Restriction map for
pF1KE1429
(Circular) (Six-base) MAPSORT of: pF1KE1429.seq Check: 5311 from: 1 to: 4721
pF1KE1429 4721 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 317 1759 317
Size: 1442 3279
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 295 2546 2978 3725 3993 196
Size: 99 2251 432 747 268 924
Fragments arranged by size:
2251 924 747 432 268 99
AflIII A'CryG_T
Cuts at: 1112 2073 3177 4154 1112
Size: 961 1104 977 1679
Fragments arranged by size:
1679 1104 977 961
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AhdI GACnn_n'nnGTC
Cuts at: 1464 1644 1464
Size: 180 4541
AleI CACnn'nnGTG
Cuts at: 541 1354 541
Size: 813 3908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 505 506 537 538 505
Size: 1 31 1 4688
Fragments arranged by size:
4688 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 369 1446 2026 3593 369
Size: 1077 580 1567 1497
Fragments arranged by size:
1567 1497 1077 580
ApaI G_GGCC'C
Cuts at: 550 550
Size: 4721
ApaLI G'TGCA_C
Cuts at: 484 841 3491 484
Size: 357 2650 1714
Fragments arranged by size:
2650 1714 357
ApoI r'AATT_y
Cuts at: 1279 1725 4074 4330 1279
Size: 446 2349 256 1670
Fragments arranged by size:
2349 1670 446 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 434 1741 434
Size: 1307 3414
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 1493 1737 2151 2399 2434 1493
Size: 244 414 248 35 3780
Fragments arranged by size:
3780 414 248 244 35
BanII G_rGCy'C
Cuts at: 550 866 1083 1400 1735 2765 550
Size: 316 217 317 335 1030 2506
Fragments arranged by size:
2506 1030 335 317 316 217
BbeI G_GCGC'C
Cuts at: 1497 2155 2403 1497
Size: 658 248 3815
Fragments arranged by size:
3815 658 248
BbsI GAAGACnn'nnnn_
Cuts at: 1144 1153 1144
Size: 9 4712
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3380 4721 2619
Size: 761 1341 2619
Fragments arranged by size:
2619 1341 761
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 488 550 845 916 2346 2439 3495 488
Size: 62 295 71 1430 93 1056 1714
Fragments arranged by size:
1714 1430 1056 295 93 71 62
BmgBI CAC'GTC
Cuts at: 1422 1422
Size: 4721
BmrI ACTGGGnnnn_n'
Cuts at: 1933 2014 2349 4493 1933
Size: 81 335 2144 2161
Fragments arranged by size:
2161 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 190 307 1003 1186 163
Size: 27 117 696 183 3698
Fragments arranged by size:
3698 696 183 117 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1897 2776 3268 3566 565
Size: 1332 879 492 298 1720
Fragments arranged by size:
1720 1332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 221 1517 4425 221
Size: 1296 2908 517
Fragments arranged by size:
2908 1296 517
BsaAI yAC'GTr
Cuts at: 2074 2074
Size: 4721
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 1494 2152 2400 3102 4598 1494
Size: 658 248 702 1496 1617
Fragments arranged by size:
1617 1496 702 658 248
BsaWI w'CCGG_w
Cuts at: 132 524 1910 2431 3109 3383 3530 3941 132
Size: 392 1386 521 678 274 147 411 912
Fragments arranged by size:
1386 912 678 521 411 392 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 358 388 1294 1324 163
Size: 30 165 30 906 30 3560
Fragments arranged by size:
3560 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 283 472 160
Size: 123 189 4409
Fragments arranged by size:
4409 189 123
BseYI C'CCAG_C
Cuts at: 187 425 499 1480 2146 2904 3481 3977 187
Size: 238 74 981 666 758 577 496 931
Fragments arranged by size:
981 931 758 666 577 496 238 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 514
Size: 4721
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 488 845 866 908 1735 2513 2703 3495 488
Size: 357 21 42 827 778 190 792 1714
Fragments arranged by size:
1714 827 792 778 357 190 42 21
BsmI GAATG_Cn'
Cuts at: 1571 1571
Size: 4721
Bsp1286I G_dGCh'C
Cuts at: 488 550 845 866 908 916 1083 1400 1735
Size: 62 295 21 42 8 167 317 335
Cuts at: 1735 2346 2439 2513 2703 2765 3495 488
Size: 611 93 74 190 62 730 1714
Fragments arranged by size:
1714 730 611 335 317 295 190 167
93 74 62 62 42 21 8
BspEI T'CCGG_A
Cuts at: 132 524 3941 132
Size: 392 3417 912
Fragments arranged by size:
3417 912 392
BspHI T'CATG_A
Cuts at: 3897 3897
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1771 1985 2287 2668 1771
Size: 214 302 381 3824
Fragments arranged by size:
3824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 813 2633 813
Size: 1820 2901
BsrFI r'CCGG_y
Cuts at: 2719 2900 3109 2719
Size: 181 209 4331
Fragments arranged by size:
4331 209 181
BsrGI T'GTAC_A
Cuts at: 962 962
Size: 4721
BssSI C'ACGA_G
Cuts at: 2992 3350 2992
Size: 358 4363
BstAPI GCAn_nnn'nTGC
Cuts at: 369 492 2098 369
Size: 123 1606 2992
Fragments arranged by size:
2992 1606 123
BstBI TT'CG_AA
Cuts at: 3082 3082
Size: 4721
BstXI CCAn_nnnn'nTGG
Cuts at: 1356 1356
Size: 4721
BstYI r'GATC_y
Cuts at: 1 503 591 782 1746 2213 2236 2571 2817
Size: 502 88 191 964 467 23 335 246
Cuts at: 2817 3829 3915 3927 4273 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 964 502 467 449 346 335 246
191 88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 646 646
Size: 4721
BtgI C'CryG_G
Cuts at: 107 107
Size: 4721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 843 1243 1964 843
Size: 400 721 3600
Fragments arranged by size:
3600 721 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1237 1272 1237
Size: 35 4686
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DraIII CAC_nnn'GTG
Cuts at: 492 1336 492
Size: 844 3877
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 869 1103 2306 2480 2871 2898 4243 869
Size: 234 1203 174 391 27 1345 1347
Fragments arranged by size:
1347 1345 1203 391 234 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 1426 1655 2744 2954 1426
Size: 229 1089 210 3193
Fragments arranged by size:
3193 1089 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1465 2929 3239 3385 4531 1465
Size: 1464 310 146 1146 1655
Fragments arranged by size:
1655 1464 1146 310 146
EcoICRI GAG'CTC
Cuts at: 864 1733 864
Size: 869 3852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 190 196 295 307 1003 1186 2546 2978
Size: 27 6 99 12 696 183 1360 432
Cuts at: 2978 3725 3993 163
Size: 747 268 891
Fragments arranged by size:
1360 891 747 696 432 268 183 99
27 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 1273 1054
Size: 219 4502
EcoO109I rG'GnC_Cy
Cuts at: 547 1188 1266 1665 1860 547
Size: 641 78 399 195 3408
Fragments arranged by size:
3408 641 399 195 78
EcoRI G'AATT_C
Cuts at: 1279 1725 1279
Size: 446 4275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1170 2740 1170
Size: 1570 3151
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 1497 2155 2403 3425 4404 1497
Size: 658 248 1022 979 1814
Fragments arranged by size:
1814 1022 979 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 163 177 195 358 390 1070 1102 1444
Size: 18 14 18 163 32 680 32 342
Cuts at: 1444 1450 1476 1482 1630 1662 2563 2593 2595
Size: 6 26 6 148 32 901 30 2
Cuts at: 2595 2625 145
Size: 30 2241
Fragments arranged by size:
2241 901 680 342 163 148 32 32
32 30 30 26 18 18 14 6
6 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
HindIII A'AGCT_T
Cuts at: 208 208
Size: 4721
KasI G'GCGC_C
Cuts at: 1493 2151 2399 1493
Size: 658 248 3815
Fragments arranged by size:
3815 658 248
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 131 224 833 1237 3392 3576 131
Size: 93 609 404 2155 184 1276
Fragments arranged by size:
2155 1276 609 404 184 93
MscI TGG'CCA
Cuts at: 871 1105 2482 871
Size: 234 1377 3110
Fragments arranged by size:
3110 1377 234
MslI CAynn'nnrTG
Cuts at: 541 835 899 1093 1111 1354 2710 2837 541
Size: 294 64 194 18 243 1356 127 2425
Fragments arranged by size:
2425 1356 294 243 194 127 64 18
MspA1I CmG'CkG
Cuts at: 1164 1832 2146 2506 3519 3764 1164
Size: 668 314 360 1013 245 2121
Fragments arranged by size:
2121 1013 668 360 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 1494 2152 2400 1494
Size: 658 248 3815
Fragments arranged by size:
3815 658 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4721
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NspI r_CATG'y
Cuts at: 1116 1774 2274 3181 1116
Size: 658 500 907 2656
Fragments arranged by size:
2656 907 658 500
PciI A'CATG_T
Cuts at: 1112 3177 1112
Size: 2065 2656
PflMI CCAn_nnn'nTGG
Cuts at: 1238 1238
Size: 4721
PfoI T'CCnGG_A
Cuts at: 728 728
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PspOMI G'GGCC_C
Cuts at: 546 546
Size: 4721
PstI C_TGCA'G
Cuts at: 365 888 1768 2453 365
Size: 523 880 685 2633
Fragments arranged by size:
2633 880 685 523
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 1164 2146 2506 1164
Size: 982 360 3379
Fragments arranged by size:
3379 982 360
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 866 1735 866
Size: 869 3852
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SfcI C'TryA_G
Cuts at: 33 361 884 1764 2449 3442 3633 33
Size: 328 523 880 685 993 191 1121
Fragments arranged by size:
1121 993 880 685 523 328 191
SfoI GGC'GCC
Cuts at: 1495 2153 2401 1495
Size: 658 248 3815
Fragments arranged by size:
3815 658 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 436 1743 436
Size: 1307 3414
SmlI C'TyrA_G
Cuts at: 580 1876 2791 3283 3545 580
Size: 1296 915 492 262 1756
Fragments arranged by size:
1756 1296 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
SrfI GCCC'GGGC
Cuts at: 436 436
Size: 4721
StyI C'CwwG_G
Cuts at: 107 737 1055 1184 1262 1855 2209 107
Size: 630 318 129 78 593 354 2619
Fragments arranged by size:
2619 630 593 354 318 129 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 203 439 1241 2304 2437 2896 3105 203
Size: 236 802 1063 133 459 209 1819
Fragments arranged by size:
1819 1063 802 459 236 209 133
TatI w'GTAC_w
Cuts at: 290 758 962 4236 290
Size: 468 204 3274 775
Fragments arranged by size:
3274 775 468 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 4238 4270 583
Size: 32 3623 32 1034
Fragments arranged by size:
3623 1034 32 32
Tth111I GACn'n_nGTC
Cuts at: 942 2518 942
Size: 1576 3145
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1235 1239 1269 1536 2216 1235
Size: 4 30 267 680 3740
Fragments arranged by size:
3740 680 267 30 4
XmaI C'CCGG_G
Cuts at: 434 1741 434
Size: 1307 3414
XmnI GAAnn'nnTTC
Cuts at: 1039 1039
Size: 4721
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
ZraI