Restriction map for
pF1KE1075
(Circular) (Six-base) MAPSORT of: pF1KE1075.seq Check: 9440 from: 1 to: 5474
pF1KE1075 5474 bp
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AccI GT'mk_AC
Cuts at: 2512 2512
Size: 5474
Acc65I G'GTAC_C
Cuts at: 2490 2490
Size: 5474
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 3299 3731 4478 4746 2243
Size: 1056 432 747 268 2971
Fragments arranged by size:
2971 1056 747 432 268
AflIII A'CryG_T
Cuts at: 958 2826 3930 4907 958
Size: 1868 1104 977 1525
Fragments arranged by size:
1868 1525 1104 977
AgeI A'CCGG_T
Cuts at: 3862 3862
Size: 5474
AhdI GACnn_n'nnGTC
Cuts at: 120 120
Size: 5474
AleI CACnn'nnGTG
Cuts at: 1096 1096
Size: 5474
AlwNI CAG_nnn'CTG
Cuts at: 1132 2779 4346 1132
Size: 1647 1567 2260
Fragments arranged by size:
2260 1647 1567
ApaLI G'TGCA_C
Cuts at: 1113 4244 1113
Size: 3131 2343
ApoI r'AATT_y
Cuts at: 761 1254 1332 1445 1711 1803 1897 2087 2125
Size: 493 78 113 266 92 94 190 38
Cuts at: 2125 2316 2478 4827 5083 761
Size: 191 162 2349 256 1152
Fragments arranged by size:
2349 1152 493 266 256 191 190 162
113 94 92 78 38
AseI AT'TA_AT
Cuts at: 20 1418 1667 20
Size: 1398 249 3827
Fragments arranged by size:
3827 1398 249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 117 2494 117
Size: 2377 3097
AvrII C'CTAG_G
Cuts at: 1912 1912
Size: 5474
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2393 2426 2393
Size: 33 5441
BamHI G'GATC_C
Cuts at: 2499 2499
Size: 5474
BanI G'GyrC_C
Cuts at: 2490 2904 3152 3187 2490
Size: 414 248 35 4777
Fragments arranged by size:
4777 414 248 35
BanII G_rGCy'C
Cuts at: 2488 3518 2488
Size: 1030 4444
BbeI G_GCGC'C
Cuts at: 2908 3156 2908
Size: 248 5226
BbsI GAAGACnn'nnnn_
Cuts at: 197 2424 197
Size: 2227 3247
BciVI GTATCCnnnnn_n'
Cuts at: 3372 4133 5474 3372
Size: 761 1341 3372
Fragments arranged by size:
3372 1341 761
BclI T'GATC_A
Cuts at: 1775 1850 2994 1775
Size: 75 1144 4255
Fragments arranged by size:
4255 1144 75
BfrBI ATG'CAT
Cuts at: 1600 1620 1600
Size: 20 5454
BglII A'GATC_T
Cuts at: 2989 5026 2989
Size: 2037 3437
BlpI GC'TnA_GC
Cuts at: 2586 2586
Size: 5474
Bme1580I G_kGCm'C
Cuts at: 1117 3099 3192 4248 1117
Size: 1982 93 1056 2343
Fragments arranged by size:
2343 1982 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2332 2686 2767 3102 5246 2332
Size: 354 81 335 2144 2560
Fragments arranged by size:
2560 2144 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3841 3841
Size: 5474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 1093 1328 131
Size: 962 235 4277
Fragments arranged by size:
4277 962 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2650 3529 4021 4319 2650
Size: 879 492 298 3805
Fragments arranged by size:
3805 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 107 219 5178 107
Size: 112 4959 403
Fragments arranged by size:
4959 403 112
BsaAI yAC'GTr
Cuts at: 2827 2827
Size: 5474
BsaBI GATnn'nnATC
Cuts at: 6 2988 5025 6
Size: 2982 2037 455
Fragments arranged by size:
2982 2037 455
BsaHI Gr'CG_yC
Cuts at: 2905 3153 3855 5351 2905
Size: 248 702 1496 3028
Fragments arranged by size:
3028 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2663 3184 3862 4136 4283 4694 2663
Size: 521 678 274 147 411 3443
Fragments arranged by size:
3443 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2899 3657 4234 4730 2899
Size: 758 577 496 3643
Fragments arranged by size:
3643 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 5474
BsiEI CG_ry'CG
Cuts at: 104 3016 3062 4270 104
Size: 2912 46 1208 1308
Fragments arranged by size:
2912 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1117 2488 3266 3456 4248 1117
Size: 1371 778 190 792 2343
Fragments arranged by size:
2343 1371 792 778 190
BsmI GAATG_Cn'
Cuts at: 1044 1044
Size: 5474
Bsp1286I G_dGCh'C
Cuts at: 1117 2488 3099 3192 3266 3456 3518 4248 1117
Size: 1371 611 93 74 190 62 730 2343
Fragments arranged by size:
2343 1371 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4694 4694
Size: 5474
BspHI T'CATG_A
Cuts at: 4650 4650
Size: 5474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2524 2738 3040 3421 2524
Size: 214 302 381 4577
Fragments arranged by size:
4577 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3766 3820 5304 5469 50
Size: 3716 54 1484 165 55
Fragments arranged by size:
3716 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2204 3386 2204
Size: 1182 4292
BsrFI r'CCGG_y
Cuts at: 3472 3653 3862 3472
Size: 181 209 5084
Fragments arranged by size:
5084 209 181
BsrGI T'GTAC_A
Cuts at: 2260 2260
Size: 5474
BssSI C'ACGA_G
Cuts at: 3745 4103 3745
Size: 358 5116
BstAPI GCAn_nnn'nTGC
Cuts at: 2851 2851
Size: 5474
BstBI TT'CG_AA
Cuts at: 3835 3835
Size: 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 515 515
Size: 5474
BstYI r'GATC_y
Cuts at: 1 2499 2966 2989 3324 3570 4582 4668 4680
Size: 2498 467 23 335 246 1012 86 12
Cuts at: 4680 5026 1
Size: 346 449
Fragments arranged by size:
2498 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 158 158
Size: 5474
BtgI C'CryG_G
Cuts at: 107 753 2047 107
Size: 646 1294 3534
Fragments arranged by size:
3534 1294 646
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3424 3605 3768 3424
Size: 181 163 5130
Fragments arranged by size:
5130 181 163
BtsI GCAGTG_nn'
Cuts at: 396 2717 396
Size: 2321 3153
ClaI AT'CG_AT
Cuts at: 245 1371 245
Size: 1126 4348
DraI TTT'AAA
Cuts at: 344 953 1160 1217 1364 1427 1808 1869 2093
Size: 609 207 57 147 63 381 61 224
Cuts at: 2093 2473 344
Size: 380 3345
Fragments arranged by size:
3345 609 381 380 224 207 147 63
61 57
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4038 3180
Size: 858 4616
EaeI y'GGCC_r
Cuts at: 3059 3233 3624 3651 4996 3059
Size: 174 391 27 1345 3537
Fragments arranged by size:
3537 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3059 3059
Size: 5474
EarI CTCTTCn'nnn_
Cuts at: 1028 1645 1913 3497 3707 1028
Size: 617 268 1584 210 2795
Fragments arranged by size:
2795 1584 617 268 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3682 3992 4138 5284 3682
Size: 310 146 1146 3872
Fragments arranged by size:
3872 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2486 2486
Size: 5474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 1093 1328 2243 3299 3731 4478 4746 131
Size: 962 235 915 1056 432 747 268 859
Fragments arranged by size:
1056 962 915 859 747 432 268 235
EcoO109I rG'GnC_Cy
Cuts at: 2613 2613
Size: 5474
EcoRI G'AATT_C
Cuts at: 2478 2478
Size: 5474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5474
FspI TGC'GCA
Cuts at: 3255 3255
Size: 5474
HaeII r_GCGC'y
Cuts at: 281 2908 3156 4178 5157 281
Size: 2627 248 1022 979 598
Fragments arranged by size:
2627 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 378 410 1054 1086 1384 1416 2281
Size: 32 240 32 644 32 298 32 865
Cuts at: 2281 2313 3316 3346 3348 3378 106
Size: 32 1003 30 2 30 2202
Fragments arranged by size:
2202 1003 865 644 298 240 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 173 1175 2423 2513 173
Size: 1002 1248 90 3134
Fragments arranged by size:
3134 1248 1002 90
HindIII A'AGCT_T
Cuts at: 707 1486 707
Size: 779 4695
KasI G'GCGC_C
Cuts at: 2904 3152 2904
Size: 248 5226
KpnI G_GTAC'C
Cuts at: 2494 2494
Size: 5474
MfeI C'AATT_G
Cuts at: 1378 2446 1378
Size: 1068 4406
MluI A'CGCG_T
Cuts at: 4907 4907
Size: 5474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 104 496 4145 4329 104
Size: 392 3649 184 1249
Fragments arranged by size:
3649 1249 392 184
MscI TGG'CCA
Cuts at: 3235 3235
Size: 5474
MslI CAynn'nnrTG
Cuts at: 641 1096 3463 3590 641
Size: 455 2367 127 2525
Fragments arranged by size:
2525 2367 455 127
MspA1I CmG'CkG
Cuts at: 151 2585 2899 3259 4272 4517 151
Size: 2434 314 360 1013 245 1108
Fragments arranged by size:
2434 1108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3655 3655
Size: 5474
NarI GG'CG_CC
Cuts at: 2905 3153 2905
Size: 248 5226
NcoI C'CATG_G
Cuts at: 107 753 2047 107
Size: 646 1294 3534
Fragments arranged by size:
3534 1294 646
NgoMIV G'CCGG_C
Cuts at: 3653 3653
Size: 5474
NsiI A_TGCA'T
Cuts at: 1602 1622 1602
Size: 20 5454
NspI r_CATG'y
Cuts at: 640 1568 1600 2527 3027 3934 640
Size: 928 32 927 500 907 2180
Fragments arranged by size:
2180 928 927 907 500 32
PciI A'CATG_T
Cuts at: 3930 3930
Size: 5474
PflMI CCAn_nnn'nTGG
Cuts at: 656 2391 656
Size: 1735 3739
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4613 4645 4613
Size: 32 5442
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 582 614 582
Size: 32 5442
PstI C_TGCA'G
Cuts at: 403 2521 3206 403
Size: 2118 685 2671
Fragments arranged by size:
2671 2118 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 151 2899 3259 151
Size: 2748 360 2366
Fragments arranged by size:
2748 2366 360
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 2488 2488
Size: 5474
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 1913 3497 3707 1913
Size: 1584 210 3680
Fragments arranged by size:
3680 1584 210
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 1721 4991 1721
Size: 3270 2204
SfcI C'TryA_G
Cuts at: 33 399 1073 1178 1499 2396 2517 3202 4195
Size: 366 674 105 321 897 121 685 993
Cuts at: 4195 4386 33
Size: 191 1121
Fragments arranged by size:
1121 993 897 685 674 366 321 191
121 105
SfoI GGC'GCC
Cuts at: 2906 3154 2906
Size: 248 5226
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 119 2496 119
Size: 2377 3097
SmlI C'TyrA_G
Cuts at: 2629 3544 4036 4298 2629
Size: 915 492 262 3805
Fragments arranged by size:
3805 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5474
SphI G_CATG'C
Cuts at: 1600 2527 3027 1600
Size: 927 500 4047
Fragments arranged by size:
4047 927 500
SspI AAT'ATT
Cuts at: 834 1026 1243 1433 1755 834
Size: 192 217 190 322 4553
Fragments arranged by size:
4553 322 217 192 190
StyI C'CwwG_G
Cuts at: 107 650 753 1912 2047 2608 2962 107
Size: 543 103 1159 135 561 354 2619
Fragments arranged by size:
2619 1159 561 543 354 135 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3057 3190 3649 3858 3057
Size: 133 459 209 4673
Fragments arranged by size:
4673 459 209 133
TatI w'GTAC_w
Cuts at: 1719 2260 4989 1719
Size: 541 2729 2204
Fragments arranged by size:
2729 2204 541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 4991 5023 2043
Size: 32 2916 32 2494
Fragments arranged by size:
2916 2494 32 32
Tth111I GACn'n_nGTC
Cuts at: 3271 3271
Size: 5474
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1794 2969 1794
Size: 1175 4299
XmaI C'CCGG_G
Cuts at: 117 2494 117
Size: 2377 3097
XmnI GAAnn'nnTTC
Cuts at: 1334 1334
Size: 5474
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BsmBI BssHII BstEII BstZ17I CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI