Restriction map for
pF1KE1090
(Circular) (Six-base) MAPSORT of: pF1KE1090.seq Check: 9195 from: 1 to: 4898
pF1KE1090 4898 bp
With 182 enzymes: SgfI *
October 13, 2009 15:05 ..
AccI GT'mk_AC
Cuts at: 1936 1936
Size: 4898
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 969 2723 3155 3902 4170 969
Size: 1754 432 747 268 1697
Fragments arranged by size:
1754 1697 747 432 268
AflIII A'CryG_T
Cuts at: 959 1668 2250 3354 4331 959
Size: 709 582 1104 977 1526
Fragments arranged by size:
1526 1104 977 709 582
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AleI CACnn'nnGTG
Cuts at: 298 958 298
Size: 660 4238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 477 509 477
Size: 32 4866
AlwNI CAG_nnn'CTG
Cuts at: 1190 1823 2203 3770 1190
Size: 633 380 1567 2318
Fragments arranged by size:
2318 1567 633 380
ApaLI G'TGCA_C
Cuts at: 289 358 3668 289
Size: 69 3310 1519
Fragments arranged by size:
3310 1519 69
ApoI r'AATT_y
Cuts at: 545 1087 1902 4251 4507 545
Size: 542 815 2349 256 936
Fragments arranged by size:
2349 936 815 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 466 1918 466
Size: 1452 3446
BamHI G'GATC_C
Cuts at: 167 1049 1835 1923 167
Size: 882 786 88 3142
Fragments arranged by size:
3142 882 786 88
BanI G'GyrC_C
Cuts at: 187 608 1075 1535 1583 1914 2328 2576 2611
Size: 421 467 460 48 331 414 248 35
Cuts at: 2611 187
Size: 2474
Fragments arranged by size:
2474 467 460 421 414 331 248 48
35
BanII G_rGCy'C
Cuts at: 1703 1912 2942 1703
Size: 209 1030 3659
Fragments arranged by size:
3659 1030 209
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbsI GAAGACnn'nnnn_
Cuts at: 592 1035 592
Size: 443 4455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4864
BciVI GTATCCnnnnn_n'
Cuts at: 482 2796 3557 4898 482
Size: 2314 761 1341 482
Fragments arranged by size:
2314 1341 761 482
BclI T'GATC_A
Cuts at: 212 2418 212
Size: 2206 2692
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4898
BglII A'GATC_T
Cuts at: 872 2413 4450 872
Size: 1541 2037 1320
Fragments arranged by size:
2037 1541 1320
BlpI GC'TnA_GC
Cuts at: 2010 2010
Size: 4898
Bme1580I G_kGCm'C
Cuts at: 293 362 613 2523 2616 3672 293
Size: 69 251 1910 93 1056 1519
Fragments arranged by size:
1910 1519 1056 251 93 69
BmgBI CAC'GTC
Cuts at: 1671 1671
Size: 4898
BmrI ACTGGGnnnn_n'
Cuts at: 888 2110 2191 2526 4670 888
Size: 1222 81 335 2144 1116
Fragments arranged by size:
2144 1222 1116 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1167 1609 1851 1167
Size: 442 242 4214
Fragments arranged by size:
4214 442 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3445 3743 2074
Size: 879 492 298 3229
Fragments arranged by size:
3229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4602 4602
Size: 4898
BsaAI yAC'GTr
Cuts at: 210 2251 210
Size: 2041 2857
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3279 4775 2329
Size: 248 702 1496 2452
Fragments arranged by size:
2452 1496 702 248
BsaWI w'CCGG_w
Cuts at: 553 1078 1838 2087 2608 3286 3560 3707 4118
Size: 525 760 249 521 678 274 147 411
Cuts at: 4118 553
Size: 1333
Fragments arranged by size:
1333 760 678 525 521 411 274 249
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1165 1195 1582 1612 1165
Size: 30 387 30 4451
Fragments arranged by size:
4451 387 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 1492 1606 340
Size: 1152 114 3632
Fragments arranged by size:
3632 1152 114
BseYI C'CCAG_C
Cuts at: 1816 2323 3081 3658 4154 1816
Size: 507 758 577 496 2560
Fragments arranged by size:
2560 758 577 507 496
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 362 680 1912 2690 2880 3672 293
Size: 69 318 1232 778 190 792 1519
Fragments arranged by size:
1519 1232 792 778 318 190 69
BsmBI CGTCTCn'nnnn_
Cuts at: 142 142
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 293 362 613 680 1703 1912 2523 2616 2690
Size: 69 251 67 1023 209 611 93 74
Cuts at: 2690 2880 2942 3672 293
Size: 190 62 730 1519
Fragments arranged by size:
1519 1023 730 611 251 209 190 93
74 69 67 62
BspEI T'CCGG_A
Cuts at: 1838 4118 1838
Size: 2280 2618
BspHI T'CATG_A
Cuts at: 4074 4074
Size: 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1410 1948 2162 2464 2845 1410
Size: 538 214 302 381 3463
Fragments arranged by size:
3463 538 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2810 2810
Size: 4898
BsrFI r'CCGG_y
Cuts at: 111 1736 2896 3077 3286 111
Size: 1625 1160 181 209 1723
Fragments arranged by size:
1723 1625 1160 209 181
BsrGI T'GTAC_A
Cuts at: 1112 1112
Size: 4898
BssSI C'ACGA_G
Cuts at: 720 3169 3527 720
Size: 2449 358 2091
Fragments arranged by size:
2449 2091 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2275 2275
Size: 4898
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstYI r'GATC_y
Cuts at: 1 167 872 1049 1513 1835 1923 2390 2413
Size: 166 705 177 464 322 88 467 23
Cuts at: 2413 2748 2994 4006 4092 4104 4450 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 705 467 464 449 346 335 322
246 177 166 88 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1420 2848 3029 3192 1420
Size: 1428 181 163 3126
Fragments arranged by size:
3126 1428 181 163
BtsI GCAGTG_nn'
Cuts at: 135 629 909 2141 135
Size: 494 280 1232 2892
Fragments arranged by size:
2892 1232 494 280
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3462 2604
Size: 858 4040
EaeI y'GGCC_r
Cuts at: 705 1145 2483 2657 3048 3075 4420 705
Size: 440 1338 174 391 27 1345 1183
Fragments arranged by size:
1345 1338 1183 440 391 174 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 623 1849 2921 3131 623
Size: 1226 1072 210 2390
Fragments arranged by size:
2390 1226 1072 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 3106 3416 3562 4708 869
Size: 2237 310 146 1146 1059
Fragments arranged by size:
2237 1146 1059 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 969 1167 1609 1851 2723 3155 3902 4170 969
Size: 198 442 242 872 432 747 268 1697
Fragments arranged by size:
1697 872 747 442 432 268 242 198
EcoO109I rG'GnC_Cy
Cuts at: 633 1619 2037 633
Size: 986 418 3494
Fragments arranged by size:
3494 986 418
EcoRI G'AATT_C
Cuts at: 545 1902 545
Size: 1357 3541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 2917 219
Size: 2698 2200
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 1714 1779 2332 2580 3602 4581 1714
Size: 65 553 248 1022 979 2031
Fragments arranged by size:
2031 1022 979 553 248 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 759 791 1165 1197 2740 2770 2772 2802 759
Size: 32 374 32 1543 30 2 30 2855
Fragments arranged by size:
2855 1543 374 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1245 1937 1245
Size: 692 4206
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 819 3569 3753 819
Size: 2750 184 1964
Fragments arranged by size:
2750 1964 184
MscI TGG'CCA
Cuts at: 2659 2659
Size: 4898
MslI CAynn'nnrTG
Cuts at: 298 958 1336 2887 3014 298
Size: 660 378 1551 127 2182
Fragments arranged by size:
2182 1551 660 378 127
MspA1I CmG'CkG
Cuts at: 125 264 456 1104 1150 1488 1820 1829 2009
Size: 139 192 648 46 338 332 9 180
Cuts at: 2009 2323 2683 3696 3941 125
Size: 314 360 1013 245 1082
Fragments arranged by size:
1082 1013 648 360 338 332 314 245
192 180 139 46 9
NaeI GCC'GGC
Cuts at: 113 1738 3079 113
Size: 1625 1341 1932
Fragments arranged by size:
1932 1625 1341
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NgoMIV G'CCGG_C
Cuts at: 111 1736 3077 111
Size: 1625 1341 1932
Fragments arranged by size:
1932 1625 1341
NspI r_CATG'y
Cuts at: 1951 2451 3358 1951
Size: 500 907 3491
Fragments arranged by size:
3491 907 500
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PflMI CCAn_nnn'nTGG
Cuts at: 197 1794 197
Size: 1597 3301
PfoI T'CCnGG_A
Cuts at: 1464 1464
Size: 4898
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PmlI CAC'GTG
Cuts at: 210 210
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4866
PstI C_TGCA'G
Cuts at: 124 130 1679 1945 2630 124
Size: 6 1549 266 685 2392
Fragments arranged by size:
2392 1549 685 266 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 125 1104 1488 1820 2323 2683 125
Size: 979 384 332 503 360 2340
Fragments arranged by size:
2340 979 503 384 360 332
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 2921 3131 2921
Size: 210 4688
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 1000 4415 1000
Size: 3415 1483
SfcI C'TryA_G
Cuts at: 33 120 126 1257 1675 1755 1941 2626 3619
Size: 87 6 1131 418 80 186 685 993
Cuts at: 3619 3810 33
Size: 191 1121
Fragments arranged by size:
1131 1121 993 685 418 191 186 87
80 6
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 2053 2968 3460 3722 2053
Size: 915 492 262 3229
Fragments arranged by size:
3229 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StyI C'CwwG_G
Cuts at: 296 2032 2386 296
Size: 1736 354 2808
Fragments arranged by size:
2808 1736 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 518 1129 2481 2614 3073 3282 518
Size: 611 1352 133 459 209 2134
Fragments arranged by size:
2134 1352 611 459 209 133
TatI w'GTAC_w
Cuts at: 998 1112 1868 4413 998
Size: 114 756 2545 1483
Fragments arranged by size:
2545 1483 756 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1582 1614 4415 4447 1582
Size: 32 2801 32 2033
Fragments arranged by size:
2801 2033 32 32
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 621 1434 2393 621
Size: 813 959 3126
Fragments arranged by size:
3126 959 813
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 409 409
Size: 4898
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI
BsmI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI