Restriction map for
pF1KE1415
(Circular) (Six-base) MAPSORT of: pF1KE1415.seq Check: 3432 from: 1 to: 3884
pF1KE1415 3884 bp
With 182 enzymes: SgfI *
August 9, 2016 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 396 427 453 568 396
Size: 31 26 115 3712
Fragments arranged by size:
3712 115 31 26
AccI GT'mk_AC
Cuts at: 279 922 279
Size: 643 3241
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 1709 2141 2888 3156 581
Size: 1128 432 747 268 1309
Fragments arranged by size:
1309 1128 747 432 268
AflIII A'CryG_T
Cuts at: 1236 2340 3317 1236
Size: 1104 977 1803
Fragments arranged by size:
1803 1104 977
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 3884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 3884
AlwNI CAG_nnn'CTG
Cuts at: 135 1189 2756 135
Size: 1054 1567 1263
Fragments arranged by size:
1567 1263 1054
ApaI G_GGCC'C
Cuts at: 452 821 452
Size: 369 3515
ApaLI G'TGCA_C
Cuts at: 2654 2654
Size: 3884
ApoI r'AATT_y
Cuts at: 888 3237 3493 888
Size: 2349 256 1279
Fragments arranged by size:
2349 1279 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 253 904 253
Size: 651 3233
BamHI G'GATC_C
Cuts at: 455 909 455
Size: 454 3430
BanI G'GyrC_C
Cuts at: 384 900 1314 1562 1597 384
Size: 516 414 248 35 2671
Fragments arranged by size:
2671 516 414 248 35
BanII G_rGCy'C
Cuts at: 452 821 898 1928 452
Size: 369 77 1030 2408
Fragments arranged by size:
2408 1030 369 77
BbeI G_GCGC'C
Cuts at: 1318 1566 1318
Size: 248 3636
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2543 3884 1782
Size: 761 1341 1782
Fragments arranged by size:
1782 1341 761
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 1399 3436 1399
Size: 2037 1847
BlpI GC'TnA_GC
Cuts at: 113 844 874 996 113
Size: 731 30 122 3001
Fragments arranged by size:
3001 731 122 30
Bme1580I G_kGCm'C
Cuts at: 452 506 821 1509 1602 2658 452
Size: 54 315 688 93 1056 1678
Fragments arranged by size:
1678 1056 688 315 93 54
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 306
Size: 3884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2431 2729 1060
Size: 879 492 298 2215
Fragments arranged by size:
2215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 1315 1563 2265 3761 1315
Size: 248 702 1496 1438
Fragments arranged by size:
1496 1438 702 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2546 2693 3104 1073
Size: 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 3854
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 790
Size: 3884
BseYI C'CCAG_C
Cuts at: 753 814 1309 2067 2644 3140 753
Size: 61 495 758 577 496 1497
Fragments arranged by size:
1497 758 577 496 495 61
BsiEI CG_ry'CG
Cuts at: 104 1426 1472 2680 104
Size: 1322 46 1208 1308
Fragments arranged by size:
1322 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 436 898 1676 1866 2658 436
Size: 462 778 190 792 1662
Fragments arranged by size:
1662 792 778 462 190
BsmI GAATG_Cn'
Cuts at: 300 300
Size: 3884
Bsp1286I G_dGCh'C
Cuts at: 436 452 506 821 898 1509 1602 1676 1866
Size: 16 54 315 77 611 93 74 190
Cuts at: 1866 1928 2658 436
Size: 62 730 1662
Fragments arranged by size:
1662 730 611 315 190 93 77 74
62 54 16
BspEI T'CCGG_A
Cuts at: 3104 3104
Size: 3884
BspHI T'CATG_A
Cuts at: 3060 3060
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 396 427 453 568 934 1148 1450 1831 396
Size: 31 26 115 366 214 302 381 2449
Fragments arranged by size:
2449 381 366 302 214 115 31 26
BsrBI CCG'CTC
Cuts at: 50 489 2176 2230 3714 3879 50
Size: 439 1687 54 1484 165 55
Fragments arranged by size:
1687 1484 439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 535 681 1796 535
Size: 146 1115 2623
Fragments arranged by size:
2623 1115 146
BsrFI r'CCGG_y
Cuts at: 1882 2063 2272 1882
Size: 181 209 3494
Fragments arranged by size:
3494 209 181
BssSI C'ACGA_G
Cuts at: 2155 2513 2155
Size: 358 3526
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstYI r'GATC_y
Cuts at: 1 455 909 1376 1399 1734 1980 2992 3078
Size: 454 454 467 23 335 246 1012 86
Cuts at: 3078 3090 3436 1
Size: 12 346 449
Fragments arranged by size:
1012 467 454 454 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 280 623 1127 280
Size: 343 504 3037
Fragments arranged by size:
3037 504 343
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2448 1590
Size: 858 3026
EaeI y'GGCC_r
Cuts at: 726 1469 1643 2034 2061 3406 726
Size: 743 174 391 27 1345 1204
Fragments arranged by size:
1345 1204 743 391 174 27
EagI C'GGCC_G
Cuts at: 1469 1469
Size: 3884
EarI CTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2402 2548 3694 2092
Size: 310 146 1146 2282
Fragments arranged by size:
2282 1146 310 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 581 1709 2141 2888 3156 306
Size: 275 1128 432 747 268 1034
Fragments arranged by size:
1128 1034 747 432 275 268
EcoO109I rG'GnC_Cy
Cuts at: 250 448 449 818 1023 250
Size: 198 1 369 205 3111
Fragments arranged by size:
3111 369 205 198 1
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 615 1665 615
Size: 1050 2834
HaeII r_GCGC'y
Cuts at: 1318 1566 2588 3567 1318
Size: 248 1022 979 1635
Fragments arranged by size:
1635 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1726 1756 1758 1788 1726
Size: 30 2 30 3822
Fragments arranged by size:
3822 30 30 2
HincII GTy'rAC
Cuts at: 280 923 280
Size: 643 3241
KasI G'GCGC_C
Cuts at: 1314 1562 1314
Size: 248 3636
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2739 2555
Size: 184 3700
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 335 739 1873 2000 335
Size: 404 1134 127 2219
Fragments arranged by size:
2219 1134 404 127
MspA1I CmG'CkG
Cuts at: 117 132 731 995 1309 1669 2682 2927 117
Size: 15 599 264 314 360 1013 245 1074
Fragments arranged by size:
1074 1013 599 360 314 264 245 15
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 1315 1563 1315
Size: 248 3636
NcoI C'CATG_G
Cuts at: 107 107
Size: 3884
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 2344 937
Size: 500 907 2477
Fragments arranged by size:
2477 907 500
PasI CC'CwG_GG
Cuts at: 759 821 759
Size: 62 3822
PciI A'CATG_T
Cuts at: 2340 2340
Size: 3884
PflMI CCAn_nnn'nTGG
Cuts at: 465 465
Size: 3884
PfoI T'CCnGG_A
Cuts at: 410 410
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3852
PspOMI G'GGCC_C
Cuts at: 448 817 448
Size: 369 3515
PstI C_TGCA'G
Cuts at: 801 931 1616 801
Size: 130 685 3069
Fragments arranged by size:
3069 685 130
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 132 1309 1669 132
Size: 1177 360 2347
Fragments arranged by size:
2347 1177 360
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SalI G'TCGA_C
Cuts at: 278 921 278
Size: 643 3241
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 158 711 797 927 1612 2605 2796 33
Size: 125 553 86 130 685 993 191 1121
Fragments arranged by size:
1121 993 685 553 191 130 125 86
SfoI GGC'GCC
Cuts at: 1316 1564 1316
Size: 248 3636
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2446 2708 1039
Size: 915 492 262 2215
Fragments arranged by size:
2215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
StyI C'CwwG_G
Cuts at: 107 1018 1372 107
Size: 911 354 2619
Fragments arranged by size:
2619 911 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 141 675 1467 1600 2059 2268 141
Size: 534 792 133 459 209 1757
Fragments arranged by size:
1757 792 534 459 209 133
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 357 389 3401 3433 357
Size: 32 3012 32 808
Fragments arranged by size:
3012 808 32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 777 1379 777
Size: 602 3282
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI