Restriction map for
pF1KE1074
(Circular) (Six-base) MAPSORT of: pF1KE1074.seq Check: 5262 from: 1 to: 5243
pF1KE1074 5243 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 1514 2281 1514
Size: 767 4476
Acc65I G'GTAC_C
Cuts at: 2259 2259
Size: 5243
AclI AA'CG_TT
Cuts at: 5083 5083
Size: 5243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3500 4247 4515 3068
Size: 432 747 268 3796
Fragments arranged by size:
3796 747 432 268
AfeI AGC'GCT
Cuts at: 709 709
Size: 5243
AflII C'TTAA_G
Cuts at: 1081 1081
Size: 5243
AflIII A'CryG_T
Cuts at: 1148 2595 3699 4676 1148
Size: 1447 1104 977 1715
Fragments arranged by size:
1715 1447 1104 977
AgeI A'CCGG_T
Cuts at: 3631 3631
Size: 5243
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2111 2111
Size: 5243
AlwNI CAG_nnn'CTG
Cuts at: 1665 2548 4115 1665
Size: 883 1567 2793
Fragments arranged by size:
2793 1567 883
ApaLI G'TGCA_C
Cuts at: 1894 4013 1894
Size: 2119 3124
ApoI r'AATT_y
Cuts at: 2247 4596 4852 2247
Size: 2349 256 2638
Fragments arranged by size:
2638 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5243
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
AvaI C'yCGr_G
Cuts at: 2263 2263
Size: 5243
BamHI G'GATC_C
Cuts at: 1446 2268 1446
Size: 822 4421
BanI G'GyrC_C
Cuts at: 2259 2673 2921 2956 2259
Size: 414 248 35 4546
Fragments arranged by size:
4546 414 248 35
BanII G_rGCy'C
Cuts at: 175 2257 3287 175
Size: 2082 1030 2131
Fragments arranged by size:
2131 2082 1030
BbeI G_GCGC'C
Cuts at: 2677 2925 2677
Size: 248 4995
BbsI GAAGACnn'nnnn_
Cuts at: 805 1020 2182 805
Size: 215 1162 3866
Fragments arranged by size:
3866 1162 215
BbvCI CC'TCA_GC
Cuts at: 871 871
Size: 5243
BciVI GTATCCnnnnn_n'
Cuts at: 1731 1971 3141 3902 5243 1731
Size: 240 1170 761 1341 1731
Fragments arranged by size:
1731 1341 1170 761 240
BclI T'GATC_A
Cuts at: 999 1253 2763 999
Size: 254 1510 3479
Fragments arranged by size:
3479 1510 254
BfrBI ATG'CAT
Cuts at: 349 622 349
Size: 273 4970
BglII A'GATC_T
Cuts at: 2029 2758 4795 2029
Size: 729 2037 2477
Fragments arranged by size:
2477 2037 729
BlpI GC'TnA_GC
Cuts at: 1852 2355 1852
Size: 503 4740
Bme1580I G_kGCm'C
Cuts at: 1898 2868 2961 4017 1898
Size: 970 93 1056 3124
Fragments arranged by size:
3124 1056 970 93
BmrI ACTGGGnnnn_n'
Cuts at: 2139 2455 2536 2871 5015 2139
Size: 316 81 335 2144 2367
Fragments arranged by size:
2367 2144 335 316 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3610 3610
Size: 5243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1267 1114
Size: 153 5090
Bpu10I CC'TnA_GC
Cuts at: 871 871
Size: 5243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1128 2419 3298 3790 4088 1128
Size: 1291 879 492 298 2283
Fragments arranged by size:
2283 1291 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4947 4947
Size: 5243
BsaAI yAC'GTr
Cuts at: 2596 2596
Size: 5243
BsaBI GATnn'nnATC
Cuts at: 6 2757 4794 6
Size: 2751 2037 455
Fragments arranged by size:
2751 2037 455
BsaHI Gr'CG_yC
Cuts at: 2674 2922 3624 5120 2674
Size: 248 702 1496 2797
Fragments arranged by size:
2797 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2432 2953 3631 3905 4052 4463 2432
Size: 521 678 274 147 411 3212
Fragments arranged by size:
3212 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2668 3426 4003 4499 2668
Size: 758 577 496 3412
Fragments arranged by size:
3412 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 880
Size: 5243
BsiEI CG_ry'CG
Cuts at: 104 2785 2831 4039 104
Size: 2681 46 1208 1308
Fragments arranged by size:
2681 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1563 1898 2257 3035 3225 4017 1563
Size: 335 359 778 190 792 2789
Fragments arranged by size:
2789 792 778 359 335 190
BsmI GAATG_Cn'
Cuts at: 314 349 1207 1719 2100 314
Size: 35 858 512 381 3457
Fragments arranged by size:
3457 858 512 381 35
Bsp1286I G_dGCh'C
Cuts at: 175 1563 1898 2257 2868 2961 3035 3225 3287
Size: 1388 335 359 611 93 74 190 62
Cuts at: 3287 4017 175
Size: 730 1401
Fragments arranged by size:
1401 1388 730 611 359 335 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4463 4463
Size: 5243
BspHI T'CATG_A
Cuts at: 516 4419 516
Size: 3903 1340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2293 2507 2809 3190 2293
Size: 214 302 381 4346
Fragments arranged by size:
4346 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3535 3589 5073 5238 50
Size: 3485 54 1484 165 55
Fragments arranged by size:
3485 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 674 950 2105 3155 674
Size: 276 1155 1050 2762
Fragments arranged by size:
2762 1155 1050 276
BsrFI r'CCGG_y
Cuts at: 3241 3422 3631 3241
Size: 181 209 4853
Fragments arranged by size:
4853 209 181
BssSI C'ACGA_G
Cuts at: 3514 3872 3514
Size: 358 4885
BstAPI GCAn_nnn'nTGC
Cuts at: 867 1075 2620 867
Size: 208 1545 3490
Fragments arranged by size:
3490 1545 208
BstBI TT'CG_AA
Cuts at: 3604 3604
Size: 5243
BstEII G'GTnAC_C
Cuts at: 876 876
Size: 5243
BstYI r'GATC_y
Cuts at: 1 1299 1446 2029 2268 2735 2758 3093 3339
Size: 1298 147 583 239 467 23 335 246
Cuts at: 3339 4351 4437 4449 4795 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1298 1012 583 467 449 346 335 246
239 147 86 23 12
BtgI C'CryG_G
Cuts at: 1230 1804 1230
Size: 574 4669
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3193 3374 3537 3193
Size: 181 163 4899
Fragments arranged by size:
4899 181 163
BtsI GCAGTG_nn'
Cuts at: 2486 2486
Size: 5243
DraI TTT'AAA
Cuts at: 290 360 2242 290
Size: 70 1882 3291
Fragments arranged by size:
3291 1882 70
DrdI GACnn_nn'nnGTC
Cuts at: 2949 3807 2949
Size: 858 4385
EaeI y'GGCC_r
Cuts at: 2828 3002 3393 3420 4765 2828
Size: 174 391 27 1345 3306
Fragments arranged by size:
3306 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2828 2828
Size: 5243
EarI CTCTTCn'nnn_
Cuts at: 1309 1426 1503 2207 3266 3476 1309
Size: 117 77 704 1059 210 3076
Fragments arranged by size:
3076 1059 704 210 117 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3451 3761 3907 5053 3451
Size: 310 146 1146 3641
Fragments arranged by size:
3641 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2255 2255
Size: 5243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1267 3068 3500 4247 4515 1114
Size: 153 1801 432 747 268 1842
Fragments arranged by size:
1842 1801 747 432 268 153
EcoO109I rG'GnC_Cy
Cuts at: 2382 2382
Size: 5243
EcoRI G'AATT_C
Cuts at: 2247 2247
Size: 5243
EcoRV GAT'ATC
Cuts at: 324 334 324
Size: 10 5233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 526 556 3262 526
Size: 30 2706 2507
Fragments arranged by size:
2706 2507 30
FspI TGC'GCA
Cuts at: 3024 3024
Size: 5243
HaeII r_GCGC'y
Cuts at: 637 711 2677 2925 3947 4926 637
Size: 74 1966 248 1022 979 954
Fragments arranged by size:
1966 1022 979 954 248 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1438 1470 1557 1589 1801 1833 3085 3115 3117
Size: 32 87 32 212 32 1252 30 2
Cuts at: 3117 3147 1438
Size: 30 3534
Fragments arranged by size:
3534 1252 212 87 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 113 929 2282 113
Size: 816 1353 3074
Fragments arranged by size:
3074 1353 816
HindIII A'AGCT_T
Cuts at: 829 829
Size: 5243
KasI G'GCGC_C
Cuts at: 2673 2921 2673
Size: 248 4995
KpnI G_GTAC'C
Cuts at: 2263 2263
Size: 5243
MfeI C'AATT_G
Cuts at: 677 677
Size: 5243
MluI A'CGCG_T
Cuts at: 4676 4676
Size: 5243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3914 4098 3914
Size: 184 5059
MscI TGG'CCA
Cuts at: 3004 3004
Size: 5243
MslI CAynn'nnrTG
Cuts at: 667 1829 2093 3232 3359 667
Size: 1162 264 1139 127 2551
Fragments arranged by size:
2551 1162 1139 264 127
MspA1I CmG'CkG
Cuts at: 1800 1806 2354 2668 3028 4041 4286 1800
Size: 6 548 314 360 1013 245 2757
Fragments arranged by size:
2757 1013 548 360 314 245 6
NaeI GCC'GGC
Cuts at: 3424 3424
Size: 5243
NarI GG'CG_CC
Cuts at: 2674 2922 2674
Size: 248 4995
NcoI C'CATG_G
Cuts at: 1230 1230
Size: 5243
NdeI CA'TA_TG
Cuts at: 764 764
Size: 5243
NgoMIV G'CCGG_C
Cuts at: 3422 3422
Size: 5243
NsiI A_TGCA'T
Cuts at: 351 624 351
Size: 273 4970
NspI r_CATG'y
Cuts at: 1152 2296 2796 3703 1152
Size: 1144 500 907 2692
Fragments arranged by size:
2692 1144 907 500
PciI A'CATG_T
Cuts at: 1148 3699 1148
Size: 2551 2692
PflMI CCAn_nnn'nTGG
Cuts at: 1296 1296
Size: 5243
PfoI T'CCnGG_A
Cuts at: 1193 1193
Size: 5243
PmeI GTTT'AAAC
Cuts at: 2242 2242
Size: 5243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 5211
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 790 822 790
Size: 32 5211
PstI C_TGCA'G
Cuts at: 2290 2975 2290
Size: 685 4558
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5243
PvuII CAG'CTG
Cuts at: 1800 2668 3028 1800
Size: 868 360 4015
Fragments arranged by size:
4015 868 360
RsrII CG'GwC_CG
Cuts at: 3438 3438
Size: 5243
SacI G_AGCT'C
Cuts at: 2257 2257
Size: 5243
SacII CC_GC'GG
Cuts at: 1807 1807
Size: 5243
SalI G'TCGA_C
Cuts at: 2280 2280
Size: 5243
SapI GCTCTTCn'nnn_
Cuts at: 2207 3266 3476 2207
Size: 1059 210 3974
Fragments arranged by size:
3974 1059 210
SbfI CC_TGCA'GG
Cuts at: 2290 2290
Size: 5243
ScaI AGT'ACT
Cuts at: 1160 4760 1160
Size: 3600 1643
SfcI C'TryA_G
Cuts at: 33 1991 2286 2971 3964 4155 33
Size: 1958 295 685 993 191 1121
Fragments arranged by size:
1958 1121 993 685 295 191
SfoI GGC'GCC
Cuts at: 2675 2923 2675
Size: 248 4995
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5243
SmaI CCC'GGG
Cuts at: 2265 2265
Size: 5243
SmlI C'TyrA_G
Cuts at: 1081 1107 2398 3313 3805 4067 1081
Size: 26 1291 915 492 262 2257
Fragments arranged by size:
2257 1291 915 492 262 26
SpeI A'CTAG_T
Cuts at: 67 993 67
Size: 926 4317
SphI G_CATG'C
Cuts at: 2296 2796 2296
Size: 500 4743
StyI C'CwwG_G
Cuts at: 545 1230 1392 2377 2731 545
Size: 685 162 985 354 3057
Fragments arranged by size:
3057 985 685 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1223 2826 2959 3418 3627 1223
Size: 1603 133 459 209 2839
Fragments arranged by size:
2839 1603 459 209 133
TatI w'GTAC_w
Cuts at: 202 1158 4758 202
Size: 956 3600 687
Fragments arranged by size:
3600 956 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4760 4792 4760
Size: 32 5211
Tth111I GACn'n_nGTC
Cuts at: 3040 3040
Size: 5243
XbaI T'CTAG_A
Cuts at: 2274 2274
Size: 5243
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2738 2738
Size: 5243
XmaI C'CCGG_G
Cuts at: 2263 2263
Size: 5243
XmnI GAAnn'nnTTC
Cuts at: 138 536 2201 138
Size: 398 1665 3180
Fragments arranged by size:
3180 1665 398
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsaXI BseRI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI NheI NotI NruI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI