Restriction map for
pF1KE1305
(Circular) (Six-base) MAPSORT of: pF1KE1305.seq Check: 1127 from: 1 to: 7193
pF1KE1305 7193 bp
With 182 enzymes: SgfI *
March 1, 2013 10:20 ..
AccI GT'mk_AC
Cuts at: 3529 4231 3529
Size: 702 6491
Acc65I G'GTAC_C
Cuts at: 4209 4209
Size: 7193
AclI AA'CG_TT
Cuts at: 7033 7033
Size: 7193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 2081 2390 2623 2822 3191 3614 4169 5018
Size: 139 309 233 199 369 423 555 849
Cuts at: 5018 5450 6197 6465 1942
Size: 432 747 268 2670
Fragments arranged by size:
2670 849 747 555 432 423 369 309
268 233 199 139
AflII C'TTAA_G
Cuts at: 1225 2767 1225
Size: 1542 5651
AflIII A'CryG_T
Cuts at: 4545 5649 6626 4545
Size: 1104 977 5112
Fragments arranged by size:
5112 1104 977
AgeI A'CCGG_T
Cuts at: 5581 5581
Size: 7193
AhdI GACnn_n'nnGTC
Cuts at: 3249 3249
Size: 7193
AleI CACnn'nnGTG
Cuts at: 1163 1163
Size: 7193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3727 3727
Size: 7193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 7161
AlwNI CAG_nnn'CTG
Cuts at: 868 2104 4498 6065 868
Size: 1236 2394 1567 1996
Fragments arranged by size:
2394 1996 1567 1236
ApaI G_GGCC'C
Cuts at: 278 1479 278
Size: 1201 5992
ApaLI G'TGCA_C
Cuts at: 5963 5963
Size: 7193
ApoI r'AATT_y
Cuts at: 898 1140 1399 2579 3047 3057 3970 4072 4197
Size: 242 259 1180 468 10 913 102 125
Cuts at: 4197 6546 6802 898
Size: 2349 256 1289
Fragments arranged by size:
2349 1289 1180 913 468 259 256 242
125 102 10
AseI AT'TA_AT
Cuts at: 20 2471 20
Size: 2451 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
AvaI C'yCGr_G
Cuts at: 220 4213 220
Size: 3993 3200
AvrII C'CTAG_G
Cuts at: 3897 3897
Size: 7193
BamHI G'GATC_C
Cuts at: 4218 4218
Size: 7193
BanI G'GyrC_C
Cuts at: 3640 4209 4623 4871 4906 3640
Size: 569 414 248 35 5927
Fragments arranged by size:
5927 569 414 248 35
BanII G_rGCy'C
Cuts at: 278 704 1479 4207 5237 278
Size: 426 775 2728 1030 2234
Fragments arranged by size:
2728 2234 1030 775 426
BbeI G_GCGC'C
Cuts at: 4627 4875 4627
Size: 248 6945
BbsI GAAGACnn'nnnn_
Cuts at: 2163 2501 3415 3941 2163
Size: 338 914 526 5415
Fragments arranged by size:
5415 914 526 338
BbvCI CC'TCA_GC
Cuts at: 1068 1068
Size: 7193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1264 1298 1264
Size: 34 7159
BciVI GTATCCnnnnn_n'
Cuts at: 2838 5091 5852 7193 2838
Size: 2253 761 1341 2838
Fragments arranged by size:
2838 2253 1341 761
BclI T'GATC_A
Cuts at: 1266 2196 4713 1266
Size: 930 2517 3746
Fragments arranged by size:
3746 2517 930
BfrBI ATG'CAT
Cuts at: 1285 1996 3714 3914 1285
Size: 711 1718 200 4564
Fragments arranged by size:
4564 1718 711 200
BglI GCCn_nnn'nGGC
Cuts at: 172 223 172
Size: 51 7142
BglII A'GATC_T
Cuts at: 1643 2269 2556 4708 6745 1643
Size: 626 287 2152 2037 2091
Fragments arranged by size:
2152 2091 2037 626 287
BlpI GC'TnA_GC
Cuts at: 4305 4305
Size: 7193
Bme1580I G_kGCm'C
Cuts at: 278 1479 2448 4818 4911 5967 278
Size: 1201 969 2370 93 1056 1504
Fragments arranged by size:
2370 1504 1201 1056 969 93
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1482 1810 1873 1977 3331 4405 4486 4821
Size: 26 328 63 104 1354 1074 81 335
Cuts at: 4821 6965 1456
Size: 2144 1684
Fragments arranged by size:
2144 1684 1354 1074 335 328 104 81
63 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5560 5560
Size: 7193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 1556 276
Size: 1280 5913
Bpu10I CC'TnA_GC
Cuts at: 1068 1068
Size: 7193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 2581 4369 5248 5740 6038 2041
Size: 540 1788 879 492 298 3196
Fragments arranged by size:
3196 1788 879 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2600 6897 2600
Size: 4297 2896
BsaAI yAC'GTr
Cuts at: 750 4546 750
Size: 3796 3397
BsaBI GATnn'nnATC
Cuts at: 6 4707 6744 6
Size: 4701 2037 455
Fragments arranged by size:
4701 2037 455
BsaHI Gr'CG_yC
Cuts at: 4624 4872 5574 7070 4624
Size: 248 702 1496 4747
Fragments arranged by size:
4747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4382 4903 5581 5855 6002 6413 4382
Size: 521 678 274 147 411 5162
Fragments arranged by size:
5162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 377 407 1554 1584 2506 2536 2942 2972 377
Size: 30 1147 30 922 30 406 30 4598
Fragments arranged by size:
4598 1147 922 406 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1272 1650 3481 1272
Size: 378 1831 4984
Fragments arranged by size:
4984 1831 378
BseYI C'CCAG_C
Cuts at: 265 530 3940 4618 5376 5953 6449 265
Size: 265 3410 678 758 577 496 1009
Fragments arranged by size:
3410 1009 758 678 577 496 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1549 3621 662
Size: 887 2072 4234
Fragments arranged by size:
4234 2072 887
BsiEI CG_ry'CG
Cuts at: 104 204 4735 4781 5989 104
Size: 100 4531 46 1208 1308
Fragments arranged by size:
4531 1308 1208 100 46
BsiHKAI G_wGCw'C
Cuts at: 124 1253 4207 4985 5175 5967 124
Size: 1129 2954 778 190 792 1350
Fragments arranged by size:
2954 1350 1129 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 124 278 704 1253 1479 2448 4207 4818 4911
Size: 154 426 549 226 969 1759 611 93
Cuts at: 4911 4985 5175 5237 5967 124
Size: 74 190 62 730 1350
Fragments arranged by size:
1759 1350 969 730 611 549 426 226
190 154 93 74 62
BspEI T'CCGG_A
Cuts at: 6413 6413
Size: 7193
BspHI T'CATG_A
Cuts at: 1206 1460 6369 1206
Size: 254 4909 2030
Fragments arranged by size:
4909 2030 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2763 4243 4457 4759 5140 2763
Size: 1480 214 302 381 4816
Fragments arranged by size:
4816 1480 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5485 5539 7023 7188 50
Size: 5435 54 1484 165 55
Fragments arranged by size:
5435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 638 2440 5105 638
Size: 1802 2665 2726
Fragments arranged by size:
2726 2665 1802
BsrFI r'CCGG_y
Cuts at: 5191 5372 5581 5191
Size: 181 209 6803
Fragments arranged by size:
6803 209 181
BsrGI T'GTAC_A
Cuts at: 128 498 128
Size: 370 6823
BssSI C'ACGA_G
Cuts at: 1550 1766 5464 5822 1550
Size: 216 3698 358 2921
Fragments arranged by size:
3698 2921 358 216
BstAPI GCAn_nnn'nTGC
Cuts at: 4021 4570 4021
Size: 549 6644
BstBI TT'CG_AA
Cuts at: 5554 5554
Size: 7193
BstEII G'GTnAC_C
Cuts at: 412 412
Size: 7193
BstXI CCAn_nnnn'nTGG
Cuts at: 285 1165 285
Size: 880 6313
BstYI r'GATC_y
Cuts at: 1 817 1643 2269 2556 4218 4685 4708 5043
Size: 816 826 626 287 1662 467 23 335
Cuts at: 5043 5289 6301 6387 6399 6745 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1662 1012 826 816 626 467 449 346
335 287 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1304 1304
Size: 7193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5143 5324 5487 5143
Size: 181 163 6849
Fragments arranged by size:
6849 181 163
BtsI GCAGTG_nn'
Cuts at: 936 951 2324 3278 4436 936
Size: 15 1373 954 1158 3693
Fragments arranged by size:
3693 1373 1158 954 15
DraI TTT'AAA
Cuts at: 551 4192 551
Size: 3641 3552
DrdI GACnn_nn'nnGTC
Cuts at: 2100 4899 5757 2100
Size: 2799 858 3536
Fragments arranged by size:
3536 2799 858
EaeI y'GGCC_r
Cuts at: 179 201 1506 1758 4778 4952 5343 5370 6715
Size: 22 1305 252 3020 174 391 27 1345
Cuts at: 6715 179
Size: 657
Fragments arranged by size:
3020 1345 1305 657 391 252 174 27
22
EagI C'GGCC_G
Cuts at: 201 4778 201
Size: 4577 2616
EarI CTCTTCn'nnn_
Cuts at: 710 719 785 1003 1236 1961 2599 3458 3750
Size: 9 66 218 233 725 638 859 292
Cuts at: 3750 5216 5426 710
Size: 1466 210 2477
Fragments arranged by size:
2477 1466 859 725 638 292 233 218
210 66 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5401 5711 5857 7003 5401
Size: 310 146 1146 5591
Fragments arranged by size:
5591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4205 4205
Size: 7193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 1556 1942 2081 2390 2623 2822 3191 3614
Size: 1280 386 139 309 233 199 369 423
Cuts at: 3614 4169 5018 5450 6197 6465 276
Size: 555 849 432 747 268 1004
Fragments arranged by size:
1280 1004 849 747 555 432 423 386
369 309 268 233 199 139
EcoNI CCTnn'n_nnAGG
Cuts at: 180 236 2770 180
Size: 56 2534 4603
Fragments arranged by size:
4603 2534 56
EcoO109I rG'GnC_Cy
Cuts at: 185 224 281 4332 185
Size: 39 57 4051 3046
Fragments arranged by size:
4051 3046 57 39
EcoRI G'AATT_C
Cuts at: 4197 4197
Size: 7193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5212 5212
Size: 7193
FspI TGC'GCA
Cuts at: 4974 4974
Size: 7193
HaeII r_GCGC'y
Cuts at: 157 352 4627 4875 5897 6876 157
Size: 195 4275 248 1022 979 474
Fragments arranged by size:
4275 1022 979 474 248 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1251 1283 1344 1376 1554 1586 2194
Size: 32 531 32 61 32 178 32 608
Cuts at: 2194 2226 2506 2538 2781 2813 3082 3114 3235
Size: 32 280 32 243 32 269 32 121
Cuts at: 3235 3267 5035 5065 5067 5097 688
Size: 32 1768 30 2 30 2784
Fragments arranged by size:
2784 1768 608 531 280 269 243 178
121 61 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2120 4136 4232 2120
Size: 2016 96 5081
Fragments arranged by size:
5081 2016 96
HindIII A'AGCT_T
Cuts at: 2548 2548
Size: 7193
KasI G'GCGC_C
Cuts at: 4623 4871 4623
Size: 248 6945
KpnI G_GTAC'C
Cuts at: 4213 4213
Size: 7193
MfeI C'AATT_G
Cuts at: 3614 3614
Size: 7193
MluI A'CGCG_T
Cuts at: 6626 6626
Size: 7193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3257 5864 6048 3257
Size: 2607 184 4402
Fragments arranged by size:
4402 2607 184
MscI TGG'CCA
Cuts at: 1760 4954 1760
Size: 3194 3999
MslI CAynn'nnrTG
Cuts at: 1163 1421 1766 2062 2506 3709 5182 5309 1163
Size: 258 345 296 444 1203 1473 127 3047
Fragments arranged by size:
3047 1473 1203 444 345 296 258 127
MspA1I CmG'CkG
Cuts at: 214 3042 4304 4618 4978 5991 6236 214
Size: 2828 1262 314 360 1013 245 1171
Fragments arranged by size:
2828 1262 1171 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5374 5374
Size: 7193
NarI GG'CG_CC
Cuts at: 4624 4872 4624
Size: 248 6945
NdeI CA'TA_TG
Cuts at: 1773 3905 1773
Size: 2132 5061
NgoMIV G'CCGG_C
Cuts at: 5372 5372
Size: 7193
NruI TCG'CGA
Cuts at: 114 114
Size: 7193
NsiI A_TGCA'T
Cuts at: 1287 1998 3716 3916 1287
Size: 711 1718 200 4564
Fragments arranged by size:
4564 1718 711 200
NspI r_CATG'y
Cuts at: 3207 3718 3914 4246 4746 5653 3207
Size: 511 196 332 500 907 4747
Fragments arranged by size:
4747 907 511 500 332 196
PciI A'CATG_T
Cuts at: 5649 5649
Size: 7193
PfoI T'CCnGG_A
Cuts at: 2960 2960
Size: 7193
PmeI GTTT'AAAC
Cuts at: 4192 4192
Size: 7193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 6332 6364 2942
Size: 32 3358 32 3771
Fragments arranged by size:
3771 3358 32 32
PshAI GACnn'nnGTC
Cuts at: 1184 3007 1184
Size: 1823 5370
PspOMI G'GGCC_C
Cuts at: 274 1475 274
Size: 1201 5992
PstI C_TGCA'G
Cuts at: 2789 4240 4925 2789
Size: 1451 685 5057
Fragments arranged by size:
5057 1451 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7193
PvuII CAG'CTG
Cuts at: 3042 4618 4978 3042
Size: 1576 360 5257
Fragments arranged by size:
5257 1576 360
RsrII CG'GwC_CG
Cuts at: 5388 5388
Size: 7193
SacI G_AGCT'C
Cuts at: 4207 4207
Size: 7193
SalI G'TCGA_C
Cuts at: 4230 4230
Size: 7193
SapI GCTCTTCn'nnn_
Cuts at: 5216 5426 5216
Size: 210 6983
SbfI CC_TGCA'GG
Cuts at: 4240 4240
Size: 7193
ScaI AGT'ACT
Cuts at: 6710 6710
Size: 7193
SfcI C'TryA_G
Cuts at: 33 1647 1710 2785 2825 2997 3257 3303 4139
Size: 1614 63 1075 40 172 260 46 836
Cuts at: 4139 4236 4921 5914 6105 33
Size: 97 685 993 191 1121
Fragments arranged by size:
1614 1121 1075 993 836 685 260 191
172 97 63 46 40
SfoI GGC'GCC
Cuts at: 4625 4873 4625
Size: 248 6945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
SmaI CCC'GGG
Cuts at: 222 4215 222
Size: 3993 3200
SmlI C'TyrA_G
Cuts at: 1225 2020 2560 2767 4348 5263 5755 6017 1225
Size: 795 540 207 1581 915 492 262 2401
Fragments arranged by size:
2401 1581 915 795 540 492 262 207
SnaBI TAC'GTA
Cuts at: 750 750
Size: 7193
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7193
SphI G_CATG'C
Cuts at: 3207 3718 3914 4246 4746 3207
Size: 511 196 332 500 5654
Fragments arranged by size:
5654 511 500 332 196
SspI AAT'ATT
Cuts at: 2032 2032
Size: 7193
StuI AGG'CCT
Cuts at: 175 974 1263 175
Size: 799 289 6105
Fragments arranged by size:
6105 799 289
StyI C'CwwG_G
Cuts at: 284 308 1661 2240 3897 4327 4681 284
Size: 24 1353 579 1657 430 354 2796
Fragments arranged by size:
2796 1657 1353 579 430 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2633 4776 4909 5368 5577 2633
Size: 2143 133 459 209 4249
Fragments arranged by size:
4249 2143 459 209 133
TatI w'GTAC_w
Cuts at: 128 498 798 1324 1881 3670 6708 128
Size: 370 300 526 557 1789 3038 613
Fragments arranged by size:
3038 1789 613 557 526 370 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6710 6742 6710
Size: 32 7161
Tth111I GACn'n_nGTC
Cuts at: 2254 4990 2254
Size: 2736 4457
XbaI T'CTAG_A
Cuts at: 2481 4224 2481
Size: 1743 5450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 451 527 2410 4688 451
Size: 76 1883 2278 2956
Fragments arranged by size:
2956 2278 1883 76
XmaI C'CCGG_G
Cuts at: 220 4213 220
Size: 3993 3200
XmnI GAAnn'nnTTC
Cuts at: 2234 4043 2234
Size: 1809 5384
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NruI
NsiI NspI PciI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AscI BaeI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstZ17I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI PacI
PasI PflMI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI ZraI