Restriction map for
pF1KE1394
(Circular) (Six-base) MAPSORT of: pF1KE1394.seq Check: 837 from: 1 to: 4679
pF1KE1394 4679 bp
With 182 enzymes: SgfI *
January 28, 2010 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 780 1260 780
Size: 480 4199
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1179 1364 1579 2504 2936 3683 3951 1172
Size: 7 185 215 925 432 747 268 1900
Fragments arranged by size:
1900 925 747 432 268 215 185 7
AflII C'TTAA_G
Cuts at: 624 624
Size: 4679
AflIII A'CryG_T
Cuts at: 2031 3135 4112 2031
Size: 1104 977 2598
Fragments arranged by size:
2598 1104 977
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AleI CACnn'nnGTG
Cuts at: 815 815
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 781 1984 3551 781
Size: 1203 1567 1909
Fragments arranged by size:
1909 1567 1203
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 154 260 1410 1683 4032 4288 154
Size: 106 1150 273 2349 256 545
Fragments arranged by size:
2349 1150 545 273 256 106
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1699 1699
Size: 4679
BamHI G'GATC_C
Cuts at: 305 825 1704 305
Size: 520 879 3280
Fragments arranged by size:
3280 879 520
BanI G'GyrC_C
Cuts at: 1695 2109 2357 2392 1695
Size: 414 248 35 3982
Fragments arranged by size:
3982 414 248 35
BanII G_rGCy'C
Cuts at: 1693 2723 1693
Size: 1030 3649
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BbsI GAAGACnn'nnnn_
Cuts at: 707 1118 707
Size: 411 4268
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3338 4679 2577
Size: 761 1341 2577
Fragments arranged by size:
2577 1341 761
BclI T'GATC_A
Cuts at: 2199 2199
Size: 4679
BfrBI ATG'CAT
Cuts at: 207 207
Size: 4679
BglI GCCn_nnn'nGGC
Cuts at: 355 355
Size: 4679
BglII A'GATC_T
Cuts at: 592 2194 4231 592
Size: 1602 2037 1040
Fragments arranged by size:
2037 1602 1040
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 2304 2397 3453 2304
Size: 93 1056 3530
Fragments arranged by size:
3530 1056 93
BmgBI CAC'GTC
Cuts at: 1029 1029
Size: 4679
BmrI ACTGGGnnnn_n'
Cuts at: 862 1891 1972 2307 4451 862
Size: 1029 81 335 2144 1090
Fragments arranged by size:
2144 1090 1029 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 1363 275
Size: 1088 3591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 2734 3226 3524 1855
Size: 879 492 298 3010
Fragments arranged by size:
3010 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4383 4383
Size: 4679
BsaAI yAC'GTr
Cuts at: 792 2032 792
Size: 1240 3439
BsaBI GATnn'nnATC
Cuts at: 6 304 2193 4230 6
Size: 298 1889 2037 455
Fragments arranged by size:
2037 1889 455 298
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 273 303 395 425 273
Size: 30 92 30 4527
Fragments arranged by size:
4527 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 1006 1360 278
Size: 728 354 3597
Fragments arranged by size:
3597 728 354
BseYI C'CCAG_C
Cuts at: 2104 2862 3439 3935 2104
Size: 758 577 496 2848
Fragments arranged by size:
2848 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3475 104
Size: 2117 46 1208 1308
Fragments arranged by size:
2117 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1251 1693 2471 2661 3453 1251
Size: 442 778 190 792 2477
Fragments arranged by size:
2477 792 778 442 190
Bsp1286I G_dGCh'C
Cuts at: 1251 1693 2304 2397 2471 2661 2723 3453 1251
Size: 442 611 93 74 190 62 730 2477
Fragments arranged by size:
2477 730 611 442 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 860 3855 860
Size: 2995 1684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 780 1260 1729 1943 2245 2626 780
Size: 480 469 214 302 381 2833
Fragments arranged by size:
2833 480 469 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 189 335 554 839 1602 2591 189
Size: 146 219 285 763 989 2277
Fragments arranged by size:
2277 989 763 285 219 146
BsrFI r'CCGG_y
Cuts at: 1143 2677 2858 3067 1143
Size: 1534 181 209 2755
Fragments arranged by size:
2755 1534 209 181
BsrGI T'GTAC_A
Cuts at: 1400 1400
Size: 4679
BssSI C'ACGA_G
Cuts at: 2950 3308 2950
Size: 358 4321
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2056
Size: 4679
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstXI CCAn_nnnn'nTGG
Cuts at: 852 852
Size: 4679
BstYI r'GATC_y
Cuts at: 1 305 592 721 825 1573 1704 2171 2194
Size: 304 287 129 104 748 131 467 23
Cuts at: 2194 2529 2775 3787 3873 3885 4231 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 748 467 449 346 335 304 287
246 131 129 104 86 23 12
BtgI C'CryG_G
Cuts at: 536 1073 536
Size: 537 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1922 1922
Size: 4679
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3243 2385
Size: 858 3821
EaeI y'GGCC_r
Cuts at: 533 2264 2438 2829 2856 4201 533
Size: 1731 174 391 27 1345 1011
Fragments arranged by size:
1731 1345 1011 391 174 27
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 1089 1257 1517 2702 2912 1089
Size: 168 260 1185 210 2856
Fragments arranged by size:
2856 1185 260 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3197 3343 4489 2887
Size: 310 146 1146 3077
Fragments arranged by size:
3077 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 1172 1179 1363 1364 1579 2504 2936 3683
Size: 897 7 184 1 215 925 432 747
Cuts at: 3683 3951 275
Size: 268 1003
Fragments arranged by size:
1003 925 897 747 432 268 215 184
7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 425 425
Size: 4679
EcoO109I rG'GnC_Cy
Cuts at: 1818 1818
Size: 4679
EcoRI G'AATT_C
Cuts at: 154 1683 154
Size: 1529 3150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 2113 2361 3383 4362 2113
Size: 248 1022 979 2430
Fragments arranged by size:
2430 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 273 305 2521 2551 2553 2583 206
Size: 32 35 32 2216 30 2 30 2302
Fragments arranged by size:
2302 2216 35 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1065 1718 1065
Size: 653 4026
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MfeI C'AATT_G
Cuts at: 677 677
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1629 3350 3534 1629
Size: 1721 184 2774
Fragments arranged by size:
2774 1721 184
MscI TGG'CCA
Cuts at: 535 2440 535
Size: 1905 2774
MslI CAynn'nnrTG
Cuts at: 325 661 815 965 2668 2795 325
Size: 336 154 150 1703 127 2209
Fragments arranged by size:
2209 1703 336 154 150 127
MspA1I CmG'CkG
Cuts at: 1217 1790 2104 2464 3477 3722 1217
Size: 573 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 573 360 314 245
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NcoI C'CATG_G
Cuts at: 536 1073 536
Size: 537 4142
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NsiI A_TGCA'T
Cuts at: 209 209
Size: 4679
NspI r_CATG'y
Cuts at: 211 1732 2232 3139 211
Size: 1521 500 907 1751
Fragments arranged by size:
1751 1521 907 500
PciI A'CATG_T
Cuts at: 3135 3135
Size: 4679
PfoI T'CCnGG_A
Cuts at: 291 291
Size: 4679
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PsiI TTA'TAA
Cuts at: 589 589
Size: 4679
PstI C_TGCA'G
Cuts at: 777 1017 1581 1726 2411 777
Size: 240 564 145 685 3045
Fragments arranged by size:
3045 685 564 240 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 2104 2464 2104
Size: 360 4319
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 1257 2702 2912 1257
Size: 1445 210 3024
Fragments arranged by size:
3024 1445 210
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SfcI C'TryA_G
Cuts at: 33 773 1013 1426 1506 1577 1722 2407 3400
Size: 740 240 413 80 71 145 685 993
Cuts at: 3400 3591 33
Size: 191 1121
Fragments arranged by size:
1121 993 740 685 413 240 191 145
80 71
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 624 1834 2749 3241 3503 624
Size: 1210 915 492 262 1800
Fragments arranged by size:
1800 1210 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
SspI AAT'ATT
Cuts at: 942 942
Size: 4679
StuI AGG'CCT
Cuts at: 120 147 120
Size: 27 4652
StyI C'CwwG_G
Cuts at: 536 1073 1813 2167 536
Size: 537 740 354 3048
Fragments arranged by size:
3048 740 537 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2262 2395 2854 3063 2262
Size: 133 459 209 3878
Fragments arranged by size:
3878 459 209 133
TatI w'GTAC_w
Cuts at: 849 1400 1526 4194 849
Size: 551 126 2668 1334
Fragments arranged by size:
2668 1334 551 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 776 808 1131 1163 4196 4228 776
Size: 32 323 32 3033 32 1227
Fragments arranged by size:
3033 1227 323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 263 1157 263
Size: 894 3785
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI