Restriction map for
pF1KE1073
(Circular) (Six-base) MAPSORT of: pF1KE1073.seq Check: 3700 from: 1 to: 5546
pF1KE1073 5546 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 173 219 428 2584 173
Size: 46 209 2156 3135
Fragments arranged by size:
3135 2156 209 46
Acc65I G'GTAC_C
Cuts at: 2562 2562
Size: 5546
AclI AA'CG_TT
Cuts at: 5386 5386
Size: 5546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 658 689 877 974 2368 2443 3371 3803
Size: 263 31 188 97 1394 75 928 432
Cuts at: 3803 4550 4818 395
Size: 747 268 1123
Fragments arranged by size:
1394 1123 928 747 432 268 263 188
97 75 31
AflII C'TTAA_G
Cuts at: 1882 1882
Size: 5546
AflIII A'CryG_T
Cuts at: 2898 4002 4979 2898
Size: 1104 977 3465
Fragments arranged by size:
3465 1104 977
AgeI A'CCGG_T
Cuts at: 3934 3934
Size: 5546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 251 251
Size: 5546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1157 1189 1157
Size: 32 5514
AlwNI CAG_nnn'CTG
Cuts at: 1927 2851 4418 1927
Size: 924 1567 3055
Fragments arranged by size:
3055 1567 924
ApaLI G'TGCA_C
Cuts at: 1520 4316 1520
Size: 2796 2750
ApoI r'AATT_y
Cuts at: 395 1805 2550 4899 5155 395
Size: 1410 745 2349 256 786
Fragments arranged by size:
2349 1410 786 745 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
AvaI C'yCGr_G
Cuts at: 1796 2566 1796
Size: 770 4776
BamHI G'GATC_C
Cuts at: 2224 2571 2224
Size: 347 5199
BanI G'GyrC_C
Cuts at: 2562 2976 3224 3259 2562
Size: 414 248 35 4849
Fragments arranged by size:
4849 414 248 35
BanII G_rGCy'C
Cuts at: 188 1175 2402 2560 3590 188
Size: 987 1227 158 1030 2144
Fragments arranged by size:
2144 1227 1030 987 158
BbeI G_GCGC'C
Cuts at: 2980 3228 2980
Size: 248 5298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5512
BciVI GTATCCnnnnn_n'
Cuts at: 3444 4205 5546 3444
Size: 761 1341 3444
Fragments arranged by size:
3444 1341 761
BclI T'GATC_A
Cuts at: 288 3066 288
Size: 2778 2768
BfrBI ATG'CAT
Cuts at: 2059 2059
Size: 5546
BglII A'GATC_T
Cuts at: 3061 5098 3061
Size: 2037 3509
BlpI GC'TnA_GC
Cuts at: 1898 2658 1898
Size: 760 4786
Bme1580I G_kGCm'C
Cuts at: 1524 1797 3171 3264 4320 1524
Size: 273 1374 93 1056 2750
Fragments arranged by size:
2750 1374 1056 273 93
BmgBI CAC'GTC
Cuts at: 432 432
Size: 5546
BmrI ACTGGGnnnn_n'
Cuts at: 2758 2839 3174 5318 2758
Size: 81 335 2144 2986
Fragments arranged by size:
2986 2144 335 81
BmtI G_CTAG'C
Cuts at: 1594 1594
Size: 5546
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3913 3913
Size: 5546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2522 1159
Size: 1363 4183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2230 2722 3601 4093 4391 1924
Size: 306 492 879 492 298 3079
Fragments arranged by size:
3079 879 492 492 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 5250 5250
Size: 5546
BsaAI yAC'GTr
Cuts at: 2514 2899 2514
Size: 385 5161
BsaBI GATnn'nnATC
Cuts at: 6 3060 5097 6
Size: 3054 2037 455
Fragments arranged by size:
3054 2037 455
BsaHI Gr'CG_yC
Cuts at: 2977 3225 3927 5423 2977
Size: 248 702 1496 3100
Fragments arranged by size:
3100 1496 702 248
BsaWI w'CCGG_w
Cuts at: 403 2735 3256 3934 4208 4355 4766 403
Size: 2332 521 678 274 147 411 1183
Fragments arranged by size:
2332 1183 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 416 446 1157 1187 416
Size: 30 711 30 4775
Fragments arranged by size:
4775 711 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1762 1762
Size: 5546
BseYI C'CCAG_C
Cuts at: 2971 3729 4306 4802 2971
Size: 758 577 496 3715
Fragments arranged by size:
3715 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1257 2326 1257
Size: 1069 4477
BsiEI CG_ry'CG
Cuts at: 104 201 3088 3134 4342 104
Size: 97 2887 46 1208 1308
Fragments arranged by size:
2887 1308 1208 97 46
BsiHKAI G_wGCw'C
Cuts at: 1175 1247 1524 2402 2560 3338 3528 4320 1175
Size: 72 277 878 158 778 190 792 2401
Fragments arranged by size:
2401 878 792 778 277 190 158 72
BsmI GAATG_Cn'
Cuts at: 1303 2061 2077 1303
Size: 758 16 4772
Fragments arranged by size:
4772 758 16
Bsp1286I G_dGCh'C
Cuts at: 188 1175 1247 1524 1797 2402 2560 3171 3264
Size: 987 72 277 273 605 158 611 93
Cuts at: 3264 3338 3528 3590 4320 188
Size: 74 190 62 730 1414
Fragments arranged by size:
1414 987 730 611 605 277 273 190
158 93 74 72 62
BspEI T'CCGG_A
Cuts at: 4766 4766
Size: 5546
BspHI T'CATG_A
Cuts at: 4722 4722
Size: 5546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2596 2810 3112 3493 2596
Size: 214 302 381 4649
Fragments arranged by size:
4649 381 302 214
BsrBI CCG'CTC
Cuts at: 50 203 521 1848 1908 3838 3892 5376 5541
Size: 153 318 1327 60 1930 54 1484 165
Cuts at: 5541 50
Size: 55
Fragments arranged by size:
1930 1484 1327 318 165 153 60 55
54
BsrDI GCAATG_nn'
Cuts at: 1859 3458 1859
Size: 1599 3947
BsrFI r'CCGG_y
Cuts at: 3544 3725 3934 3544
Size: 181 209 5156
Fragments arranged by size:
5156 209 181
BssSI C'ACGA_G
Cuts at: 3817 4175 3817
Size: 358 5188
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 2923 1837
Size: 1086 4460
BstBI TT'CG_AA
Cuts at: 1563 3907 1563
Size: 2344 3202
BstYI r'GATC_y
Cuts at: 1 2224 2571 3038 3061 3396 3642 4654 4740
Size: 2223 347 467 23 335 246 1012 86
Cuts at: 4740 4752 5098 1
Size: 12 346 449
Fragments arranged by size:
2223 1012 467 449 347 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 880 880
Size: 5546
BtgI C'CryG_G
Cuts at: 459 459
Size: 5546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3496 3677 3840 3496
Size: 181 163 5202
Fragments arranged by size:
5202 181 163
BtsI GCAGTG_nn'
Cuts at: 1437 2789 1437
Size: 1352 4194
ClaI AT'CG_AT
Cuts at: 1727 1727
Size: 5546
DraI TTT'AAA
Cuts at: 1502 2264 2545 1502
Size: 762 281 4503
Fragments arranged by size:
4503 762 281
DraIII CAC_nnn'GTG
Cuts at: 391 391
Size: 5546
DrdI GACnn_nn'nnGTC
Cuts at: 3252 4110 3252
Size: 858 4688
EaeI y'GGCC_r
Cuts at: 122 198 2525 3131 3305 3696 3723 5068 122
Size: 76 2327 606 174 391 27 1345 600
Fragments arranged by size:
2327 1345 606 600 391 174 76 27
EagI C'GGCC_G
Cuts at: 198 3131 198
Size: 2933 2613
EarI CTCTTCn'nnn_
Cuts at: 1871 2276 3569 3779 1871
Size: 405 1293 210 3638
Fragments arranged by size:
3638 1293 405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1929 3754 4064 4210 5356 1929
Size: 1825 310 146 1146 2119
Fragments arranged by size:
2119 1825 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1173 2400 2558 1173
Size: 1227 158 4161
Fragments arranged by size:
4161 1227 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 658 689 877 974 1159 2368 2443 2522
Size: 263 31 188 97 185 1209 75 79
Cuts at: 2522 3371 3803 4550 4818 395
Size: 849 432 747 268 1123
Fragments arranged by size:
1209 1123 849 747 432 268 263 188
185 97 79 75 31
EcoO109I rG'GnC_Cy
Cuts at: 2685 2685
Size: 5546
EcoRI G'AATT_C
Cuts at: 2550 2550
Size: 5546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1897 3565 1513
Size: 384 1668 3494
Fragments arranged by size:
3494 1668 384
FspI TGC'GCA
Cuts at: 3327 3327
Size: 5546
HaeII r_GCGC'y
Cuts at: 2980 3228 4250 5229 2980
Size: 248 1022 979 3297
Fragments arranged by size:
3297 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 348 380 3388 3418 3420 3450 348
Size: 32 3008 30 2 30 2444
Fragments arranged by size:
3008 2444 32 30 30 2
HincII GTy'rAC
Cuts at: 220 558 1981 2585 220
Size: 338 1423 604 3181
Fragments arranged by size:
3181 1423 604 338
HindIII A'AGCT_T
Cuts at: 1190 1354 1190
Size: 164 5382
KasI G'GCGC_C
Cuts at: 2976 3224 2976
Size: 248 5298
KpnI G_GTAC'C
Cuts at: 2566 2566
Size: 5546
MfeI C'AATT_G
Cuts at: 1708 1708
Size: 5546
MluI A'CGCG_T
Cuts at: 4979 4979
Size: 5546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 632 1125 4217 4401 632
Size: 493 3092 184 1777
Fragments arranged by size:
3092 1777 493 184
MscI TGG'CCA
Cuts at: 124 2527 3307 124
Size: 2403 780 2363
Fragments arranged by size:
2403 2363 780
MslI CAynn'nnrTG
Cuts at: 854 3535 3662 854
Size: 2681 127 2738
Fragments arranged by size:
2738 2681 127
MspA1I CmG'CkG
Cuts at: 215 678 2257 2657 2971 3331 4344 4589 215
Size: 463 1579 400 314 360 1013 245 1172
Fragments arranged by size:
1579 1172 1013 463 400 360 314 245
NaeI GCC'GGC
Cuts at: 3727 3727
Size: 5546
NarI GG'CG_CC
Cuts at: 2977 3225 2977
Size: 248 5298
NcoI C'CATG_G
Cuts at: 459 459
Size: 5546
NgoMIV G'CCGG_C
Cuts at: 3725 3725
Size: 5546
NheI G'CTAG_C
Cuts at: 1590 1590
Size: 5546
NsiI A_TGCA'T
Cuts at: 2061 2061
Size: 5546
NspI r_CATG'y
Cuts at: 788 2599 3099 4006 788
Size: 1811 500 907 2328
Fragments arranged by size:
2328 1811 907 500
PciI A'CATG_T
Cuts at: 4002 4002
Size: 5546
PfoI T'CCnGG_A
Cuts at: 1742 1742
Size: 5546
PmeI GTTT'AAAC
Cuts at: 2545 2545
Size: 5546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1157 1189 4685 4717 1157
Size: 32 3496 32 1986
Fragments arranged by size:
3496 1986 32 32
PsiI TTA'TAA
Cuts at: 346 346
Size: 5546
PstI C_TGCA'G
Cuts at: 2593 3278 2593
Size: 685 4861
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5546
PvuII CAG'CTG
Cuts at: 2257 2971 3331 2257
Size: 714 360 4472
Fragments arranged by size:
4472 714 360
RsrII CG'GwC_CG
Cuts at: 3741 3741
Size: 5546
SacI G_AGCT'C
Cuts at: 1175 2402 2560 1175
Size: 1227 158 4161
Fragments arranged by size:
4161 1227 158
SalI G'TCGA_C
Cuts at: 218 2583 218
Size: 2365 3181
SapI GCTCTTCn'nnn_
Cuts at: 1871 3569 3779 1871
Size: 1698 210 3638
Fragments arranged by size:
3638 1698 210
SbfI CC_TGCA'GG
Cuts at: 2593 2593
Size: 5546
ScaI AGT'ACT
Cuts at: 1773 5063 1773
Size: 3290 2256
SfcI C'TryA_G
Cuts at: 33 622 1442 2589 3274 4267 4458 33
Size: 589 820 1147 685 993 191 1121
Fragments arranged by size:
1147 1121 993 820 685 589 191
SfoI GGC'GCC
Cuts at: 2978 3226 2978
Size: 248 5298
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
SmaI CCC'GGG
Cuts at: 2568 2568
Size: 5546
SmlI C'TyrA_G
Cuts at: 1882 1903 2209 2701 3616 4108 4370 1882
Size: 21 306 492 915 492 262 3058
Fragments arranged by size:
3058 915 492 492 306 262 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5546
SphI G_CATG'C
Cuts at: 788 2599 3099 788
Size: 1811 500 3235
Fragments arranged by size:
3235 1811 500
StuI AGG'CCT
Cuts at: 631 631
Size: 5546
StyI C'CwwG_G
Cuts at: 119 459 2680 3034 119
Size: 340 2221 354 2631
Fragments arranged by size:
2631 2221 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 452 709 3129 3262 3721 3930 452
Size: 257 2420 133 459 209 2068
Fragments arranged by size:
2420 2068 459 257 209 133
TatI w'GTAC_w
Cuts at: 587 1771 2334 5061 587
Size: 1184 563 2727 1072
Fragments arranged by size:
2727 1184 1072 563
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 416 448 5063 5095 416
Size: 32 4615 32 867
Fragments arranged by size:
4615 867 32 32
Tth111I GACn'n_nGTC
Cuts at: 3343 3343
Size: 5546
XbaI T'CTAG_A
Cuts at: 2577 2577
Size: 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3041 3041
Size: 5546
XmaI C'CCGG_G
Cuts at: 2566 2566
Size: 5546
XmnI GAAnn'nnTTC
Cuts at: 921 921
Size: 5546
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI AvrII
BaeI BbsI BbvCI BglI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I CspCI EcoNI EcoRV FseI
FspAI HpaI NdeI NotI NruI PacI PasI PflMI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI