Restriction map for
pF1KE1096
(Circular) (Six-base) MAPSORT of: pF1KE1096.seq Check: 7180 from: 1 to: 4211
pF1KE1096 4211 bp
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 262 262
Size: 4211
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2468 3215 3483 2036
Size: 432 747 268 2764
Fragments arranged by size:
2764 747 432 268
AfeI AGC'GCT
Cuts at: 515 529 515
Size: 14 4197
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AleI CACnn'nnGTG
Cuts at: 256 256
Size: 4211
AlwNI CAG_nnn'CTG
Cuts at: 1516 3083 1516
Size: 1567 2644
ApaI G_GGCC'C
Cuts at: 638 638
Size: 4211
ApaLI G'TGCA_C
Cuts at: 2981 2981
Size: 4211
ApoI r'AATT_y
Cuts at: 1215 3564 3820 1215
Size: 2349 256 1606
Fragments arranged by size:
2349 1606 256
AseI AT'TA_AT
Cuts at: 20 1199 20
Size: 1179 3032
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 1175 1231 1175
Size: 56 4155
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 673 856 1227 1641 1889 1924 673
Size: 183 371 414 248 35 2960
Fragments arranged by size:
2960 414 371 248 183 35
BanII G_rGCy'C
Cuts at: 638 1225 2255 638
Size: 587 1030 2594
Fragments arranged by size:
2594 1030 587
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 774 774
Size: 4211
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BglII A'GATC_T
Cuts at: 1726 3763 1726
Size: 2037 2174
BlpI GC'TnA_GC
Cuts at: 517 1323 517
Size: 806 3405
Bme1580I G_kGCm'C
Cuts at: 638 1836 1929 2985 638
Size: 1198 93 1056 1864
Fragments arranged by size:
1864 1198 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 177 1423 1504 1839 3983 177
Size: 1246 81 335 2144 405
Fragments arranged by size:
2144 1246 405 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1002 2578 1002
Size: 1576 2635
Bpu10I CC'TnA_GC
Cuts at: 865 865
Size: 4211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3915 3915
Size: 4211
BsaAI yAC'GTr
Cuts at: 948 1564 948
Size: 616 3595
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 121 242 1642 1890 2592 4088 121
Size: 121 1400 248 702 1496 244
Fragments arranged by size:
1496 1400 702 248 244 121
BsaWI w'CCGG_w
Cuts at: 1400 1921 2599 2873 3020 3431 1400
Size: 521 678 274 147 411 2180
Fragments arranged by size:
2180 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 846 439
Size: 407 3804
BseYI C'CCAG_C
Cuts at: 124 195 1636 2394 2971 3467 124
Size: 71 1441 758 577 496 868
Fragments arranged by size:
1441 868 758 577 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 468
Size: 4211
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 503 665 1225 2003 2193 2985 503
Size: 162 560 778 190 792 1729
Fragments arranged by size:
1729 792 778 560 190 162
Bsp1286I G_dGCh'C
Cuts at: 503 638 665 1225 1836 1929 2003 2193 2255
Size: 135 27 560 611 93 74 190 62
Cuts at: 2255 2985 503
Size: 730 1729
Fragments arranged by size:
1729 730 611 560 190 135 93 74
62 27
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 262 299 1261 1475 1777 2158 262
Size: 37 962 214 302 381 2315
Fragments arranged by size:
2315 962 381 302 214 37
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2123 2123
Size: 4211
BsrFI r'CCGG_y
Cuts at: 425 2209 2390 2599 425
Size: 1784 181 209 2037
Fragments arranged by size:
2037 1784 209 181
BsrGI T'GTAC_A
Cuts at: 1073 1073
Size: 4211
BssSI C'ACGA_G
Cuts at: 664 1024 2482 2840 664
Size: 360 1458 358 2035
Fragments arranged by size:
2035 1458 360 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1588 1588
Size: 4211
BstBI TT'CG_AA
Cuts at: 1127 2572 1127
Size: 1445 2766
BstEII G'GTnAC_C
Cuts at: 570 570
Size: 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 258 306 567 258
Size: 48 261 3902
Fragments arranged by size:
3902 261 48
BstYI r'GATC_y
Cuts at: 1 1236 1703 1726 2061 2307 3319 3405 3417
Size: 1235 467 23 335 246 1012 86 12
Cuts at: 3417 3763 1
Size: 346 449
Fragments arranged by size:
1235 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 494 614 494
Size: 120 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 264 857 2161 2342 2505 264
Size: 593 1304 181 163 1970
Fragments arranged by size:
1970 1304 593 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1454 1112
Size: 342 3869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 696 731 696
Size: 35 4176
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 155 1796 1970 2361 2388 3733 155
Size: 1641 174 391 27 1345 633
Fragments arranged by size:
1641 1345 633 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 125 223 271 466 1045 2234 2444 125
Size: 98 48 195 579 1189 210 1892
Fragments arranged by size:
1892 1189 579 210 195 98 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 843 2419 2729 2875 4021 843
Size: 1576 310 146 1146 1033
Fragments arranged by size:
1576 1146 1033 310 146
EcoICRI GAG'CTC
Cuts at: 1223 1223
Size: 4211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2468 3215 3483 2036
Size: 432 747 268 2764
Fragments arranged by size:
2764 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 864 864
Size: 4211
EcoO109I rG'GnC_Cy
Cuts at: 1350 1350
Size: 4211
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4211
FspI TGC'GCA
Cuts at: 331 1992 331
Size: 1661 2550
HaeII r_GCGC'y
Cuts at: 517 531 632 1645 1893 2915 3894 517
Size: 14 101 1013 248 1022 979 834
Fragments arranged by size:
1022 1013 979 834 248 101 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2053 2083 2085 2115 2053
Size: 30 2 30 4149
Fragments arranged by size:
4149 30 30 2
HincII GTy'rAC
Cuts at: 1250 1250
Size: 4211
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3066 2882
Size: 184 4027
MscI TGG'CCA
Cuts at: 1972 1972
Size: 4211
MslI CAynn'nnrTG
Cuts at: 256 304 643 742 2200 2327 256
Size: 48 339 99 1458 127 2140
Fragments arranged by size:
2140 1458 339 127 99 48
MspA1I CmG'CkG
Cuts at: 231 1322 1636 1996 3009 3254 231
Size: 1091 314 360 1013 245 1188
Fragments arranged by size:
1188 1091 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 4211
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 4211
NspI r_CATG'y
Cuts at: 396 1264 1764 2671 396
Size: 868 500 907 1936
Fragments arranged by size:
1936 907 868 500
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 124 156 3350 3382 124
Size: 32 3194 32 953
Fragments arranged by size:
3194 953 32 32
PspOMI G'GGCC_C
Cuts at: 634 634
Size: 4211
PstI C_TGCA'G
Cuts at: 215 628 1258 1943 215
Size: 413 630 685 2483
Fragments arranged by size:
2483 685 630 413
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 1636 1996 1636
Size: 360 3851
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1225 1225
Size: 4211
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 125 223 2234 2444 125
Size: 98 2011 210 1892
Fragments arranged by size:
2011 1892 210 98
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 1015 3728 1015
Size: 2713 1498
SfcI C'TryA_G
Cuts at: 33 211 624 801 905 1254 1939 2932 3123
Size: 178 413 177 104 349 685 993 191
Cuts at: 3123 33
Size: 1121
Fragments arranged by size:
1121 993 685 413 349 191 178 177
104
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1233 1233
Size: 4211
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 396 1264 1764 396
Size: 868 500 2843
Fragments arranged by size:
2843 868 500
StyI C'CwwG_G
Cuts at: 560 575 682 989 1345 1699 560
Size: 15 107 307 356 354 3072
Fragments arranged by size:
3072 356 354 307 107 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 741 844 1794 1927 2386 2595 741
Size: 103 950 133 459 209 2357
Fragments arranged by size:
2357 950 459 209 133 103
TatI w'GTAC_w
Cuts at: 1013 1073 3726 1013
Size: 60 2653 1498
Fragments arranged by size:
2653 1498 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4179
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1706 1706
Size: 4211
XmaI C'CCGG_G
Cuts at: 1231 1231
Size: 4211
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI