Restriction map for
pF1KE1097
(Circular) (Six-base) MAPSORT of: pF1KE1097.seq Check: 9644 from: 1 to: 4931
pF1KE1097 4931 bp
With 182 enzymes: SgfI *
November 19, 2009 09:54 ..
AccI GT'mk_AC
Cuts at: 1969 1969
Size: 4931
Acc65I G'GTAC_C
Cuts at: 1947 1947
Size: 4931
AclI AA'CG_TT
Cuts at: 4771 4771
Size: 4931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 876 1075 1341 2756 3188 3935 4203 745
Size: 131 199 266 1415 432 747 268 1473
Fragments arranged by size:
1473 1415 747 432 268 266 199 131
AfeI AGC'GCT
Cuts at: 1354 1354
Size: 4931
AflIII A'CryG_T
Cuts at: 2283 3387 4364 2283
Size: 1104 977 2850
Fragments arranged by size:
2850 1104 977
AgeI A'CCGG_T
Cuts at: 3319 3319
Size: 4931
AleI CACnn'nnGTG
Cuts at: 268 268
Size: 4931
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1850 1882 1850
Size: 32 4899
AlwNI CAG_nnn'CTG
Cuts at: 239 429 531 660 2236 3803 239
Size: 190 102 129 1576 1567 1367
Fragments arranged by size:
1576 1567 1367 190 129 102
ApaI G_GGCC'C
Cuts at: 227 1579 1642 1742 1760 1778 227
Size: 1352 63 100 18 18 3380
Fragments arranged by size:
3380 1352 100 63 18 18
ApaLI G'TGCA_C
Cuts at: 766 1219 3701 766
Size: 453 2482 1996
Fragments arranged by size:
2482 1996 453
ApoI r'AATT_y
Cuts at: 521 1935 4284 4540 521
Size: 1414 2349 256 912
Fragments arranged by size:
2349 1414 912 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 1652 1761 1887 1951 1652
Size: 109 126 64 4632
Fragments arranged by size:
4632 126 109 64
BamHI G'GATC_C
Cuts at: 432 806 815 1865 1956 432
Size: 374 9 1050 91 3407
Fragments arranged by size:
3407 1050 374 91 9
BanI G'GyrC_C
Cuts at: 1180 1330 1477 1947 2361 2609 2644 1180
Size: 150 147 470 414 248 35 3467
Fragments arranged by size:
3467 470 414 248 150 147 35
BanII G_rGCy'C
Cuts at: 227 668 1295 1579 1642 1742 1760 1778 1945
Size: 441 627 284 63 100 18 18 167
Cuts at: 1945 2975 227
Size: 1030 2183
Fragments arranged by size:
2183 1030 627 441 284 167 100 63
18 18
BbeI G_GCGC'C
Cuts at: 2365 2613 2365
Size: 248 4683
BbsI GAAGACnn'nnnn_
Cuts at: 368 832 1819 368
Size: 464 987 3480
Fragments arranged by size:
3480 987 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 121 155 121
Size: 34 4897
BciVI GTATCCnnnnn_n'
Cuts at: 2829 3590 4931 2829
Size: 761 1341 2829
Fragments arranged by size:
2829 1341 761
BclI T'GATC_A
Cuts at: 2451 2451
Size: 4931
BfrBI ATG'CAT
Cuts at: 1083 1083
Size: 4931
BglI GCCn_nnn'nGGC
Cuts at: 1646 1673 1746 1755 1782 1646
Size: 27 73 9 27 4795
Fragments arranged by size:
4795 73 27 27 9
BglII A'GATC_T
Cuts at: 2446 4483 2446
Size: 2037 2894
BlpI GC'TnA_GC
Cuts at: 2043 2043
Size: 4931
Bme1580I G_kGCm'C
Cuts at: 227 770 1223 1333 1374 1482 1579 1642 1742
Size: 543 453 110 41 108 97 63 100
Cuts at: 1742 1760 1778 2556 2649 3705 227
Size: 18 18 778 93 1056 1453
Fragments arranged by size:
1453 1056 778 543 453 110 108 100
97 93 63 41 18 18
BmgBI CAC'GTC
Cuts at: 1248 1248
Size: 4931
BmrI ACTGGGnnnn_n'
Cuts at: 372 2143 2224 2559 4703 372
Size: 1771 81 335 2144 600
Fragments arranged by size:
2144 1771 600 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 603 3298 603
Size: 2695 2236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 535 835 944 997 1475 1631 1718 1754
Size: 129 300 109 53 478 156 87 36
Cuts at: 1754 1880 406
Size: 126 3457
Fragments arranged by size:
3457 478 300 156 129 126 109 87
53 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2107 2986 3478 3776 1216
Size: 891 879 492 298 2371
Fragments arranged by size:
2371 891 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 832 1553 1772 1888 4635 832
Size: 721 219 116 2747 1128
Fragments arranged by size:
2747 1128 721 219 116
BsaAI yAC'GTr
Cuts at: 1218 2284 1218
Size: 1066 3865
BsaBI GATnn'nnATC
Cuts at: 6 2445 4482 6
Size: 2439 2037 455
Fragments arranged by size:
2439 2037 455
BsaHI Gr'CG_yC
Cuts at: 2362 2610 3312 4808 2362
Size: 248 702 1496 2485
Fragments arranged by size:
2485 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1391 2120 2641 3319 3593 3740 4151 1391
Size: 729 521 678 274 147 411 2171
Fragments arranged by size:
2171 729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 917 947 340
Size: 30 547 30 4324
Fragments arranged by size:
4324 547 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 850 954 1303 1429 523
Size: 327 104 349 126 4025
Fragments arranged by size:
4025 349 327 126 104
BseYI C'CCAG_C
Cuts at: 472 480 1266 1326 2356 3114 3691 4187 472
Size: 8 786 60 1030 758 577 496 1216
Fragments arranged by size:
1216 1030 786 758 577 496 60 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1245 571
Size: 674 4257
BsiEI CG_ry'CG
Cuts at: 104 125 2473 2519 3727 104
Size: 21 2348 46 1208 1308
Fragments arranged by size:
2348 1308 1208 46 21
BsiHKAI G_wGCw'C
Cuts at: 587 770 1223 1295 1945 2723 2913 3705 587
Size: 183 453 72 650 778 190 792 1813
Fragments arranged by size:
1813 792 778 650 453 190 183 72
Bsp1286I G_dGCh'C
Cuts at: 227 587 668 770 1223 1295 1333 1374 1482
Size: 360 81 102 453 72 38 41 108
Cuts at: 1482 1579 1642 1742 1760 1778 1945 2556 2649
Size: 97 63 100 18 18 167 611 93
Cuts at: 2649 2723 2913 2975 3705 227
Size: 74 190 62 730 1453
Fragments arranged by size:
1453 730 611 453 360 190 167 108
102 100 97 93 81 74 72 63
62 41 38 18 18
BspEI T'CCGG_A
Cuts at: 1391 4151 1391
Size: 2760 2171
BspHI T'CATG_A
Cuts at: 4107 4107
Size: 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1349 1981 2195 2497 2878 1349
Size: 632 214 302 381 3402
Fragments arranged by size:
3402 632 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3223 3277 4761 4926 50
Size: 3173 54 1484 165 55
Fragments arranged by size:
3173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1464 2843 1464
Size: 1379 3552
BsrFI r'CCGG_y
Cuts at: 124 215 2929 3110 3319 124
Size: 91 2714 181 209 1736
Fragments arranged by size:
2714 1736 209 181 91
BssSI C'ACGA_G
Cuts at: 1165 3202 3560 1165
Size: 2037 358 2536
Fragments arranged by size:
2536 2037 358
BstAPI GCAn_nnn'nTGC
Cuts at: 239 1227 2308 239
Size: 988 1081 2862
Fragments arranged by size:
2862 1081 988
BstBI TT'CG_AA
Cuts at: 3292 3292
Size: 4931
BstEII G'GTnAC_C
Cuts at: 1820 1820
Size: 4931
BstXI CCAn_nnnn'nTGG
Cuts at: 472 1326 1410 472
Size: 854 84 3993
Fragments arranged by size:
3993 854 84
BstYI r'GATC_y
Cuts at: 1 432 491 806 815 1865 1956 2423 2446
Size: 431 59 315 9 1050 91 467 23
Cuts at: 2446 2781 3027 4039 4125 4137 4483 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1050 1012 467 449 431 346 335 315
246 91 86 59 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 1598 1779 1787 1850 1598
Size: 181 8 63 4679
Fragments arranged by size:
4679 181 63 8
BtgI C'CryG_G
Cuts at: 311 785 810 1841 311
Size: 474 25 1031 3401
Fragments arranged by size:
3401 1031 474 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 146 2881 3062 3225 146
Size: 2735 181 163 1852
Fragments arranged by size:
2735 1852 181 163
BtsI GCAGTG_nn'
Cuts at: 770 1433 2174 770
Size: 663 741 3527
Fragments arranged by size:
3527 741 663
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4931
DrdI GACnn_nn'nnGTC
Cuts at: 1387 1592 2637 3495 1387
Size: 205 1045 858 2823
Fragments arranged by size:
2823 1045 858 205
EaeI y'GGCC_r
Cuts at: 122 217 308 2516 2690 3081 3108 4453 122
Size: 95 91 2208 174 391 27 1345 600
Fragments arranged by size:
2208 1345 600 391 174 95 91 27
EagI C'GGCC_G
Cuts at: 122 2516 122
Size: 2394 2537
EarI CTCTTCn'nnn_
Cuts at: 152 292 673 895 1067 2954 3164 152
Size: 140 381 222 172 1887 210 1919
Fragments arranged by size:
1919 1887 381 222 210 172 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3449 3595 4741 3139
Size: 310 146 1146 3329
Fragments arranged by size:
3329 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1293 1943 1293
Size: 650 4281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 535 745 835 876 944 997 1075 1341
Size: 129 210 90 41 68 53 78 266
Cuts at: 1341 1475 1631 1718 1754 1880 2756 3188 3935
Size: 134 156 87 36 126 876 432 747
Cuts at: 3935 4203 406
Size: 268 1134
Fragments arranged by size:
1134 876 747 432 268 266 210 156
134 129 126 90 87 78 68 53
41 36
EcoNI CCTnn'n_nnAGG
Cuts at: 676 736 676
Size: 60 4871
EcoO109I rG'GnC_Cy
Cuts at: 162 223 224 426 729 959 1531 1576 1585
Size: 61 1 202 303 230 572 45 9
Cuts at: 1585 1594 1638 1639 1738 1739 1756 1757 1774
Size: 9 44 1 99 1 17 1 17
Cuts at: 1774 1775 1783 1846 1873 1891 2070 162
Size: 1 8 63 27 18 179 3023
Fragments arranged by size:
3023 572 303 230 202 179 99 63
61 45 44 27 18 17 17 9
9 8 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 521 1935 521
Size: 1414 3517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1704 2950 1704
Size: 1246 3685
FseI GG_CCGG'CC
Cuts at: 219 219
Size: 4931
FspI TGC'GCA
Cuts at: 904 2712 904
Size: 1808 3123
HaeII r_GCGC'y
Cuts at: 1356 2365 2613 3635 4614 1356
Size: 1009 248 1022 979 1673
Fragments arranged by size:
1673 1022 1009 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 340 372 917 949 1033 1065 1857 1889 2773
Size: 32 545 32 84 32 792 32 884
Cuts at: 2773 2803 2805 2835 340
Size: 30 2 30 2436
Fragments arranged by size:
2436 884 792 545 84 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 651 1970 651
Size: 1319 3612
HindIII A'AGCT_T
Cuts at: 397 397
Size: 4931
HpaI GTT'AAC
Cuts at: 651 651
Size: 4931
KasI G'GCGC_C
Cuts at: 2361 2609 2361
Size: 248 4683
KpnI G_GTAC'C
Cuts at: 1951 1951
Size: 4931
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3602 3786 3602
Size: 184 4747
MscI TGG'CCA
Cuts at: 2692 2692
Size: 4931
MslI CAynn'nnrTG
Cuts at: 112 268 1280 1408 2920 3047 112
Size: 156 1012 128 1512 127 1996
Fragments arranged by size:
1996 1512 1012 156 128 127
MspA1I CmG'CkG
Cuts at: 230 239 480 555 706 822 896 1032 1038
Size: 9 241 75 151 116 74 136 6
Cuts at: 1038 1833 2042 2356 2716 3729 3974 230
Size: 795 209 314 360 1013 245 1187
Fragments arranged by size:
1187 1013 795 360 314 245 241 209
151 136 116 75 74 9 6
NaeI GCC'GGC
Cuts at: 126 217 3112 126
Size: 91 2895 1945
Fragments arranged by size:
2895 1945 91
NarI GG'CG_CC
Cuts at: 2362 2610 2362
Size: 248 4683
NcoI C'CATG_G
Cuts at: 1841 1841
Size: 4931
NgoMIV G'CCGG_C
Cuts at: 124 215 3110 124
Size: 91 2895 1945
Fragments arranged by size:
2895 1945 91
NsiI A_TGCA'T
Cuts at: 1085 1085
Size: 4931
NspI r_CATG'y
Cuts at: 189 999 1083 1230 1984 2484 3391 189
Size: 810 84 147 754 500 907 1729
Fragments arranged by size:
1729 907 810 754 500 147 84
PasI CC'CwG_GG
Cuts at: 954 1581 1590 1744 954
Size: 627 9 154 4141
Fragments arranged by size:
4141 627 154 9
PciI A'CATG_T
Cuts at: 3387 3387
Size: 4931
PflMI CCAn_nnn'nTGG
Cuts at: 429 618 660 1020 429
Size: 189 42 360 4340
Fragments arranged by size:
4340 360 189 42
PfoI T'CCnGG_A
Cuts at: 1058 1346 1491 1058
Size: 288 145 4498
Fragments arranged by size:
4498 288 145
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PmlI CAC'GTG
Cuts at: 1218 1218
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 439 471 4070 4102 439
Size: 32 3599 32 1268
Fragments arranged by size:
3599 1268 32 32
PpuMI rG'GwC_Cy
Cuts at: 162 729 1585 1594 1873 162
Size: 567 856 9 279 3220
Fragments arranged by size:
3220 856 567 279 9
PspOMI G'GGCC_C
Cuts at: 223 1575 1638 1738 1756 1774 223
Size: 1352 63 100 18 18 3380
Fragments arranged by size:
3380 1352 100 63 18 18
PspXI vC'TCGA_Gb
Cuts at: 1652 1652
Size: 4931
PstI C_TGCA'G
Cuts at: 235 506 765 1037 1978 2663 235
Size: 271 259 272 941 685 2503
Fragments arranged by size:
2503 941 685 272 271 259
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 480 555 706 1032 1038 2356 2716 480
Size: 75 151 326 6 1318 360 2695
Fragments arranged by size:
2695 1318 360 326 151 75 6
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 1295 1945 1295
Size: 650 4281
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SanDI GG'GwC_CC
Cuts at: 1585 1873 1585
Size: 288 4643
SapI GCTCTTCn'nnn_
Cuts at: 673 895 2954 3164 673
Size: 222 2059 210 2440
Fragments arranged by size:
2440 2059 222 210
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 4448 4448
Size: 4931
SexAI A'CCwGG_T
Cuts at: 644 1004 644
Size: 360 4571
SfcI C'TryA_G
Cuts at: 33 231 502 761 1033 1974 2659 3652 3843
Size: 198 271 259 272 941 685 993 191
Cuts at: 3843 33
Size: 1121
Fragments arranged by size:
1121 993 941 685 272 271 259 198
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1646 1746 1755 1782 1646
Size: 100 9 27 4795
Fragments arranged by size:
4795 100 27 9
SfoI GGC'GCC
Cuts at: 2363 2611 2363
Size: 248 4683
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 1763 1889 1953 1763
Size: 126 64 4741
Fragments arranged by size:
4741 126 64
SmlI C'TyrA_G
Cuts at: 1231 1652 2086 3001 3493 3755 1231
Size: 421 434 915 492 262 2407
Fragments arranged by size:
2407 915 492 434 421 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4931
SphI G_CATG'C
Cuts at: 999 1230 1984 2484 999
Size: 231 754 500 3446
Fragments arranged by size:
3446 754 500 231
StuI AGG'CCT
Cuts at: 1667 1667
Size: 4931
StyI C'CwwG_G
Cuts at: 1841 2065 2419 1841
Size: 224 354 4353
Fragments arranged by size:
4353 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 751 1294 2514 2647 3106 3315 220
Size: 531 543 1220 133 459 209 1836
Fragments arranged by size:
1836 1220 543 531 459 209 133
TatI w'GTAC_w
Cuts at: 4446 4446
Size: 4931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 4899
Tth111I GACn'n_nGTC
Cuts at: 378 1818 2728 378
Size: 1440 910 2581
Fragments arranged by size:
2581 1440 910
XbaI T'CTAG_A
Cuts at: 1962 1962
Size: 4931
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 1876 2426 708
Size: 1168 550 3213
Fragments arranged by size:
3213 1168 550
XhoI C'TCGA_G
Cuts at: 1652 1652
Size: 4931
XmaI C'CCGG_G
Cuts at: 1761 1887 1951 1761
Size: 126 64 4741
Fragments arranged by size:
4741 126 64
XmnI GAAnn'nnTTC
Cuts at: 1451 1451
Size: 4931
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FspAI MfeI NdeI
NheI NotI NruI PacI PshAI PsiI PsrI SacII
SgrAI SnaBI SrfI SspI SwaI ZraI