Restriction map for
pF1KE1062
(Circular) (Six-base) MAPSORT of: pF1KE1062.seq Check: 9189 from: 1 to: 7475
pF1KE1062 7475 bp
With 182 enzymes: SgfI *
January 15, 2009 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1000 1560 2145 1000
Size: 560 585 6330
Fragments arranged by size:
6330 585 560
AccI GT'mk_AC
Cuts at: 3760 4513 3760
Size: 753 6722
Acc65I G'GTAC_C
Cuts at: 4491 4491
Size: 7475
AclI AA'CG_TT
Cuts at: 253 7315 253
Size: 7062 413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 908 1159 1469 2405 3272 4088 4367 5300
Size: 144 251 310 936 867 816 279 933
Cuts at: 5300 5732 6479 6747 764
Size: 432 747 268 1492
Fragments arranged by size:
1492 936 933 867 816 747 432 310
279 268 251 144
AfeI AGC'GCT
Cuts at: 2542 2542
Size: 7475
AflIII A'CryG_T
Cuts at: 1623 4827 5931 6908 1623
Size: 3204 1104 977 2190
Fragments arranged by size:
3204 2190 1104 977
AgeI A'CCGG_T
Cuts at: 5863 5863
Size: 7475
AhdI GACnn_n'nnGTC
Cuts at: 2337 2921 3756 2337
Size: 584 835 6056
Fragments arranged by size:
6056 835 584
AleI CACnn'nnGTG
Cuts at: 3436 3436
Size: 7475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 466 2153 466
Size: 1687 5788
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 243 275 243
Size: 32 7443
AlwNI CAG_nnn'CTG
Cuts at: 835 1858 2085 3289 4780 6347 835
Size: 1023 227 1204 1491 1567 1963
Fragments arranged by size:
1963 1567 1491 1204 1023 227
ApaLI G'TGCA_C
Cuts at: 6245 6245
Size: 7475
ApoI r'AATT_y
Cuts at: 1132 1871 2103 2899 3546 3723 4479 6828 7084
Size: 739 232 796 647 177 756 2349 256
Cuts at: 7084 1132
Size: 1523
Fragments arranged by size:
2349 1523 796 756 739 647 256 232
177
AseI AT'TA_AT
Cuts at: 20 1955 20
Size: 1935 5540
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7475
AvaI C'yCGr_G
Cuts at: 178 1493 3927 4495 178
Size: 1315 2434 568 3158
Fragments arranged by size:
3158 2434 1315 568
AvrII C'CTAG_G
Cuts at: 3318 3318
Size: 7475
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3166 3199 3166
Size: 33 7442
BamHI G'GATC_C
Cuts at: 2565 3322 3578 4500 2565
Size: 757 256 922 5540
Fragments arranged by size:
5540 922 757 256
BanI G'GyrC_C
Cuts at: 182 189 196 4491 4905 5153 5188 182
Size: 7 7 4295 414 248 35 2469
Fragments arranged by size:
4295 2469 414 248 35 7 7
BanII G_rGCy'C
Cuts at: 129 1377 3373 4489 5519 129
Size: 1248 1996 1116 1030 2085
Fragments arranged by size:
2085 1996 1248 1116 1030
BbeI G_GCGC'C
Cuts at: 186 193 200 4909 5157 186
Size: 7 7 4709 248 2504
Fragments arranged by size:
4709 2504 248 7 7
BbsI GAAGACnn'nnnn_
Cuts at: 550 4120 4285 4382 550
Size: 3570 165 97 3643
Fragments arranged by size:
3643 3570 165 97
BbvCI CC'TCA_GC
Cuts at: 139 139
Size: 7475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2732 2766 3491 3525 2732
Size: 34 725 34 6682
Fragments arranged by size:
6682 725 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 282 5373 6134 7475 282
Size: 5091 761 1341 282
Fragments arranged by size:
5091 1341 761 282
BclI T'GATC_A
Cuts at: 4053 4995 4053
Size: 942 6533
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 7475
BglII A'GATC_T
Cuts at: 3570 4990 7027 3570
Size: 1420 2037 4018
Fragments arranged by size:
4018 2037 1420
BlpI GC'TnA_GC
Cuts at: 4587 4587
Size: 7475
Bme1580I G_kGCm'C
Cuts at: 3843 4344 5100 5193 6249 3843
Size: 501 756 93 1056 5069
Fragments arranged by size:
5069 1056 756 501 93
BmgBI CAC'GTC
Cuts at: 1541 1541
Size: 7475
BmrI ACTGGGnnnn_n'
Cuts at: 2060 3141 4331 4687 4768 5103 7247 2060
Size: 1081 1190 356 81 335 2144 2288
Fragments arranged by size:
2288 2144 1190 1081 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1760 2459 4331 5842 1760
Size: 699 1872 1511 3393
Fragments arranged by size:
3393 1872 1511 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1391 2039 2126 2432 3311 3551 3605 3616
Size: 349 648 87 306 879 240 54 11
Cuts at: 3616 3870 3887 4178 1042
Size: 254 17 291 4339
Fragments arranged by size:
4339 879 648 349 306 291 254 240
87 54 17 11
Bpu10I CC'TnA_GC
Cuts at: 139 1728 139
Size: 1589 5886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 3653 4651 5530 6022 6320 1085
Size: 2568 998 879 492 298 2240
Fragments arranged by size:
2568 2240 998 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1521 2212 3780 4351 7179 1521
Size: 691 1568 571 2828 1817
Fragments arranged by size:
2828 1817 1568 691 571
BsaAI yAC'GTr
Cuts at: 1841 2406 2738 4828 1841
Size: 565 332 2090 4488
Fragments arranged by size:
4488 2090 565 332
BsaBI GATnn'nnATC
Cuts at: 6 4989 7026 6
Size: 4983 2037 455
Fragments arranged by size:
4983 2037 455
BsaHI Gr'CG_yC
Cuts at: 183 190 197 408 3425 4906 5154 5856 7352
Size: 7 7 211 3017 1481 248 702 1496
Cuts at: 7352 183
Size: 306
Fragments arranged by size:
3017 1496 1481 702 306 248 211 7
7
BsaWI w'CCGG_w
Cuts at: 4664 5185 5863 6137 6284 6695 4664
Size: 521 678 274 147 411 5444
Fragments arranged by size:
5444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 243 273 1404 1434 3633 3663 243
Size: 30 1131 30 2199 30 4055
Fragments arranged by size:
4055 2199 1131 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 979 1481 2066 2435 3371 4074 127
Size: 852 502 585 369 936 703 3528
Fragments arranged by size:
3528 936 852 703 585 502 369
BseYI C'CCAG_C
Cuts at: 601 1051 1788 2569 2817 3815 3824 4006 4244
Size: 450 737 781 248 998 9 182 238
Cuts at: 4244 4900 5658 6235 6731 601
Size: 656 758 577 496 1345
Fragments arranged by size:
1345 998 781 758 737 656 577 496
450 248 238 182 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4032 4372 4032
Size: 340 7135
BsiEI CG_ry'CG
Cuts at: 104 206 1688 5017 5063 6271 104
Size: 102 1482 3329 46 1208 1308
Fragments arranged by size:
3329 1482 1308 1208 102 46
BsiHKAI G_wGCw'C
Cuts at: 4489 5267 5457 6249 4489
Size: 778 190 792 5715
Fragments arranged by size:
5715 792 778 190
BsiWI C'GTAC_G
Cuts at: 2406 2406
Size: 7475
BsmI GAATG_Cn'
Cuts at: 245 453 725 2844 3120 245
Size: 208 272 2119 276 4600
Fragments arranged by size:
4600 2119 276 272 208
BsmBI CGTCTCn'nnnn_
Cuts at: 4040 4040
Size: 7475
Bsp1286I G_dGCh'C
Cuts at: 129 1377 3373 3843 4344 4489 5100 5193 5267
Size: 1248 1996 470 501 145 611 93 74
Cuts at: 5267 5457 5519 6249 129
Size: 190 62 730 1355
Fragments arranged by size:
1996 1355 1248 730 611 501 470 190
145 93 74 62
BspEI T'CCGG_A
Cuts at: 6695 6695
Size: 7475
BspHI T'CATG_A
Cuts at: 2718 3585 3638 6651 2718
Size: 867 53 3013 3542
Fragments arranged by size:
3542 3013 867 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 1000 1560 2145 4525 4739 5041 5422 544
Size: 456 560 585 2380 214 302 381 2597
Fragments arranged by size:
2597 2380 585 560 456 381 302 214
BsrBI CCG'CTC
Cuts at: 50 120 215 2415 3347 5767 5821 7305 7470
Size: 70 95 2200 932 2420 54 1484 165
Cuts at: 7470 50
Size: 55
Fragments arranged by size:
2420 2200 1484 932 165 95 70 55
54
BsrDI GCAATG_nn'
Cuts at: 450 741 1337 1869 4219 5387 450
Size: 291 596 532 2350 1168 2538
Fragments arranged by size:
2538 2350 1168 596 532 291
BsrFI r'CCGG_y
Cuts at: 282 3305 4310 5473 5654 5863 282
Size: 3023 1005 1163 181 209 1894
Fragments arranged by size:
3023 1894 1163 1005 209 181
BsrGI T'GTAC_A
Cuts at: 1691 1691
Size: 7475
BssSI C'ACGA_G
Cuts at: 2375 5746 6104 2375
Size: 3371 358 3746
Fragments arranged by size:
3746 3371 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4852 4852
Size: 7475
BstBI TT'CG_AA
Cuts at: 249 2046 3996 5836 249
Size: 1797 1950 1840 1888
Fragments arranged by size:
1950 1888 1840 1797
BstEII G'GTnAC_C
Cuts at: 2913 2913
Size: 7475
BstXI CCAn_nnnn'nTGG
Cuts at: 2526 3441 3575 4419 2526
Size: 915 134 844 5582
Fragments arranged by size:
5582 915 844 134
BstYI r'GATC_y
Cuts at: 1 427 831 1560 2565 2667 3322 3570 3578
Size: 426 404 729 1005 102 655 248 8
Cuts at: 3578 4500 4967 4990 5325 5571 6583 6669 6681
Size: 922 467 23 335 246 1012 86 12
Cuts at: 6681 7027 1
Size: 346 449
Fragments arranged by size:
1012 1005 922 729 655 467 449 426
404 346 335 248 246 102 86 23
12 8
Bsu36I CC'TnA_GG
Cuts at: 836 1769 3282 836
Size: 933 1513 5029
Fragments arranged by size:
5029 1513 933
BtgI C'CryG_G
Cuts at: 107 200 2955 3695 3937 107
Size: 93 2755 740 242 3645
Fragments arranged by size:
3645 2755 740 242 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5425 5606 5769 5425
Size: 181 163 7131
Fragments arranged by size:
7131 181 163
BtsI GCAGTG_nn'
Cuts at: 1477 1747 4133 4718 1477
Size: 270 2386 585 4234
Fragments arranged by size:
4234 2386 585 270
DraI TTT'AAA
Cuts at: 386 2879 4474 386
Size: 2493 1595 3387
Fragments arranged by size:
3387 2493 1595
DraIII CAC_nnn'GTG
Cuts at: 2583 2661 4275 2583
Size: 78 1614 5783
Fragments arranged by size:
5783 1614 78
DrdI GACnn_nn'nnGTC
Cuts at: 2241 5181 6039 2241
Size: 2940 858 3677
Fragments arranged by size:
3677 2940 858
EaeI y'GGCC_r
Cuts at: 203 497 4094 5060 5234 5625 5652 6997 203
Size: 294 3597 966 174 391 27 1345 681
Fragments arranged by size:
3597 1345 966 681 391 294 174 27
EagI C'GGCC_G
Cuts at: 203 5060 203
Size: 4857 2618
EarI CTCTTCn'nnn_
Cuts at: 315 2405 4035 4159 5498 5708 315
Size: 2090 1630 124 1339 210 2082
Fragments arranged by size:
2090 2082 1630 1339 210 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 1560 5683 5993 6139 7285 130
Size: 1430 4123 310 146 1146 320
Fragments arranged by size:
4123 1430 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 4487 4487
Size: 7475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 908 1042 1159 1391 1469 2039 2126 2405
Size: 144 134 117 232 78 570 87 279
Cuts at: 2405 2432 3272 3311 3551 3605 3616 3870 3887
Size: 27 840 39 240 54 11 254 17
Cuts at: 3887 4088 4178 4367 5300 5732 6479 6747 764
Size: 201 90 189 933 432 747 268 1492
Fragments arranged by size:
1492 933 840 747 570 432 279 268
254 240 232 201 189 144 134 117
90 87 78 54 39 27 17 11
EcoNI CCTnn'n_nnAGG
Cuts at: 476 476
Size: 7475
EcoO109I rG'GnC_Cy
Cuts at: 3241 4062 4198 4446 4614 3241
Size: 821 136 248 168 6102
Fragments arranged by size:
6102 821 248 168 136
EcoRI G'AATT_C
Cuts at: 1132 2899 3546 4479 1132
Size: 1767 647 933 4128
Fragments arranged by size:
4128 1767 933 647
EcoRV GAT'ATC
Cuts at: 845 3675 845
Size: 2830 4645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1126 1961 5494 1126
Size: 835 3533 3107
Fragments arranged by size:
3533 3107 835
FspI TGC'GCA
Cuts at: 5256 5256
Size: 7475
HaeII r_GCGC'y
Cuts at: 186 193 200 2544 4909 5157 6179 7158 186
Size: 7 7 2344 2365 248 1022 979 503
Fragments arranged by size:
2365 2344 1022 979 503 248 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 829 861 2244 2276 2323 2355 2557
Size: 32 517 32 1383 32 47 32 202
Cuts at: 2557 2589 2637 2669 2907 2939 3307 3339 3633
Size: 32 48 32 238 32 368 32 294
Cuts at: 3633 3665 3742 3774 5317 5347 5349 5379 280
Size: 32 77 32 1543 30 2 30 2376
Fragments arranged by size:
2376 1543 1383 517 368 294 238 202
77 48 47 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3185 3687 4514 3185
Size: 502 827 6146
Fragments arranged by size:
6146 827 502
HpaI GTT'AAC
Cuts at: 3185 3185
Size: 7475
KasI G'GCGC_C
Cuts at: 182 189 196 4905 5153 182
Size: 7 7 4709 248 2504
Fragments arranged by size:
4709 2504 248 7 7
KpnI G_GTAC'C
Cuts at: 4495 4495
Size: 7475
MfeI C'AATT_G
Cuts at: 1091 1091
Size: 7475
MluI A'CGCG_T
Cuts at: 6908 6908
Size: 7475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 964 1240 1335 3004 3350 4352 6146 6330 964
Size: 276 95 1669 346 1002 1794 184 2109
Fragments arranged by size:
2109 1794 1669 1002 346 276 184 95
MscI TGG'CCA
Cuts at: 499 4096 5236 499
Size: 3597 1140 2738
Fragments arranged by size:
3597 2738 1140
MslI CAynn'nnrTG
Cuts at: 2431 2524 2677 3040 3436 3439 3955 4366 5464
Size: 93 153 363 396 3 516 411 1098
Cuts at: 5464 5591 2431
Size: 127 4315
Fragments arranged by size:
4315 1098 516 411 396 363 153 127
93 3
MspA1I CmG'CkG
Cuts at: 202 2241 2250 4586 4900 5260 6273 6518 202
Size: 2039 9 2336 314 360 1013 245 1159
Fragments arranged by size:
2336 2039 1159 1013 360 314 245 9
NaeI GCC'GGC
Cuts at: 3307 4312 5656 3307
Size: 1005 1344 5126
Fragments arranged by size:
5126 1344 1005
NarI GG'CG_CC
Cuts at: 183 190 197 4906 5154 183
Size: 7 7 4709 248 2504
Fragments arranged by size:
4709 2504 248 7 7
NcoI C'CATG_G
Cuts at: 107 2955 3695 3937 107
Size: 2848 740 242 3645
Fragments arranged by size:
3645 2848 740 242
NdeI CA'TA_TG
Cuts at: 1809 1809
Size: 7475
NgoMIV G'CCGG_C
Cuts at: 3305 4310 5654 3305
Size: 1005 1344 5126
Fragments arranged by size:
5126 1344 1005
NotI GC'GGCC_GC
Cuts at: 203 203
Size: 7475
NspI r_CATG'y
Cuts at: 3759 4046 4528 5028 5935 3759
Size: 287 482 500 907 5299
Fragments arranged by size:
5299 907 500 482 287
PciI A'CATG_T
Cuts at: 5931 5931
Size: 7475
PflMI CCAn_nnn'nTGG
Cuts at: 950 1858 950
Size: 908 6567
PfoI T'CCnGG_A
Cuts at: 419 2016 4194 419
Size: 1597 2178 3700
Fragments arranged by size:
3700 2178 1597
PmeI GTTT'AAAC
Cuts at: 4474 4474
Size: 7475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 243 275 6614 6646 243
Size: 32 6339 32 1072
Fragments arranged by size:
6339 1072 32 32
PpuMI rG'GwC_Cy
Cuts at: 3241 4062 4198 4446 3241
Size: 821 136 248 6270
Fragments arranged by size:
6270 821 248 136
PshAI GACnn'nnGTC
Cuts at: 3246 3246
Size: 7475
PsiI TTA'TAA
Cuts at: 1883 1883
Size: 7475
PstI C_TGCA'G
Cuts at: 4522 5207 4522
Size: 685 6790
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7475
PvuII CAG'CTG
Cuts at: 2241 2250 4900 5260 2241
Size: 9 2650 360 4456
Fragments arranged by size:
4456 2650 360 9
RsrII CG'GwC_CG
Cuts at: 5670 5670
Size: 7475
SacI G_AGCT'C
Cuts at: 4489 4489
Size: 7475
SacII CC_GC'GG
Cuts at: 203 203
Size: 7475
SalI G'TCGA_C
Cuts at: 4512 4512
Size: 7475
SanDI GG'GwC_CC
Cuts at: 3241 3241
Size: 7475
SapI GCTCTTCn'nnn_
Cuts at: 315 4159 5498 5708 315
Size: 3844 1339 210 2082
Fragments arranged by size:
3844 2082 1339 210
SbfI CC_TGCA'GG
Cuts at: 4522 4522
Size: 7475
ScaI AGT'ACT
Cuts at: 6992 6992
Size: 7475
SfcI C'TryA_G
Cuts at: 33 4518 5203 6196 6387 33
Size: 4485 685 993 191 1121
Fragments arranged by size:
4485 1121 993 685 191
SfoI GGC'GCC
Cuts at: 184 191 198 4907 5155 184
Size: 7 7 4709 248 2504
Fragments arranged by size:
4709 2504 248 7 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7475
SmaI CCC'GGG
Cuts at: 3929 4497 3929
Size: 568 6907
SmlI C'TyrA_G
Cuts at: 1100 3668 4630 5545 6037 6299 1100
Size: 2568 962 915 492 262 2276
Fragments arranged by size:
2568 2276 962 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7475
SphI G_CATG'C
Cuts at: 4528 5028 4528
Size: 500 6975
StuI AGG'CCT
Cuts at: 2194 2652 2194
Size: 458 7017
StyI C'CwwG_G
Cuts at: 107 1601 2090 2222 2955 2993 3318 3695 3937
Size: 1494 489 132 733 38 325 377 242
Cuts at: 3937 4609 4963 107
Size: 672 354 2619
Fragments arranged by size:
2619 1494 733 672 489 377 354 325
242 132 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1658 2602 3006 4172 5058 5191 5650 5859 1658
Size: 944 404 1166 886 133 459 209 3274
Fragments arranged by size:
3274 1166 944 886 459 404 209 133
TatI w'GTAC_w
Cuts at: 1691 1889 2831 2935 3103 3536 6990 1691
Size: 198 942 104 168 433 3454 2176
Fragments arranged by size:
3454 2176 942 433 198 168 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4197 4229 6992 7024 4197
Size: 32 2763 32 4648
Fragments arranged by size:
4648 2763 32 32
Tth111I GACn'n_nGTC
Cuts at: 5272 5272
Size: 7475
XbaI T'CTAG_A
Cuts at: 4506 4506
Size: 7475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 359 656 947 1223 2559 3381 4368 4970 359
Size: 297 291 276 1336 822 987 602 2864
Fragments arranged by size:
2864 1336 987 822 602 297 291 276
XmaI C'CCGG_G
Cuts at: 3927 4495 3927
Size: 568 6907
XmnI GAAnn'nnTTC
Cuts at: 2975 3992 4168 2975
Size: 1017 176 6282
Fragments arranged by size:
6282 1017 176
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII ApaI AscI BfrBI BmtI BssHII BstZ17I
ClaI CspCI FseI FspAI HindIII NheI NruI NsiI
PacI PasI PmlI PspOMI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI