Restriction map for
pF1KE1063
(Circular) (Six-base) MAPSORT of: pF1KE1063.seq Check: 4076 from: 1 to: 4856
pF1KE1063 4856 bp
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 334 334
Size: 4856
AccI GT'mk_AC
Cuts at: 1894 1894
Size: 4856
Acc65I G'GTAC_C
Cuts at: 1872 1872
Size: 4856
AclI AA'CG_TT
Cuts at: 4696 4696
Size: 4856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 472 873 1272 1608 1692 2681 3113 3860
Size: 208 401 399 336 84 989 432 747
Cuts at: 3860 4128 264
Size: 268 992
Fragments arranged by size:
992 989 747 432 401 399 336 268
208 84
AflIII A'CryG_T
Cuts at: 143 2208 3312 4289 143
Size: 2065 1104 977 710
Fragments arranged by size:
2065 1104 977 710
AgeI A'CCGG_T
Cuts at: 3244 3244
Size: 4856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4856
AlwNI CAG_nnn'CTG
Cuts at: 180 1778 2161 3728 180
Size: 1598 383 1567 1308
Fragments arranged by size:
1598 1567 1308 383
ApaLI G'TGCA_C
Cuts at: 3626 3626
Size: 4856
ApoI r'AATT_y
Cuts at: 412 1157 1322 1574 1658 1860 4209 4465 412
Size: 745 165 252 84 202 2349 256 803
Fragments arranged by size:
2349 803 745 256 252 202 165 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
AvaI C'yCGr_G
Cuts at: 1876 1876
Size: 4856
BamHI G'GATC_C
Cuts at: 1881 1881
Size: 4856
BanI G'GyrC_C
Cuts at: 1872 2286 2534 2569 1872
Size: 414 248 35 4159
Fragments arranged by size:
4159 414 248 35
BanII G_rGCy'C
Cuts at: 1870 2900 1870
Size: 1030 3826
BbeI G_GCGC'C
Cuts at: 2290 2538 2290
Size: 248 4608
BbsI GAAGACnn'nnnn_
Cuts at: 1372 1372
Size: 4856
BciVI GTATCCnnnnn_n'
Cuts at: 2754 3515 4856 2754
Size: 761 1341 2754
Fragments arranged by size:
2754 1341 761
BclI T'GATC_A
Cuts at: 2376 2376
Size: 4856
BfrBI ATG'CAT
Cuts at: 1494 1494
Size: 4856
BglII A'GATC_T
Cuts at: 2371 4408 2371
Size: 2037 2819
BlpI GC'TnA_GC
Cuts at: 1968 1968
Size: 4856
Bme1580I G_kGCm'C
Cuts at: 2481 2574 3630 2481
Size: 93 1056 3707
Fragments arranged by size:
3707 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 4856
BmrI ACTGGGnnnn_n'
Cuts at: 773 1211 2068 2149 2484 4628 773
Size: 438 857 81 335 2144 1001
Fragments arranged by size:
2144 1001 857 438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 4856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 2032 2911 3403 3701 241
Size: 1791 879 492 298 1396
Fragments arranged by size:
1791 1396 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1584 4560 1584
Size: 2976 1880
BsaAI yAC'GTr
Cuts at: 2209 2209
Size: 4856
BsaBI GATnn'nnATC
Cuts at: 6 2370 4407 6
Size: 2364 2037 455
Fragments arranged by size:
2364 2037 455
BsaHI Gr'CG_yC
Cuts at: 2287 2535 3237 4733 2287
Size: 248 702 1496 2410
Fragments arranged by size:
2410 1496 702 248
BsaWI w'CCGG_w
Cuts at: 847 2045 2566 3244 3518 3665 4076 847
Size: 1198 521 678 274 147 411 1627
Fragments arranged by size:
1627 1198 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 518 548 518
Size: 30 4826
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 4856
BseYI C'CCAG_C
Cuts at: 2281 3039 3616 4112 2281
Size: 758 577 496 3025
Fragments arranged by size:
3025 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1203 362
Size: 841 4015
BsiEI CG_ry'CG
Cuts at: 104 2398 2444 3652 104
Size: 2294 46 1208 1308
Fragments arranged by size:
2294 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1870 2648 2838 3630 1870
Size: 778 190 792 3096
Fragments arranged by size:
3096 792 778 190
BsmI GAATG_Cn'
Cuts at: 430 871 955 1438 1496 1606 1774 1789 430
Size: 441 84 483 58 110 168 15 3497
Fragments arranged by size:
3497 483 441 168 110 84 58 15
Bsp1286I G_dGCh'C
Cuts at: 1870 2481 2574 2648 2838 2900 3630 1870
Size: 611 93 74 190 62 730 3096
Fragments arranged by size:
3096 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4076 4076
Size: 4856
BspHI T'CATG_A
Cuts at: 4032 4032
Size: 4856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 687 1906 2120 2422 2803 334
Size: 353 1219 214 302 381 2387
Fragments arranged by size:
2387 1219 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 3148 3202 4686 4851 50
Size: 3098 54 1484 165 55
Fragments arranged by size:
3098 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2768 2768
Size: 4856
BsrFI r'CCGG_y
Cuts at: 2854 3035 3244 2854
Size: 181 209 4466
Fragments arranged by size:
4466 209 181
BsrGI T'GTAC_A
Cuts at: 828 1479 828
Size: 651 4205
BssSI C'ACGA_G
Cuts at: 3127 3485 3127
Size: 358 4498
BstAPI GCAn_nnn'nTGC
Cuts at: 2233 2233
Size: 4856
BstBI TT'CG_AA
Cuts at: 3217 3217
Size: 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 365 365
Size: 4856
BstYI r'GATC_y
Cuts at: 1 1881 2348 2371 2706 2952 3964 4050 4062
Size: 1880 467 23 335 246 1012 86 12
Cuts at: 4062 4408 1
Size: 346 449
Fragments arranged by size:
1880 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 245 107
Size: 138 4718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2806 2987 3150 2806
Size: 181 163 4512
Fragments arranged by size:
4512 181 163
BtsI GCAGTG_nn'
Cuts at: 960 1191 2099 960
Size: 231 908 3717
Fragments arranged by size:
3717 908 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1509 1544 1509
Size: 35 4821
DraI TTT'AAA
Cuts at: 578 1855 578
Size: 1277 3579
DraIII CAC_nnn'GTG
Cuts at: 240 240
Size: 4856
DrdI GACnn_nn'nnGTC
Cuts at: 2562 3420 2562
Size: 858 3998
EaeI y'GGCC_r
Cuts at: 110 2441 2615 3006 3033 4378 110
Size: 2331 174 391 27 1345 588
Fragments arranged by size:
2331 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2441 2441
Size: 4856
EarI CTCTTCn'nnn_
Cuts at: 2879 3089 2879
Size: 210 4646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3064 3374 3520 4666 3064
Size: 310 146 1146 3254
Fragments arranged by size:
3254 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1868 1868
Size: 4856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 264 472 873 1272 1608 1692 2681 3113
Size: 80 208 401 399 336 84 989 432
Cuts at: 3113 3860 4128 184
Size: 747 268 912
Fragments arranged by size:
989 912 747 432 401 399 336 268
208 84 80
EcoNI CCTnn'n_nnAGG
Cuts at: 1626 1626
Size: 4856
EcoO109I rG'GnC_Cy
Cuts at: 323 1995 323
Size: 1672 3184
EcoRI G'AATT_C
Cuts at: 1157 1322 1574 1658 1860 1157
Size: 165 252 84 202 4153
Fragments arranged by size:
4153 252 202 165 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4856
FspI TGC'GCA
Cuts at: 2637 2637
Size: 4856
HaeII r_GCGC'y
Cuts at: 2290 2538 3560 4539 2290
Size: 248 1022 979 2607
Fragments arranged by size:
2607 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 984 1016 1053 1085 1551 1583 2698 2728 2730
Size: 32 37 32 466 32 1115 30 2
Cuts at: 2730 2760 984
Size: 30 3080
Fragments arranged by size:
3080 1115 466 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 375 902 986 1469 1637 1805 1895 375
Size: 527 84 483 168 168 90 3336
Fragments arranged by size:
3336 527 483 168 168 90 84
HindIII A'AGCT_T
Cuts at: 1284 1284
Size: 4856
KasI G'GCGC_C
Cuts at: 2286 2534 2286
Size: 248 4608
KpnI G_GTAC'C
Cuts at: 1876 1876
Size: 4856
MluI A'CGCG_T
Cuts at: 4289 4289
Size: 4856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3527 3711 3527
Size: 184 4672
MscI TGG'CCA
Cuts at: 112 2617 112
Size: 2505 2351
MslI CAynn'nnrTG
Cuts at: 112 1032 1166 2845 2972 112
Size: 920 134 1679 127 1996
Fragments arranged by size:
1996 1679 920 134 127
MspA1I CmG'CkG
Cuts at: 1967 2281 2641 3654 3899 1967
Size: 314 360 1013 245 2924
Fragments arranged by size:
2924 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3037 3037
Size: 4856
NarI GG'CG_CC
Cuts at: 2287 2535 2287
Size: 248 4608
NcoI C'CATG_G
Cuts at: 107 107
Size: 4856
NgoMIV G'CCGG_C
Cuts at: 3035 3035
Size: 4856
NsiI A_TGCA'T
Cuts at: 1496 1496
Size: 4856
NspI r_CATG'y
Cuts at: 1909 2409 3316 1909
Size: 500 907 3449
Fragments arranged by size:
3449 907 500
PciI A'CATG_T
Cuts at: 3312 3312
Size: 4856
PfoI T'CCnGG_A
Cuts at: 304 304
Size: 4856
PmeI GTTT'AAAC
Cuts at: 1855 1855
Size: 4856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 3995
Size: 32 4824
PpuMI rG'GwC_Cy
Cuts at: 323 323
Size: 4856
PshAI GACnn'nnGTC
Cuts at: 1043 1043
Size: 4856
PsiI TTA'TAA
Cuts at: 659 1115 659
Size: 456 4400
PstI C_TGCA'G
Cuts at: 684 1903 2588 684
Size: 1219 685 2952
Fragments arranged by size:
2952 1219 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4856
PvuII CAG'CTG
Cuts at: 2281 2641 2281
Size: 360 4496
RsrII CG'GwC_CG
Cuts at: 3051 3051
Size: 4856
SacI G_AGCT'C
Cuts at: 1870 1870
Size: 4856
SalI G'TCGA_C
Cuts at: 1893 1893
Size: 4856
SanDI GG'GwC_CC
Cuts at: 323 323
Size: 4856
SapI GCTCTTCn'nnn_
Cuts at: 2879 3089 2879
Size: 210 4646
SbfI CC_TGCA'GG
Cuts at: 1903 1903
Size: 4856
ScaI AGT'ACT
Cuts at: 4373 4373
Size: 4856
SexAI A'CCwGG_T
Cuts at: 236 291 236
Size: 55 4801
SfcI C'TryA_G
Cuts at: 33 204 228 680 1899 2584 3577 3768 33
Size: 171 24 452 1219 685 993 191 1121
Fragments arranged by size:
1219 1121 993 685 452 191 171 24
SfoI GGC'GCC
Cuts at: 2288 2536 2288
Size: 248 4608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
SmaI CCC'GGG
Cuts at: 1878 1878
Size: 4856
SmlI C'TyrA_G
Cuts at: 220 2011 2926 3418 3680 220
Size: 1791 915 492 262 1396
Fragments arranged by size:
1791 1396 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4856
SphI G_CATG'C
Cuts at: 1909 2409 1909
Size: 500 4356
SspI AAT'ATT
Cuts at: 544 544
Size: 4856
StuI AGG'CCT
Cuts at: 1705 1705
Size: 4856
StyI C'CwwG_G
Cuts at: 107 1990 2344 107
Size: 1883 354 2619
Fragments arranged by size:
2619 1883 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2439 2572 3031 3240 2439
Size: 133 459 209 4055
Fragments arranged by size:
4055 459 209 133
TatI w'GTAC_w
Cuts at: 784 828 1129 1479 4371 784
Size: 44 301 350 2892 1269
Fragments arranged by size:
2892 1269 350 301 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 226 258 4373 4405 226
Size: 32 4115 32 677
Fragments arranged by size:
4115 677 32 32
Tth111I GACn'n_nGTC
Cuts at: 2653 2653
Size: 4856
XbaI T'CTAG_A
Cuts at: 1887 1887
Size: 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2351
Size: 4856
XmaI C'CCGG_G
Cuts at: 1876 1876
Size: 4856
XmnI GAAnn'nnTTC
Cuts at: 1285 1369 1705 1789 1285
Size: 84 336 84 4352
Fragments arranged by size:
4352 336 84 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI