Restriction map for
pF1KE1083
(Circular) (Six-base) MAPSORT of: pF1KE1083.seq Check: 8712 from: 1 to: 4223
pF1KE1083 4223 bp
With 182 enzymes: SgfI *
July 22, 2009 17:02 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1138 2048 2480 3227 3495 923
Size: 215 910 432 747 268 1651
Fragments arranged by size:
1651 910 747 432 268 215
AflIII A'CryG_T
Cuts at: 1575 2679 3656 1575
Size: 1104 977 2142
Fragments arranged by size:
2142 1104 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 1528 3095 1528
Size: 1567 2656
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 507 1227 3576 3832 507
Size: 720 2349 256 898
Fragments arranged by size:
2349 898 720 256
AseI AT'TA_AT
Cuts at: 20 532 20
Size: 512 3711
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 1243 1243
Size: 4223
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 1239 1653 1901 1936 1239
Size: 414 248 35 3526
Fragments arranged by size:
3526 414 248 35
BanII G_rGCy'C
Cuts at: 804 1237 2267 804
Size: 433 1030 2760
Fragments arranged by size:
2760 1030 433
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BbsI GAAGACnn'nnnn_
Cuts at: 1151 1151
Size: 4223
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BfrBI ATG'CAT
Cuts at: 666 666
Size: 4223
BglII A'GATC_T
Cuts at: 849 1738 3775 849
Size: 889 2037 1297
Fragments arranged by size:
2037 1297 889
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 1848 1941 2997 1848
Size: 93 1056 3074
Fragments arranged by size:
3074 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1435 1516 1851 3995 1435
Size: 81 335 2144 1663
Fragments arranged by size:
2144 1663 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
Bpu10I CC'TnA_GC
Cuts at: 412 762 412
Size: 350 3873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 887 1399 2278 2770 3068 887
Size: 512 879 492 298 2042
Fragments arranged by size:
2042 879 512 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 545 1737 3774 6
Size: 539 1192 2037 455
Fragments arranged by size:
2037 1192 539 455
BsaHI Gr'CG_yC
Cuts at: 1654 1902 2604 4100 1654
Size: 248 702 1496 1777
Fragments arranged by size:
1777 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 250 275 799 247
Size: 3 25 524 3671
Fragments arranged by size:
3671 524 25 3
BseYI C'CCAG_C
Cuts at: 1648 2406 2983 3479 1648
Size: 758 577 496 2392
Fragments arranged by size:
2392 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 153 1110 1237 2015 2205 2997 153
Size: 957 127 778 190 792 1379
Fragments arranged by size:
1379 957 792 778 190 127
BsmBI CGTCTCn'nnnn_
Cuts at: 951 951
Size: 4223
Bsp1286I G_dGCh'C
Cuts at: 153 804 1110 1237 1848 1941 2015 2205 2267
Size: 651 306 127 611 93 74 190 62
Cuts at: 2267 2997 153
Size: 730 1379
Fragments arranged by size:
1379 730 651 611 306 190 127 93
74 62
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 1487 1789 2170 1273
Size: 214 302 381 3326
Fragments arranged by size:
3326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 156 357 2135 156
Size: 201 1778 2244
Fragments arranged by size:
2244 1778 201
BsrFI r'CCGG_y
Cuts at: 2221 2402 2611 2221
Size: 181 209 3833
Fragments arranged by size:
3833 209 181
BssSI C'ACGA_G
Cuts at: 124 2494 2852 124
Size: 2370 358 1495
Fragments arranged by size:
2370 1495 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1600 1600
Size: 4223
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstYI r'GATC_y
Cuts at: 1 849 1248 1715 1738 2073 2319 3331 3417
Size: 848 399 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 848 467 449 399 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 197 107
Size: 90 4133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1466
Size: 4223
ClaI AT'CG_AT
Cuts at: 419 419
Size: 4223
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DraIII CAC_nnn'GTG
Cuts at: 148 148
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 1808 1982 2373 2400 3745 1808
Size: 174 391 27 1345 2286
Fragments arranged by size:
2286 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 938 949 2246 2456 938
Size: 11 1297 210 2705
Fragments arranged by size:
2705 1297 210 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1138 2048 2480 3227 3495 923
Size: 215 910 432 747 268 1651
Fragments arranged by size:
1651 910 747 432 268 215
EcoO109I rG'GnC_Cy
Cuts at: 1053 1362 1053
Size: 309 3914
EcoRI G'AATT_C
Cuts at: 507 1227 507
Size: 720 3503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 160 1168 2242 160
Size: 1008 1074 2141
Fragments arranged by size:
2141 1074 1008
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1657 1905 2927 3906 1657
Size: 248 1022 979 1974
Fragments arranged by size:
1974 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1059 1091 2065 2095 2097 2127 1059
Size: 32 974 30 2 30 3155
Fragments arranged by size:
3155 974 32 30 30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MfeI C'AATT_G
Cuts at: 212 212
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 681 2894 3078 681
Size: 2213 184 1826
Fragments arranged by size:
2213 1826 184
MscI TGG'CCA
Cuts at: 1984 1984
Size: 4223
MslI CAynn'nnrTG
Cuts at: 444 1077 1079 2212 2339 444
Size: 633 2 1133 127 2328
Fragments arranged by size:
2328 1133 633 127 2
MspA1I CmG'CkG
Cuts at: 225 1162 1334 1648 2008 3021 3266 225
Size: 937 172 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 937 360 314 245 172
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NcoI C'CATG_G
Cuts at: 107 107
Size: 4223
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NsiI A_TGCA'T
Cuts at: 668 668
Size: 4223
NspI r_CATG'y
Cuts at: 1152 1276 1776 2683 1152
Size: 124 500 907 2692
Fragments arranged by size:
2692 907 500 124
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PpuMI rG'GwC_Cy
Cuts at: 1053 1053
Size: 4223
PsiI TTA'TAA
Cuts at: 346 455 346
Size: 109 4114
PstI C_TGCA'G
Cuts at: 644 1270 1955 644
Size: 626 685 2912
Fragments arranged by size:
2912 685 626
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 225 1162 1648 2008 225
Size: 937 486 360 2440
Fragments arranged by size:
2440 937 486 360
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 938 2246 2456 938
Size: 1308 210 2705
Fragments arranged by size:
2705 1308 210
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 640 1266 1951 2944 3135 33
Size: 607 626 685 993 191 1121
Fragments arranged by size:
1121 993 685 626 607 191
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 902 1378 2293 2785 3047 902
Size: 476 915 492 262 2078
Fragments arranged by size:
2078 915 492 476 262
SpeI A'CTAG_T
Cuts at: 67 268 67
Size: 201 4022
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
SspI AAT'ATT
Cuts at: 187 187
Size: 4223
StyI C'CwwG_G
Cuts at: 107 1357 1711 107
Size: 1250 354 2619
Fragments arranged by size:
2619 1250 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1806 1939 2398 2607 1806
Size: 133 459 209 3422
Fragments arranged by size:
3422 459 209 133
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4191
Tth111I GACn'n_nGTC
Cuts at: 1170 2020 1170
Size: 850 3373
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
XmnI GAAnn'nnTTC
Cuts at: 947 947
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI