141 1177 432 747 268 954
Fragments arranged by size:
1177 954 747 432 415 346 338 310
268 232 169 141 109 70 15
EcoNI CCTnn'n_nnAGG
Cuts at: 601 601
Size: 5723
EcoO109I rG'GnC_Cy
Cuts at: 222 325 502 616 625 687 934 993 1491
Size: 103 177 114 9 62 247 59 498
Cuts at: 1491 1614 1788 1830 1948 2317 2318 2862 222
Size: 123 174 42 118 369 1 544 3083
Fragments arranged by size:
3083 544 498 369 247 177 174 123
118 114 103 62 59 42 9 1
EcoRI G'AATT_C
Cuts at: 2727 2727
Size: 5723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3742 3742
Size: 5723
FspI TGC'GCA
Cuts at: 2533 3504 2533
Size: 971 4752
HaeII r_GCGC'y
Cuts at: 139 261 388 1386 1523 1559 2474 3157 3405
Size: 122 127 998 137 36 915 683 248
Cuts at: 3405 4427 5406 139
Size: 1022 979 456
Fragments arranged by size:
1022 998 979 915 683 456 248 137
127 122 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 552 584 2488 2520 3565 3595 3597
Size: 32 363 32 1904 32 1045 30 2
Cuts at: 3597 3627 157
Size: 30 2253
Fragments arranged by size:
2253 1904 1045 363 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1323 2762 1323
Size: 1439 4284
HindIII A'AGCT_T
Cuts at: 1726 1726
Size: 5723
KasI G'GCGC_C
Cuts at: 257 3153 3401 257
Size: 2896 248 2579
Fragments arranged by size:
2896 2579 248
KpnI G_GTAC'C
Cuts at: 2743 2743
Size: 5723
MluI A'CGCG_T
Cuts at: 5156 5156
Size: 5723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1643 4394 4578 1031
Size: 612 2751 184 2176
Fragments arranged by size:
2751 2176 612 184
MscI TGG'CCA
Cuts at: 130 896 2266 2416 2590 3484 130
Size: 766 1370 150 174 894 2369
Fragments arranged by size:
2369 1370 894 766 174 150
MslI CAynn'nnrTG
Cuts at: 199 409 757 1904 3712 3839 199
Size: 210 348 1147 1808 127 2083
Fragments arranged by size:
2083 1808 1147 348 210 127
MspA1I CmG'CkG
Cuts at: 713 1354 1605 1740 1884 2193 2208 2334 2605
Size: 641 251 135 144 309 15 126 271
Cuts at: 2605 2632 2706 2834 3148 3508 4521 4766 713
Size: 27 74 128 314 360 1013 245 1670
Fragments arranged by size:
1670 1013 641 360 314 309 271 251
245 144 135 128 126 74 27 15
NaeI GCC'GGC
Cuts at: 1178 1735 3904 1178
Size: 557 2169 2997
Fragments arranged by size:
2997 2169 557
NarI GG'CG_CC
Cuts at: 258 3154 3402 258
Size: 2896 248 2579
Fragments arranged by size:
2896 2579 248
NcoI C'CATG_G
Cuts at: 698 920 698
Size: 222 5501
NgoMIV G'CCGG_C
Cuts at: 1176 1733 3902 1176
Size: 557 2169 2997
Fragments arranged by size:
2997 2169 557
NspI r_CATG'y
Cuts at: 306 1723 2776 3276 4183 306
Size: 1417 1053 500 907 1846
Fragments arranged by size:
1846 1417 1053 907 500
PasI CC'CwG_GG
Cuts at: 613 613
Size: 5723
PciI A'CATG_T
Cuts at: 4179 4179
Size: 5723
PflMI CCAn_nnn'nTGG
Cuts at: 619 1539 1770 2262 2289 2481 2643 619
Size: 920 231 492 27 192 162 3699
Fragments arranged by size:
3699 920 492 231 192 162 27
PmeI GTTT'AAAC
Cuts at: 2722 2722
Size: 5723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 4862
Size: 32 5691
PpuMI rG'GwC_Cy
Cuts at: 502 687 934 1614 1830 502
Size: 185 247 680 216 4395
Fragments arranged by size:
4395 680 247 216 185
PspOMI G'GGCC_C
Cuts at: 2317 2317
Size: 5723
PstI C_TGCA'G
Cuts at: 1508 1622 1745 2770 3455 1508
Size: 114 123 1025 685 3776
Fragments arranged by size:
3776 1025 685 123 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5723
PvuII CAG'CTG
Cuts at: 713 1354 1740 1884 2193 2208 2334 2706 3148
Size: 641 386 144 309 15 126 372 442
Cuts at: 3148 3508 713
Size: 360 2928
Fragments arranged by size:
2928 641 442 386 372 360 309 144
126 15
RsrII CG'GwC_CG
Cuts at: 2325 3918 2325
Size: 1593 4130
SacI G_AGCT'C
Cuts at: 173 2737 173
Size: 2564 3159
SacII CC_GC'GG
Cuts at: 2633 2633
Size: 5723
SalI G'TCGA_C
Cuts at: 1321 2760 1321
Size: 1439 4284
SanDI GG'GwC_CC
Cuts at: 502 934 502
Size: 432 5291
SapI GCTCTTCn'nnn_
Cuts at: 1924 2347 3746 3956 1924
Size: 423 1399 210 3691
Fragments arranged by size:
3691 1399 423 210
SbfI CC_TGCA'GG
Cuts at: 1622 2770 1622
Size: 1148 4575
ScaI AGT'ACT
Cuts at: 5240 5240
Size: 5723
SexAI A'CCwGG_T
Cuts at: 452 602 644 683 452
Size: 150 42 39 5492
Fragments arranged by size:
5492 150 42 39
SfcI C'TryA_G
Cuts at: 33 1504 1618 1741 2113 2550 2766 3451 4444
Size: 1471 114 123 372 437 216 685 993
Cuts at: 4444 4635 33
Size: 191 1121
Fragments arranged by size:
1471 1121 993 685 437 372 216 191
123 114
SfoI GGC'GCC
Cuts at: 259 3155 3403 259
Size: 2896 248 2579
Fragments arranged by size:
2896 2579 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
SgrAI Cr'CCGG_yG
Cuts at: 1558 1558
Size: 5723
SmaI CCC'GGG
Cuts at: 500 836 1256 1946 2297 2745 500
Size: 336 420 690 351 448 3478
Fragments arranged by size:
3478 690 448 420 351 336
SmlI C'TyrA_G
Cuts at: 286 1825 2878 3793 4285 4547 286
Size: 1539 1053 915 492 262 1462
Fragments arranged by size:
1539 1462 1053 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5723
SphI G_CATG'C
Cuts at: 1723 2776 3276 1723
Size: 1053 500 4170
Fragments arranged by size:
4170 1053 500
SrfI GCCC'GGGC
Cuts at: 1256 1256
Size: 5723
StuI AGG'CCT
Cuts at: 485 485
Size: 5723
StyI C'CwwG_G
Cuts at: 698 920 1109 1436 1931 2516 2597 2697 2857
Size: 222 189 327 495 585 81 100 160
Cuts at: 2857 3211 698
Size: 354 3210
Fragments arranged by size:
3210 585 495 354 327 222 189 160
100 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2692 3306 3439 3898 4107 2692
Size: 614 133 459 209 4308
Fragments arranged by size:
4308 614 459 209 133
TatI w'GTAC_w
Cuts at: 401 5238 401
Size: 4837 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 252 5240 5272 220
Size: 32 4988 32 671
Fragments arranged by size:
4988 671 32 32
Tth111I GACn'n_nGTC
Cuts at: 445 2104 3520 445
Size: 1659 1416 2648
Fragments arranged by size:
2648 1659 1416
XbaI T'CTAG_A
Cuts at: 2754 2754
Size: 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 364 2439 3218 364
Size: 2075 779 2869
Fragments arranged by size:
2869 2075 779
XmaI C'CCGG_G
Cuts at: 498 834 1254 1944 2295 2743 498
Size: 336 420 690 351 448 3478
Fragments arranged by size:
3478 690 448 420 351 336
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI BaeI BcgI BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI
SfiI SnaBI SspI SwaI XhoI XmnI ZraI