Restriction map for
pF1KE1068
(Circular) (Six-base) MAPSORT of: pF1KE1068.seq Check: 5754 from: 1 to: 4844
pF1KE1068 4844 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 191 1509 1552 1882 191
Size: 1318 43 330 3153
Fragments arranged by size:
3153 1318 330 43
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 544 797 1207 1243 1814 2669 3101 3848
Size: 311 253 410 36 571 855 432 747
Cuts at: 3848 4116 233
Size: 268 961
Fragments arranged by size:
961 855 747 571 432 410 311 268
253 36
AfeI AGC'GCT
Cuts at: 1696 1696
Size: 4844
AflII C'TTAA_G
Cuts at: 268 1000 268
Size: 732 4112
AflIII A'CryG_T
Cuts at: 910 1504 2196 3300 4277 910
Size: 594 692 1104 977 1477
Fragments arranged by size:
1477 1104 977 692 594
AgeI A'CCGG_T
Cuts at: 3232 3232
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 1215 1266 1665 1818 2149 3716 1215
Size: 51 399 153 331 1567 2343
Fragments arranged by size:
2343 1567 399 331 153 51
ApaI G_GGCC'C
Cuts at: 127 127
Size: 4844
ApaLI G'TGCA_C
Cuts at: 3614 3614
Size: 4844
ApoI r'AATT_y
Cuts at: 203 345 1848 4197 4453 203
Size: 142 1503 2349 256 594
Fragments arranged by size:
2349 1503 594 256 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 1864 1864
Size: 4844
BamHI G'GATC_C
Cuts at: 1869 1869
Size: 4844
BanI G'GyrC_C
Cuts at: 1860 2274 2522 2557 1860
Size: 414 248 35 4147
Fragments arranged by size:
4147 414 248 35
BanII G_rGCy'C
Cuts at: 127 1672 1858 2888 127
Size: 1545 186 1030 2083
Fragments arranged by size:
2083 1545 1030 186
BbeI G_GCGC'C
Cuts at: 2278 2526 2278
Size: 248 4596
BbsI GAAGACnn'nnnn_
Cuts at: 404 1447 404
Size: 1043 3801
BbvCI CC'TCA_GC
Cuts at: 1691 1691
Size: 4844
BciVI GTATCCnnnnn_n'
Cuts at: 696 2742 3503 4844 696
Size: 2046 761 1341 696
Fragments arranged by size:
2046 1341 761 696
BclI T'GATC_A
Cuts at: 536 2364 536
Size: 1828 3016
BglII A'GATC_T
Cuts at: 1190 2359 4396 1190
Size: 1169 2037 1638
Fragments arranged by size:
2037 1638 1169
BlpI GC'TnA_GC
Cuts at: 482 1956 482
Size: 1474 3370
Bme1580I G_kGCm'C
Cuts at: 127 1161 2469 2562 3618 127
Size: 1034 1308 93 1056 1353
Fragments arranged by size:
1353 1308 1056 1034 93
BmgBI CAC'GTC
Cuts at: 117 117
Size: 4844
BmrI ACTGGGnnnn_n'
Cuts at: 131 1473 2056 2137 2472 4616 131
Size: 1342 583 81 335 2144 359
Fragments arranged by size:
2144 1342 583 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 4844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 691 911 1689 174
Size: 517 220 778 3329
Fragments arranged by size:
3329 778 517 220
Bpu10I CC'TnA_GC
Cuts at: 1691 1691
Size: 4844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 2020 2899 3391 3689 762
Size: 1258 879 492 298 1917
Fragments arranged by size:
1917 1258 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 895 4548 895
Size: 3653 1191
BsaAI yAC'GTr
Cuts at: 2197 2197
Size: 4844
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 2275 2523 3225 4721 2275
Size: 248 702 1496 2398
Fragments arranged by size:
2398 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2033 2554 3232 3506 3653 4064 2033
Size: 521 678 274 147 411 2813
Fragments arranged by size:
2813 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1609 1639 1609
Size: 30 4814
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1819 1819
Size: 4844
BseYI C'CCAG_C
Cuts at: 1274 1714 2269 3027 3604 4100 1274
Size: 440 555 758 577 496 2018
Fragments arranged by size:
2018 758 577 555 496 440
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3640 104
Size: 2282 46 1208 1308
Fragments arranged by size:
2282 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1858 2636 2826 3618 1858
Size: 778 190 792 3084
Fragments arranged by size:
3084 792 778 190
BsmI GAATG_Cn'
Cuts at: 583 674 583
Size: 91 4753
Bsp1286I G_dGCh'C
Cuts at: 127 1161 1672 1858 2469 2562 2636 2826 2888
Size: 1034 511 186 611 93 74 190 62
Cuts at: 2888 3618 127
Size: 730 1353
Fragments arranged by size:
1353 1034 730 611 511 190 186 93
74 62
BspEI T'CCGG_A
Cuts at: 4064 4064
Size: 4844
BspHI T'CATG_A
Cuts at: 1053 4020 1053
Size: 2967 1877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 672 948 1894 2108 2410 2791 672
Size: 276 946 214 302 381 2725
Fragments arranged by size:
2725 946 381 302 276 214
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 410 2756 410
Size: 2346 2498
BsrFI r'CCGG_y
Cuts at: 2842 3023 3232 2842
Size: 181 209 4454
Fragments arranged by size:
4454 209 181
BssSI C'ACGA_G
Cuts at: 3115 3473 3115
Size: 358 4486
BstAPI GCAn_nnn'nTGC
Cuts at: 1218 2221 1218
Size: 1003 3841
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstYI r'GATC_y
Cuts at: 1 1190 1869 2336 2359 2694 2940 3952 4038
Size: 1189 679 467 23 335 246 1012 86
Cuts at: 4038 4050 4396 1
Size: 12 346 449
Fragments arranged by size:
1189 1012 679 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 192 192
Size: 4844
BtgI C'CryG_G
Cuts at: 107 143 302 107
Size: 36 159 4649
Fragments arranged by size:
4649 159 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 2975 3138 2794
Size: 181 163 4500
Fragments arranged by size:
4500 181 163
BtsI GCAGTG_nn'
Cuts at: 1159 2087 1159
Size: 928 3916
DraI TTT'AAA
Cuts at: 1843 1843
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3408 2550
Size: 858 3986
EaeI y'GGCC_r
Cuts at: 434 2429 2603 2994 3021 4366 434
Size: 1995 174 391 27 1345 912
Fragments arranged by size:
1995 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 563 1790 2867 3077 563
Size: 1227 1077 210 2330
Fragments arranged by size:
2330 1227 1077 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3052 3362 3508 4654 127
Size: 2925 310 146 1146 317
Fragments arranged by size:
2925 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 233 544 691 797 911 1207 1243 1689
Size: 59 311 147 106 114 296 36 446
Cuts at: 1689 1814 2669 3101 3848 4116 174
Size: 125 855 432 747 268 902
Fragments arranged by size:
902 855 747 446 432 311 296 268
147 125 114 106 59 36
EcoO109I rG'GnC_Cy
Cuts at: 124 1718 1983 124
Size: 1594 265 2985
Fragments arranged by size:
2985 1594 265
EcoRI G'AATT_C
Cuts at: 345 1848 345
Size: 1503 3341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4844
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 1257 1698 2278 2526 3548 4527 1257
Size: 441 580 248 1022 979 1574
Fragments arranged by size:
1574 1022 979 580 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 753 785 2686 2716 2718 2748 475
Size: 32 246 32 1901 30 2 30 2571
Fragments arranged by size:
2571 1901 246 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1883 1883
Size: 4844
HindIII A'AGCT_T
Cuts at: 1288 1288
Size: 4844
KasI G'GCGC_C
Cuts at: 2274 2522 2274
Size: 248 4596
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MfeI C'AATT_G
Cuts at: 1335 1335
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 1628 1764 1781 3515 3699 497
Size: 1131 136 17 1734 184 1642
Fragments arranged by size:
1734 1642 1131 184 136 17
MscI TGG'CCA
Cuts at: 436 2605 436
Size: 2169 2675
MslI CAynn'nnrTG
Cuts at: 2833 2960 2833
Size: 127 4717
MspA1I CmG'CkG
Cuts at: 145 1955 2269 2629 3642 3887 145
Size: 1810 314 360 1013 245 1102
Fragments arranged by size:
1810 1102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 2275 2523 2275
Size: 248 4596
NcoI C'CATG_G
Cuts at: 107 302 107
Size: 195 4649
NdeI CA'TA_TG
Cuts at: 1463 1463
Size: 4844
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NspI r_CATG'y
Cuts at: 914 1508 1897 2397 3304 914
Size: 594 389 500 907 2454
Fragments arranged by size:
2454 907 594 500 389
PacI TTA_AT'TAA
Cuts at: 425 425
Size: 4844
PciI A'CATG_T
Cuts at: 910 1504 3300 910
Size: 594 1796 2454
Fragments arranged by size:
2454 1796 594
PflMI CCAn_nnn'nTGG
Cuts at: 1417 1417
Size: 4844
PfoI T'CCnGG_A
Cuts at: 198 800 198
Size: 602 4242
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4812
PshAI GACnn'nnGTC
Cuts at: 789 789
Size: 4844
PspOMI G'GGCC_C
Cuts at: 123 123
Size: 4844
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 179 211 179
Size: 32 4812
PstI C_TGCA'G
Cuts at: 355 798 945 1044 1891 2576 355
Size: 443 147 99 847 685 2623
Fragments arranged by size:
2623 847 685 443 147 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 2269 2629 2269
Size: 360 4484
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SacII CC_GC'GG
Cuts at: 146 146
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 563 2867 3077 563
Size: 2304 210 2330
Fragments arranged by size:
2330 2304 210
SbfI CC_TGCA'GG
Cuts at: 355 1891 355
Size: 1536 3308
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SfcI C'TryA_G
Cuts at: 33 351 565 794 941 1040 1887 2572 3565
Size: 318 214 229 147 99 847 685 993
Cuts at: 3565 3756 33
Size: 191 1121
Fragments arranged by size:
1121 993 847 685 318 229 214 191
147 99
SfoI GGC'GCC
Cuts at: 2276 2524 2276
Size: 248 4596
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1866 1866
Size: 4844
SmlI C'TyrA_G
Cuts at: 268 741 1000 1999 2914 3406 3668 268
Size: 473 259 999 915 492 262 1444
Fragments arranged by size:
1444 999 915 492 473 262 259
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
SspI AAT'ATT
Cuts at: 610 610
Size: 4844
StyI C'CwwG_G
Cuts at: 107 302 1978 2332 107
Size: 195 1676 354 2619
Fragments arranged by size:
2619 1676 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2427 2560 3019 3228 2427
Size: 133 459 209 4043
Fragments arranged by size:
4043 459 209 133
TatI w'GTAC_w
Cuts at: 4359 4359
Size: 4844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1609 1641 4361 4393 1609
Size: 32 2720 32 2060
Fragments arranged by size:
2720 2060 32 32
Tth111I GACn'n_nGTC
Cuts at: 1745 2641 1745
Size: 896 3948
XbaI T'CTAG_A
Cuts at: 882 1875 882
Size: 993 3851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2339 114
Size: 2225 2619
XmaI C'CCGG_G
Cuts at: 1864 1864
Size: 4844
XmnI GAAnn'nnTTC
Cuts at: 557 557
Size: 4844
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PacI PciI PflMI PfoI PmeI PpiI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NheI NotI NruI
NsiI PasI PmlI PpuMI PsiI PspXI SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI