Restriction map for
pF1KE1066
(Circular) (Six-base) MAPSORT of: pF1KE1066.seq Check: 8204 from: 1 to: 4589
pF1KE1066 4589 bp
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1507 1507
Size: 4589
AccI GT'mk_AC
Cuts at: 302 1627 302
Size: 1325 3264
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 4429 4429
Size: 4589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 758 842 2414 2846 3593 3861 542
Size: 216 84 1572 432 747 268 1270
Fragments arranged by size:
1572 1270 747 432 268 216 84
AfeI AGC'GCT
Cuts at: 232 232
Size: 4589
AflII C'TTAA_G
Cuts at: 361 654 361
Size: 293 4296
AflIII A'CryG_T
Cuts at: 1941 3045 4022 1941
Size: 1104 977 2508
Fragments arranged by size:
2508 1104 977
AgeI A'CCGG_T
Cuts at: 2977 2977
Size: 4589
AhdI GACnn_n'nnGTC
Cuts at: 856 856
Size: 4589
AlwNI CAG_nnn'CTG
Cuts at: 1894 3461 1894
Size: 1567 3022
ApaI G_GGCC'C
Cuts at: 164 1289 164
Size: 1125 3464
ApaLI G'TGCA_C
Cuts at: 3359 3359
Size: 4589
ApoI r'AATT_y
Cuts at: 379 516 1105 1135 1200 1324 1593 3942 4198
Size: 137 589 30 65 124 269 2349 256
Cuts at: 4198 379
Size: 770
Fragments arranged by size:
2349 770 589 269 256 137 124 65
30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4589
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 134 176 1142 1609 134
Size: 42 966 467 3114
Fragments arranged by size:
3114 966 467 42
AvrII C'CTAG_G
Cuts at: 1272 1272
Size: 4589
BamHI G'GATC_C
Cuts at: 1332 1614 1332
Size: 282 4307
BanI G'GyrC_C
Cuts at: 578 1605 2019 2267 2302 578
Size: 1027 414 248 35 2865
Fragments arranged by size:
2865 1027 414 248 35
BanII G_rGCy'C
Cuts at: 135 164 710 1289 1603 2633 135
Size: 29 546 579 314 1030 2091
Fragments arranged by size:
2091 1030 579 546 314 29
BbeI G_GCGC'C
Cuts at: 2023 2271 2023
Size: 248 4341
BbsI GAAGACnn'nnnn_
Cuts at: 764 764
Size: 4589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1526 1560 1526
Size: 34 4555
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3248 4589 2487
Size: 761 1341 2487
Fragments arranged by size:
2487 1341 761
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglII A'GATC_T
Cuts at: 2104 4141 2104
Size: 2037 2552
BlpI GC'TnA_GC
Cuts at: 1701 1701
Size: 4589
Bme1580I G_kGCm'C
Cuts at: 164 1289 1405 2214 2307 3363 164
Size: 1125 116 809 93 1056 1390
Fragments arranged by size:
1390 1125 1056 809 116 93
BmrI ACTGGGnnnn_n'
Cuts at: 113 1419 1446 1801 1882 2217 4361 113
Size: 1306 27 355 81 335 2144 341
Fragments arranged by size:
2144 1306 355 341 335 81 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 729 2956 729
Size: 2227 2362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 783 1367 163
Size: 620 584 3385
Fragments arranged by size:
3385 620 584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1765 2644 3136 3434 1330
Size: 435 879 492 298 2485
Fragments arranged by size:
2485 879 492 435 298
BsaI GGTCTCn'nnnn_
Cuts at: 749 4293 749
Size: 3544 1045
BsaAI yAC'GTr
Cuts at: 219 1942 219
Size: 1723 2866
BsaBI GATnn'nnATC
Cuts at: 6 1076 2103 4140 6
Size: 1070 1027 2037 455
Fragments arranged by size:
2037 1070 1027 455
BsaHI Gr'CG_yC
Cuts at: 112 1489 2020 2268 2970 4466 112
Size: 1377 531 248 702 1496 235
Fragments arranged by size:
1496 1377 702 531 248 235
BsaWI w'CCGG_w
Cuts at: 1778 2299 2977 3251 3398 3809 1778
Size: 521 678 274 147 411 2558
Fragments arranged by size:
2558 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 199
Size: 30 4559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 973 1327 1521 973
Size: 354 194 4041
Fragments arranged by size:
4041 354 194
BseYI C'CCAG_C
Cuts at: 950 2014 2772 3349 3845 950
Size: 1064 758 577 496 1694
Fragments arranged by size:
1694 1064 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 1535
Size: 4589
BsiEI CG_ry'CG
Cuts at: 104 169 315 321 2131 2177 3385 104
Size: 65 146 6 1810 46 1208 1308
Fragments arranged by size:
1810 1308 1208 146 65 46 6
BsiHKAI G_wGCw'C
Cuts at: 573 710 1483 1603 2381 2571 3363 573
Size: 137 773 120 778 190 792 1799
Fragments arranged by size:
1799 792 778 773 190 137 120
BsmI GAATG_Cn'
Cuts at: 1167 1167
Size: 4589
BsmBI CGTCTCn'nnnn_
Cuts at: 119 119
Size: 4589
Bsp1286I G_dGCh'C
Cuts at: 135 164 573 710 1289 1405 1483 1603 2214
Size: 29 409 137 579 116 78 120 611
Cuts at: 2214 2307 2381 2571 2633 3363 135
Size: 93 74 190 62 730 1361
Fragments arranged by size:
1361 730 611 579 409 190 137 120
116 93 78 74 62 29
BspEI T'CCGG_A
Cuts at: 3809 3809
Size: 4589
BspHI T'CATG_A
Cuts at: 3765 3765
Size: 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 277 1268 1507 1639 1853 2155 2536 277
Size: 991 239 132 214 302 381 2330
Fragments arranged by size:
2330 991 381 302 239 214 132
BsrBI CCG'CTC
Cuts at: 50 171 290 1364 2881 2935 4419 4584 50
Size: 121 119 1074 1517 54 1484 165 55
Fragments arranged by size:
1517 1484 1074 165 121 119 55 54
BsrDI GCAATG_nn'
Cuts at: 2501 2501
Size: 4589
BsrFI r'CCGG_y
Cuts at: 257 2587 2768 2977 257
Size: 2330 181 209 1869
Fragments arranged by size:
2330 1869 209 181
BssSI C'ACGA_G
Cuts at: 2860 3218 2860
Size: 358 4231
BstAPI GCAn_nnn'nTGC
Cuts at: 1966 1966
Size: 4589
BstBI TT'CG_AA
Cuts at: 2950 2950
Size: 4589
BstEII G'GTnAC_C
Cuts at: 625 1548 625
Size: 923 3666
BstYI r'GATC_y
Cuts at: 1 1332 1614 2081 2104 2439 2685 3697 3783
Size: 1331 282 467 23 335 246 1012 86
Cuts at: 3783 3795 4141 1
Size: 12 346 449
Fragments arranged by size:
1331 1012 467 449 346 335 282 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1231 1231
Size: 4589
BtgI C'CryG_G
Cuts at: 107 194 1319 107
Size: 87 1125 3377
Fragments arranged by size:
3377 1125 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1397 1485 2539 2720 2883 1397
Size: 88 1054 181 163 3103
Fragments arranged by size:
3103 1054 181 163 88
BtsI GCAGTG_nn'
Cuts at: 1154 1219 1518 1832 1154
Size: 65 299 314 3911
Fragments arranged by size:
3911 314 299 65
DraI TTT'AAA
Cuts at: 1563 1588 1563
Size: 25 4564
DrdI GACnn_nn'nnGTC
Cuts at: 2295 3153 2295
Size: 858 3731
EaeI y'GGCC_r
Cuts at: 166 312 2174 2348 2739 2766 4111 166
Size: 146 1862 174 391 27 1345 644
Fragments arranged by size:
1862 1345 644 391 174 146 27
EagI C'GGCC_G
Cuts at: 166 312 2174 166
Size: 146 1862 2581
Fragments arranged by size:
2581 1862 146
EarI CTCTTCn'nnn_
Cuts at: 2612 2822 2612
Size: 210 4379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2797 3107 3253 4399 2797
Size: 310 146 1146 2987
Fragments arranged by size:
2987 1146 310 146
EcoICRI GAG'CTC
Cuts at: 708 1601 708
Size: 893 3696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 542 758 783 842 1367 2414 2846 3593
Size: 379 216 25 59 525 1047 432 747
Cuts at: 3593 3861 163
Size: 268 891
Fragments arranged by size:
1047 891 747 525 432 379 268 216
59 25
EcoO109I rG'GnC_Cy
Cuts at: 161 180 901 979 1285 1286 1728 161
Size: 19 721 78 306 1 442 3022
Fragments arranged by size:
3022 721 442 306 78 19 1
EcoRI G'AATT_C
Cuts at: 1593 1593
Size: 4589
EcoRV GAT'ATC
Cuts at: 1443 1443
Size: 4589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4589
FspI TGC'GCA
Cuts at: 2370 2370
Size: 4589
HaeII r_GCGC'y
Cuts at: 142 234 2023 2271 3293 4272 142
Size: 92 1789 248 1022 979 459
Fragments arranged by size:
1789 1022 979 459 248 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 411 443 597 629 842 874 2431 2461 2463
Size: 32 154 32 213 32 1557 30 2
Cuts at: 2463 2493 411
Size: 30 2507
Fragments arranged by size:
2507 1557 213 154 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1050 1628 1050
Size: 578 4011
HindIII A'AGCT_T
Cuts at: 556 814 1565 556
Size: 258 751 3580
Fragments arranged by size:
3580 751 258
KasI G'GCGC_C
Cuts at: 2019 2267 2019
Size: 248 4341
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MluI A'CGCG_T
Cuts at: 4022 4022
Size: 4589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3260 3444 3260
Size: 184 4405
MscI TGG'CCA
Cuts at: 2350 2350
Size: 4589
MslI CAynn'nnrTG
Cuts at: 2578 2705 2578
Size: 127 4462
MspA1I CmG'CkG
Cuts at: 240 950 1044 1700 2014 2374 3387 3632 240
Size: 710 94 656 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 710 656 360 314 245 94
NaeI GCC'GGC
Cuts at: 259 2770 259
Size: 2511 2078
NarI GG'CG_CC
Cuts at: 2020 2268 2020
Size: 248 4341
NcoI C'CATG_G
Cuts at: 107 1319 107
Size: 1212 3377
NgoMIV G'CCGG_C
Cuts at: 257 2768 257
Size: 2511 2078
NruI TCG'CGA
Cuts at: 1504 1504
Size: 4589
NspI r_CATG'y
Cuts at: 1198 1642 2142 3049 1198
Size: 444 500 907 2738
Fragments arranged by size:
2738 907 500 444
PasI CC'CwG_GG
Cuts at: 975 975
Size: 4589
PciI A'CATG_T
Cuts at: 3045 3045
Size: 4589
PflMI CCAn_nnn'nTGG
Cuts at: 1458 1458
Size: 4589
PfoI T'CCnGG_A
Cuts at: 1335 1335
Size: 4589
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4557
PpuMI rG'GwC_Cy
Cuts at: 180 180
Size: 4589
PspOMI G'GGCC_C
Cuts at: 160 1285 160
Size: 1125 3464
PspXI vC'TCGA_Gb
Cuts at: 1142 1142
Size: 4589
PstI C_TGCA'G
Cuts at: 1539 1636 2321 1539
Size: 97 685 3807
Fragments arranged by size:
3807 685 97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 950 1044 2014 2374 950
Size: 94 970 360 3165
Fragments arranged by size:
3165 970 360 94
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 4589
SacI G_AGCT'C
Cuts at: 710 1603 710
Size: 893 3696
SalI G'TCGA_C
Cuts at: 1626 1626
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 2612 2822 2612
Size: 210 4379
SbfI CC_TGCA'GG
Cuts at: 1636 1636
Size: 4589
ScaI AGT'ACT
Cuts at: 4106 4106
Size: 4589
SexAI A'CCwGG_T
Cuts at: 182 629 1247 182
Size: 447 618 3524
Fragments arranged by size:
3524 618 447
SfcI C'TryA_G
Cuts at: 33 945 1535 1632 2317 3310 3501 33
Size: 912 590 97 685 993 191 1121
Fragments arranged by size:
1121 993 912 685 590 191 97
SfoI GGC'GCC
Cuts at: 2021 2269 2021
Size: 248 4341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SgrAI Cr'CCGG_yG
Cuts at: 257 257
Size: 4589
SmaI CCC'GGG
Cuts at: 136 178 1611 136
Size: 42 1433 3114
Fragments arranged by size:
3114 1433 42
SmlI C'TyrA_G
Cuts at: 361 654 1142 1309 1744 2659 3151 3413 361
Size: 293 488 167 435 915 492 262 1537
Fragments arranged by size:
1537 915 492 488 435 293 262 167
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 1642 2142 1642
Size: 500 4089
SrfI GCCC'GGGC
Cuts at: 136 136
Size: 4589
SspI AAT'ATT
Cuts at: 1170 1170
Size: 4589
StuI AGG'CCT
Cuts at: 527 527
Size: 4589
StyI C'CwwG_G
Cuts at: 107 539 904 1272 1319 1723 2077 107
Size: 432 365 368 47 404 354 2619
Fragments arranged by size:
2619 432 404 368 365 354 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 942 1498 1512 2172 2305 2764 2973 942
Size: 556 14 660 133 459 209 2558
Fragments arranged by size:
2558 660 556 459 209 133 14
TatI w'GTAC_w
Cuts at: 4104 4104
Size: 4589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4557
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 1620 1620
Size: 4589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1326 2084 1326
Size: 758 3831
XhoI C'TCGA_G
Cuts at: 1142 1142
Size: 4589
XmaI C'CCGG_G
Cuts at: 134 176 1609 134
Size: 42 1433 3114
Fragments arranged by size:
3114 1433 42
XmnI GAAnn'nnTTC
Cuts at: 380 527 380
Size: 147 4442
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI AloI AscI BaeI BbvCI BfrBI
BglI BmgBI BmtI Bpu10I BsiWI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NsiI PacI PmlI PshAI
PsiI PsrI SacII SanDI SfiI SnaBI SwaI ZraI