Restriction map for
pF1KE1327
(Circular) (Six-base) MAPSORT of: pF1KE1327.seq Check: 3723 from: 1 to: 4520
pF1KE1327 4520 bp
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1330 1369 1330
Size: 39 4481
AccI GT'mk_AC
Cuts at: 1558 1558
Size: 4520
Acc65I G'GTAC_C
Cuts at: 1064 1536 1064
Size: 472 4048
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1046 1331 2345 2777 3524 3792 598
Size: 448 285 1014 432 747 268 1326
Fragments arranged by size:
1326 1014 747 448 432 285 268
AflIII A'CryG_T
Cuts at: 822 877 1872 2976 3953 822
Size: 55 995 1104 977 1389
Fragments arranged by size:
1389 1104 995 977 55
AgeI A'CCGG_T
Cuts at: 2908 2908
Size: 4520
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 758 790 758
Size: 32 4488
AlwNI CAG_nnn'CTG
Cuts at: 492 1354 1408 1825 3392 492
Size: 862 54 417 1567 1620
Fragments arranged by size:
1620 1567 862 417 54
ApaI G_GGCC'C
Cuts at: 129 751 129
Size: 622 3898
ApaLI G'TGCA_C
Cuts at: 3290 3290
Size: 4520
ApoI r'AATT_y
Cuts at: 811 1524 3873 4129 811
Size: 713 2349 256 1202
Fragments arranged by size:
2349 1202 713 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 190 1417 1540 190
Size: 1227 123 3170
Fragments arranged by size:
3170 1227 123
BamHI G'GATC_C
Cuts at: 1151 1545 1151
Size: 394 4126
BanI G'GyrC_C
Cuts at: 1064 1536 1950 2198 2233 1064
Size: 472 414 248 35 3351
Fragments arranged by size:
3351 472 414 248 35
BanII G_rGCy'C
Cuts at: 129 474 647 751 934 1534 2564 129
Size: 345 173 104 183 600 1030 2085
Fragments arranged by size:
2085 1030 600 345 183 173 104
BbeI G_GCGC'C
Cuts at: 1954 2202 1954
Size: 248 4272
BbsI GAAGACnn'nnnn_
Cuts at: 906 906
Size: 4520
BbvCI CC'TCA_GC
Cuts at: 1426 1426
Size: 4520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1236 1270 1236
Size: 34 4486
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3179 4520 2418
Size: 761 1341 2418
Fragments arranged by size:
2418 1341 761
BclI T'GATC_A
Cuts at: 2040 2040
Size: 4520
BglI GCCn_nnn'nGGC
Cuts at: 452 1120 452
Size: 668 3852
BglII A'GATC_T
Cuts at: 617 2035 4072 617
Size: 1418 2037 1065
Fragments arranged by size:
2037 1418 1065
BlpI GC'TnA_GC
Cuts at: 1632 1632
Size: 4520
Bme1580I G_kGCm'C
Cuts at: 129 751 1190 1281 1302 2145 2238 3294 129
Size: 622 439 91 21 843 93 1056 1355
Fragments arranged by size:
1355 1056 843 622 439 93 91 21
BmrI ACTGGGnnnn_n'
Cuts at: 144 532 1732 1813 2148 4292 144
Size: 388 1200 81 335 2144 372
Fragments arranged by size:
2144 1200 388 372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 606 2887 606
Size: 2281 2239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 730 1477 458
Size: 272 747 3501
Fragments arranged by size:
3501 747 272
Bpu10I CC'TnA_GC
Cuts at: 1426 1426
Size: 4520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 1696 2575 3067 3365 1531
Size: 165 879 492 298 2686
Fragments arranged by size:
2686 879 492 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 1005 4224 1005
Size: 3219 1301
BsaAI yAC'GTr
Cuts at: 880 1395 1873 880
Size: 515 478 3527
Fragments arranged by size:
3527 515 478
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 1951 2199 2901 4397 1951
Size: 248 702 1496 2074
Fragments arranged by size:
2074 1496 702 248
BsaWI w'CCGG_w
Cuts at: 637 1709 2230 2908 3182 3329 3740 637
Size: 1072 521 678 274 147 411 1417
Fragments arranged by size:
1417 1072 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1307 1337 1307
Size: 30 4490
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 992 1331 992
Size: 339 4181
BseYI C'CCAG_C
Cuts at: 1358 1464 1473 1945 2703 3280 3776 1358
Size: 106 9 472 758 577 496 2102
Fragments arranged by size:
2102 758 577 496 472 106 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 649 1367 193
Size: 456 718 3346
Fragments arranged by size:
3346 718 456
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3316 104
Size: 1958 46 1208 1308
Fragments arranged by size:
1958 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 988 1534 2312 2502 3294 934
Size: 54 546 778 190 792 2160
Fragments arranged by size:
2160 792 778 546 190 54
Bsp1286I G_dGCh'C
Cuts at: 129 474 647 751 934 988 1190 1281 1302
Size: 345 173 104 183 54 202 91 21
Cuts at: 1302 1534 2145 2238 2312 2502 2564 3294 129
Size: 232 611 93 74 190 62 730 1355
Fragments arranged by size:
1355 730 611 345 232 202 190 183
173 104 93 91 74 62 54 21
BspEI T'CCGG_A
Cuts at: 3740 3740
Size: 4520
BspHI T'CATG_A
Cuts at: 198 1487 3696 198
Size: 1289 2209 1022
Fragments arranged by size:
2209 1289 1022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 1058 1330 1339 1369 1570 1784 2086 2467
Size: 843 272 9 30 201 214 302 381
Cuts at: 2467 215
Size: 2268
Fragments arranged by size:
2268 843 381 302 272 214 201 30
9
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1441 2432 1441
Size: 991 3529
BsrFI r'CCGG_y
Cuts at: 2518 2699 2908 2518
Size: 181 209 4130
Fragments arranged by size:
4130 209 181
BssSI C'ACGA_G
Cuts at: 310 1192 2791 3149 310
Size: 882 1599 358 1681
Fragments arranged by size:
1681 1599 882 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1248 1345 1897 1248
Size: 97 552 3871
Fragments arranged by size:
3871 552 97
BstBI TT'CG_AA
Cuts at: 2881 2881
Size: 4520
BstXI CCAn_nnnn'nTGG
Cuts at: 120 292 298 388 120
Size: 172 6 90 4252
Fragments arranged by size:
4252 172 90 6
BstYI r'GATC_y
Cuts at: 1 617 1151 1545 2012 2035 2370 2616 3628
Size: 616 534 394 467 23 335 246 1012
Cuts at: 3628 3714 3726 4072 1
Size: 86 12 346 449
Fragments arranged by size:
1012 616 534 467 449 394 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 143 234 273 291 297 348 375 387
Size: 36 91 39 18 6 51 27 12
Cuts at: 387 447 578 107
Size: 60 131 4049
Fragments arranged by size:
4049 131 91 60 51 39 36 27
18 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 586 1261 2470 2651 2814 586
Size: 675 1209 181 163 2292
Fragments arranged by size:
2292 1209 675 181 163
BtsI GCAGTG_nn'
Cuts at: 1328 1335 1763 1328
Size: 7 428 4085
Fragments arranged by size:
4085 428 7
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DraIII CAC_nnn'GTG
Cuts at: 138 138
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 194 2226 3084 194
Size: 2032 858 1630
Fragments arranged by size:
2032 1630 858
EaeI y'GGCC_r
Cuts at: 110 140 237 276 294 300 378 741 1112
Size: 30 97 39 18 6 78 363 371
Cuts at: 1112 2105 2279 2670 2697 4042 110
Size: 993 174 391 27 1345 588
Fragments arranged by size:
1345 993 588 391 371 363 174 97
78 39 30 27 18 6
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 571 964 2543 2753 571
Size: 393 1579 210 2338
Fragments arranged by size:
2338 1579 393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2728 3038 3184 4330 2728
Size: 310 146 1146 2918
Fragments arranged by size:
2918 1146 310 146
EcoICRI GAG'CTC
Cuts at: 932 1532 932
Size: 600 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 598 730 1046 1331 1477 2345 2777 3524
Size: 140 132 316 285 146 868 432 747
Cuts at: 3524 3792 458
Size: 268 1186
Fragments arranged by size:
1186 868 747 432 316 285 268 146
140 132
EcoO109I rG'GnC_Cy
Cuts at: 125 126 489 747 748 1351 1362 1659 125
Size: 1 363 258 1 603 11 297 2986
Fragments arranged by size:
2986 603 363 297 258 11 1 1
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4520
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 1954 2202 3224 4203 1954
Size: 248 1022 979 2271
Fragments arranged by size:
2271 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1136 1168 2362 2392 2394 2424 1136
Size: 32 1194 30 2 30 3232
Fragments arranged by size:
3232 1194 32 30 30 2
HincII GTy'rAC
Cuts at: 1559 1559
Size: 4520
KasI G'GCGC_C
Cuts at: 1950 2198 1950
Size: 248 4272
KpnI G_GTAC'C
Cuts at: 1068 1540 1068
Size: 472 4048
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1179 3191 3375 1179
Size: 2012 184 2324
Fragments arranged by size:
2324 2012 184
MscI TGG'CCA
Cuts at: 112 239 278 296 302 380 743 1114 2281
Size: 127 39 18 6 78 363 371 1167
Cuts at: 2281 112
Size: 2351
Fragments arranged by size:
2351 1167 371 363 127 78 39 18
6
MslI CAynn'nnrTG
Cuts at: 257 284 290 296 347 359 386 428 434
Size: 27 6 6 51 12 27 42 6
Cuts at: 434 446 848 854 878 893 1492 2509 2636
Size: 12 402 6 24 15 599 1017 127
Cuts at: 2636 257
Size: 2141
Fragments arranged by size:
2141 1017 599 402 127 51 42 27
27 24 15 12 12 6 6 6
6
MspA1I CmG'CkG
Cuts at: 1631 1945 2305 3318 3563 1631
Size: 314 360 1013 245 2588
Fragments arranged by size:
2588 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2701 2701
Size: 4520
NarI GG'CG_CC
Cuts at: 1951 2199 1951
Size: 248 4272
NcoI C'CATG_G
Cuts at: 107 234 273 291 297 348 375 387 447
Size: 127 39 18 6 51 27 12 60
Cuts at: 447 107
Size: 4180
Fragments arranged by size:
4180 127 60 51 39 27 18 12
6
NgoMIV G'CCGG_C
Cuts at: 2699 2699
Size: 4520
NspI r_CATG'y
Cuts at: 826 1573 2073 2980 826
Size: 747 500 907 2366
Fragments arranged by size:
2366 907 747 500
PciI A'CATG_T
Cuts at: 822 2976 822
Size: 2154 2366
PflMI CCAn_nnn'nTGG
Cuts at: 138 708 138
Size: 570 3950
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PmlI CAC'GTG
Cuts at: 880 880
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4488
PpuMI rG'GwC_Cy
Cuts at: 489 1351 1362 489
Size: 862 11 3647
Fragments arranged by size:
3647 862 11
PspOMI G'GGCC_C
Cuts at: 125 747 125
Size: 622 3898
PstI C_TGCA'G
Cuts at: 1234 1380 1567 2252 1234
Size: 146 187 685 3502
Fragments arranged by size:
3502 685 187 146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 1945 2305 1945
Size: 360 4160
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 934 1534 934
Size: 600 3920
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 2543 2753 2543
Size: 210 4310
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SfcI C'TryA_G
Cuts at: 33 1230 1376 1563 2248 3241 3432 33
Size: 1197 146 187 685 993 191 1121
Fragments arranged by size:
1197 1121 993 685 191 187 146
SfoI GGC'GCC
Cuts at: 1952 2200 1952
Size: 248 4272
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 1510 1675 2590 3082 3344 1510
Size: 165 915 492 262 2686
Fragments arranged by size:
2686 915 492 262 165
SnaBI TAC'GTA
Cuts at: 1395 1395
Size: 4520
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1573 2073 1573
Size: 500 4020
StuI AGG'CCT
Cuts at: 119 119
Size: 4520
StyI C'CwwG_G
Cuts at: 107 167 234 273 291 297 348 375 387
Size: 60 67 39 18 6 51 27 12
Cuts at: 387 447 917 1654 2008 107
Size: 60 470 737 354 2619
Fragments arranged by size:
2619 737 470 354 67 60 60 51
39 27 18 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 2103 2236 2695 2904 126
Size: 1977 133 459 209 1742
Fragments arranged by size:
1977 1742 459 209 133
TatI w'GTAC_w
Cuts at: 4035 4035
Size: 4520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1307 1339 4037 4069 1307
Size: 32 2698 32 1758
Fragments arranged by size:
2698 1758 32 32
Tth111I GACn'n_nGTC
Cuts at: 839 2317 839
Size: 1478 3042
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 421 454 759 2015 421
Size: 33 305 1256 2926
Fragments arranged by size:
2926 1256 305 33
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI