Restriction map for
pF1KE1084
(Circular) (Six-base) MAPSORT of: pF1KE1084.seq Check: 2638 from: 1 to: 3749
pF1KE1084 3749 bp
With 182 enzymes: SgfI *
June 30, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 479 787 479
Size: 308 3441
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1574 2006 2753 3021 239
Size: 1335 432 747 268 967
Fragments arranged by size:
1335 967 747 432 268
AflIII A'CryG_T
Cuts at: 319 335 1101 2205 3182 319
Size: 16 766 1104 977 886
Fragments arranged by size:
1104 977 886 766 16
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 3717
AlwNI CAG_nnn'CTG
Cuts at: 567 663 1054 2621 567
Size: 96 391 1567 1695
Fragments arranged by size:
1695 1567 391 96
ApaLI G'TGCA_C
Cuts at: 2519 2519
Size: 3749
ApoI r'AATT_y
Cuts at: 275 753 3102 3358 275
Size: 478 2349 256 666
Fragments arranged by size:
2349 666 478 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 774 774
Size: 3749
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 732 763 1793 732
Size: 31 1030 2688
Fragments arranged by size:
2688 1030 31
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 861 861
Size: 3749
Bme1580I G_kGCm'C
Cuts at: 1374 1467 2523 1374
Size: 93 1056 2600
Fragments arranged by size:
2600 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 187 961 1042 1377 3521 187
Size: 774 81 335 2144 415
Fragments arranged by size:
2144 774 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 1102 1102
Size: 3749
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 938 1459 2137 2411 2558 2969 938
Size: 521 678 274 147 411 1718
Fragments arranged by size:
1718 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 103
Size: 30 3719
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 445
Size: 3749
BseYI C'CCAG_C
Cuts at: 1174 1932 2509 3005 1174
Size: 758 577 496 1918
Fragments arranged by size:
1918 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 3749
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 763 1541 1731 2523 763
Size: 778 190 792 1989
Fragments arranged by size:
1989 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 732 763 1374 1467 1541 1731 1793 2523 732
Size: 31 611 93 74 190 62 730 1958
Fragments arranged by size:
1958 730 611 190 93 74 62 31
BspEI T'CCGG_A
Cuts at: 2969 2969
Size: 3749
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 799 1013 1315 1696 799
Size: 214 302 381 2852
Fragments arranged by size:
2852 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 1747 1928 2137 1747
Size: 181 209 3359
Fragments arranged by size:
3359 209 181
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 2110 2110
Size: 3749
BstYI r'GATC_y
Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955
Size: 773 467 23 335 246 1012 86 12
Cuts at: 2955 3301 1
Size: 346 449
Fragments arranged by size:
1012 773 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 480 480
Size: 3749
Bsu36I CC'TnA_GG
Cuts at: 724 724
Size: 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 992 992
Size: 3749
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 1334 1508 1899 1926 3271 1334
Size: 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 258 737 1772 1982 258
Size: 479 1035 210 2025
Fragments arranged by size:
2025 1035 479 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 478 1574 2006 2753 3021 239
Size: 239 1096 432 747 268 967
Fragments arranged by size:
1096 967 747 432 268 239
EcoNI CCTnn'n_nnAGG
Cuts at: 310 310
Size: 3749
EcoO109I rG'GnC_Cy
Cuts at: 888 888
Size: 3749
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 694 1530 694
Size: 836 2913
HaeII r_GCGC'y
Cuts at: 131 1183 1431 2453 3432 131
Size: 1052 248 1022 979 448
Fragments arranged by size:
1052 1022 979 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 283 315 666 698 1591 1621 1623
Size: 32 21 32 351 32 893 30 2
Cuts at: 1623 1653 230
Size: 30 2326
Fragments arranged by size:
2326 893 351 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 788 788
Size: 3749
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 1510 1510
Size: 3749
MslI CAynn'nnrTG
Cuts at: 1738 1865 1738
Size: 127 3622
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 323 339 802 1302 2209 323
Size: 16 463 500 907 1863
Fragments arranged by size:
1863 907 500 463 16
PasI CC'CwG_GG
Cuts at: 153 153
Size: 3749
PciI A'CATG_T
Cuts at: 319 335 2205 319
Size: 16 1870 1863
Fragments arranged by size:
1870 1863 16
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2888 2920 103
Size: 32 2753 32 932
Fragments arranged by size:
2753 932 32 32
PstI C_TGCA'G
Cuts at: 572 647 668 716 796 1481 572
Size: 75 21 48 80 685 2840
Fragments arranged by size:
2840 685 80 75 48 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 737 1772 1982 737
Size: 1035 210 2504
Fragments arranged by size:
2504 1035 210
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 568 643 664 712 792 1477 2470 2661
Size: 535 75 21 48 80 685 993 191
Cuts at: 2661 33
Size: 1121
Fragments arranged by size:
1121 993 685 535 191 80 75 48
21
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StyI C'CwwG_G
Cuts at: 883 1237 883
Size: 354 3395
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 124 1332 1465 1924 2133 124
Size: 1208 133 459 209 1740
Fragments arranged by size:
1740 1208 459 209 133
TatI w'GTAC_w
Cuts at: 544 3264 544
Size: 2720 1029
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 3717
Tth111I GACn'n_nGTC
Cuts at: 1546 1546
Size: 3749
XbaI T'CTAG_A
Cuts at: 588 780 588
Size: 192 3557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1244 1244
Size: 3749
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 3749
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI