Restriction map for
pF1KE1328
(Circular) (Six-base) MAPSORT of: pF1KE1328.seq Check: 6338 from: 1 to: 4205
pF1KE1328 4205 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 679 679
Size: 4205
AccI GT'mk_AC
Cuts at: 1243 1243
Size: 4205
Acc65I G'GTAC_C
Cuts at: 752 1221 752
Size: 469 3736
AclI AA'CG_TT
Cuts at: 4045 4045
Size: 4205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 721 2030 2462 3209 3477 250
Size: 471 1309 432 747 268 978
Fragments arranged by size:
1309 978 747 471 432 268
AflIII A'CryG_T
Cuts at: 176 178 1557 2661 3638 176
Size: 2 1379 1104 977 743
Fragments arranged by size:
1379 1104 977 743 2
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 661 661
Size: 4205
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaLI G'TGCA_C
Cuts at: 636 2975 636
Size: 2339 1866
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 529 1225 529
Size: 696 3509
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 307 752 1221 1635 1883 1918 307
Size: 445 469 414 248 35 2594
Fragments arranged by size:
2594 469 445 414 248 35
BanII G_rGCy'C
Cuts at: 809 1132 1219 2249 809
Size: 323 87 1030 2765
Fragments arranged by size:
2765 1030 323 87
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbsI GAAGACnn'nnnn_
Cuts at: 164 339 164
Size: 175 4030
BbvCI CC'TCA_GC
Cuts at: 1076 1076
Size: 4205
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 502 1317 502
Size: 815 3390
Bme1580I G_kGCm'C
Cuts at: 310 640 1830 1923 2979 310
Size: 330 1190 93 1056 1536
Fragments arranged by size:
1536 1190 1056 330 93
BmrI ACTGGGnnnn_n'
Cuts at: 1117 1417 1498 1833 3977 1117
Size: 300 81 335 2144 1345
Fragments arranged by size:
2144 1345 335 300 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 823 508
Size: 315 3890
Bpu10I CC'TnA_GC
Cuts at: 1076 1076
Size: 4205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1381 2260 2752 3050 706
Size: 675 879 492 298 1861
Fragments arranged by size:
1861 879 675 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1034 1143 3909 1034
Size: 109 2766 1330
Fragments arranged by size:
2766 1330 109
BsaAI yAC'GTr
Cuts at: 179 1558 179
Size: 1379 2826
BsaBI GATnn'nnATC
Cuts at: 6 1719 3756 6
Size: 1713 2037 455
Fragments arranged by size:
2037 1713 455
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2586 4082 1636
Size: 248 702 1496 1759
Fragments arranged by size:
1759 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1394 1915 2593 2867 3014 3425 1394
Size: 521 678 274 147 411 2174
Fragments arranged by size:
2174 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 285 646 285
Size: 361 3844
BseYI C'CCAG_C
Cuts at: 129 482 1630 2388 2965 3461 129
Size: 353 1148 758 577 496 873
Fragments arranged by size:
1148 873 758 577 496 353
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3001 104
Size: 1643 46 1208 1308
Fragments arranged by size:
1643 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 640 809 1132 1219 1997 2187 2979 640
Size: 169 323 87 778 190 792 1866
Fragments arranged by size:
1866 792 778 323 190 169 87
Bsp1286I G_dGCh'C
Cuts at: 310 640 809 1132 1219 1830 1923 1997 2187
Size: 330 169 323 87 611 93 74 190
Cuts at: 2187 2249 2979 310
Size: 62 730 1536
Fragments arranged by size:
1536 730 611 330 323 190 169 93
87 74 62
BspEI T'CCGG_A
Cuts at: 3425 3425
Size: 4205
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 653 679 1255 1469 1771 2152 653
Size: 26 576 214 302 381 2706
Fragments arranged by size:
2706 576 381 302 214 26
BsrBI CCG'CTC
Cuts at: 50 2497 2551 4035 4200 50
Size: 2447 54 1484 165 55
Fragments arranged by size:
2447 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 2203 2384 2593 2203
Size: 181 209 3815
Fragments arranged by size:
3815 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 141 1052 141
Size: 911 3294
BstYI r'GATC_y
Cuts at: 1 114 1230 1697 1720 2055 2301 3313 3399
Size: 113 1116 467 23 335 246 1012 86
Cuts at: 3399 3411 3757 1
Size: 12 346 449
Fragments arranged by size:
1116 1012 467 449 346 335 246 113
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 297 297
Size: 4205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1448 1448
Size: 4205
DraI TTT'AAA
Cuts at: 942 1204 942
Size: 262 3943
DrdI GACnn_nn'nnGTC
Cuts at: 343 1911 2769 343
Size: 1568 858 1779
Fragments arranged by size:
1779 1568 858
EaeI y'GGCC_r
Cuts at: 1790 1964 2355 2382 3727 1790
Size: 174 391 27 1345 2268
Fragments arranged by size:
2268 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 724 1027 2413 2723 2869 4015 724
Size: 303 1386 310 146 1146 914
Fragments arranged by size:
1386 1146 914 310 303 146
EcoICRI GAG'CTC
Cuts at: 807 1130 1217 807
Size: 323 87 3795
Fragments arranged by size:
3795 323 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 508 721 823 2030 2462 3209 3477 250
Size: 258 213 102 1207 432 747 268 978
Fragments arranged by size:
1207 978 747 432 268 258 213 102
EcoO109I rG'GnC_Cy
Cuts at: 1118 1344 1118
Size: 226 3979
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 711 1639 1887 2909 3888 711
Size: 928 248 1022 979 1028
Fragments arranged by size:
1028 1022 979 928 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 386 418 2047 2077 2079 2109 386
Size: 32 1629 30 2 30 2482
Fragments arranged by size:
2482 1629 32 30 30 2
HincII GTy'rAC
Cuts at: 584 1244 584
Size: 660 3545
HindIII A'AGCT_T
Cuts at: 772 772
Size: 4205
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 756 1225 756
Size: 469 3736
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1033 2876 3060 1033
Size: 1843 184 2178
Fragments arranged by size:
2178 1843 184
MscI TGG'CCA
Cuts at: 1966 1966
Size: 4205
MslI CAynn'nnrTG
Cuts at: 2194 2321 2194
Size: 127 4078
MspA1I CmG'CkG
Cuts at: 423 1062 1316 1630 1990 3003 3248 423
Size: 639 254 314 360 1013 245 1380
Fragments arranged by size:
1380 1013 639 360 314 254 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 192 1258 1758 2665 192
Size: 1066 500 907 1732
Fragments arranged by size:
1732 1066 907 500
PasI CC'CwG_GG
Cuts at: 573 864 573
Size: 291 3914
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PfoI T'CCnGG_A
Cuts at: 404 1001 1132 404
Size: 597 131 3477
Fragments arranged by size:
3477 597 131
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PmlI CAC'GTG
Cuts at: 179 179
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 678 3344 3376 646
Size: 32 2666 32 1475
Fragments arranged by size:
2666 1475 32 32
PpuMI rG'GwC_Cy
Cuts at: 1118 1118
Size: 4205
PstI C_TGCA'G
Cuts at: 650 1252 1937 650
Size: 602 685 2918
Fragments arranged by size:
2918 685 602
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 423 1062 1630 1990 423
Size: 639 568 360 2638
Fragments arranged by size:
2638 639 568 360
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 809 1132 1219 809
Size: 323 87 3795
Fragments arranged by size:
3795 323 87
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SanDI GG'GwC_CC
Cuts at: 1118 1118
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 2228 2438 2228
Size: 210 3995
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 4205
SfcI C'TryA_G
Cuts at: 33 646 685 1019 1248 1933 2926 3117 33
Size: 613 39 334 229 685 993 191 1121
Fragments arranged by size:
1121 993 685 613 334 229 191 39
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 1227 1227
Size: 4205
SmlI C'TyrA_G
Cuts at: 721 1360 2275 2767 3029 721
Size: 639 915 492 262 1897
Fragments arranged by size:
1897 915 639 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 192 1258 1758 192
Size: 1066 500 2639
Fragments arranged by size:
2639 1066 500
StuI AGG'CCT
Cuts at: 993 1081 993
Size: 88 4117
StyI C'CwwG_G
Cuts at: 275 1339 1693 275
Size: 1064 354 2787
Fragments arranged by size:
2787 1064 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1788 1921 2380 2589 1021
Size: 767 133 459 209 2637
Fragments arranged by size:
2637 767 459 209 133
TatI w'GTAC_w
Cuts at: 3720 3720
Size: 4205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1130 1700 1130
Size: 570 3635
XmaI C'CCGG_G
Cuts at: 1225 1225
Size: 4205
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI