Restriction map for
pF1KE1082
(Circular) (Six-base) MAPSORT of: pF1KE1082.seq Check: 5840 from: 1 to: 4430
pF1KE1082 4430 bp
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4430
AclI AA'CG_TT
Cuts at: 119 4270 119
Size: 4151 279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1373 2255 2687 3434 3702 1373
Size: 882 432 747 268 2101
Fragments arranged by size:
2101 882 747 432 268
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 617 648 943 1735 3302 617
Size: 31 295 792 1567 1745
Fragments arranged by size:
1745 1567 792 295 31
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 262 1434 3783 4039 262
Size: 1172 2349 256 653
Fragments arranged by size:
2349 1172 653 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4430
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 811 1450 811
Size: 639 3791
BamHI G'GATC_C
Cuts at: 1455 1455
Size: 4430
BanI G'GyrC_C
Cuts at: 237 1004 1446 1860 2108 2143 237
Size: 767 442 414 248 35 2524
Fragments arranged by size:
2524 767 442 414 248 35
BanII G_rGCy'C
Cuts at: 314 818 1444 2474 314
Size: 504 626 1030 2270
Fragments arranged by size:
2270 1030 626 504
BbeI G_GCGC'C
Cuts at: 241 1864 2112 241
Size: 1623 248 2559
Fragments arranged by size:
2559 1623 248
BbsI GAAGACnn'nnnn_
Cuts at: 681 681
Size: 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4396
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglII A'GATC_T
Cuts at: 1945 3982 1945
Size: 2037 2393
BlpI GC'TnA_GC
Cuts at: 1542 1542
Size: 4430
Bme1580I G_kGCm'C
Cuts at: 2055 2148 3204 2055
Size: 93 1056 3281
Fragments arranged by size:
3281 1056 93
BmgBI CAC'GTC
Cuts at: 1011 1011
Size: 4430
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 938 2797 938
Size: 1859 2571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 612 729 980 424
Size: 188 117 251 3874
Fragments arranged by size:
3874 251 188 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1606 2485 2977 3275 982
Size: 624 879 492 298 2137
Fragments arranged by size:
2137 879 624 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 466 4134 466
Size: 3668 762
BsaAI yAC'GTr
Cuts at: 1783 1783
Size: 4430
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 238 1861 2109 2811 4307 238
Size: 1623 248 702 1496 361
Fragments arranged by size:
1623 1496 702 361 248
BsaWI w'CCGG_w
Cuts at: 1619 2140 2818 3092 3239 3650 1619
Size: 521 678 274 147 411 2399
Fragments arranged by size:
2399 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 422 452 422
Size: 30 4400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 205 443 771 847 880 968 1174 1218
Size: 3 238 328 76 33 88 206 44
Cuts at: 1218 1348 202
Size: 130 3284
Fragments arranged by size:
3284 328 238 206 130 88 76 44
33 3
BseYI C'CCAG_C
Cuts at: 241 423 1855 2613 3190 3686 241
Size: 182 1432 758 577 496 985
Fragments arranged by size:
1432 985 758 577 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4430
BsiEI CG_ry'CG
Cuts at: 104 395 527 1972 2018 3226 104
Size: 291 132 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 291 132 46
BsiHKAI G_wGCw'C
Cuts at: 827 1444 2222 2412 3204 827
Size: 617 778 190 792 2053
Fragments arranged by size:
2053 792 778 617 190
BsmBI CGTCTCn'nnnn_
Cuts at: 546 1017 546
Size: 471 3959
Bsp1286I G_dGCh'C
Cuts at: 314 818 827 1444 2055 2148 2222 2412 2474
Size: 504 9 617 611 93 74 190 62
Cuts at: 2474 3204 314
Size: 730 1540
Fragments arranged by size:
1540 730 617 611 504 190 93 74
62 9
BspEI T'CCGG_A
Cuts at: 3650 3650
Size: 4430
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 647 1480 1694 1996 2377 647
Size: 833 214 302 381 2700
Fragments arranged by size:
2700 833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 305 515 2722 2776 4260 4425 50
Size: 255 210 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 255 210 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 2428 2609 2818 2428
Size: 181 209 4040
Fragments arranged by size:
4040 209 181
BssHII G'CGCG_C
Cuts at: 571 571
Size: 4430
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstEII G'GTnAC_C
Cuts at: 411 411
Size: 4430
BstYI r'GATC_y
Cuts at: 1 645 1247 1455 1922 1945 2280 2526 3538
Size: 644 602 208 467 23 335 246 1012
Cuts at: 3538 3624 3636 3982 1
Size: 86 12 346 449
Fragments arranged by size:
1012 644 602 467 449 346 335 246
208 86 23 12
BtgI C'CryG_G
Cuts at: 389 603 389
Size: 214 4216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4086
Fragments arranged by size:
4086 181 163
BtsI GCAGTG_nn'
Cuts at: 1673 1673
Size: 4430
ClaI AT'CG_AT
Cuts at: 893 893
Size: 4430
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2994 2136
Size: 858 3572
EaeI y'GGCC_r
Cuts at: 392 524 606 2015 2189 2580 2607 3952 392
Size: 132 82 1409 174 391 27 1345 870
Fragments arranged by size:
1409 1345 870 391 174 132 82 27
EagI C'GGCC_G
Cuts at: 392 524 2015 392
Size: 132 1491 2807
Fragments arranged by size:
2807 1491 132
EarI CTCTTCn'nnn_
Cuts at: 1349 2453 2663 1349
Size: 1104 210 3116
Fragments arranged by size:
3116 1104 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2948 3094 4240 2638
Size: 310 146 1146 2828
Fragments arranged by size:
2828 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 612 729 980 1373 2255 2687 3434 3702
Size: 188 117 251 393 882 432 747 268
Cuts at: 3702 424
Size: 1152
Fragments arranged by size:
1152 882 747 432 393 268 251 188
117
EcoO109I rG'GnC_Cy
Cuts at: 1145 1275 1569 1145
Size: 130 294 4006
Fragments arranged by size:
4006 294 130
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
EcoRV GAT'ATC
Cuts at: 252 252
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4430
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 241 656 752 1042 1200 1864 2112 3134 4113
Size: 415 96 290 158 664 248 1022 979
Cuts at: 4113 241
Size: 558
Fragments arranged by size:
1022 979 664 558 415 290 248 158
96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 700 732 931 963 2272 2302 2304
Size: 32 526 32 199 32 1309 30 2
Cuts at: 2304 2334 142
Size: 30 2238
Fragments arranged by size:
2238 1309 526 199 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 237 1860 2108 237
Size: 1623 248 2559
Fragments arranged by size:
2559 1623 248
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4430
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1238 3101 3285 1067
Size: 171 1863 184 2212
Fragments arranged by size:
2212 1863 184 171
MscI TGG'CCA
Cuts at: 2191 2191
Size: 4430
MslI CAynn'nnrTG
Cuts at: 2419 2546 2419
Size: 127 4303
MspA1I CmG'CkG
Cuts at: 402 584 605 1541 1855 2215 3228 3473 402
Size: 182 21 936 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 936 360 314 245 182 21
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 238 1861 2109 238
Size: 1623 248 2559
Fragments arranged by size:
2559 1623 248
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NspI r_CATG'y
Cuts at: 1483 1983 2890 1483
Size: 500 907 3023
Fragments arranged by size:
3023 907 500
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PfoI T'CCnGG_A
Cuts at: 497 1250 497
Size: 753 3677
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4398
PpuMI rG'GwC_Cy
Cuts at: 1145 1145
Size: 4430
PstI C_TGCA'G
Cuts at: 230 644 1477 2162 230
Size: 414 833 685 2498
Fragments arranged by size:
2498 833 685 414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 402 1855 2215 402
Size: 1453 360 2617
Fragments arranged by size:
2617 1453 360
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SacII CC_GC'GG
Cuts at: 606 606
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4220
SbfI CC_TGCA'GG
Cuts at: 644 1477 644
Size: 833 3597
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SfcI C'TryA_G
Cuts at: 33 226 640 1473 2158 3151 3342 33
Size: 193 414 833 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 414 193 191
SfoI GGC'GCC
Cuts at: 239 1862 2110 239
Size: 1623 248 2559
Fragments arranged by size:
2559 1623 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4430
SmlI C'TyrA_G
Cuts at: 997 1585 2500 2992 3254 997
Size: 588 915 492 262 2173
Fragments arranged by size:
2173 915 588 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
StyI C'CwwG_G
Cuts at: 1280 1564 1918 1280
Size: 284 354 3792
Fragments arranged by size:
3792 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 430 2013 2146 2605 2814 430
Size: 1583 133 459 209 2046
Fragments arranged by size:
2046 1583 459 209 133
TatI w'GTAC_w
Cuts at: 3945 3945
Size: 4430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 493 525 1404 1436 3947 3979 422
Size: 32 39 32 879 32 2511 32 873
Fragments arranged by size:
2511 879 873 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 789 1093 2227 789
Size: 304 1134 2992
Fragments arranged by size:
2992 1134 304
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1925 1925
Size: 4430
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4430
XmnI GAAnn'nnTTC
Cuts at: 262 262
Size: 4430
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI