(Circular) (Six-base) MAPSORT of: pF1KE1098.seq Check: 1360 from: 1 to: 4058 pF1KE1098 4058 bp With 182 enzymes: SgfI * November 19, 2009 09:54 .. AccI GT'mk_AC Cuts at: 1096 1096 Size: 4058 Acc65I G'GTAC_C Cuts at: 1074 1074 Size: 4058 AclI AA'CG_TT Cuts at: 3898 3898 Size: 4058 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1067 1883 2315 3062 3330 1067 Size: 816 432 747 268 1795 Fragments arranged by size: 1795 816 747 432 268 AflIII A'CryG_T Cuts at: 1410 2514 3491 1410 Size: 1104 977 1977 Fragments arranged by size: 1977 1104 977 AgeI A'CCGG_T Cuts at: 2446 2446 Size: 4058 AlwNI CAG_nnn'CTG Cuts at: 1363 2930 1363 Size: 1567 2491 ApaLI G'TGCA_C Cuts at: 2828 2828 Size: 4058 ApoI r'AATT_y Cuts at: 142 409 677 1062 3411 3667 142 Size: 267 268 385 2349 256 533 Fragments arranged by size: 2349 533 385 268 267 256 AseI AT'TA_AT Cuts at: 20 638 647 788 20 Size: 618 9 141 3290 Fragments arranged by size: 3290 618 141 9 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4058 AvaI C'yCGr_G Cuts at: 1078 1078 Size: 4058 BamHI G'GATC_C Cuts at: 1083 1083 Size: 4058 BanI G'GyrC_C Cuts at: 550 1074 1488 1736 1771 550 Size: 524 414 248 35 2837 Fragments arranged by size: 2837 524 414 248 35 BanII G_rGCy'C Cuts at: 932 1072 2102 932 Size: 140 1030 2888 Fragments arranged by size: 2888 1030 140 BbeI G_GCGC'C Cuts at: 1492 1740 1492 Size: 248 3810 BbsI GAAGACnn'nnnn_ Cuts at: 410 410 Size: 4058 BbvCI CC'TCA_GC Cuts at: 455 455 Size: 4058 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4024 BciVI GTATCCnnnnn_n' Cuts at: 283 1956 2717 4058 283 Size: 1673 761 1341 283 Fragments arranged by size: 1673 1341 761 283 BclI T'GATC_A Cuts at: 1578 1578 Size: 4058 BfrBI ATG'CAT Cuts at: 562 562 Size: 4058 BglII A'GATC_T Cuts at: 1573 3610 1573 Size: 2037 2021 BlpI GC'TnA_GC Cuts at: 1170 1170 Size: 4058 Bme1580I G_kGCm'C Cuts at: 1683 1776 2832 1683 Size: 93 1056 2909 Fragments arranged by size: 2909 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1270 1351 1686 3830 1270 Size: 81 335 2144 1498 Fragments arranged by size: 2144 1498 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 4058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 365 853 365 Size: 488 3570 Bpu10I CC'TnA_GC Cuts at: 455 455 Size: 4058 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1234 2113 2605 2903 1234 Size: 879 492 298 2389 Fragments arranged by size: 2389 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 531 3762 531 Size: 3231 827 BsaAI yAC'GTr Cuts at: 1411 1411 Size: 4058 BsaBI GATnn'nnATC Cuts at: 6 1572 3609 6 Size: 1566 2037 455 Fragments arranged by size: 2037 1566 455 BsaHI Gr'CG_yC Cuts at: 1489 1737 2439 3935 1489 Size: 248 702 1496 1612 Fragments arranged by size: 1612 1496 702 248 BsaWI w'CCGG_w Cuts at: 1247 1768 2446 2720 2867 3278 1247 Size: 521 678 274 147 411 2027 Fragments arranged by size: 2027 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 791 794 791 Size: 3 4055 BseYI C'CCAG_C Cuts at: 1483 2241 2818 3314 1483 Size: 758 577 496 2227 Fragments arranged by size: 2227 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1600 1646 2854 104 Size: 1496 46 1208 1308 Fragments arranged by size: 1496 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1072 1850 2040 2832 1072 Size: 778 190 792 2298 Fragments arranged by size: 2298 792 778 190 BsmI GAATG_Cn' Cuts at: 437 437 Size: 4058 Bsp1286I G_dGCh'C Cuts at: 932 1072 1683 1776 1850 2040 2102 2832 932 Size: 140 611 93 74 190 62 730 2158 Fragments arranged by size: 2158 730 611 190 140 93 74 62 BspEI T'CCGG_A Cuts at: 3278 3278 Size: 4058 BspHI T'CATG_A Cuts at: 3234 3234 Size: 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 739 1108 1322 1624 2005 739 Size: 369 214 302 381 2792 Fragments arranged by size: 2792 381 369 302 214 BsrBI CCG'CTC Cuts at: 50 2350 2404 3888 4053 50 Size: 2300 54 1484 165 55 Fragments arranged by size: 2300 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 185 1970 185 Size: 1785 2273 BsrFI r'CCGG_y Cuts at: 2056 2237 2446 2056 Size: 181 209 3668 Fragments arranged by size: 3668 209 181 BssSI C'ACGA_G Cuts at: 2329 2687 2329 Size: 358 3700 BstAPI GCAn_nnn'nTGC Cuts at: 1435 1435 Size: 4058 BstBI TT'CG_AA Cuts at: 2419 2419 Size: 4058 BstYI r'GATC_y Cuts at: 1 181 1083 1550 1573 1908 2154 3166 3252 Size: 180 902 467 23 335 246 1012 86 Cuts at: 3252 3264 3610 1 Size: 12 346 449 Fragments arranged by size: 1012 902 467 449 346 335 246 180 86 23 12 Bsu36I CC'TnA_GG Cuts at: 149 149 Size: 4058 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2008 2189 2352 2008 Size: 181 163 3714 Fragments arranged by size: 3714 181 163 BtsI GCAGTG_nn' Cuts at: 1301 1301 Size: 4058 DraI TTT'AAA Cuts at: 1057 1057 Size: 4058 DrdI GACnn_nn'nnGTC Cuts at: 1764 2622 1764 Size: 858 3200 EaeI y'GGCC_r Cuts at: 1643 1817 2208 2235 3580 1643 Size: 174 391 27 1345 2121 Fragments arranged by size: 2121 1345 391 174 27 EagI C'GGCC_G Cuts at: 1643 1643 Size: 4058 EarI CTCTTCn'nnn_ Cuts at: 366 2081 2291 366 Size: 1715 210 2133 Fragments arranged by size: 2133 1715 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2266 2576 2722 3868 2266 Size: 310 146 1146 2456 Fragments arranged by size: 2456 1146 310 146 EcoICRI GAG'CTC Cuts at: 1070 1070 Size: 4058 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 365 853 1067 1883 2315 3062 3330 365 Size: 488 214 816 432 747 268 1093 Fragments arranged by size: 1093 816 747 488 432 268 214 EcoO109I rG'GnC_Cy Cuts at: 1197 1197 Size: 4058 EcoRI G'AATT_C Cuts at: 1062 1062 Size: 4058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 4058 FspI TGC'GCA Cuts at: 1839 1839 Size: 4058 HaeII r_GCGC'y Cuts at: 657 1492 1740 2762 3741 657 Size: 835 248 1022 979 974 Fragments arranged by size: 1022 979 974 835 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1900 1930 1932 1962 1900 Size: 30 2 30 3996 Fragments arranged by size: 3996 30 30 2 HincII GTy'rAC Cuts at: 1097 1097 Size: 4058 KasI G'GCGC_C Cuts at: 1488 1736 1488 Size: 248 3810 KpnI G_GTAC'C Cuts at: 1078 1078 Size: 4058 MluI A'CGCG_T Cuts at: 3491 3491 Size: 4058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2729 2913 2729 Size: 184 3874 MscI TGG'CCA Cuts at: 1819 1819 Size: 4058 MslI CAynn'nnrTG Cuts at: 331 2047 2174 331 Size: 1716 127 2215 Fragments arranged by size: 2215 1716 127 MspA1I CmG'CkG Cuts at: 486 1169 1483 1843 2856 3101 486 Size: 683 314 360 1013 245 1443 Fragments arranged by size: 1443 1013 683 360 314 245 NaeI GCC'GGC Cuts at: 2239 2239 Size: 4058 NarI GG'CG_CC Cuts at: 1489 1737 1489 Size: 248 3810 NgoMIV G'CCGG_C Cuts at: 2237 2237 Size: 4058 NsiI A_TGCA'T Cuts at: 564 564 Size: 4058 NspI r_CATG'y Cuts at: 574 777 1111 1611 2518 574 Size: 203 334 500 907 2114 Fragments arranged by size: 2114 907 500 334 203 PciI A'CATG_T Cuts at: 2514 2514 Size: 4058 PmeI GTTT'AAAC Cuts at: 1057 1057 Size: 4058 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3197 3229 3197 Size: 32 4026 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 614 646 614 Size: 32 4026 PstI C_TGCA'G Cuts at: 1105 1790 1105 Size: 685 3373 PvuI CG_AT'CG Cuts at: 104 104 Size: 4058 PvuII CAG'CTG Cuts at: 486 1483 1843 486 Size: 997 360 2701 Fragments arranged by size: 2701 997 360 RsrII CG'GwC_CG Cuts at: 2253 2253 Size: 4058 SacI G_AGCT'C Cuts at: 1072 1072 Size: 4058 SalI G'TCGA_C Cuts at: 1095 1095 Size: 4058 SapI GCTCTTCn'nnn_ Cuts at: 2081 2291 2081 Size: 210 3848 SbfI CC_TGCA'GG Cuts at: 1105 1105 Size: 4058 ScaI AGT'ACT Cuts at: 632 3575 632 Size: 2943 1115 SfcI C'TryA_G Cuts at: 33 764 1101 1786 2779 2970 33 Size: 731 337 685 993 191 1121 Fragments arranged by size: 1121 993 731 685 337 191 SfoI GGC'GCC Cuts at: 1490 1738 1490 Size: 248 3810 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4058 SmaI CCC'GGG Cuts at: 1080 1080 Size: 4058 SmlI C'TyrA_G Cuts at: 1213 2128 2620 2882 1213 Size: 915 492 262 2389 Fragments arranged by size: 2389 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4058 SphI G_CATG'C Cuts at: 574 1111 1611 574 Size: 537 500 3021 Fragments arranged by size: 3021 537 500 SspI AAT'ATT Cuts at: 645 645 Size: 4058 StuI AGG'CCT Cuts at: 848 848 Size: 4058 StyI C'CwwG_G Cuts at: 473 1192 1546 473 Size: 719 354 2985 Fragments arranged by size: 2985 719 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1641 1774 2233 2442 1641 Size: 133 459 209 3257 Fragments arranged by size: 3257 459 209 133 TatI w'GTAC_w Cuts at: 530 630 3573 530 Size: 100 2943 1015 Fragments arranged by size: 2943 1015 100 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 300 332 3575 3607 300 Size: 32 3243 32 751 Fragments arranged by size: 3243 751 32 32 Tth111I GACn'n_nGTC Cuts at: 1855 1855 Size: 4058 XbaI T'CTAG_A Cuts at: 1089 1089 Size: 4058 XcmI CCAnnnn_n'nnnnTGG Cuts at: 192 1553 192 Size: 1361 2697 XmaI C'CCGG_G Cuts at: 1078 1078 Size: 4058 XmnI GAAnn'nnTTC Cuts at: 375 375 Size: 4058 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI