Restriction map for
pF1KE1360
(Circular) (Six-base) MAPSORT of: pF1KE1360.seq Check: 5131 from: 1 to: 3830
pF1KE1360 3830 bp
With 182 enzymes: SgfI *
January 7, 2013 14:38 ..
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 1655 2087 2834 3102 150
Size: 1505 432 747 268 878
Fragments arranged by size:
1505 878 747 432 268
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlwNI CAG_nnn'CTG
Cuts at: 1135 2702 1135
Size: 1567 2263
ApaI G_GGCC'C
Cuts at: 294 294
Size: 3830
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 196 834 3183 3439 196
Size: 638 2349 256 587
Fragments arranged by size:
2349 638 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 850 850
Size: 3830
BamHI G'GATC_C
Cuts at: 479 855 479
Size: 376 3454
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 294 713 844 1874 294
Size: 419 131 1030 2250
Fragments arranged by size:
2250 1030 419 131
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BbsI GAAGACnn'nnnn_
Cuts at: 156 156
Size: 3830
BbvCI CC'TCA_GC
Cuts at: 191 191
Size: 3830
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BfrBI ATG'CAT
Cuts at: 461 461
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 749 942 749
Size: 193 3637
Bme1580I G_kGCm'C
Cuts at: 294 1455 1548 2604 294
Size: 1161 93 1056 1520
Fragments arranged by size:
1520 1161 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1042 1123 1458 3602 1042
Size: 81 335 2144 1270
Fragments arranged by size:
2144 1270 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 799 568
Size: 231 3599
Bpu10I CC'TnA_GC
Cuts at: 133 191 712 133
Size: 58 521 3251
Fragments arranged by size:
3251 521 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1885 2377 2675 1006
Size: 879 492 298 2161
Fragments arranged by size:
2161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3534 3534
Size: 3830
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 1261 1509 2211 3707 1261
Size: 248 702 1496 1384
Fragments arranged by size:
1496 1384 702 248
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1255 2013 2590 3086 1255
Size: 758 577 496 1999
Fragments arranged by size:
1999 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1372 1418 2626 104
Size: 1268 46 1208 1308
Fragments arranged by size:
1308 1268 1208 46
BsiHKAI G_wGCw'C
Cuts at: 713 844 1622 1812 2604 713
Size: 131 778 190 792 1939
Fragments arranged by size:
1939 792 778 190 131
BsmI GAATG_Cn'
Cuts at: 461 564 461
Size: 103 3727
Bsp1286I G_dGCh'C
Cuts at: 294 713 844 1455 1548 1622 1812 1874 2604
Size: 419 131 611 93 74 190 62 730
Cuts at: 2604 294
Size: 1520
Fragments arranged by size:
1520 730 611 419 190 131 93 74
62
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1094 1396 1777 880
Size: 214 302 381 2933
Fragments arranged by size:
2933 381 302 214
BsrBI CCG'CTC
Cuts at: 50 137 2122 2176 3660 3825 50
Size: 87 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 87 55 54
BsrDI GCAATG_nn'
Cuts at: 1742 1742
Size: 3830
BsrFI r'CCGG_y
Cuts at: 1828 2009 2218 1828
Size: 181 209 3440
Fragments arranged by size:
3440 209 181
BssSI C'ACGA_G
Cuts at: 2101 2459 2101
Size: 358 3472
BstAPI GCAn_nnn'nTGC
Cuts at: 1207 1207
Size: 3830
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 440 726 440
Size: 286 3544
BstYI r'GATC_y
Cuts at: 1 479 855 1322 1345 1680 1926 2938 3024
Size: 478 376 467 23 335 246 1012 86
Cuts at: 3024 3036 3382 1
Size: 12 346 449
Fragments arranged by size:
1012 478 467 449 376 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 733 733
Size: 3830
BtgI C'CryG_G
Cuts at: 107 719 107
Size: 612 3218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1780 1961 2124 1780
Size: 181 163 3486
Fragments arranged by size:
3486 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1073
Size: 3830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 102 137 102
Size: 35 3795
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 110 632 1415 1589 1980 2007 3352 110
Size: 522 783 174 391 27 1345 588
Fragments arranged by size:
1345 783 588 522 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2038 2348 2494 3640 2038
Size: 310 146 1146 2228
Fragments arranged by size:
2228 1146 310 146
EcoICRI GAG'CTC
Cuts at: 711 842 711
Size: 131 3699
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 568 799 1655 2087 2834 3102 150
Size: 418 231 856 432 747 268 878
Fragments arranged by size:
878 856 747 432 418 268 231
EcoO109I rG'GnC_Cy
Cuts at: 291 737 969 291
Size: 446 232 3152
Fragments arranged by size:
3152 446 232
EcoRI G'AATT_C
Cuts at: 196 834 196
Size: 638 3192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 1264 1512 2534 3513 1264
Size: 248 1022 979 1581
Fragments arranged by size:
1581 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1672 1702 1704 1734 1672
Size: 30 2 30 3768
Fragments arranged by size:
3768 30 30 2
HincII GTy'rAC
Cuts at: 792 869 792
Size: 77 3753
HindIII A'AGCT_T
Cuts at: 581 581
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 507 2501 2685 507
Size: 1994 184 1652
Fragments arranged by size:
1994 1652 184
MscI TGG'CCA
Cuts at: 112 634 1591 112
Size: 522 957 2351
Fragments arranged by size:
2351 957 522
MslI CAynn'nnrTG
Cuts at: 466 1819 1946 466
Size: 1353 127 2350
Fragments arranged by size:
2350 1353 127
MspA1I CmG'CkG
Cuts at: 941 1255 1615 2628 2873 941
Size: 314 360 1013 245 1898
Fragments arranged by size:
1898 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 719 107
Size: 612 3218
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NsiI A_TGCA'T
Cuts at: 463 463
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PasI CC'CwG_GG
Cuts at: 391 391
Size: 3830
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PfoI T'CCnGG_A
Cuts at: 182 533 182
Size: 351 3479
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PspOMI G'GGCC_C
Cuts at: 290 290
Size: 3830
PstI C_TGCA'G
Cuts at: 877 1562 877
Size: 685 3145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 1255 1615 1255
Size: 360 3470
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 713 844 713
Size: 131 3699
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 607 3347 607
Size: 2740 1090
SfcI C'TryA_G
Cuts at: 33 204 873 1558 2551 2742 33
Size: 171 669 685 993 191 1121
Fragments arranged by size:
1121 993 685 669 191 171
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 985 1900 2392 2654 985
Size: 915 492 262 2161
Fragments arranged by size:
2161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StuI AGG'CCT
Cuts at: 640 640
Size: 3830
StyI C'CwwG_G
Cuts at: 107 326 635 644 674 719 964 1318 107
Size: 219 309 9 30 45 245 354 2619
Fragments arranged by size:
2619 354 309 245 219 45 30 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 1413 1546 2005 2214 339
Size: 1074 133 459 209 1955
Fragments arranged by size:
1955 1074 459 209 133
TatI w'GTAC_w
Cuts at: 605 3345 605
Size: 2740 1090
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 3347 3379 287
Size: 32 3028 32 738
Fragments arranged by size:
3028 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1325 1325
Size: 3830
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
XmnI GAAnn'nnTTC
Cuts at: 178 178
Size: 3830
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI