Restriction map for
pF1KE1303
(Circular) (Six-base) MAPSORT of: pF1KE1303.seq Check: 7238 from: 1 to: 4361
pF1KE1303 4361 bp
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 159 845 159
Size: 686 3675
AccI GT'mk_AC
Cuts at: 857 1399 857
Size: 542 3819
Acc65I G'GTAC_C
Cuts at: 1377 1377
Size: 4361
AclI AA'CG_TT
Cuts at: 4201 4201
Size: 4361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1259 2186 2618 3365 3633 1037
Size: 222 927 432 747 268 1765
Fragments arranged by size:
1765 927 747 432 268 222
AflIII A'CryG_T
Cuts at: 1713 2817 3794 1713
Size: 1104 977 2280
Fragments arranged by size:
2280 1104 977
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AhdI GACnn_n'nnGTC
Cuts at: 1248 1248
Size: 4361
AlwNI CAG_nnn'CTG
Cuts at: 250 853 1666 3233 250
Size: 603 813 1567 1378
Fragments arranged by size:
1567 1378 813 603
ApaLI G'TGCA_C
Cuts at: 3131 3131
Size: 4361
ApoI r'AATT_y
Cuts at: 1365 3714 3970 1365
Size: 2349 256 1756
Fragments arranged by size:
2349 1756 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 299 1042 1381 299
Size: 743 339 3279
Fragments arranged by size:
3279 743 339
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 140 1377 1791 2039 2074 140
Size: 1237 414 248 35 2427
Fragments arranged by size:
2427 1237 414 248 35
BanII G_rGCy'C
Cuts at: 584 638 1233 1375 2405 584
Size: 54 595 142 1030 2540
Fragments arranged by size:
2540 1030 595 142 54
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BbsI GAAGACnn'nnnn_
Cuts at: 1025 1247 1025
Size: 222 4139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 417 712 746 1033 1067 383
Size: 34 295 34 287 34 3677
Fragments arranged by size:
3677 295 287 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 281 1881 281
Size: 1600 2761
BglII A'GATC_T
Cuts at: 288 1876 3913 288
Size: 1588 2037 736
Fragments arranged by size:
2037 1588 736
BlpI GC'TnA_GC
Cuts at: 196 1473 196
Size: 1277 3084
Bme1580I G_kGCm'C
Cuts at: 1986 2079 3135 1986
Size: 93 1056 3212
Fragments arranged by size:
3212 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 424 1115 1573 1654 1989 4133 424
Size: 691 458 81 335 2144 652
Fragments arranged by size:
2144 691 652 458 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 329 1020 2728 329
Size: 691 1708 1962
Fragments arranged by size:
1962 1708 691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 527 778 1188 313
Size: 214 251 410 3486
Fragments arranged by size:
3486 410 251 214
Bpu10I CC'TnA_GC
Cuts at: 571 1261 571
Size: 690 3671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1537 2416 2908 3206 553
Size: 984 879 492 298 1708
Fragments arranged by size:
1708 984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1195 4065 1195
Size: 2870 1491
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 188 681 1097 1792 2040 2742 4238 188
Size: 493 416 695 248 702 1496 311
Fragments arranged by size:
1496 702 695 493 416 311 248
BsaWI w'CCGG_w
Cuts at: 474 1328 1550 2071 2749 3023 3170 3581 474
Size: 854 222 521 678 274 147 411 1254
Fragments arranged by size:
1254 854 678 521 411 274 222 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 681 681
Size: 4361
BseYI C'CCAG_C
Cuts at: 925 1786 2544 3121 3617 925
Size: 861 758 577 496 1669
Fragments arranged by size:
1669 861 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 887 1903 1949 3157 104
Size: 783 1016 46 1208 1308
Fragments arranged by size:
1308 1208 1016 783 46
BsiHKAI G_wGCw'C
Cuts at: 440 531 786 1375 2153 2343 3135 440
Size: 91 255 589 778 190 792 1666
Fragments arranged by size:
1666 792 778 589 255 190 91
Bsp1286I G_dGCh'C
Cuts at: 440 531 584 638 786 1233 1375 1986 2079
Size: 91 53 54 148 447 142 611 93
Cuts at: 2079 2153 2343 2405 3135 440
Size: 74 190 62 730 1666
Fragments arranged by size:
1666 730 611 447 190 148 142 93
91 74 62 54 53
BspEI T'CCGG_A
Cuts at: 1328 3581 1328
Size: 2253 2108
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 845 1253 1411 1625 1927 2308 159
Size: 686 408 158 214 302 381 2212
Fragments arranged by size:
2212 686 408 381 302 214 158
BsrBI CCG'CTC
Cuts at: 50 2653 2707 4191 4356 50
Size: 2603 54 1484 165 55
Fragments arranged by size:
2603 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2273 2273
Size: 4361
BsrFI r'CCGG_y
Cuts at: 143 2359 2540 2749 143
Size: 2216 181 209 1755
Fragments arranged by size:
2216 1755 209 181
BssSI C'ACGA_G
Cuts at: 2632 2990 2632
Size: 358 4003
BstAPI GCAn_nnn'nTGC
Cuts at: 1738 1738
Size: 4361
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 492 741 967 492
Size: 249 226 3886
Fragments arranged by size:
3886 249 226
BstYI r'GATC_y
Cuts at: 1 253 288 1386 1853 1876 2211 2457 3469
Size: 252 35 1098 467 23 335 246 1012
Cuts at: 3469 3555 3567 3913 1
Size: 86 12 346 449
Fragments arranged by size:
1098 1012 467 449 346 335 252 246
86 35 23 12
BtgI C'CryG_G
Cuts at: 107 777 987 107
Size: 670 210 3481
Fragments arranged by size:
3481 670 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2311 2492 2655 2311
Size: 181 163 4017
Fragments arranged by size:
4017 181 163
BtsI GCAGTG_nn'
Cuts at: 591 1231 1604 591
Size: 640 373 3348
Fragments arranged by size:
3348 640 373
DraI TTT'AAA
Cuts at: 1347 1360 1347
Size: 13 4348
DraIII CAC_nnn'GTG
Cuts at: 1123 1123
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 588 1946 2120 2511 2538 3883 588
Size: 1358 174 391 27 1345 1066
Fragments arranged by size:
1358 1345 1066 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 155 320 2384 2594 155
Size: 165 2064 210 1922
Fragments arranged by size:
2064 1922 210 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 527 778 1037 1188 1259 2186 2618 3365
Size: 214 251 259 151 71 927 432 747
Cuts at: 3365 3633 313
Size: 268 1041
Fragments arranged by size:
1041 927 747 432 268 259 251 214
151 71
EcoO109I rG'GnC_Cy
Cuts at: 215 1079 1173 1500 215
Size: 864 94 327 3076
Fragments arranged by size:
3076 864 327 94
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 2142 2142
Size: 4361
HaeII r_GCGC'y
Cuts at: 1795 2043 3065 4044 1795
Size: 248 1022 979 2112
Fragments arranged by size:
2112 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 273 305 1087 1119 1234 1266 2203 2233 2235
Size: 32 782 32 115 32 937 30 2
Cuts at: 2235 2265 273
Size: 30 2369
Fragments arranged by size:
2369 937 782 115 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1400 1400
Size: 4361
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 1381 1381
Size: 4361
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 680 3032 3216 680
Size: 2352 184 1825
Fragments arranged by size:
2352 1825 184
MscI TGG'CCA
Cuts at: 590 2122 590
Size: 1532 2829
MslI CAynn'nnrTG
Cuts at: 1132 2350 2477 1132
Size: 1218 127 3016
Fragments arranged by size:
3016 1218 127
MspA1I CmG'CkG
Cuts at: 240 279 920 1092 1472 1786 2146 3159 3404
Size: 39 641 172 380 314 360 1013 245
Cuts at: 3404 240
Size: 1197
Fragments arranged by size:
1197 1013 641 380 360 314 245 172
39
NaeI GCC'GGC
Cuts at: 145 2542 145
Size: 2397 1964
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 107 987 107
Size: 880 3481
NdeI CA'TA_TG
Cuts at: 221 221
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 143 2540 143
Size: 2397 1964
NspI r_CATG'y
Cuts at: 360 1414 1914 2821 360
Size: 1054 500 907 1900
Fragments arranged by size:
1900 1054 907 500
PasI CC'CwG_GG
Cuts at: 847 847
Size: 4361
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PfoI T'CCnGG_A
Cuts at: 1064 1165 1064
Size: 101 4260
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 4329
PpuMI rG'GwC_Cy
Cuts at: 1079 1173 1079
Size: 94 4267
PsiI TTA'TAA
Cuts at: 1298 1298
Size: 4361
PspXI vC'TCGA_Gb
Cuts at: 299 299
Size: 4361
PstI C_TGCA'G
Cuts at: 170 842 1250 1408 2093 170
Size: 672 408 158 685 2438
Fragments arranged by size:
2438 685 672 408 158
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 279 920 1092 1786 2146 279
Size: 641 172 694 360 2494
Fragments arranged by size:
2494 694 641 360 172
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SanDI GG'GwC_CC
Cuts at: 1173 1173
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 155 320 2384 2594 155
Size: 165 2064 210 1922
Fragments arranged by size:
2064 1922 210 165
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SfcI C'TryA_G
Cuts at: 33 166 838 1246 1404 2089 3082 3273 33
Size: 133 672 408 158 685 993 191 1121
Fragments arranged by size:
1121 993 685 672 408 191 158 133
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 299 532 1516 2431 2923 3185 299
Size: 233 984 915 492 262 1475
Fragments arranged by size:
1475 984 915 492 262 233
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StuI AGG'CCT
Cuts at: 964 964
Size: 4361
StyI C'CwwG_G
Cuts at: 107 987 1495 1849 107
Size: 880 508 354 2619
Fragments arranged by size:
2619 880 508 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 498 873 1134 1944 2077 2536 2745 498
Size: 375 261 810 133 459 209 2114
Fragments arranged by size:
2114 810 459 375 261 209 133
TatI w'GTAC_w
Cuts at: 3876 3876
Size: 4361
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 863 895 3878 3910 863
Size: 32 2983 32 1314
Fragments arranged by size:
2983 1314 32 32
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 967 1856 967
Size: 889 3472
XhoI C'TCGA_G
Cuts at: 299 299
Size: 4361
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
XmnI GAAnn'nnTTC
Cuts at: 904 904
Size: 4361
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI