Restriction map for
pF1KE1345
(Circular) (Six-base) MAPSORT of: pF1KE1345.seq Check: 7016 from: 1 to: 3905
pF1KE1345 3905 bp
With 182 enzymes: SgfI *
February 23, 2015 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 507
Size: 3905
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 544 688 1730 2162 2909 3177 421
Size: 123 144 1042 432 747 268 1149
Fragments arranged by size:
1149 1042 747 432 268 144 123
AflII C'TTAA_G
Cuts at: 220 220
Size: 3905
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AhdI GACnn_n'nnGTC
Cuts at: 508 508
Size: 3905
AlwNI CAG_nnn'CTG
Cuts at: 378 387 522 552 1210 2777 378
Size: 9 135 30 658 1567 1506
Fragments arranged by size:
1567 1506 658 135 30 9
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 909 3258 3514 909
Size: 2349 256 1300
Fragments arranged by size:
2349 1300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 158 925 158
Size: 767 3138
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 921 1335 1583 1618 921
Size: 414 248 35 3208
Fragments arranged by size:
3208 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 689 1425 689
Size: 736 3169
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 589 886 1017 589
Size: 297 131 3477
Fragments arranged by size:
3477 297 131
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 427 562 1117 1198 1533 3677 427
Size: 135 555 81 335 2144 655
Fragments arranged by size:
2144 655 555 335 135 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 2272 837
Size: 1435 2470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 726 409
Size: 317 3588
Bpu10I CC'TnA_GC
Cuts at: 646 646
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1081 1960 2452 2750 688
Size: 393 879 492 298 1843
Fragments arranged by size:
1843 879 492 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 269 3609 269
Size: 3340 565
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 289 1419 3456 6
Size: 283 1130 2037 455
Fragments arranged by size:
2037 1130 455 283
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 1094 1615 2293 2567 2714 3125 1094
Size: 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 481
Size: 30 3875
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 505 310
Size: 195 3710
BseYI C'CCAG_C
Cuts at: 1330 2088 2665 3161 1330
Size: 758 577 496 2074
Fragments arranged by size:
2074 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 590 919 1697 1887 2679 590
Size: 329 778 190 792 1816
Fragments arranged by size:
1816 792 778 329 190
Bsp1286I G_dGCh'C
Cuts at: 590 919 1530 1623 1697 1887 1949 2679 590
Size: 329 611 93 74 190 62 730 1816
Fragments arranged by size:
1816 730 611 329 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3125 3125
Size: 3905
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 386 507 955 1169 1471 1852 287
Size: 99 121 448 214 302 381 2340
Fragments arranged by size:
2340 448 381 302 214 121 99
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstEII G'GTnAC_C
Cuts at: 438 438
Size: 3905
BstYI r'GATC_y
Cuts at: 1 930 1397 1420 1755 2001 3013 3099 3111
Size: 929 467 23 335 246 1012 86 12
Cuts at: 3111 3457 1
Size: 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 842 107
Size: 735 3170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DraIII CAC_nnn'GTG
Cuts at: 599 599
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 845 1490 1664 2055 2082 3427 845
Size: 645 174 391 27 1345 1323
Fragments arranged by size:
1345 1323 645 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2113 2423 2569 3715 2113
Size: 310 146 1146 2303
Fragments arranged by size:
2303 1146 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 421 544 688 726 1730 2162 2909 3177
Size: 12 123 144 38 1004 432 747 268
Cuts at: 3177 409
Size: 1137
Fragments arranged by size:
1137 1004 747 432 268 144 123 38
12
EcoNI CCTnn'n_nnAGG
Cuts at: 636 636
Size: 3905
EcoO109I rG'GnC_Cy
Cuts at: 1044 1044
Size: 3905
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 851 1924 851
Size: 1073 2832
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 646 1339 1587 2609 3588 646
Size: 693 248 1022 979 963
Fragments arranged by size:
1022 979 963 693 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 494 526 1747 1777 1779 1809 280
Size: 32 182 32 1221 30 2 30 2376
Fragments arranged by size:
2376 1221 182 32 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 847 1666 847
Size: 819 3086
MslI CAynn'nnrTG
Cuts at: 1894 2021 1894
Size: 127 3778
MspA1I CmG'CkG
Cuts at: 1016 1330 1690 2703 2948 1016
Size: 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 107 842 107
Size: 735 3170
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PasI CC'CwG_GG
Cuts at: 753 753
Size: 3905
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PflMI CCAn_nnn'nTGG
Cuts at: 552 552
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 721 753 3044 3076 721
Size: 32 2291 32 1550
Fragments arranged by size:
2291 1550 32 32
PstI C_TGCA'G
Cuts at: 207 383 640 952 1637 207
Size: 176 257 312 685 2475
Fragments arranged by size:
2475 685 312 257 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 383 952 383
Size: 569 3336
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 203 379 636 948 1633 2626 2817 33
Size: 170 176 257 312 685 993 191 1121
Fragments arranged by size:
1121 993 685 312 257 191 176 170
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 220 703 1060 1975 2467 2729 220
Size: 483 357 915 492 262 1396
Fragments arranged by size:
1396 915 492 483 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 178 178
Size: 3905
StuI AGG'CCT
Cuts at: 418 418
Size: 3905
StyI C'CwwG_G
Cuts at: 107 128 842 1039 1393 107
Size: 21 714 197 354 2619
Fragments arranged by size:
2619 714 354 197 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 1400 750
Size: 650 3255
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI