Restriction map for
pF1KE1398
(Circular) (Six-base) MAPSORT of: pF1KE1398.seq Check: 5770 from: 1 to: 6419
pF1KE1398 6419 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 1118 1658 3457 1118
Size: 540 1799 4080
Fragments arranged by size:
4080 1799 540
Acc65I G'GTAC_C
Cuts at: 3435 3435
Size: 6419
AclI AA'CG_TT
Cuts at: 3112 3413 6259 3112
Size: 301 2846 3272
Fragments arranged by size:
3272 2846 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 541 4244 4676 5423 5691 324
Size: 217 3703 432 747 268 1052
Fragments arranged by size:
3703 1052 747 432 268 217
AfeI AGC'GCT
Cuts at: 337 337
Size: 6419
AflII C'TTAA_G
Cuts at: 2749 2749
Size: 6419
AflIII A'CryG_T
Cuts at: 1649 1853 2357 3771 4875 5852 1649
Size: 204 504 1414 1104 977 2216
Fragments arranged by size:
2216 1414 1104 977 504 204
AgeI A'CCGG_T
Cuts at: 4807 4807
Size: 6419
AhdI GACnn_n'nnGTC
Cuts at: 1125 1560 1620 1125
Size: 435 60 5924
Fragments arranged by size:
5924 435 60
AlwNI CAG_nnn'CTG
Cuts at: 3186 3724 5291 3186
Size: 538 1567 4314
Fragments arranged by size:
4314 1567 538
ApaLI G'TGCA_C
Cuts at: 5189 5189
Size: 6419
ApoI r'AATT_y
Cuts at: 1288 1384 1411 1543 1964 2328 2342 3223 3282
Size: 96 27 132 421 364 14 881 59
Cuts at: 3282 3423 5772 6028 1288
Size: 141 2349 256 1679
Fragments arranged by size:
2349 1679 881 421 364 256 141 132
96 59 27 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 6419
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6419
AvaI C'yCGr_G
Cuts at: 166 2107 3439 166
Size: 1941 1332 3146
Fragments arranged by size:
3146 1941 1332
BamHI G'GATC_C
Cuts at: 3444 3444
Size: 6419
BanI G'GyrC_C
Cuts at: 1045 1060 3435 3849 4097 4132 1045
Size: 15 2375 414 248 35 3332
Fragments arranged by size:
3332 2375 414 248 35 15
BanII G_rGCy'C
Cuts at: 167 2060 3344 3433 4463 167
Size: 1893 1284 89 1030 2123
Fragments arranged by size:
2123 1893 1284 1030 89
BbeI G_GCGC'C
Cuts at: 3853 4101 3853
Size: 248 6171
BbsI GAAGACnn'nnnn_
Cuts at: 3096 3272 3096
Size: 176 6243
BbvCI CC'TCA_GC
Cuts at: 1718 3133 1718
Size: 1415 5004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2098 2132 2098
Size: 34 6385
BciVI GTATCCnnnnn_n'
Cuts at: 3131 4317 5078 6419 3131
Size: 1186 761 1341 3131
Fragments arranged by size:
3131 1341 1186 761
BclI T'GATC_A
Cuts at: 230 3939 230
Size: 3709 2710
BglII A'GATC_T
Cuts at: 2472 2694 3934 5971 2472
Size: 222 1240 2037 2920
Fragments arranged by size:
2920 2037 1240 222
BlpI GC'TnA_GC
Cuts at: 3531 3531
Size: 6419
Bme1580I G_kGCm'C
Cuts at: 1063 4044 4137 5193 1063
Size: 2981 93 1056 2289
Fragments arranged by size:
2981 2289 1056 93
BmgBI CAC'GTC
Cuts at: 491 1557 2358 491
Size: 1066 801 4552
Fragments arranged by size:
4552 1066 801
BmrI ACTGGGnnnn_n'
Cuts at: 2254 3631 3712 4047 6191 2254
Size: 1377 81 335 2144 2482
Fragments arranged by size:
2482 2144 1377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1033 1952 4786 1033
Size: 919 2834 2666
Fragments arranged by size:
2834 2666 919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 645 448
Size: 197 6222
Bpu10I CC'TnA_GC
Cuts at: 364 1718 3133 364
Size: 1354 1415 3650
Fragments arranged by size:
3650 1415 1354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3595 4474 4966 5264 2618
Size: 977 879 492 298 3773
Fragments arranged by size:
3773 977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6123 6123
Size: 6419
BsaAI yAC'GTr
Cuts at: 2248 3772 2248
Size: 1524 4895
BsaBI GATnn'nnATC
Cuts at: 6 2150 2758 3933 5970 6
Size: 2144 608 1175 2037 455
Fragments arranged by size:
2144 2037 1175 608 455
BsaHI Gr'CG_yC
Cuts at: 3850 4098 4800 6296 3850
Size: 248 702 1496 3973
Fragments arranged by size:
3973 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3608 4129 4807 5081 5228 5639 3608
Size: 521 678 274 147 411 4388
Fragments arranged by size:
4388 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3193 3223 3193
Size: 30 6389
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 1896 3384 289
Size: 1607 1488 3324
Fragments arranged by size:
3324 1607 1488
BseYI C'CCAG_C
Cuts at: 1249 3844 4602 5179 5675 1249
Size: 2595 758 577 496 1993
Fragments arranged by size:
2595 1993 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 1828 1620
Size: 208 6211
BsiEI CG_ry'CG
Cuts at: 104 3961 4007 5215 104
Size: 3857 46 1208 1308
Fragments arranged by size:
3857 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1595 2060 3433 4211 4401 5193 1595
Size: 465 1373 778 190 792 2821
Fragments arranged by size:
2821 1373 792 778 465 190
Bsp1286I G_dGCh'C
Cuts at: 167 1063 1595 2060 3344 3433 4044 4137 4211
Size: 896 532 465 1284 89 611 93 74
Cuts at: 4211 4401 4463 5193 167
Size: 190 62 730 1393
Fragments arranged by size:
1393 1284 896 730 611 532 465 190
93 89 74 62
BspEI T'CCGG_A
Cuts at: 5639 5639
Size: 6419
BspHI T'CATG_A
Cuts at: 2154 5595 2154
Size: 3441 2978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3469 3683 3985 4366 3469
Size: 214 302 381 5522
Fragments arranged by size:
5522 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1779 4711 4765 6249 6414 50
Size: 1729 2932 54 1484 165 55
Fragments arranged by size:
2932 1729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 477 1297 2021 4331 477
Size: 820 724 2310 2565
Fragments arranged by size:
2565 2310 820 724
BsrFI r'CCGG_y
Cuts at: 4417 4598 4807 4417
Size: 181 209 6029
Fragments arranged by size:
6029 209 181
BsrGI T'GTAC_A
Cuts at: 690 833 690
Size: 143 6276
BssSI C'ACGA_G
Cuts at: 4690 5048 4690
Size: 358 6061
BstAPI GCAn_nnn'nTGC
Cuts at: 3796 3796
Size: 6419
BstBI TT'CG_AA
Cuts at: 3018 4780 3018
Size: 1762 4657
BstEII G'GTnAC_C
Cuts at: 1698 1698
Size: 6419
BstXI CCAn_nnnn'nTGG
Cuts at: 618 1834 618
Size: 1216 5203
BstYI r'GATC_y
Cuts at: 1 1428 2472 2604 2694 3444 3911 3934 4269
Size: 1427 1044 132 90 750 467 23 335
Cuts at: 4269 4515 5527 5613 5625 5971 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1427 1044 1012 750 467 449 346 335
246 132 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1433 1433
Size: 6419
BtgI C'CryG_G
Cuts at: 269 394 708 1080 1943 2891 269
Size: 125 314 372 863 948 3797
Fragments arranged by size:
3797 948 863 372 314 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4369 4550 4713 4369
Size: 181 163 6075
Fragments arranged by size:
6075 181 163
BtsI GCAGTG_nn'
Cuts at: 521 1632 3662 521
Size: 1111 2030 3278
Fragments arranged by size:
3278 2030 1111
DraI TTT'AAA
Cuts at: 2790 3222 3418 2790
Size: 432 196 5791
Fragments arranged by size:
5791 432 196
DrdI GACnn_nn'nnGTC
Cuts at: 4125 4983 4125
Size: 858 5561
EaeI y'GGCC_r
Cuts at: 1836 4004 4178 4569 4596 5941 1836
Size: 2168 174 391 27 1345 2314
Fragments arranged by size:
2314 2168 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4004 4004
Size: 6419
EarI CTCTTCn'nnn_
Cuts at: 1131 1739 2816 4442 4652 1131
Size: 608 1077 1626 210 2898
Fragments arranged by size:
2898 1626 1077 608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 712 4627 4937 5083 6229 712
Size: 3915 310 146 1146 902
Fragments arranged by size:
3915 1146 902 310 146
EcoICRI GAG'CTC
Cuts at: 2058 3431 2058
Size: 1373 5046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 448 541 645 4244 4676 5423 5691 324
Size: 124 93 104 3599 432 747 268 1052
Fragments arranged by size:
3599 1052 747 432 268 124 104 93
EcoNI CCTnn'n_nnAGG
Cuts at: 647 2311 3335 3391 647
Size: 1664 1024 56 3675
Fragments arranged by size:
3675 1664 1024 56
EcoO109I rG'GnC_Cy
Cuts at: 1437 2865 3558 1437
Size: 1428 693 4298
Fragments arranged by size:
4298 1428 693
EcoRI G'AATT_C
Cuts at: 1384 2342 3423 1384
Size: 958 1081 4380
Fragments arranged by size:
4380 1081 958
EcoRV GAT'ATC
Cuts at: 1155 1155
Size: 6419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 893 4438 893
Size: 3545 2874
FspI TGC'GCA
Cuts at: 4200 4200
Size: 6419
HaeII r_GCGC'y
Cuts at: 306 339 3853 4101 5123 6102 306
Size: 33 3514 248 1022 979 623
Fragments arranged by size:
3514 1022 979 623 248 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 446 475 478 507 535 567 847 879 1111
Size: 29 3 29 28 32 280 32 232
Cuts at: 1111 1143 1546 1578 1606 1638 1746 1778 2169
Size: 32 403 32 28 32 108 32 391
Cuts at: 2169 2201 2896 2928 4261 4291 4293 4323 446
Size: 32 695 32 1333 30 2 30 2542
Fragments arranged by size:
2542 1333 695 403 391 280 232 108
32 32 32 32 32 32 32 32
30 30 29 29 28 28 3 2
HincII GTy'rAC
Cuts at: 944 3458 944
Size: 2514 3905
HpaI GTT'AAC
Cuts at: 944 944
Size: 6419
KasI G'GCGC_C
Cuts at: 3849 4097 3849
Size: 248 6171
KpnI G_GTAC'C
Cuts at: 3439 3439
Size: 6419
MfeI C'AATT_G
Cuts at: 1586 3230 1586
Size: 1644 4775
MluI A'CGCG_T
Cuts at: 5852 5852
Size: 6419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5090 5274 5090
Size: 184 6235
MscI TGG'CCA
Cuts at: 1838 4180 1838
Size: 2342 4077
MslI CAynn'nnrTG
Cuts at: 178 957 1598 2675 2855 4408 4535 178
Size: 779 641 1077 180 1553 127 2062
Fragments arranged by size:
2062 1553 1077 779 641 180 127
MspA1I CmG'CkG
Cuts at: 271 1082 3530 3844 4204 5217 5462 271
Size: 811 2448 314 360 1013 245 1228
Fragments arranged by size:
2448 1228 1013 811 360 314 245
NaeI GCC'GGC
Cuts at: 4600 4600
Size: 6419
NarI GG'CG_CC
Cuts at: 3850 4098 3850
Size: 248 6171
NcoI C'CATG_G
Cuts at: 394 708 1943 394
Size: 314 1235 4870
Fragments arranged by size:
4870 1235 314
NgoMIV G'CCGG_C
Cuts at: 4598 4598
Size: 6419
NspI r_CATG'y
Cuts at: 1653 1857 3472 3972 4879 1653
Size: 204 1615 500 907 3193
Fragments arranged by size:
3193 1615 907 500 204
PciI A'CATG_T
Cuts at: 1649 1853 4875 1649
Size: 204 3022 3193
Fragments arranged by size:
3193 3022 204
PflMI CCAn_nnn'nTGG
Cuts at: 494 494
Size: 6419
PfoI T'CCnGG_A
Cuts at: 2090 2526 2861 3197 2090
Size: 436 335 336 5312
Fragments arranged by size:
5312 436 336 335
PmeI GTTT'AAAC
Cuts at: 3418 3418
Size: 6419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5558 5590 5558
Size: 32 6387
PpuMI rG'GwC_Cy
Cuts at: 1437 2865 1437
Size: 1428 4991
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1247 1279 1247
Size: 32 6387
PstI C_TGCA'G
Cuts at: 2621 3466 4151 2621
Size: 845 685 4889
Fragments arranged by size:
4889 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6419
PvuII CAG'CTG
Cuts at: 3844 4204 3844
Size: 360 6059
RsrII CG'GwC_CG
Cuts at: 4614 4614
Size: 6419
SacI G_AGCT'C
Cuts at: 2060 3433 2060
Size: 1373 5046
SacII CC_GC'GG
Cuts at: 272 1083 272
Size: 811 5608
SalI G'TCGA_C
Cuts at: 3456 3456
Size: 6419
SapI GCTCTTCn'nnn_
Cuts at: 4442 4652 4442
Size: 210 6209
SbfI CC_TGCA'GG
Cuts at: 3466 3466
Size: 6419
ScaI AGT'ACT
Cuts at: 5936 5936
Size: 6419
SexAI A'CCwGG_T
Cuts at: 3041 3041
Size: 6419
SfcI C'TryA_G
Cuts at: 33 793 926 2617 2701 3462 4147 5140 5331
Size: 760 133 1691 84 761 685 993 191
Cuts at: 5331 33
Size: 1121
Fragments arranged by size:
1691 1121 993 761 760 685 191 133
84
SfoI GGC'GCC
Cuts at: 3851 4099 3851
Size: 248 6171
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6419
SmaI CCC'GGG
Cuts at: 3441 3441
Size: 6419
SmlI C'TyrA_G
Cuts at: 2633 2749 3574 4489 4981 5243 2633
Size: 116 825 915 492 262 3809
Fragments arranged by size:
3809 915 825 492 262 116
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6419
SphI G_CATG'C
Cuts at: 3472 3972 3472
Size: 500 5919
SspI AAT'ATT
Cuts at: 2087 2087
Size: 6419
StyI C'CwwG_G
Cuts at: 394 708 1842 1943 2077 3331 3553 3907 394
Size: 314 1134 101 134 1254 222 354 2906
Fragments arranged by size:
2906 1254 1134 354 314 222 134 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 348 652 947 1115 2843 4002 4135 4594 4803
Size: 304 295 168 1728 1159 133 459 209
Cuts at: 4803 348
Size: 1964
Fragments arranged by size:
1964 1728 1159 459 304 295 209 168
133
TatI w'GTAC_w
Cuts at: 690 833 1570 3254 5934 690
Size: 143 737 1684 2680 1175
Fragments arranged by size:
2680 1684 1175 737 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1487 1519 5936 5968 1487
Size: 32 4417 32 1938
Fragments arranged by size:
4417 1938 32 32
Tth111I GACn'n_nGTC
Cuts at: 4216 4216
Size: 6419
XbaI T'CTAG_A
Cuts at: 3450 3450
Size: 6419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3914 3914
Size: 6419
XmaI C'CCGG_G
Cuts at: 3439 3439
Size: 6419
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AleI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BsmI BsmBI BssHII
BstZ17I ClaI CspCI DraIII FseI FspAI HindIII NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI