Restriction map for
pF1KE1498
(Circular) (Six-base) MAPSORT of: pF1KE1498.seq Check: 6406 from: 1 to: 7886
pF1KE1498 7886 bp
With 182 enzymes: SgfI *
July 15, 2010 17:11 ..
AccI GT'mk_AC
Cuts at: 443 2040 4924 443
Size: 1597 2884 3405
Fragments arranged by size:
3405 2884 1597
Acc65I G'GTAC_C
Cuts at: 346 3432 4902 346
Size: 3086 1470 3330
Fragments arranged by size:
3330 3086 1470
AclI AA'CG_TT
Cuts at: 2351 7726 2351
Size: 5375 2511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 959 1022 1859 1975 4077 4223 4402 4675 5711
Size: 63 837 116 2102 146 179 273 1036
Cuts at: 5711 6143 6890 7158 959
Size: 432 747 268 1687
Fragments arranged by size:
2102 1687 1036 837 747 432 273 268
179 146 116 63
AfeI AGC'GCT
Cuts at: 4124 4124
Size: 7886
AflIII A'CryG_T
Cuts at: 129 4745 5238 6342 7319 129
Size: 4616 493 1104 977 696
Fragments arranged by size:
4616 1104 977 696 493
AgeI A'CCGG_T
Cuts at: 2749 6274 2749
Size: 3525 4361
AhdI GACnn_n'nnGTC
Cuts at: 1143 1551 1143
Size: 408 7478
AleI CACnn'nnGTG
Cuts at: 3271 3922 3271
Size: 651 7235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1057 1057
Size: 7886
AlwNI CAG_nnn'CTG
Cuts at: 309 472 493 782 934 1882 2671 4417 5191
Size: 163 21 289 152 948 789 1746 774
Cuts at: 5191 6758 309
Size: 1567 1437
Fragments arranged by size:
1746 1567 1437 948 789 774 289 163
152 21
ApaI G_GGCC'C
Cuts at: 578 578
Size: 7886
ApaLI G'TGCA_C
Cuts at: 1447 4376 6656 1447
Size: 2929 2280 2677
Fragments arranged by size:
2929 2677 2280
ApoI r'AATT_y
Cuts at: 652 862 882 2536 3366 3397 4890 7239 7495
Size: 210 20 1654 830 31 1493 2349 256
Cuts at: 7495 652
Size: 1043
Fragments arranged by size:
2349 1654 1493 1043 830 256 210 31
20
AseI AT'TA_AT
Cuts at: 20 20
Size: 7886
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
AvaI C'yCGr_G
Cuts at: 237 740 1939 2578 2890 4906 237
Size: 503 1199 639 312 2016 3217
Fragments arranged by size:
3217 2016 1199 639 503 312
BamHI G'GATC_C
Cuts at: 3683 4911 3683
Size: 1228 6658
BanI G'GyrC_C
Cuts at: 346 1006 1351 1420 1620 1763 3432 4657 4902
Size: 660 345 69 200 143 1669 1225 245
Cuts at: 4902 5316 5564 5599 346
Size: 414 248 35 2633
Fragments arranged by size:
2633 1669 1225 660 414 345 248 245
200 143 69 35
BanII G_rGCy'C
Cuts at: 332 370 578 1363 2968 3163 3251 4900 5930
Size: 38 208 785 1605 195 88 1649 1030
Cuts at: 5930 332
Size: 2288
Fragments arranged by size:
2288 1649 1605 1030 785 208 195 88
38
BbeI G_GCGC'C
Cuts at: 1424 5320 5568 1424
Size: 3896 248 3742
Fragments arranged by size:
3896 3742 248
BbsI GAAGACnn'nnnn_
Cuts at: 362 1641 1784 1865 2142 2591 3984 4311 4850
Size: 1279 143 81 277 449 1393 327 539
Cuts at: 4850 362
Size: 3398
Fragments arranged by size:
3398 1393 1279 539 449 327 277 143
81
BbvCI CC'TCA_GC
Cuts at: 536 1657 3188 536
Size: 1121 1531 5234
Fragments arranged by size:
5234 1531 1121
BciVI GTATCCnnnnn_n'
Cuts at: 2199 5784 6545 7886 2199
Size: 3585 761 1341 2199
Fragments arranged by size:
3585 2199 1341 761
BclI T'GATC_A
Cuts at: 3084 3462 5406 3084
Size: 378 1944 5564
Fragments arranged by size:
5564 1944 378
BglI GCCn_nnn'nGGC
Cuts at: 1324 2104 4396 1324
Size: 780 2292 4814
Fragments arranged by size:
4814 2292 780
BglII A'GATC_T
Cuts at: 1916 3888 5401 7438 1916
Size: 1972 1513 2037 2364
Fragments arranged by size:
2364 2037 1972 1513
BlpI GC'TnA_GC
Cuts at: 775 4998 775
Size: 4223 3663
Bme1580I G_kGCm'C
Cuts at: 578 1405 1451 2999 4380 5511 5604 6660 578
Size: 827 46 1548 1381 1131 93 1056 1804
Fragments arranged by size:
1804 1548 1381 1131 1056 827 93 46
BmgBI CAC'GTC
Cuts at: 3354 3762 3354
Size: 408 7478
BmrI ACTGGGnnnn_n'
Cuts at: 297 1512 4092 5098 5179 5514 7658 297
Size: 1215 2580 1006 81 335 2144 525
Fragments arranged by size:
2580 2144 1215 1006 525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 351 1338 3182 6253 351
Size: 987 1844 3071 1984
Fragments arranged by size:
3071 1984 1844 987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 851 1155 1300 1330 2147 2500 2917 3023
Size: 505 304 145 30 817 353 417 106
Cuts at: 3023 3274 3673 4825 4842 346
Size: 251 399 1152 17 3390
Fragments arranged by size:
3390 1152 817 505 417 399 353 304
251 145 106 30 17
Bpu10I CC'TnA_GC
Cuts at: 536 1357 1625 1657 3188 3706 536
Size: 821 268 32 1531 518 4716
Fragments arranged by size:
4716 1531 821 518 268 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 2815 3472 3673 4750 5062 5941 6433 6731
Size: 2395 657 201 1077 312 879 492 298
Cuts at: 6731 420
Size: 1575
Fragments arranged by size:
2395 1575 1077 879 657 492 312 298
201
BsaI GGTCTCn'nnnn_
Cuts at: 396 3025 3040 3896 4667 7590 396
Size: 2629 15 856 771 2923 692
Fragments arranged by size:
2923 2629 856 771 692 15
BsaAI yAC'GTr
Cuts at: 2730 3273 5239 2730
Size: 543 1966 5377
Fragments arranged by size:
5377 1966 543
BsaBI GATnn'nnATC
Cuts at: 6 5400 7437 6
Size: 5394 2037 455
Fragments arranged by size:
5394 2037 455
BsaHI Gr'CG_yC
Cuts at: 1421 2819 5317 5565 6267 7763 1421
Size: 1398 2498 248 702 1496 1544
Fragments arranged by size:
2498 1544 1496 1398 702 248
BsaWI w'CCGG_w
Cuts at: 2749 5075 5596 6274 6548 6695 7106 2749
Size: 2326 521 678 274 147 411 3529
Fragments arranged by size:
3529 2326 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 4501 4531 244
Size: 30 4227 30 3599
Fragments arranged by size:
4227 3599 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 748 938 1327 3058 3158 3757 4303 4455
Size: 237 190 389 1731 100 599 546 152
Cuts at: 4455 4458 4576 4603 511
Size: 3 118 27 3794
Fragments arranged by size:
3794 1731 599 546 389 237 190 152
118 100 27 3
BseYI C'CCAG_C
Cuts at: 292 436 2865 3951 4340 4520 5311 6069 6646
Size: 144 2429 1086 389 180 791 758 577
Cuts at: 6646 7142 292
Size: 496 1036
Fragments arranged by size:
2429 1086 1036 791 758 577 496 389
180 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 1633 2450 4169 496
Size: 1137 817 1719 4213
Fragments arranged by size:
4213 1719 1137 817
BsiEI CG_ry'CG
Cuts at: 104 1757 2447 5428 5474 6682 104
Size: 1653 690 2981 46 1208 1308
Fragments arranged by size:
2981 1653 1308 1208 690 46
BsiHKAI G_wGCw'C
Cuts at: 140 274 1291 1363 1451 1631 3251 3749 4380
Size: 134 1017 72 88 180 1620 498 631
Cuts at: 4380 4900 5678 5868 6660 140
Size: 520 778 190 792 1366
Fragments arranged by size:
1620 1366 1017 792 778 631 520 498
190 180 134 88 72
BsmI GAATG_Cn'
Cuts at: 555 3607 3985 4278 555
Size: 3052 378 293 4163
Fragments arranged by size:
4163 3052 378 293
BsmBI CGTCTCn'nnnn_
Cuts at: 583 3250 4497 583
Size: 2667 1247 3972
Fragments arranged by size:
3972 2667 1247
Bsp1286I G_dGCh'C
Cuts at: 140 274 332 370 578 1291 1363 1405 1451
Size: 134 58 38 208 713 72 42 46
Cuts at: 1451 1631 2968 2999 3163 3251 3749 4380 4900
Size: 180 1337 31 164 88 498 631 520
Cuts at: 4900 5511 5604 5678 5868 5930 6660 140
Size: 611 93 74 190 62 730 1366
Fragments arranged by size:
1366 1337 730 713 631 611 520 498
208 190 180 164 134 93 88 74
72 62 58 46 42 38 31
BspEI T'CCGG_A
Cuts at: 7106 7106
Size: 7886
BspHI T'CATG_A
Cuts at: 7062 7062
Size: 7886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1541 2171 2534 3914 4119 4685 4936 5150 5452
Size: 630 363 1380 205 566 251 214 302
Cuts at: 5452 5833 1541
Size: 381 3594
Fragments arranged by size:
3594 1380 630 566 381 363 302 251
214 205
BsrBI CCG'CTC
Cuts at: 50 191 3037 4686 6178 6232 7716 7881 50
Size: 141 2846 1649 1492 54 1484 165 55
Fragments arranged by size:
2846 1649 1492 1484 165 141 55 54
BsrDI GCAATG_nn'
Cuts at: 5798 5798
Size: 7886
BsrFI r'CCGG_y
Cuts at: 1318 1417 2749 3942 4395 5884 6065 6274 1318
Size: 99 1332 1193 453 1489 181 209 2930
Fragments arranged by size:
2930 1489 1332 1193 453 209 181 99
BsrGI T'GTAC_A
Cuts at: 2603 2603
Size: 7886
BssSI C'ACGA_G
Cuts at: 139 2389 6157 6515 139
Size: 2250 3768 358 1510
Fragments arranged by size:
3768 2250 1510 358
BstAPI GCAn_nnn'nTGC
Cuts at: 483 1620 4417 5263 483
Size: 1137 2797 846 3106
Fragments arranged by size:
3106 2797 1137 846
BstBI TT'CG_AA
Cuts at: 2718 6247 2718
Size: 3529 4357
BstXI CCAn_nnnn'nTGG
Cuts at: 378 585 801 3573 378
Size: 207 216 2772 4691
Fragments arranged by size:
4691 2772 216 207
BstYI r'GATC_y
Cuts at: 1 1916 2158 3683 3888 4054 4911 5378 5401
Size: 1915 242 1525 205 166 857 467 23
Cuts at: 5401 5736 5982 6994 7080 7092 7438 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1915 1525 1012 857 467 449 346 335
246 242 205 166 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3170 3170
Size: 7886
BtgI C'CryG_G
Cuts at: 107 766 2057 3179 4115 107
Size: 659 1291 1122 936 3878
Fragments arranged by size:
3878 1291 1122 936 659
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1349 5836 6017 6180 1349
Size: 4487 181 163 3055
Fragments arranged by size:
4487 3055 181 163
BtsI GCAGTG_nn'
Cuts at: 1098 1748 2462 3030 4418 5129 1098
Size: 650 714 568 1388 711 3855
Fragments arranged by size:
3855 1388 714 711 650 568
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2033 2068 2033
Size: 35 7851
DraI TTT'AAA
Cuts at: 3284 4885 3284
Size: 1601 6285
DraIII CAC_nnn'GTG
Cuts at: 2937 2937
Size: 7886
DrdI GACnn_nn'nnGTC
Cuts at: 427 2383 5592 6450 427
Size: 1956 3209 858 1863
Fragments arranged by size:
3209 1956 1863 858
EaeI y'GGCC_r
Cuts at: 1427 2054 2233 2452 3113 3764 4397 5471 5645
Size: 627 179 219 661 651 633 1074 174
Cuts at: 5645 6036 6063 7408 1427
Size: 391 27 1345 1905
Fragments arranged by size:
1905 1345 1074 661 651 633 627 391
219 179 174 27
EagI C'GGCC_G
Cuts at: 5471 5471
Size: 7886
EarI CTCTTCn'nnn_
Cuts at: 429 713 935 1047 2835 3320 3818 5909 6119
Size: 284 222 112 1788 485 498 2091 210
Cuts at: 6119 429
Size: 2196
Fragments arranged by size:
2196 2091 1788 498 485 284 222 210
112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 702 6094 6404 6550 7696 702
Size: 5392 310 146 1146 892
Fragments arranged by size:
5392 1146 892 310 146
EcoICRI GAG'CTC
Cuts at: 1361 3249 4898 1361
Size: 1888 1649 4349
Fragments arranged by size:
4349 1888 1649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 851 959 1022 1155 1300 1330 1859 1975
Size: 505 108 63 133 145 30 529 116
Cuts at: 1975 2147 2500 2917 3023 3274 3673 4077 4223
Size: 172 353 417 106 251 399 404 146
Cuts at: 4223 4402 4675 4825 4842 5711 6143 6890 7158
Size: 179 273 150 17 869 432 747 268
Cuts at: 7158 346
Size: 1074
Fragments arranged by size:
1074 869 747 529 505 432 417 404
399 353 273 268 251 179 172 150
146 145 133 116 108 106 63 30
17
EcoNI CCTnn'n_nnAGG
Cuts at: 1312 4358 1312
Size: 3046 4840
EcoO109I rG'GnC_Cy
Cuts at: 2882 3184 3570 4086 5025 2882
Size: 302 386 516 939 5743
Fragments arranged by size:
5743 939 516 386 302
EcoRI G'AATT_C
Cuts at: 652 3397 4890 652
Size: 2745 1493 3648
Fragments arranged by size:
3648 2745 1493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1659 3627 5905 1659
Size: 1968 2278 3640
Fragments arranged by size:
3640 2278 1968
FseI GG_CCGG'CC
Cuts at: 4399 4399
Size: 7886
FspI TGC'GCA
Cuts at: 1411 3232 5667 1411
Size: 1821 2435 3630
Fragments arranged by size:
3630 2435 1821
HaeII r_GCGC'y
Cuts at: 1424 4126 5320 5568 6590 7569 1424
Size: 2702 1194 248 1022 979 1741
Fragments arranged by size:
2702 1741 1194 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 1537 1569 2197 2229 2790 2822 2836
Size: 32 376 32 628 32 561 32 14
Cuts at: 2836 2868 3052 3084 4094 4126 4298 4330 4477
Size: 32 184 32 1010 32 172 32 147
Cuts at: 4477 4501 4509 4533 5728 5758 5760 5790 1129
Size: 24 8 24 1195 30 2 30 3225
Fragments arranged by size:
3225 1195 1010 628 561 376 184 172
147 32 32 32 32 32 32 32
32 30 30 24 24 14 8 2
HincII GTy'rAC
Cuts at: 127 2041 4925 127
Size: 1914 2884 3088
Fragments arranged by size:
3088 2884 1914
HindIII A'AGCT_T
Cuts at: 3448 3448
Size: 7886
KasI G'GCGC_C
Cuts at: 1420 5316 5564 1420
Size: 3896 248 3742
Fragments arranged by size:
3896 3742 248
KpnI G_GTAC'C
Cuts at: 350 3436 4906 350
Size: 3086 1470 3330
Fragments arranged by size:
3330 3086 1470
MluI A'CGCG_T
Cuts at: 4745 7319 4745
Size: 2574 5312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 859 1355 1812 6557 6741 859
Size: 496 457 4745 184 2004
Fragments arranged by size:
4745 2004 496 457 184
MscI TGG'CCA
Cuts at: 2056 3766 5647 2056
Size: 1710 1881 4295
Fragments arranged by size:
4295 1881 1710
MslI CAynn'nnrTG
Cuts at: 376 583 1334 3271 3800 3922 5875 6002 376
Size: 207 751 1937 529 122 1953 127 2260
Fragments arranged by size:
2260 1953 1937 751 529 207 127 122
MspA1I CmG'CkG
Cuts at: 768 931 1217 1349 1869 1973 2668 3192 3849
Size: 163 286 132 520 104 695 524 657
Cuts at: 3849 4713 4997 5311 5671 6684 6929 768
Size: 864 284 314 360 1013 245 1725
Fragments arranged by size:
1725 1013 864 695 657 524 520 360
314 286 284 245 163 132 104
NaeI GCC'GGC
Cuts at: 1320 1419 3944 4397 6067 1320
Size: 99 2525 453 1670 3139
Fragments arranged by size:
3139 2525 1670 453 99
NarI GG'CG_CC
Cuts at: 1421 5317 5565 1421
Size: 3896 248 3742
Fragments arranged by size:
3896 3742 248
NcoI C'CATG_G
Cuts at: 107 2057 107
Size: 1950 5936
NgoMIV G'CCGG_C
Cuts at: 1318 1417 3942 4395 6065 1318
Size: 99 2525 453 1670 3139
Fragments arranged by size:
3139 2525 1670 453 99
NruI TCG'CGA
Cuts at: 1759 1759
Size: 7886
NspI r_CATG'y
Cuts at: 133 163 486 1044 4939 5439 6346 133
Size: 30 323 558 3895 500 907 1673
Fragments arranged by size:
3895 1673 907 558 500 323 30
PasI CC'CwG_GG
Cuts at: 1668 2992 1668
Size: 1324 6562
PciI A'CATG_T
Cuts at: 129 6342 129
Size: 6213 1673
PflMI CCAn_nnn'nTGG
Cuts at: 309 2937 309
Size: 2628 5258
PfoI T'CCnGG_A
Cuts at: 3251 4858 3251
Size: 1607 6279
PmeI GTTT'AAAC
Cuts at: 4885 4885
Size: 7886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7025 7057 7025
Size: 32 7854
PpuMI rG'GwC_Cy
Cuts at: 2882 3570 4086 2882
Size: 688 516 6682
Fragments arranged by size:
6682 688 516
PshAI GACnn'nnGTC
Cuts at: 408 2446 408
Size: 2038 5848
PsiI TTA'TAA
Cuts at: 965 965
Size: 7886
PspOMI G'GGCC_C
Cuts at: 574 574
Size: 7886
PstI C_TGCA'G
Cuts at: 627 4130 4240 4682 4933 5618 627
Size: 3503 110 442 251 685 2895
Fragments arranged by size:
3503 2895 685 442 251 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7886
PvuII CAG'CTG
Cuts at: 931 1869 2668 3192 3849 5311 5671 931
Size: 938 799 524 657 1462 360 3146
Fragments arranged by size:
3146 1462 938 799 657 524 360
RsrII CG'GwC_CG
Cuts at: 6081 6081
Size: 7886
SacI G_AGCT'C
Cuts at: 1363 3251 4900 1363
Size: 1888 1649 4349
Fragments arranged by size:
4349 1888 1649
SacII CC_GC'GG
Cuts at: 769 769
Size: 7886
SalI G'TCGA_C
Cuts at: 2039 4923 2039
Size: 2884 5002
SanDI GG'GwC_CC
Cuts at: 4086 4086
Size: 7886
SapI GCTCTTCn'nnn_
Cuts at: 1047 5909 6119 1047
Size: 4862 210 2814
Fragments arranged by size:
4862 2814 210
SbfI CC_TGCA'GG
Cuts at: 4682 4933 4682
Size: 251 7635
ScaI AGT'ACT
Cuts at: 7403 7403
Size: 7886
SfcI C'TryA_G
Cuts at: 33 623 978 3531 4126 4236 4678 4929 5614
Size: 590 355 2553 595 110 442 251 685
Cuts at: 5614 6607 6798 33
Size: 993 191 1121
Fragments arranged by size:
2553 1121 993 685 595 590 442 355
251 191 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 4396 4396
Size: 7886
SfoI GGC'GCC
Cuts at: 1422 5318 5566 1422
Size: 3896 248 3742
Fragments arranged by size:
3896 3742 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
SmaI CCC'GGG
Cuts at: 4908 4908
Size: 7886
SmlI C'TyrA_G
Cuts at: 399 1939 2794 3451 3688 4765 5041 5956 6448
Size: 1540 855 657 237 1077 276 915 492
Cuts at: 6448 6710 399
Size: 262 1575
Fragments arranged by size:
1575 1540 1077 915 855 657 492 276
262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7886
SphI G_CATG'C
Cuts at: 163 486 1044 4939 5439 163
Size: 323 558 3895 500 2610
Fragments arranged by size:
3895 2610 558 500 323
SspI AAT'ATT
Cuts at: 1907 2297 1907
Size: 390 7496
StuI AGG'CCT
Cuts at: 2771 4802 2771
Size: 2031 5855
StyI C'CwwG_G
Cuts at: 107 800 2057 2067 2955 3566 5020 5374 107
Size: 693 1257 10 888 611 1454 354 2619
Fragments arranged by size:
2619 1454 1257 888 693 611 354 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 398 793 1197 1494 1584 1660 1846 3064
Size: 32 395 404 297 90 76 186 1218
Cuts at: 3064 3352 3742 4565 5469 5602 6061 6270 366
Size: 288 390 823 904 133 459 209 1982
Fragments arranged by size:
1982 1218 904 823 459 404 395 390
297 288 209 186 133 90 76 32
TatI w'GTAC_w
Cuts at: 2603 3100 7401 2603
Size: 497 4301 3088
Fragments arranged by size:
4301 3088 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 3546 3578 4051 4083 7403 7435 244
Size: 32 3270 32 473 32 3320 32 695
Fragments arranged by size:
3320 3270 695 473 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1645 5683 1645
Size: 4038 3848
XbaI T'CTAG_A
Cuts at: 3411 4917 3411
Size: 1506 6380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3826 5381 3826
Size: 1555 6331
XhoI C'TCGA_G
Cuts at: 1939 1939
Size: 7886
XmaI C'CCGG_G
Cuts at: 4906 4906
Size: 7886
XmnI GAAnn'nnTTC
Cuts at: 2076 3329 2076
Size: 1253 6633
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BssHII BstEII BstZ17I ClaI EcoRV
FspAI HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PspXI PsrI SexAI SgrAI SnaBI SrfI SwaI
ZraI