Restriction map for
pF1KE1246
(Circular) (Six-base) MAPSORT of: pF1KE1246.seq Check: 4429 from: 1 to: 4559
pF1KE1246 4559 bp
With 182 enzymes: SgfI *
September 29, 2011 13:09 ..
AccI GT'mk_AC
Cuts at: 1527 1597 1527
Size: 70 4489
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 2384 2816 3563 3831 470
Size: 1914 432 747 268 1198
Fragments arranged by size:
1914 1198 747 432 268
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AhdI GACnn_n'nnGTC
Cuts at: 1413 1413
Size: 4559
AleI CACnn'nnGTG
Cuts at: 562 1087 562
Size: 525 4034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 870 1864 3431 870
Size: 994 1567 1998
Fragments arranged by size:
1998 1567 994
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 473 1563 3912 4168 473
Size: 1090 2349 256 864
Fragments arranged by size:
2349 1090 864 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 785 1351 1579 785
Size: 566 228 3765
Fragments arranged by size:
3765 566 228
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 500 682 1575 1989 2237 2272 500
Size: 182 893 414 248 35 2787
Fragments arranged by size:
2787 893 414 248 182 35
BanII G_rGCy'C
Cuts at: 116 169 1573 2603 116
Size: 53 1404 1030 2072
Fragments arranged by size:
2072 1404 1030 53
BbeI G_GCGC'C
Cuts at: 504 1993 2241 504
Size: 1489 248 2822
Fragments arranged by size:
2822 1489 248
BbsI GAAGACnn'nnnn_
Cuts at: 242 1280 1473 242
Size: 1038 193 3328
Fragments arranged by size:
3328 1038 193
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 773 1340 2079 773
Size: 567 739 3253
Fragments arranged by size:
3253 739 567
BglII A'GATC_T
Cuts at: 354 494 2074 4111 354
Size: 140 1580 2037 802
Fragments arranged by size:
2037 1580 802 140
BlpI GC'TnA_GC
Cuts at: 147 1671 147
Size: 1524 3035
Bme1580I G_kGCm'C
Cuts at: 685 1058 2184 2277 3333 685
Size: 373 1126 93 1056 1911
Fragments arranged by size:
1911 1126 1056 373 93
BmgBI CAC'GTC
Cuts at: 1043 1043
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 1182 1771 1852 2187 4331 1182
Size: 589 81 335 2144 1410
Fragments arranged by size:
2144 1410 589 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 742 742
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 2614 3106 3404 1735
Size: 879 492 298 2890
Fragments arranged by size:
2890 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 172 1387 4263 172
Size: 1215 2876 468
Fragments arranged by size:
2876 1215 468
BsaAI yAC'GTr
Cuts at: 593 1912 593
Size: 1319 3240
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 501 1990 2238 2940 4436 501
Size: 1489 248 702 1496 624
Fragments arranged by size:
1496 1489 702 624 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 115 1462 115
Size: 1347 3212
BseYI C'CCAG_C
Cuts at: 367 678 1150 1984 2742 3319 3815 367
Size: 311 472 834 758 577 496 1111
Fragments arranged by size:
1111 834 758 577 496 472 311
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 569 1573 2351 2541 3333 569
Size: 1004 778 190 792 1795
Fragments arranged by size:
1795 1004 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 116 169 569 685 1058 1573 2184 2277 2351
Size: 53 400 116 373 515 611 93 74
Cuts at: 2351 2541 2603 3333 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 730 611 515 400 373 190 116
93 74 62 53
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 953 1513 1609 1823 2125 2506 953
Size: 560 96 214 302 381 3006
Fragments arranged by size:
3006 560 381 302 214 96
BsrBI CCG'CTC
Cuts at: 50 761 1145 2851 2905 4389 4554 50
Size: 711 384 1706 54 1484 165 55
Fragments arranged by size:
1706 1484 711 384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 424 1097 1457 2471 424
Size: 673 360 1014 2512
Fragments arranged by size:
2512 1014 673 360
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 870 1936 870
Size: 1066 3493
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstEII G'GTnAC_C
Cuts at: 258 258
Size: 4559
BstYI r'GATC_y
Cuts at: 1 354 494 1584 2051 2074 2409 2655 3667
Size: 353 140 1090 467 23 335 246 1012
Cuts at: 3667 3753 3765 4111 1
Size: 86 12 346 449
Fragments arranged by size:
1090 1012 467 449 353 346 335 246
140 86 23 12
BtgI C'CryG_G
Cuts at: 107 263 1085 1319 107
Size: 156 822 234 3347
Fragments arranged by size:
3347 822 234 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1371 2509 2690 2853 1371
Size: 1138 181 163 3077
Fragments arranged by size:
3077 1138 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 204 615 1558 204
Size: 411 943 3205
Fragments arranged by size:
3205 943 411
DraIII CAC_nnn'GTG
Cuts at: 804 804
Size: 4559
DrdI GACnn_nn'nnGTC
Cuts at: 703 1401 2265 3123 703
Size: 698 864 858 2139
Fragments arranged by size:
2139 864 858 698
EaeI y'GGCC_r
Cuts at: 226 582 710 2144 2318 2709 2736 4081 226
Size: 356 128 1434 174 391 27 1345 704
Fragments arranged by size:
1434 1345 704 391 356 174 128 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 919 2582 2792 919
Size: 1663 210 2686
Fragments arranged by size:
2686 1663 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 587 2384 2816 3563 3831 470
Size: 117 1797 432 747 268 1198
Fragments arranged by size:
1797 1198 747 432 268 117
EcoO109I rG'GnC_Cy
Cuts at: 1309 1698 1309
Size: 389 4170
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
EcoRV GAT'ATC
Cuts at: 1284 1284
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 504 870 1993 2241 3263 4242 504
Size: 366 1123 248 1022 979 821
Fragments arranged by size:
1123 1022 979 821 366 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1399 1431 2401 2431 2433 2463 1399
Size: 32 970 30 2 30 3495
Fragments arranged by size:
3495 970 32 30 30 2
HincII GTy'rAC
Cuts at: 891 1528 1598 891
Size: 637 70 3852
Fragments arranged by size:
3852 637 70
KasI G'GCGC_C
Cuts at: 500 1989 2237 500
Size: 1489 248 2822
Fragments arranged by size:
2822 1489 248
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3230 3414 3230
Size: 184 4375
MscI TGG'CCA
Cuts at: 584 712 2320 584
Size: 128 1608 2823
Fragments arranged by size:
2823 1608 128
MslI CAynn'nnrTG
Cuts at: 292 562 1087 2548 2675 292
Size: 270 525 1461 127 2176
Fragments arranged by size:
2176 1461 525 270 127
MspA1I CmG'CkG
Cuts at: 151 276 371 1670 1984 2344 3357 3602 151
Size: 125 95 1299 314 360 1013 245 1108
Fragments arranged by size:
1299 1108 1013 360 314 245 125 95
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 501 1990 2238 501
Size: 1489 248 2822
Fragments arranged by size:
2822 1489 248
NcoI C'CATG_G
Cuts at: 107 1085 1319 107
Size: 978 234 3347
Fragments arranged by size:
3347 978 234
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 1077 1612 2112 3019 1077
Size: 535 500 907 2617
Fragments arranged by size:
2617 907 535 500
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PflMI CCAn_nnn'nTGG
Cuts at: 252 252
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PmlI CAC'GTG
Cuts at: 593 593
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PstI C_TGCA'G
Cuts at: 275 410 575 670 1606 2291 275
Size: 135 165 95 936 685 2543
Fragments arranged by size:
2543 936 685 165 135 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 151 276 371 1984 2344 151
Size: 125 95 1613 360 2366
Fragments arranged by size:
2366 1613 360 125 95
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1526 1596 1526
Size: 70 4489
SapI GCTCTTCn'nnn_
Cuts at: 919 2582 2792 919
Size: 1663 210 2686
Fragments arranged by size:
2686 1663 210
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 210 271 406 571 666 1602 2287 3280
Size: 177 61 135 165 95 936 685 993
Cuts at: 3280 3471 33
Size: 191 1121
Fragments arranged by size:
1121 993 936 685 191 177 165 135
95 61
SfoI GGC'GCC
Cuts at: 502 1991 2239 502
Size: 1489 248 2822
Fragments arranged by size:
2822 1489 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 787 1581 787
Size: 794 3765
SmlI C'TyrA_G
Cuts at: 1714 2629 3121 3383 1714
Size: 915 492 262 2890
Fragments arranged by size:
2890 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StyI C'CwwG_G
Cuts at: 107 1085 1319 1693 2047 107
Size: 978 234 374 354 2619
Fragments arranged by size:
2619 978 374 354 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 411 655 759 1164 1171 1332 2142 2275 2734
Size: 244 104 405 7 161 810 133 459
Cuts at: 2734 2943 411
Size: 209 2027
Fragments arranged by size:
2027 810 459 405 244 209 161 133
104 7
TatI w'GTAC_w
Cuts at: 575 1382 4074 575
Size: 807 2692 1060
Fragments arranged by size:
2692 1060 807
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 630 662 4076 4108 630
Size: 32 3414 32 1081
Fragments arranged by size:
3414 1081 32 32
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 785 1579 785
Size: 794 3765
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI