Restriction map for
pF1KE1065
(Circular) (Six-base) MAPSORT of: pF1KE1065.seq Check: 2657 from: 1 to: 6077
pF1KE1065 6077 bp
With 182 enzymes: SgfI *
February 26, 2009 15:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1075 1075
Size: 6077
AccI GT'mk_AC
Cuts at: 203 3115 203
Size: 2912 3165
Acc65I G'GTAC_C
Cuts at: 3093 3093
Size: 6077
AclI AA'CG_TT
Cuts at: 5917 5917
Size: 6077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3902 4334 5081 5349 3902
Size: 432 747 268 4630
Fragments arranged by size:
4630 747 432 268
AflIII A'CryG_T
Cuts at: 781 2144 3429 4533 5510 781
Size: 1363 1285 1104 977 1348
Fragments arranged by size:
1363 1348 1285 1104 977
AgeI A'CCGG_T
Cuts at: 4465 4465
Size: 6077
AleI CACnn'nnGTG
Cuts at: 755 892 2024 755
Size: 137 1132 4808
Fragments arranged by size:
4808 1132 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1507 1507
Size: 6077
AlwNI CAG_nnn'CTG
Cuts at: 889 1146 1650 1839 1909 3382 4949 889
Size: 257 504 189 70 1473 1567 2017
Fragments arranged by size:
2017 1567 1473 504 257 189 70
ApaI G_GGCC'C
Cuts at: 1359 1529 1359
Size: 170 5907
ApaLI G'TGCA_C
Cuts at: 2329 4847 2329
Size: 2518 3559
ApoI r'AATT_y
Cuts at: 2912 3081 5430 5686 2912
Size: 169 2349 256 3303
Fragments arranged by size:
3303 2349 256 169
AseI AT'TA_AT
Cuts at: 20 20
Size: 6077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
AvaI C'yCGr_G
Cuts at: 390 1128 1275 1352 1522 2242 2404 3097 390
Size: 738 147 77 170 720 162 693 3370
Fragments arranged by size:
3370 738 720 693 170 162 147 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 390 423 390
Size: 33 6044
BamHI G'GATC_C
Cuts at: 2612 2774 3102 2612
Size: 162 328 5587
Fragments arranged by size:
5587 328 162
BanI G'GyrC_C
Cuts at: 1507 1709 2014 2134 2296 2392 2882 3093 3507
Size: 202 305 120 162 96 490 211 414
Cuts at: 3507 3755 3790 1507
Size: 248 35 3794
Fragments arranged by size:
3794 490 414 305 248 211 202 162
120 96 35
BanII G_rGCy'C
Cuts at: 191 1359 1529 2036 2243 3091 4121 191
Size: 1168 170 507 207 848 1030 2147
Fragments arranged by size:
2147 1168 1030 848 507 207 170
BbeI G_GCGC'C
Cuts at: 1713 2886 3511 3759 1713
Size: 1173 625 248 4031
Fragments arranged by size:
4031 1173 625 248
BbsI GAAGACnn'nnnn_
Cuts at: 332 946 332
Size: 614 5463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 813 847 887 921 1854 1888 2004 2038 2439
Size: 34 40 34 933 34 116 34 401
Cuts at: 2439 2473 813
Size: 34 4417
Fragments arranged by size:
4417 933 401 116 40 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3975 4736 6077 3975
Size: 761 1341 3975
Fragments arranged by size:
3975 1341 761
BclI T'GATC_A
Cuts at: 2933 3597 2933
Size: 664 5413
BglI GCCn_nnn'nGGC
Cuts at: 542 1037 2222 2390 542
Size: 495 1185 168 4229
Fragments arranged by size:
4229 1185 495 168
BglII A'GATC_T
Cuts at: 497 2633 3592 5629 497
Size: 2136 959 2037 945
Fragments arranged by size:
2136 2037 959 945
BlpI GC'TnA_GC
Cuts at: 3189 3189
Size: 6077
Bme1580I G_kGCm'C
Cuts at: 160 229 630 1359 1529 2107 2139 2227 2333
Size: 69 401 729 170 578 32 88 106
Cuts at: 2333 2825 3702 3795 4851 160
Size: 492 877 93 1056 1386
Fragments arranged by size:
1386 1056 877 729 578 492 401 170
106 93 88 69 32
BmgBI CAC'GTC
Cuts at: 207 839 2699 2952 207
Size: 632 1860 253 3332
Fragments arranged by size:
3332 1860 632 253
BmrI ACTGGGnnnn_n'
Cuts at: 3289 3370 3705 5849 3289
Size: 81 335 2144 3517
Fragments arranged by size:
3517 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 4444 2708
Size: 1736 4341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1966 2156 1966
Size: 190 5887
Bpu10I CC'TnA_GC
Cuts at: 1456 2237 1456
Size: 781 5296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 3253 4132 4624 4922 1669
Size: 1584 879 492 298 2824
Fragments arranged by size:
2824 1584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1627 1905 2710 5781 1627
Size: 278 805 3071 1923
Fragments arranged by size:
3071 1923 805 278
BsaAI yAC'GTr
Cuts at: 782 827 2228 3430 782
Size: 45 1401 1202 3429
Fragments arranged by size:
3429 1401 1202 45
BsaBI GATnn'nnATC
Cuts at: 6 2617 3591 5628 6
Size: 2611 974 2037 455
Fragments arranged by size:
2611 2037 974 455
BsaHI Gr'CG_yC
Cuts at: 646 1710 2883 3508 3756 4458 5954 646
Size: 1064 1173 625 248 702 1496 769
Fragments arranged by size:
1496 1173 1064 769 702 625 248
BsaWI w'CCGG_w
Cuts at: 866 3266 3787 4465 4739 4886 5297 866
Size: 2400 521 678 274 147 411 1646
Fragments arranged by size:
2400 1646 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1072 1102 1072
Size: 30 6047
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1686 1686
Size: 6077
BseYI C'CCAG_C
Cuts at: 1358 1528 2107 2562 3502 4260 4837 5333 1358
Size: 170 579 455 940 758 577 496 2102
Fragments arranged by size:
2102 940 758 579 577 496 455 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 1224 696
Size: 528 5549
BsiEI CG_ry'CG
Cuts at: 104 122 822 867 1878 2189 3619 3665 4873
Size: 18 700 45 1011 311 1430 46 1208
Cuts at: 4873 104
Size: 1308
Fragments arranged by size:
1430 1308 1208 1011 700 311 46 45
18
BsiHKAI G_wGCw'C
Cuts at: 220 1090 2036 2333 3091 3869 4059 4851 220
Size: 870 946 297 758 778 190 792 1446
Fragments arranged by size:
1446 946 870 792 778 758 297 190
BsmI GAATG_Cn'
Cuts at: 2581 2581
Size: 6077
BsmBI CGTCTCn'nnnn_
Cuts at: 637 829 637
Size: 192 5885
Bsp1286I G_dGCh'C
Cuts at: 160 191 220 229 630 1090 1359 1529 2036
Size: 31 29 9 401 460 269 170 507
Cuts at: 2036 2107 2139 2227 2243 2333 2825 3091 3702
Size: 71 32 88 16 90 492 266 611
Cuts at: 3702 3795 3869 4059 4121 4851 160
Size: 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 611 507 492 460 401 269
266 190 170 93 90 88 74 71
62 32 31 29 16 9
BspEI T'CCGG_A
Cuts at: 5297 5297
Size: 6077
BspHI T'CATG_A
Cuts at: 2675 5253 2675
Size: 2578 3499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 580 1075 2728 3127 3341 3643 4024 418
Size: 162 495 1653 399 214 302 381 2471
Fragments arranged by size:
2471 1653 495 399 381 302 214 162
BsrBI CCG'CTC
Cuts at: 50 584 1098 2759 4369 4423 5907 6072 50
Size: 534 514 1661 1610 54 1484 165 55
Fragments arranged by size:
1661 1610 1484 534 514 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3989 3989
Size: 6077
BsrFI r'CCGG_y
Cuts at: 1877 2251 2524 4075 4256 4465 1877
Size: 374 273 1551 181 209 3489
Fragments arranged by size:
3489 1551 374 273 209 181
BssSI C'ACGA_G
Cuts at: 373 1622 4348 4706 373
Size: 1249 2726 358 1744
Fragments arranged by size:
2726 1744 1249 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1650 2102 2255 2381 3454 1650
Size: 452 153 126 1073 4273
Fragments arranged by size:
4273 1073 452 153 126
BstBI TT'CG_AA
Cuts at: 4438 4438
Size: 6077
BstXI CCAn_nnnn'nTGG
Cuts at: 1944 2026 1944
Size: 82 5995
BstYI r'GATC_y
Cuts at: 1 497 1580 2612 2633 2774 3102 3569 3592
Size: 496 1083 1032 21 141 328 467 23
Cuts at: 3592 3927 4173 5185 5271 5283 5629 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1083 1032 1012 496 467 449 346 335
328 246 141 86 23 21 12
BtgI C'CryG_G
Cuts at: 107 512 618 1007 1155 1713 2219 3013 107
Size: 405 106 389 148 558 506 794 3171
Fragments arranged by size:
3171 794 558 506 405 389 148 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 912 2574 2678 2727 2891 4027 4208 4371 912
Size: 1662 104 49 164 1136 181 163 2618
Fragments arranged by size:
2618 1662 1136 181 164 163 104 49
BtsI GCAGTG_nn'
Cuts at: 325 922 1182 1659 2331 2421 2481 2496 3320
Size: 597 260 477 672 90 60 15 824
Cuts at: 3320 325
Size: 3082
Fragments arranged by size:
3082 824 672 597 477 260 90 60
15
ClaI AT'CG_AT
Cuts at: 245 245
Size: 6077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 465 500 465
Size: 35 6042
DraI TTT'AAA
Cuts at: 3076 3076
Size: 6077
DraIII CAC_nnn'GTG
Cuts at: 2102 2102
Size: 6077
DrdI GACnn_nn'nnGTC
Cuts at: 3783 4641 3783
Size: 858 5219
EaeI y'GGCC_r
Cuts at: 119 173 1716 1875 2037 2186 2343 2412 3662
Size: 54 1543 159 162 149 157 69 1250
Cuts at: 3662 3836 4227 4254 5599 119
Size: 174 391 27 1345 597
Fragments arranged by size:
1543 1345 1250 597 391 174 162 159
157 149 69 54 27
EagI C'GGCC_G
Cuts at: 119 1875 2186 3662 119
Size: 1756 311 1476 2534
Fragments arranged by size:
2534 1756 1476 311
EarI CTCTTCn'nnn_
Cuts at: 1429 1699 1778 2677 2950 4100 4310 1429
Size: 270 79 899 273 1150 210 3196
Fragments arranged by size:
3196 1150 899 273 270 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4285 4595 4741 5887 4285
Size: 310 146 1146 4475
Fragments arranged by size:
4475 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2034 3089 2034
Size: 1055 5022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1966 2156 3902 4334 5081 5349 1966
Size: 190 1746 432 747 268 2694
Fragments arranged by size:
2694 1746 747 432 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 2733 2733
Size: 6077
EcoO109I rG'GnC_Cy
Cuts at: 775 2940 3216 775
Size: 2165 276 3636
Fragments arranged by size:
3636 2165 276
EcoRI G'AATT_C
Cuts at: 3081 3081
Size: 6077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1590 2961 4096 1590
Size: 1371 1135 3571
Fragments arranged by size:
3571 1371 1135
FspI TGC'GCA
Cuts at: 843 1864 3858 843
Size: 1021 1994 3062
Fragments arranged by size:
3062 1994 1021
HaeII r_GCGC'y
Cuts at: 1492 1713 1945 2886 3511 3759 4781 5760 1492
Size: 221 232 941 625 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 941 625 248 232 221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 1072 1104 3919 3949 3951 3981 202
Size: 32 838 32 2815 30 2 30 2298
Fragments arranged by size:
2815 2298 838 32 32 30 30 2
HincII GTy'rAC
Cuts at: 204 972 1242 3116 204
Size: 768 270 1874 3165
Fragments arranged by size:
3165 1874 768 270
KasI G'GCGC_C
Cuts at: 1709 2882 3507 3755 1709
Size: 1173 625 248 4031
Fragments arranged by size:
4031 1173 625 248
KpnI G_GTAC'C
Cuts at: 3097 3097
Size: 6077
MluI A'CGCG_T
Cuts at: 5510 5510
Size: 6077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 894 2129 4748 4932 894
Size: 1235 2619 184 2039
Fragments arranged by size:
2619 2039 1235 184
MscI TGG'CCA
Cuts at: 2414 3838 2414
Size: 1424 4653
MslI CAynn'nnrTG
Cuts at: 245 623 755 892 1664 2024 4066 4193 245
Size: 378 132 137 772 360 2042 127 2129
Fragments arranged by size:
2129 2042 772 378 360 137 132 127
MspA1I CmG'CkG
Cuts at: 185 755 905 1157 1362 1532 1650 2372 2381
Size: 570 150 252 205 170 118 722 9
Cuts at: 2381 2804 3188 3502 3862 4875 5120 185
Size: 423 384 314 360 1013 245 1142
Fragments arranged by size:
1142 1013 722 570 423 384 360 314
252 245 205 170 150 118 9
NaeI GCC'GGC
Cuts at: 1879 4258 1879
Size: 2379 3698
NarI GG'CG_CC
Cuts at: 1710 2883 3508 3756 1710
Size: 1173 625 248 4031
Fragments arranged by size:
4031 1173 625 248
NcoI C'CATG_G
Cuts at: 107 512 618 107
Size: 405 106 5566
Fragments arranged by size:
5566 405 106
NgoMIV G'CCGG_C
Cuts at: 1877 4256 1877
Size: 2379 3698
NspI r_CATG'y
Cuts at: 875 947 1802 1964 3130 3630 4537 875
Size: 72 855 162 1166 500 907 2415
Fragments arranged by size:
2415 1166 907 855 500 162 72
PasI CC'CwG_GG
Cuts at: 153 193 153
Size: 40 6037
PciI A'CATG_T
Cuts at: 4533 4533
Size: 6077
PflMI CCAn_nnn'nTGG
Cuts at: 2076 2076
Size: 6077
PfoI T'CCnGG_A
Cuts at: 2309 2309
Size: 6077
PmeI GTTT'AAAC
Cuts at: 3076 3076
Size: 6077
PmlI CAC'GTG
Cuts at: 782 827 782
Size: 45 6032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5216 5248 5216
Size: 32 6045
PshAI GACnn'nnGTC
Cuts at: 467 467
Size: 6077
PspOMI G'GGCC_C
Cuts at: 1355 1525 1355
Size: 170 5907
PstI C_TGCA'G
Cuts at: 676 929 1372 1496 1844 3124 3809 676
Size: 253 443 124 348 1280 685 2944
Fragments arranged by size:
2944 1280 685 443 348 253 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6077
PvuII CAG'CTG
Cuts at: 755 905 1362 1532 3502 3862 755
Size: 150 457 170 1970 360 2970
Fragments arranged by size:
2970 1970 457 360 170 150
RsrII CG'GwC_CG
Cuts at: 4272 4272
Size: 6077
SacI G_AGCT'C
Cuts at: 2036 3091 2036
Size: 1055 5022
SacII CC_GC'GG
Cuts at: 1158 1158
Size: 6077
SalI G'TCGA_C
Cuts at: 202 3114 202
Size: 2912 3165
SapI GCTCTTCn'nnn_
Cuts at: 4100 4310 4100
Size: 210 5867
SbfI CC_TGCA'GG
Cuts at: 1844 3124 1844
Size: 1280 4797
ScaI AGT'ACT
Cuts at: 5594 5594
Size: 6077
SfcI C'TryA_G
Cuts at: 33 399 672 925 1368 1492 1840 1981 3120
Size: 366 273 253 443 124 348 141 1139
Cuts at: 3120 3805 4798 4989 33
Size: 685 993 191 1121
Fragments arranged by size:
1139 1121 993 685 443 366 348 273
253 191 141 124
SfoI GGC'GCC
Cuts at: 1711 2884 3509 3757 1711
Size: 1173 625 248 4031
Fragments arranged by size:
4031 1173 625 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
SmaI CCC'GGG
Cuts at: 392 1130 1277 1354 2244 3099 392
Size: 738 147 77 890 855 3370
Fragments arranged by size:
3370 890 855 738 147 77
SmlI C'TyrA_G
Cuts at: 1684 3232 4147 4639 4901 1684
Size: 1548 915 492 262 2860
Fragments arranged by size:
2860 1548 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6077
SphI G_CATG'C
Cuts at: 3130 3630 3130
Size: 500 5577
SrfI GCCC'GGGC
Cuts at: 2244 2244
Size: 6077
StuI AGG'CCT
Cuts at: 1789 2212 1789
Size: 423 5654
StyI C'CwwG_G
Cuts at: 107 512 618 1247 1733 1937 2999 3211 3565
Size: 405 106 629 486 204 1062 212 354
Cuts at: 3565 107
Size: 2619
Fragments arranged by size:
2619 1062 629 486 405 354 212 204
106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2032 2380 3660 3793 4252 4461 2032
Size: 348 1280 133 459 209 3648
Fragments arranged by size:
3648 1280 459 348 209 133
TatI w'GTAC_w
Cuts at: 2981 5592 2981
Size: 2611 3466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5594 5626 5594
Size: 32 6045
Tth111I GACn'n_nGTC
Cuts at: 3874 3874
Size: 6077
XbaI T'CTAG_A
Cuts at: 3108 3108
Size: 6077
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 2407 3572 657
Size: 1750 1165 3162
Fragments arranged by size:
3162 1750 1165
XmaI C'CCGG_G
Cuts at: 390 1128 1275 1352 2242 3097 390
Size: 738 147 77 890 855 3370
Fragments arranged by size:
3370 890 855 738 147 77
XmnI GAAnn'nnTTC
Cuts at: 2974 2974
Size: 6077
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BbvCI
BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PpuMI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XhoI
ZraI