Restriction map for
pF1KE1355
(Circular) (Six-base) MAPSORT of: pF1KE1355.seq Check: 4044 from: 1 to: 3959
pF1KE1355 3959 bp
With 182 enzymes: SgfI *
November 21, 2014 11:13 ..
AatII G_ACGT'C
Cuts at: 623 662 623
Size: 39 3920
AccI GT'mk_AC
Cuts at: 623 997 623
Size: 374 3585
Acc65I G'GTAC_C
Cuts at: 975 975
Size: 3959
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 970 1784 2216 2963 3231 196
Size: 774 814 432 747 268 924
Fragments arranged by size:
924 814 774 747 432 268
AfeI AGC'GCT
Cuts at: 409 771 409
Size: 362 3597
AflIII A'CryG_T
Cuts at: 1311 2415 3392 1311
Size: 1104 977 1878
Fragments arranged by size:
1878 1104 977
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AhdI GACnn_n'nnGTC
Cuts at: 438 438
Size: 3959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 258 756 1264 2831 258
Size: 498 508 1567 1386
Fragments arranged by size:
1567 1386 508 498
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 963 3312 3568 963
Size: 2349 256 1354
Fragments arranged by size:
2349 1354 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 582 979 582
Size: 397 3562
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 841 975 1389 1637 1672 841
Size: 134 414 248 35 3128
Fragments arranged by size:
3128 414 248 134 35
BanII G_rGCy'C
Cuts at: 482 973 2003 482
Size: 491 1030 2438
Fragments arranged by size:
2438 1030 491
BbeI G_GCGC'C
Cuts at: 845 1393 1641 845
Size: 548 248 3163
Fragments arranged by size:
3163 548 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 664 698 664
Size: 34 3925
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 1479 1479
Size: 3959
BglII A'GATC_T
Cuts at: 1474 3511 1474
Size: 2037 1922
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 1584 1677 2733 1584
Size: 93 1056 2810
Fragments arranged by size:
2810 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 394 705 1171 1252 1587 3731 394
Size: 311 466 81 335 2144 622
Fragments arranged by size:
2144 622 466 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 3959
Bpu10I CC'TnA_GC
Cuts at: 259 259
Size: 3959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 696 3663 205
Size: 491 2967 501
Fragments arranged by size:
2967 501 491
BsaAI yAC'GTr
Cuts at: 369 1312 369
Size: 943 3016
BsaBI GATnn'nnATC
Cuts at: 6 1473 3510 6
Size: 1467 2037 455
Fragments arranged by size:
2037 1467 455
BsaHI Gr'CG_yC
Cuts at: 620 659 842 1390 1638 2340 3836 620
Size: 39 183 548 248 702 1496 743
Fragments arranged by size:
1496 743 702 548 248 183 39
BsaWI w'CCGG_w
Cuts at: 1148 1669 2347 2621 2768 3179 1148
Size: 521 678 274 147 411 1928
Fragments arranged by size:
1928 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 126 1384 2142 2719 3215 126
Size: 1258 758 577 496 870
Fragments arranged by size:
1258 870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3959
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 973 1751 1941 2733 973
Size: 778 190 792 2199
Fragments arranged by size:
2199 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 482 973 1584 1677 1751 1941 2003 2733 482
Size: 491 611 93 74 190 62 730 1708
Fragments arranged by size:
1708 730 611 491 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1009 1223 1525 1906 1009
Size: 214 302 381 3062
Fragments arranged by size:
3062 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2251 2305 3789 3954 50
Size: 2201 54 1484 165 55
Fragments arranged by size:
2201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1871 1871
Size: 3959
BsrFI r'CCGG_y
Cuts at: 1957 2138 2347 1957
Size: 181 209 3569
Fragments arranged by size:
3569 209 181
BsrGI T'GTAC_A
Cuts at: 422 422
Size: 3959
BssSI C'ACGA_G
Cuts at: 2230 2588 2230
Size: 358 3601
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstXI CCAn_nnnn'nTGG
Cuts at: 205 205
Size: 3959
BstYI r'GATC_y
Cuts at: 1 254 984 1451 1474 1809 2055 3067 3153
Size: 253 730 467 23 335 246 1012 86
Cuts at: 3153 3165 3511 1
Size: 12 346 449
Fragments arranged by size:
1012 730 467 449 346 335 253 246
86 23 12
BtgI C'CryG_G
Cuts at: 314 314
Size: 3959
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 258 1909 2090 2253 258
Size: 1651 181 163 1964
Fragments arranged by size:
1964 1651 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DraIII CAC_nnn'GTG
Cuts at: 369 369
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 219 1665 2523 219
Size: 1446 858 1655
Fragments arranged by size:
1655 1446 858
EaeI y'GGCC_r
Cuts at: 1544 1718 2109 2136 3481 1544
Size: 174 391 27 1345 2022
Fragments arranged by size:
2022 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 869 1982 2192 869
Size: 1113 210 2636
Fragments arranged by size:
2636 1113 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 715 2167 2477 2623 3769 715
Size: 1452 310 146 1146 905
Fragments arranged by size:
1452 1146 905 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 970 1784 2216 2963 3231 196
Size: 774 814 432 747 268 924
Fragments arranged by size:
924 814 774 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 157 283 340 807 157
Size: 126 57 467 3309
Fragments arranged by size:
3309 467 126 57
EcoO109I rG'GnC_Cy
Cuts at: 1098 1098
Size: 3959
EcoRI G'AATT_C
Cuts at: 963 963
Size: 3959
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 136 411 773 845 1393 1641 2663 3642 136
Size: 275 362 72 548 248 1022 979 453
Fragments arranged by size:
1022 979 548 453 362 275 248 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 594 626 1801 1831 1833 1863 424
Size: 32 138 32 1175 30 2 30 2520
Fragments arranged by size:
2520 1175 138 32 32 30 30 2
HincII GTy'rAC
Cuts at: 998 998
Size: 3959
KasI G'GCGC_C
Cuts at: 841 1389 1637 841
Size: 548 248 3163
Fragments arranged by size:
3163 548 248
KpnI G_GTAC'C
Cuts at: 979 979
Size: 3959
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2630 2814 2630
Size: 184 3775
MscI TGG'CCA
Cuts at: 1720 1720
Size: 3959
MslI CAynn'nnrTG
Cuts at: 667 1948 2075 667
Size: 1281 127 2551
Fragments arranged by size:
2551 1281 127
MspA1I CmG'CkG
Cuts at: 1070 1384 1744 2757 3002 1070
Size: 314 360 1013 245 2027
Fragments arranged by size:
2027 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 842 1390 1638 842
Size: 548 248 3163
Fragments arranged by size:
3163 548 248
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NspI r_CATG'y
Cuts at: 577 1012 1512 2419 577
Size: 435 500 907 2117
Fragments arranged by size:
2117 907 500 435
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PfoI T'CCnGG_A
Cuts at: 284 284
Size: 3959
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3927
PstI C_TGCA'G
Cuts at: 161 694 1006 1691 161
Size: 533 312 685 2429
Fragments arranged by size:
2429 685 533 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 869 1982 2192 869
Size: 1113 210 2636
Fragments arranged by size:
2636 1113 210
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SexAI A'CCwGG_T
Cuts at: 434 434
Size: 3959
SfcI C'TryA_G
Cuts at: 33 157 690 1002 1687 2680 2871 33
Size: 124 533 312 685 993 191 1121
Fragments arranged by size:
1121 993 685 533 312 191 124
SfoI GGC'GCC
Cuts at: 843 1391 1639 843
Size: 548 248 3163
Fragments arranged by size:
3163 548 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 584 981 584
Size: 397 3562
SmlI C'TyrA_G
Cuts at: 1114 2029 2521 2783 1114
Size: 915 492 262 2290
Fragments arranged by size:
2290 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 577 1012 1512 577
Size: 435 500 3024
Fragments arranged by size:
3024 500 435
StuI AGG'CCT
Cuts at: 429 429
Size: 3959
StyI C'CwwG_G
Cuts at: 1093 1447 1093
Size: 354 3605
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 1542 1675 2134 2343 804
Size: 738 133 459 209 2420
Fragments arranged by size:
2420 738 459 209 133
TatI w'GTAC_w
Cuts at: 422 3474 422
Size: 3052 907
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 3927
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 886 1454 886
Size: 568 3391
XmaI C'CCGG_G
Cuts at: 582 979 582
Size: 397 3562
ZraI GAC'GTC
Cuts at: 621 660 621
Size: 39 3920
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AleI AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI