Restriction map for pF1KE1098
 (Circular) (Six-base) MAPSORT of: pF1KE1098.seq  Check: 1360  from: 1  to: 4058



pF1KE1098  4058 bp

 With 182 enzymes: SgfI * 

                        November 19, 2009 09:54  ..

AccI GT'mk_AC

Cuts at:   1096    1096 
   Size:       4058
 
Acc65I G'GTAC_C

Cuts at:   1074    1074 
   Size:       4058
 
AclI AA'CG_TT

Cuts at:   3898    3898 
   Size:       4058
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1067    1883    2315    3062    3330    1067 
   Size:        816     432     747     268    1795

  Fragments arranged by size: 

                1795     816     747     432     268
 
AflIII A'CryG_T

Cuts at:   1410    2514    3491    1410 
   Size:       1104     977    1977

  Fragments arranged by size: 

                1977    1104     977
 
AgeI A'CCGG_T

Cuts at:   2446    2446 
   Size:       4058
 
AlwNI CAG_nnn'CTG

Cuts at:   1363    2930    1363 
   Size:       1567    2491
 
ApaLI G'TGCA_C

Cuts at:   2828    2828 
   Size:       4058
 
ApoI r'AATT_y

Cuts at:    142     409     677    1062    3411    3667     142 
   Size:        267     268     385    2349     256     533

  Fragments arranged by size: 

                2349     533     385     268     267     256
 
AseI AT'TA_AT

Cuts at:     20     638     647     788      20 
   Size:        618       9     141    3290

  Fragments arranged by size: 

                3290     618     141       9
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4058
 
AvaI C'yCGr_G

Cuts at:   1078    1078 
   Size:       4058
 
BamHI G'GATC_C

Cuts at:   1083    1083 
   Size:       4058
 
BanI G'GyrC_C

Cuts at:    550    1074    1488    1736    1771     550 
   Size:        524     414     248      35    2837

  Fragments arranged by size: 

                2837     524     414     248      35
 
BanII G_rGCy'C

Cuts at:    932    1072    2102     932 
   Size:        140    1030    2888

  Fragments arranged by size: 

                2888    1030     140
 
BbeI G_GCGC'C

Cuts at:   1492    1740    1492 
   Size:        248    3810
 
BbsI GAAGACnn'nnnn_

Cuts at:    410     410 
   Size:       4058
 
BbvCI CC'TCA_GC

Cuts at:    455     455 
   Size:       4058
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4024
 
BciVI GTATCCnnnnn_n'

Cuts at:    283    1956    2717    4058     283 
   Size:       1673     761    1341     283

  Fragments arranged by size: 

                1673    1341     761     283
 
BclI T'GATC_A

Cuts at:   1578    1578 
   Size:       4058
 
BfrBI ATG'CAT

Cuts at:    562     562 
   Size:       4058
 
BglII A'GATC_T

Cuts at:   1573    3610    1573 
   Size:       2037    2021
 
BlpI GC'TnA_GC

Cuts at:   1170    1170 
   Size:       4058
 
Bme1580I G_kGCm'C

Cuts at:   1683    1776    2832    1683 
   Size:         93    1056    2909

  Fragments arranged by size: 

                2909    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1270    1351    1686    3830    1270 
   Size:         81     335    2144    1498

  Fragments arranged by size: 

                2144    1498     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2425    2425 
   Size:       4058
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    365     853     365 
   Size:        488    3570
 
Bpu10I CC'TnA_GC

Cuts at:    455     455 
   Size:       4058
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1234    2113    2605    2903    1234 
   Size:        879     492     298    2389

  Fragments arranged by size: 

                2389     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    531    3762     531 
   Size:       3231     827
 
BsaAI yAC'GTr

Cuts at:   1411    1411 
   Size:       4058
 
BsaBI GATnn'nnATC

Cuts at:      6    1572    3609       6 
   Size:       1566    2037     455

  Fragments arranged by size: 

                2037    1566     455
 
BsaHI Gr'CG_yC

Cuts at:   1489    1737    2439    3935    1489 
   Size:        248     702    1496    1612

  Fragments arranged by size: 

                1612    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1247    1768    2446    2720    2867    3278    1247 
   Size:        521     678     274     147     411    2027

  Fragments arranged by size: 

                2027     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    791     794     791 
   Size:          3    4055
 
BseYI C'CCAG_C

Cuts at:   1483    2241    2818    3314    1483 
   Size:        758     577     496    2227

  Fragments arranged by size: 

                2227     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1600    1646    2854     104 
   Size:       1496      46    1208    1308

  Fragments arranged by size: 

                1496    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1072    1850    2040    2832    1072 
   Size:        778     190     792    2298

  Fragments arranged by size: 

                2298     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    437     437 
   Size:       4058
 
Bsp1286I G_dGCh'C

Cuts at:    932    1072    1683    1776    1850    2040    2102    2832     932 
   Size:        140     611      93      74     190      62     730    2158

  Fragments arranged by size: 

                2158     730     611     190     140      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3278    3278 
   Size:       4058
 
BspHI T'CATG_A

Cuts at:   3234    3234 
   Size:       4058
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    739    1108    1322    1624    2005     739 
   Size:        369     214     302     381    2792

  Fragments arranged by size: 

                2792     381     369     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2350    2404    3888    4053      50 
   Size:       2300      54    1484     165      55

  Fragments arranged by size: 

                2300    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    185    1970     185 
   Size:       1785    2273
 
BsrFI r'CCGG_y

Cuts at:   2056    2237    2446    2056 
   Size:        181     209    3668

  Fragments arranged by size: 

                3668     209     181
 
BssSI C'ACGA_G

Cuts at:   2329    2687    2329 
   Size:        358    3700
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1435    1435 
   Size:       4058
 
BstBI TT'CG_AA

Cuts at:   2419    2419 
   Size:       4058
 
BstYI r'GATC_y

Cuts at:      1     181    1083    1550    1573    1908    2154    3166    3252 
   Size:        180     902     467      23     335     246    1012      86

Cuts at:   3252    3264    3610       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     902     467     449     346     335     246     180
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    149     149 
   Size:       4058
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2008    2189    2352    2008 
   Size:        181     163    3714

  Fragments arranged by size: 

                3714     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1301    1301 
   Size:       4058
 
DraI TTT'AAA

Cuts at:   1057    1057 
   Size:       4058
 
DrdI GACnn_nn'nnGTC

Cuts at:   1764    2622    1764 
   Size:        858    3200
 
EaeI y'GGCC_r

Cuts at:   1643    1817    2208    2235    3580    1643 
   Size:        174     391      27    1345    2121

  Fragments arranged by size: 

                2121    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1643    1643 
   Size:       4058
 
EarI CTCTTCn'nnn_

Cuts at:    366    2081    2291     366 
   Size:       1715     210    2133

  Fragments arranged by size: 

                2133    1715     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2266    2576    2722    3868    2266 
   Size:        310     146    1146    2456

  Fragments arranged by size: 

                2456    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1070    1070 
   Size:       4058
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    365     853    1067    1883    2315    3062    3330     365 
   Size:        488     214     816     432     747     268    1093

  Fragments arranged by size: 

                1093     816     747     488     432     268     214
 
EcoO109I rG'GnC_Cy

Cuts at:   1197    1197 
   Size:       4058
 
EcoRI G'AATT_C

Cuts at:   1062    1062 
   Size:       4058
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2077    2077 
   Size:       4058
 
FspI TGC'GCA

Cuts at:   1839    1839 
   Size:       4058
 
HaeII r_GCGC'y

Cuts at:    657    1492    1740    2762    3741     657 
   Size:        835     248    1022     979     974

  Fragments arranged by size: 

                1022     979     974     835     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1900    1930    1932    1962    1900 
   Size:         30       2      30    3996

  Fragments arranged by size: 

                3996      30      30       2
 
HincII GTy'rAC

Cuts at:   1097    1097 
   Size:       4058
 
KasI G'GCGC_C

Cuts at:   1488    1736    1488 
   Size:        248    3810
 
KpnI G_GTAC'C

Cuts at:   1078    1078 
   Size:       4058
 
MluI A'CGCG_T

Cuts at:   3491    3491 
   Size:       4058
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2729    2913    2729 
   Size:        184    3874
 
MscI TGG'CCA

Cuts at:   1819    1819 
   Size:       4058
 
MslI CAynn'nnrTG

Cuts at:    331    2047    2174     331 
   Size:       1716     127    2215

  Fragments arranged by size: 

                2215    1716     127
 
MspA1I CmG'CkG

Cuts at:    486    1169    1483    1843    2856    3101     486 
   Size:        683     314     360    1013     245    1443

  Fragments arranged by size: 

                1443    1013     683     360     314     245
 
NaeI GCC'GGC

Cuts at:   2239    2239 
   Size:       4058
 
NarI GG'CG_CC

Cuts at:   1489    1737    1489 
   Size:        248    3810
 
NgoMIV G'CCGG_C

Cuts at:   2237    2237 
   Size:       4058
 
NsiI A_TGCA'T

Cuts at:    564     564 
   Size:       4058
 
NspI r_CATG'y

Cuts at:    574     777    1111    1611    2518     574 
   Size:        203     334     500     907    2114

  Fragments arranged by size: 

                2114     907     500     334     203
 
PciI A'CATG_T

Cuts at:   2514    2514 
   Size:       4058
 
PmeI GTTT'AAAC

Cuts at:   1057    1057 
   Size:       4058
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3197    3229    3197 
   Size:         32    4026
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    614     646     614 
   Size:         32    4026
 
PstI C_TGCA'G

Cuts at:   1105    1790    1105 
   Size:        685    3373
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4058
 
PvuII CAG'CTG

Cuts at:    486    1483    1843     486 
   Size:        997     360    2701

  Fragments arranged by size: 

                2701     997     360
 
RsrII CG'GwC_CG

Cuts at:   2253    2253 
   Size:       4058
 
SacI G_AGCT'C

Cuts at:   1072    1072 
   Size:       4058
 
SalI G'TCGA_C

Cuts at:   1095    1095 
   Size:       4058
 
SapI GCTCTTCn'nnn_

Cuts at:   2081    2291    2081 
   Size:        210    3848
 
SbfI CC_TGCA'GG

Cuts at:   1105    1105 
   Size:       4058
 
ScaI AGT'ACT

Cuts at:    632    3575     632 
   Size:       2943    1115
 
SfcI C'TryA_G

Cuts at:     33     764    1101    1786    2779    2970      33 
   Size:        731     337     685     993     191    1121

  Fragments arranged by size: 

                1121     993     731     685     337     191
 
SfoI GGC'GCC

Cuts at:   1490    1738    1490 
   Size:        248    3810
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4058
 
SmaI CCC'GGG

Cuts at:   1080    1080 
   Size:       4058
 
SmlI C'TyrA_G

Cuts at:   1213    2128    2620    2882    1213 
   Size:        915     492     262    2389

  Fragments arranged by size: 

                2389     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4058
 
SphI G_CATG'C

Cuts at:    574    1111    1611     574 
   Size:        537     500    3021

  Fragments arranged by size: 

                3021     537     500
 
SspI AAT'ATT

Cuts at:    645     645 
   Size:       4058
 
StuI AGG'CCT

Cuts at:    848     848 
   Size:       4058
 
StyI C'CwwG_G

Cuts at:    473    1192    1546     473 
   Size:        719     354    2985

  Fragments arranged by size: 

                2985     719     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1641    1774    2233    2442    1641 
   Size:        133     459     209    3257

  Fragments arranged by size: 

                3257     459     209     133
 
TatI w'GTAC_w

Cuts at:    530     630    3573     530 
   Size:        100    2943    1015

  Fragments arranged by size: 

                2943    1015     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    300     332    3575    3607     300 
   Size:         32    3243      32     751

  Fragments arranged by size: 

                3243     751      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1855    1855 
   Size:       4058
 
XbaI T'CTAG_A

Cuts at:   1089    1089 
   Size:       4058
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    192    1553     192 
   Size:       1361    2697
 
XmaI C'CCGG_G

Cuts at:   1078    1078 
   Size:       4058
 
XmnI GAAnn'nnTTC

Cuts at:    375     375 
   Size:       4058
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI
     NspI     PciI     PmeI     PpiI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BglI    BmgBI     BmtI    BsaXI
     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I
     BtgI     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SwaI     XhoI     ZraI
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