Restriction map for
pF1KE1351
(Circular) (Six-base) MAPSORT of: pF1KE1351.seq Check: 9349 from: 1 to: 5024
pF1KE1351 5024 bp
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AccI GT'mk_AC
Cuts at: 2062 2062
Size: 5024
Acc65I G'GTAC_C
Cuts at: 570 2040 570
Size: 1470 3554
AclI AA'CG_TT
Cuts at: 4864 4864
Size: 5024
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1653 2849 3281 4028 4296 1027
Size: 626 1196 432 747 268 1755
Fragments arranged by size:
1755 1196 747 626 432 268
AflII C'TTAA_G
Cuts at: 966 966
Size: 5024
AflIII A'CryG_T
Cuts at: 2376 3480 4457 2376
Size: 1104 977 2943
Fragments arranged by size:
2943 1104 977
AgeI A'CCGG_T
Cuts at: 3412 3412
Size: 5024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 824 856 880 912 824
Size: 32 24 32 4936
Fragments arranged by size:
4936 32 32 24
AlwNI CAG_nnn'CTG
Cuts at: 438 1050 1188 2329 3896 438
Size: 612 138 1141 1567 1566
Fragments arranged by size:
1567 1566 1141 612 138
ApaLI G'TGCA_C
Cuts at: 3794 3794
Size: 5024
ApoI r'AATT_y
Cuts at: 441 1723 2028 4377 4633 441
Size: 1282 305 2349 256 832
Fragments arranged by size:
2349 1282 832 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5024
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5024
AvaI C'yCGr_G
Cuts at: 1501 2044 1501
Size: 543 4481
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1163 1196 1163
Size: 33 4991
BamHI G'GATC_C
Cuts at: 2049 2049
Size: 5024
BanI G'GyrC_C
Cuts at: 570 582 910 2040 2454 2702 2737 570
Size: 12 328 1130 414 248 35 2857
Fragments arranged by size:
2857 1130 414 328 248 35 12
BanII G_rGCy'C
Cuts at: 782 1423 1467 2038 3068 782
Size: 641 44 571 1030 2738
Fragments arranged by size:
2738 1030 641 571 44
BbeI G_GCGC'C
Cuts at: 2458 2706 2458
Size: 248 4776
BbsI GAAGACnn'nnnn_
Cuts at: 234 454 1028 234
Size: 220 574 4230
Fragments arranged by size:
4230 574 220
BciVI GTATCCnnnnn_n'
Cuts at: 1164 1816 2922 3683 5024 1164
Size: 652 1106 761 1341 1164
Fragments arranged by size:
1341 1164 1106 761 652
BclI T'GATC_A
Cuts at: 2544 2544
Size: 5024
BglI GCCn_nnn'nGGC
Cuts at: 112 1249 112
Size: 1137 3887
BglII A'GATC_T
Cuts at: 2539 4576 2539
Size: 2037 2987
BlpI GC'TnA_GC
Cuts at: 2136 2136
Size: 5024
Bme1580I G_kGCm'C
Cuts at: 2649 2742 3798 2649
Size: 93 1056 3875
Fragments arranged by size:
3875 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1590 2236 2317 2652 4796 1590
Size: 646 81 335 2144 1818
Fragments arranged by size:
2144 1818 646 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 957 1839 3391 957
Size: 882 1552 2590
Fragments arranged by size:
2590 1552 882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1367 1367
Size: 5024
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 2200 3079 3571 3869 865
Size: 1335 879 492 298 2020
Fragments arranged by size:
2020 1335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4728 4728
Size: 5024
BsaAI yAC'GTr
Cuts at: 1885 2377 1885
Size: 492 4532
BsaBI GATnn'nnATC
Cuts at: 6 2538 4575 6
Size: 2532 2037 455
Fragments arranged by size:
2532 2037 455
BsaHI Gr'CG_yC
Cuts at: 2455 2703 3405 4901 2455
Size: 248 702 1496 2578
Fragments arranged by size:
2578 1496 702 248
BsaWI w'CCGG_w
Cuts at: 683 2213 2734 3412 3686 3833 4244 683
Size: 1530 521 678 274 147 411 1463
Fragments arranged by size:
1530 1463 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 880 910 1809 1839 646
Size: 30 204 30 899 30 3831
Fragments arranged by size:
3831 899 204 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 652 667 868 1177 652
Size: 15 201 309 4499
Fragments arranged by size:
4499 309 201 15
BseYI C'CCAG_C
Cuts at: 1197 1466 1652 2449 3207 3784 4280 1197
Size: 269 186 797 758 577 496 1941
Fragments arranged by size:
1941 797 758 577 496 269 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 1609 1631 913
Size: 696 22 4306
Fragments arranged by size:
4306 696 22
BsiEI CG_ry'CG
Cuts at: 104 125 1257 2566 2612 3820 104
Size: 21 1132 1309 46 1208 1308
Fragments arranged by size:
1309 1308 1208 1132 46 21
BsiHKAI G_wGCw'C
Cuts at: 2038 2816 3006 3798 2038
Size: 778 190 792 3264
Fragments arranged by size:
3264 792 778 190
BsmI GAATG_Cn'
Cuts at: 621 621
Size: 5024
Bsp1286I G_dGCh'C
Cuts at: 782 1423 1467 2038 2649 2742 2816 3006 3068
Size: 641 44 571 611 93 74 190 62
Cuts at: 3068 3798 782
Size: 730 2008
Fragments arranged by size:
2008 730 641 611 571 190 93 74
62 44
BspEI T'CCGG_A
Cuts at: 683 4244 683
Size: 3561 1463
BspHI T'CATG_A
Cuts at: 4200 4200
Size: 5024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 288 2074 2288 2590 2971 288
Size: 1786 214 302 381 2341
Fragments arranged by size:
2341 1786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2000 3316 3370 4854 5019 50
Size: 1950 1316 54 1484 165 55
Fragments arranged by size:
1950 1484 1316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2936 2936
Size: 5024
BsrFI r'CCGG_y
Cuts at: 1256 1762 3022 3203 3412 1256
Size: 506 1260 181 209 2868
Fragments arranged by size:
2868 1260 506 209 181
BsrGI T'GTAC_A
Cuts at: 1547 1547
Size: 5024
BssSI C'ACGA_G
Cuts at: 3295 3653 3295
Size: 358 4666
BstAPI GCAn_nnn'nTGC
Cuts at: 2401 2401
Size: 5024
BstBI TT'CG_AA
Cuts at: 3385 3385
Size: 5024
BstYI r'GATC_y
Cuts at: 1 1686 2049 2516 2539 2874 3120 4132 4218
Size: 1685 363 467 23 335 246 1012 86
Cuts at: 4218 4230 4576 1
Size: 12 346 449
Fragments arranged by size:
1685 1012 467 449 363 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1589 1589
Size: 5024
BtgI C'CryG_G
Cuts at: 107 370 1298 107
Size: 263 928 3833
Fragments arranged by size:
3833 928 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 130 1932 2974 3155 3318 130
Size: 1802 1042 181 163 1836
Fragments arranged by size:
1836 1802 1042 181 163
BtsI GCAGTG_nn'
Cuts at: 722 2267 722
Size: 1545 3479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1280 1315 1280
Size: 35 4989
DraI TTT'AAA
Cuts at: 2023 2023
Size: 5024
DrdI GACnn_nn'nnGTC
Cuts at: 1402 2730 3588 1402
Size: 1328 858 2838
Fragments arranged by size:
2838 1328 858
EaeI y'GGCC_r
Cuts at: 206 422 509 540 2609 2783 3174 3201 4546
Size: 216 87 31 2069 174 391 27 1345
Cuts at: 4546 206
Size: 684
Fragments arranged by size:
2069 1345 684 391 216 174 87 31
27
EagI C'GGCC_G
Cuts at: 2609 2609
Size: 5024
EarI CTCTTCn'nnn_
Cuts at: 555 1154 1539 1861 3047 3257 555
Size: 599 385 322 1186 210 2322
Fragments arranged by size:
2322 1186 599 385 322 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1275 3232 3542 3688 4834 1275
Size: 1957 310 146 1146 1465
Fragments arranged by size:
1957 1465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2036 2036
Size: 5024
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1367 1653 2849 3281 4028 4296 1027
Size: 340 286 1196 432 747 268 1755
Fragments arranged by size:
1755 1196 747 432 340 286 268
EcoNI CCTnn'n_nnAGG
Cuts at: 487 487
Size: 5024
EcoO109I rG'GnC_Cy
Cuts at: 162 591 1095 1213 2163 162
Size: 429 504 118 950 3023
Fragments arranged by size:
3023 950 504 429 118
EcoRI G'AATT_C
Cuts at: 2028 2028
Size: 5024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 5024
FspI TGC'GCA
Cuts at: 2805 2805
Size: 5024
HaeII r_GCGC'y
Cuts at: 2458 2706 3728 4707 2458
Size: 248 1022 979 2775
Fragments arranged by size:
2775 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 1044 1076 1794 1809 1826 1841 2866
Size: 32 282 32 718 15 17 15 1025
Cuts at: 2866 2896 2898 2928 730
Size: 30 2 30 2826
Fragments arranged by size:
2826 1025 718 282 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 2063 2063
Size: 5024
HindIII A'AGCT_T
Cuts at: 361 361
Size: 5024
KasI G'GCGC_C
Cuts at: 2454 2702 2454
Size: 248 4776
KpnI G_GTAC'C
Cuts at: 574 2044 574
Size: 1470 3554
MluI A'CGCG_T
Cuts at: 4457 4457
Size: 5024
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 866 3695 3879 866
Size: 2829 184 2011
Fragments arranged by size:
2829 2011 184
MscI TGG'CCA
Cuts at: 208 511 542 2785 208
Size: 303 31 2243 2447
Fragments arranged by size:
2447 2243 303 31
MslI CAynn'nnrTG
Cuts at: 1903 3013 3140 1903
Size: 1110 127 3787
Fragments arranged by size:
3787 1110 127
MspA1I CmG'CkG
Cuts at: 155 2135 2449 2809 3822 4067 155
Size: 1980 314 360 1013 245 1112
Fragments arranged by size:
1980 1112 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1764 3205 1764
Size: 1441 3583
NarI GG'CG_CC
Cuts at: 2455 2703 2455
Size: 248 4776
NcoI C'CATG_G
Cuts at: 107 370 107
Size: 263 4761
NdeI CA'TA_TG
Cuts at: 1077 1077
Size: 5024
NgoMIV G'CCGG_C
Cuts at: 1762 3203 1762
Size: 1441 3583
NspI r_CATG'y
Cuts at: 237 2077 2577 3484 237
Size: 1840 500 907 1777
Fragments arranged by size:
1840 1777 907 500
PciI A'CATG_T
Cuts at: 3480 3480
Size: 5024
PflMI CCAn_nnn'nTGG
Cuts at: 560 957 560
Size: 397 4627
PmeI GTTT'AAAC
Cuts at: 2023 2023
Size: 5024
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 880 912 919 951 4163 4195 880
Size: 32 7 32 3212 32 1709
Fragments arranged by size:
3212 1709 32 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 162 591 1095 1213 162
Size: 429 504 118 3973
Fragments arranged by size:
3973 504 429 118
PshAI GACnn'nnGTC
Cuts at: 1351 1351
Size: 5024
PstI C_TGCA'G
Cuts at: 1034 2071 2756 1034
Size: 1037 685 3302
Fragments arranged by size:
3302 1037 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5024
PvuII CAG'CTG
Cuts at: 2449 2809 2449
Size: 360 4664
RsrII CG'GwC_CG
Cuts at: 3219 3219
Size: 5024
SacI G_AGCT'C
Cuts at: 2038 2038
Size: 5024
SalI G'TCGA_C
Cuts at: 2061 2061
Size: 5024
SanDI GG'GwC_CC
Cuts at: 1213 1213
Size: 5024
SapI GCTCTTCn'nnn_
Cuts at: 3047 3257 3047
Size: 210 4814
SbfI CC_TGCA'GG
Cuts at: 2071 2071
Size: 5024
ScaI AGT'ACT
Cuts at: 1748 4541 1748
Size: 2793 2231
SfcI C'TryA_G
Cuts at: 33 699 1030 1566 2067 2752 3745 3936 33
Size: 666 331 536 501 685 993 191 1121
Fragments arranged by size:
1121 993 685 666 536 501 331 191
SfoI GGC'GCC
Cuts at: 2456 2704 2456
Size: 248 4776
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5024
SmaI CCC'GGG
Cuts at: 1503 2046 1503
Size: 543 4481
SmlI C'TyrA_G
Cuts at: 880 966 2179 3094 3586 3848 880
Size: 86 1213 915 492 262 2056
Fragments arranged by size:
2056 1213 915 492 262 86
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5024
SphI G_CATG'C
Cuts at: 237 2077 2577 237
Size: 1840 500 2684
Fragments arranged by size:
2684 1840 500
StyI C'CwwG_G
Cuts at: 107 370 575 1010 1718 2158 2512 107
Size: 263 205 435 708 440 354 2619
Fragments arranged by size:
2619 708 440 435 354 263 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1163 1365 2607 2740 3199 3408 1163
Size: 202 1242 133 459 209 2779
Fragments arranged by size:
2779 1242 459 209 202 133
TatI w'GTAC_w
Cuts at: 641 1508 1547 1746 4539 641
Size: 867 39 199 2793 1126
Fragments arranged by size:
2793 1126 867 199 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 710 1205 1237 1328 1360 4541 4573
Size: 32 32 495 32 91 32 3181 32
Cuts at: 4573 646
Size: 1097
Fragments arranged by size:
3181 1097 495 91 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 2821 2821
Size: 5024
XbaI T'CTAG_A
Cuts at: 2055 2055
Size: 5024
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 535 2519 114
Size: 421 1984 2619
Fragments arranged by size:
2619 1984 421
XmaI C'CCGG_G
Cuts at: 1501 2044 1501
Size: 543 4481
XmnI GAAnn'nnTTC
Cuts at: 363 934 363
Size: 571 4453
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII BstXI BstZ17I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI