Restriction map for
pF1KE1499
(Circular) (Six-base) MAPSORT of: pF1KE1499.seq Check: 6306 from: 1 to: 12404
pF1KE1499 12404 bp
With 182 enzymes: SgfI *
August 2, 2010 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1827 2262 2849 2994 1827
Size: 435 587 145 11237
Fragments arranged by size:
11237 587 435 145
AatII G_ACGT'C
Cuts at: 2642 3356 5041 2642
Size: 714 1685 10005
Fragments arranged by size:
10005 1685 714
AccI GT'mk_AC
Cuts at: 1520 2723 3621 7823 9442 1520
Size: 1203 898 4202 1619 4482
Fragments arranged by size:
4482 4202 1619 1203 898
Acc65I G'GTAC_C
Cuts at: 8819 9420 8819
Size: 601 11803
AclI AA'CG_TT
Cuts at: 373 12244 373
Size: 11871 533
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 802 916 940 1129 1231 3340 3839 4784
Size: 249 114 24 189 102 2109 499 945
Cuts at: 4784 4918 4930 6460 8359 8792 10229 10661 11408
Size: 134 12 1530 1899 433 1437 432 747
Cuts at: 11408 11676 553
Size: 268 1281
Fragments arranged by size:
2109 1899 1530 1437 1281 945 747 499
433 432 268 249 189 134 114 102
24 12
AfeI AGC'GCT
Cuts at: 2242 2242
Size: 12404
AflII C'TTAA_G
Cuts at: 4819 4819
Size: 12404
AflIII A'CryG_T
Cuts at: 1286 1442 2120 2198 4746 6275 7868 8191 8378
Size: 156 678 78 2548 1529 1593 323 187
Cuts at: 8378 9293 9756 10860 11837 1286
Size: 915 463 1104 977 1853
Fragments arranged by size:
2548 1853 1593 1529 1104 977 915 678
463 323 187 156 78
AgeI A'CCGG_T
Cuts at: 10792 10792
Size: 12404
AhdI GACnn_n'nnGTC
Cuts at: 2514 7203 2514
Size: 4689 7715
AleI CACnn'nnGTG
Cuts at: 2143 7798 7949 2143
Size: 5655 151 6598
Fragments arranged by size:
6598 5655 151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3761 5440 7558 9403 3761
Size: 1679 2118 1845 6762
Fragments arranged by size:
6762 2118 1845 1679
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7823 7855 8912 8944 8998 9030 7823
Size: 32 1057 32 54 32 11197
Fragments arranged by size:
11197 1057 54 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 951 1080 1687 1803 2397 2748 2865 7085 8964
Size: 129 607 116 594 351 117 4220 1879
Cuts at: 8964 8989 9255 9709 11276 951
Size: 25 266 454 1567 2079
Fragments arranged by size:
4220 2079 1879 1567 607 594 454 351
266 129 117 116 25
ApaI G_GGCC'C
Cuts at: 3253 5388 5404 7047 7229 8269 3253
Size: 2135 16 1643 182 1040 7388
Fragments arranged by size:
7388 2135 1643 1040 182 16
ApaLI G'TGCA_C
Cuts at: 1708 11174 1708
Size: 9466 2938
ApoI r'AATT_y
Cuts at: 634 640 4153 4768 6811 6911 7844 8164 8977
Size: 6 3513 615 2043 100 933 320 813
Cuts at: 8977 9408 11757 12013 634
Size: 431 2349 256 1025
Fragments arranged by size:
3513 2349 2043 1025 933 813 615 431
320 256 100 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 12404
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12404
AvaI C'yCGr_G
Cuts at: 183 2512 2735 3187 5655 7179 7222 7255 7383
Size: 2329 223 452 2468 1524 43 33 128
Cuts at: 7383 7454 7614 9001 9424 183
Size: 71 160 1387 423 3163
Fragments arranged by size:
3163 2468 2329 1524 1387 452 423 223
160 128 71 43 33
AvrII C'CTAG_G
Cuts at: 8436 8436
Size: 12404
BamHI G'GATC_C
Cuts at: 2873 3566 6241 6893 7127 9429 2873
Size: 693 2675 652 234 2302 5848
Fragments arranged by size:
5848 2675 2302 693 652 234
BanI G'GyrC_C
Cuts at: 199 7093 7369 7432 8819 9420 9834 10082 10117
Size: 6894 276 63 1387 601 414 248 35
Cuts at: 10117 199
Size: 2486
Fragments arranged by size:
6894 2486 1387 601 414 276 248 63
35
BanII G_rGCy'C
Cuts at: 867 2429 2459 3253 3616 3641 4007 4908 4919
Size: 1562 30 794 363 25 366 901 11
Cuts at: 4919 5388 5395 5404 5834 7047 7229 7360 7396
Size: 469 7 9 430 1213 182 131 36
Cuts at: 7396 7523 7558 7570 8269 9418 10448 867
Size: 127 35 12 699 1149 1030 2823
Fragments arranged by size:
2823 1562 1213 1149 1030 901 794 699
469 430 366 363 182 131 127 36
35 30 25 12 11 9 7
BbeI G_GCGC'C
Cuts at: 7097 7373 9838 10086 7097
Size: 276 2465 248 9415
Fragments arranged by size:
9415 2465 276 248
BbsI GAAGACnn'nnnn_
Cuts at: 776 2072 3227 3348 6021 776
Size: 1296 1155 121 2673 7159
Fragments arranged by size:
7159 2673 1296 1155 121
BbvCI CC'TCA_GC
Cuts at: 1132 4516 7913 1132
Size: 3384 3397 5623
Fragments arranged by size:
5623 3397 3384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2185 2219 3168 3202 5551 5585 2185
Size: 34 949 34 2349 34 9004
Fragments arranged by size:
9004 2349 949 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 956 3919 4047 6471 7665 9021 10302 11063 12404
Size: 2963 128 2424 1194 1356 1281 761 1341
Cuts at: 12404 956
Size: 956
Fragments arranged by size:
2963 2424 1356 1341 1281 1194 956 761
128
BclI T'GATC_A
Cuts at: 578 2288 3284 6398 8547 9924 578
Size: 1710 996 3114 2149 1377 3058
Fragments arranged by size:
3114 3058 2149 1710 1377 996
BglI GCCn_nnn'nGGC
Cuts at: 166 175 3553 3748 7070 7258 7281 7598 166
Size: 9 3378 195 3322 188 23 317 4972
Fragments arranged by size:
4972 3378 3322 317 195 188 23 9
BglII A'GATC_T
Cuts at: 2565 2658 6390 9919 11956 2565
Size: 93 3732 3529 2037 3013
Fragments arranged by size:
3732 3529 3013 2037 93
BlpI GC'TnA_GC
Cuts at: 4001 4480 5458 8507 9516 4001
Size: 479 978 3049 1009 6889
Fragments arranged by size:
6889 3049 1009 978 479
Bme1580I G_kGCm'C
Cuts at: 1712 3253 5388 5404 5483 5711 7047 7229 7435
Size: 1541 2135 16 79 228 1336 182 206
Cuts at: 7435 7906 8269 10029 10122 11178 1712
Size: 471 363 1760 93 1056 2938
Fragments arranged by size:
2938 2135 1760 1541 1336 1056 471 363
228 206 182 93 79 16
BmgBI CAC'GTC
Cuts at: 2162 2199 4430 6840 9296 2162
Size: 37 2231 2410 2456 5270
Fragments arranged by size:
5270 2456 2410 2231 37
BmrI ACTGGGnnnn_n'
Cuts at: 2027 7043 8974 9616 9697 10032 12176 2027
Size: 5016 1931 642 81 335 2144 2255
Fragments arranged by size:
5016 2255 2144 1931 642 335 81
BmtI G_CTAG'C
Cuts at: 7427 7427
Size: 12404
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10771 10771
Size: 12404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1405 1627 1780 2188 2322 2473 2575 2773
Size: 576 222 153 408 134 151 102 198
Cuts at: 2773 2836 2929 3319 3600 3868 4489 5185 5253
Size: 63 93 390 281 268 621 696 68
Cuts at: 5253 5310 5350 5734 5896 6802 7372 7476 7584
Size: 57 40 384 162 906 570 104 108
Cuts at: 7584 7618 8632 9226 829
Size: 34 1014 594 4007
Fragments arranged by size:
4007 1014 906 696 621 594 576 570
408 390 384 281 268 222 198 162
153 151 134 108 104 102 93 68
63 57 40 34
Bpu10I CC'TnA_GC
Cuts at: 1002 1132 2320 4516 5623 7913 1002
Size: 130 1188 2196 1107 2290 5493
Fragments arranged by size:
5493 2290 2196 1188 1107 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 3112 6436 7969 9580 10459 10951 11249 3094
Size: 18 3324 1533 1611 879 492 298 4249
Fragments arranged by size:
4249 3324 1611 1533 879 492 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 663 4028 4751 6841 7170 8200 8680 9097 12108
Size: 3365 723 2090 329 1030 480 417 3011
Cuts at: 12108 663
Size: 959
Fragments arranged by size:
3365 3011 2090 1030 959 723 480 417
329
BsaAI yAC'GTr
Cuts at: 3273 3528 4058 5484 7691 8192 8381 9032 9057
Size: 255 530 1426 2207 501 189 651 25
Cuts at: 9057 9757 3273
Size: 700 5920
Fragments arranged by size:
5920 2207 1426 700 651 530 501 255
189 25
BsaBI GATnn'nnATC
Cuts at: 6 751 5023 9918 11955 6
Size: 745 4272 4895 2037 455
Fragments arranged by size:
4895 4272 2037 745 455
BsaHI Gr'CG_yC
Cuts at: 2489 2639 3085 3277 3353 5038 5336 5540 5702
Size: 150 446 192 76 1685 298 204 162
Cuts at: 5702 7094 7301 7370 7400 9284 9835 10083 10785
Size: 1392 207 69 30 1884 551 248 702
Cuts at: 10785 12281 2489
Size: 1496 2612
Fragments arranged by size:
2612 1884 1685 1496 1392 702 551 446
298 248 207 204 192 162 150 76
69 30
BsaWI w'CCGG_w
Cuts at: 2069 8816 9593 10114 10792 11066 11213 11624 2069
Size: 6747 777 521 678 274 147 411 2849
Fragments arranged by size:
6747 2849 777 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1378 1408 1432 1462 2347 2377 2692 2722 5302
Size: 30 24 30 885 30 315 30 2580
Cuts at: 5302 5332 7612 7642 7823 7853 8293 8323 8470
Size: 30 2280 30 181 30 440 30 147
Cuts at: 8470 8500 8912 8942 8998 9028 9172 9202 1378
Size: 30 412 30 56 30 144 30 4580
Fragments arranged by size:
4580 2580 2280 885 440 412 315 181
147 144 56 30 30 30 30 30
30 30 30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 472 2185 2437 3555 3670 5614 5640 5643
Size: 240 1713 252 1118 115 1944 26 3
Cuts at: 5643 5704 5737 5955 7158 7686 8662 232
Size: 61 33 218 1203 528 976 3974
Fragments arranged by size:
3974 1944 1713 1203 1118 976 528 252
240 218 115 61 33 26 3
BseYI C'CCAG_C
Cuts at: 451 2865 3246 5220 5887 7078 7099 7133 7267
Size: 2414 381 1974 667 1191 21 34 134
Cuts at: 7267 7276 7531 9829 10587 11164 11660 451
Size: 9 255 2298 758 577 496 1195
Fragments arranged by size:
2414 2298 1974 1195 1191 758 667 577
496 381 255 134 34 21 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1066 1726 2245 2848 3028 3355 5843 6430
Size: 21 660 519 603 180 327 2488 587
Cuts at: 6430 7738 7973 8809 1045
Size: 1308 235 836 4640
Fragments arranged by size:
4640 2488 1308 836 660 603 587 519
327 235 180 21
BsiEI CG_ry'CG
Cuts at: 104 5173 5413 5597 9946 9992 11200 104
Size: 5069 240 184 4349 46 1208 1308
Fragments arranged by size:
5069 4349 1308 1208 240 184 46
BsiHKAI G_wGCw'C
Cuts at: 1432 1712 2429 2459 2957 3641 3653 4007 5395
Size: 280 717 30 498 684 12 354 1388
Cuts at: 5395 5834 6317 7570 7690 7696 9050 9418 10196
Size: 439 483 1253 120 6 1354 368 778
Cuts at: 10196 10386 11178 1432
Size: 190 792 2658
Fragments arranged by size:
2658 1388 1354 1253 792 778 717 684
498 483 439 368 354 280 190 120
30 12 6
BsmI GAATG_Cn'
Cuts at: 3313 3351 6861 3313
Size: 38 3510 8856
Fragments arranged by size:
8856 3510 38
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 3092 3256 3495 4988 5045 5328 5584 2374
Size: 718 164 239 1493 57 283 256 9194
Fragments arranged by size:
9194 1493 718 283 256 239 164 57
Bsp1286I G_dGCh'C
Cuts at: 867 1432 1712 2429 2459 2957 3253 3616 3641
Size: 565 280 717 30 498 296 363 25
Cuts at: 3641 3653 4007 4908 4919 5388 5395 5404 5483
Size: 12 354 901 11 469 7 9 79
Cuts at: 5483 5711 5834 6317 7047 7229 7360 7396 7435
Size: 228 123 483 730 182 131 36 39
Cuts at: 7435 7523 7558 7570 7690 7696 7906 8269 9050
Size: 88 35 12 120 6 210 363 781
Cuts at: 9050 9418 10029 10122 10196 10386 10448 11178 867
Size: 368 611 93 74 190 62 730 2093
Fragments arranged by size:
2093 901 781 730 730 717 611 565
498 483 469 368 363 363 354 296
280 228 210 190 182 131 123 120
93 88 79 74 62 39 36 35
30 25 12 12 11 9 7 6
BspEI T'CCGG_A
Cuts at: 11624 11624
Size: 12404
BspHI T'CATG_A
Cuts at: 393 1935 2763 3945 6419 11580 393
Size: 1542 828 1182 2474 5161 1217
Fragments arranged by size:
5161 2474 1542 1217 1182 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1025 1827 2262 2756 2849 2994 3162 3377 8831
Size: 802 435 494 93 145 168 215 5454
Cuts at: 8831 9115 9454 9668 9970 10351 1025
Size: 284 339 214 302 381 3078
Fragments arranged by size:
5454 3078 802 494 435 381 339 302
284 215 214 168 145 93
BsrBI CCG'CTC
Cuts at: 50 113 1425 4884 4939 5169 5216 7488 9321
Size: 63 1312 3459 55 230 47 2272 1833
Cuts at: 9321 10696 10750 12234 12399 50
Size: 1375 54 1484 165 55
Fragments arranged by size:
3459 2272 1833 1484 1375 1312 230 165
63 55 55 54 47
BsrDI GCAATG_nn'
Cuts at: 789 1014 1921 5337 10316 789
Size: 225 907 3416 4979 2877
Fragments arranged by size:
4979 3416 2877 907 225
BsrFI r'CCGG_y
Cuts at: 202 1055 5542 7115 7261 9262 10402 10583 10792
Size: 853 4487 1573 146 2001 1140 181 209
Cuts at: 10792 202
Size: 1814
Fragments arranged by size:
4487 2001 1814 1573 1140 853 209 181
146
BsrGI T'GTAC_A
Cuts at: 6278 6278
Size: 12404
BssHII G'CGCG_C
Cuts at: 2996 7271 7417 7419 2996
Size: 4275 146 2 7981
Fragments arranged by size:
7981 4275 146 2
BssSI C'ACGA_G
Cuts at: 1732 2956 3652 5854 6259 7104 10675 11033 1732
Size: 1224 696 2202 405 845 3571 358 3103
Fragments arranged by size:
3571 3103 2202 1224 845 696 405 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1080 2826 7716 8658 9255 9781 1080
Size: 1746 4890 942 597 526 3703
Fragments arranged by size:
4890 3703 1746 942 597 526
BstBI TT'CG_AA
Cuts at: 270 4611 10765 270
Size: 4341 6154 1909
Fragments arranged by size:
6154 4341 1909
BstEII G'GTnAC_C
Cuts at: 8463 8616 8463
Size: 153 12251
BstXI CCAn_nnnn'nTGG
Cuts at: 234 1050 1329 2692 3577 4083 7776 8629 8907
Size: 816 279 1363 885 506 3693 853 278
Cuts at: 8907 234
Size: 3731
Fragments arranged by size:
3731 3693 1363 885 853 816 506 279
278
BstYI r'GATC_y
Cuts at: 1 414 536 2108 2565 2658 2873 3566 4977
Size: 413 122 1572 457 93 215 693 1411
Cuts at: 4977 6225 6241 6390 6893 7127 9429 9896 9919
Size: 1248 16 149 503 234 2302 467 23
Cuts at: 9919 10254 10500 11512 11598 11610 11956 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2302 1572 1411 1248 1012 693 503 467
457 449 413 346 335 246 234 215
149 122 93 86 23 16 12
BstZ17I GTA'TAC
Cuts at: 3622 3622
Size: 12404
Bsu36I CC'TnA_GG
Cuts at: 848 8428 848
Size: 7580 4824
BtgI C'CryG_G
Cuts at: 161 1238 1860 3608 5153 5354 5723 8357 161
Size: 1077 622 1748 1545 201 369 2634 4208
Fragments arranged by size:
4208 2634 1748 1545 1077 622 369 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 5010 5244 6935 8370 8769 10354 10535 10698
Size: 4782 234 1691 1435 399 1585 181 163
Cuts at: 10698 228
Size: 1934
Fragments arranged by size:
4782 1934 1691 1585 1435 399 234 181
163
BtsI GCAGTG_nn'
Cuts at: 978 2827 7805 8576 9256 9647 978
Size: 1849 4978 771 680 391 3735
Fragments arranged by size:
4978 3735 1849 771 680 391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 8565 8600 8565
Size: 35 12369
DraI TTT'AAA
Cuts at: 4415 6008 6938 9156 9403 4415
Size: 1593 930 2218 247 7416
Fragments arranged by size:
7416 2218 1593 930 247
DraIII CAC_nnn'GTG
Cuts at: 1737 5821 1737
Size: 4084 8320
DrdI GACnn_nn'nnGTC
Cuts at: 5593 10110 10968 5593
Size: 4517 858 7029
Fragments arranged by size:
7029 4517 858
EaeI y'GGCC_r
Cuts at: 167 224 236 1319 1341 2247 5170 5410 5573
Size: 57 12 1083 22 906 2923 240 163
Cuts at: 5573 5726 6369 7062 7700 7850 8235 8420 8573
Size: 153 643 693 638 150 385 185 153
Cuts at: 8573 9989 10163 10554 10581 11926 167
Size: 1416 174 391 27 1345 645
Fragments arranged by size:
2923 1416 1345 1083 906 693 645 643
638 391 385 240 185 174 163 153
153 150 57 27 22 12
EagI C'GGCC_G
Cuts at: 5170 5410 9989 5170
Size: 240 4579 7585
Fragments arranged by size:
7585 4579 240
EarI CTCTTCn'nnn_
Cuts at: 1910 2030 2434 2464 3460 3493 3953 4082 4639
Size: 120 404 30 996 33 460 129 557
Cuts at: 4639 5506 5672 5784 5964 9095 10427 10637 1910
Size: 867 166 112 180 3131 1332 210 3677
Fragments arranged by size:
3677 3131 1332 996 867 557 460 404
210 180 166 129 120 112 33 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 140 3253 5785 10612 10922 11068 12214 140
Size: 3113 2532 4827 310 146 1146 330
Fragments arranged by size:
4827 3113 2532 1146 330 310 146
EcoICRI GAG'CTC
Cuts at: 2427 2457 3639 4005 5393 5832 7568 9416 2427
Size: 30 1182 366 1388 439 1736 1848 5415
Fragments arranged by size:
5415 1848 1736 1388 1182 439 366 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 802 829 916 940 1129 1231 1405 1627
Size: 249 27 87 24 189 102 174 222
Cuts at: 1627 1780 2188 2322 2473 2575 2773 2836 2929
Size: 153 408 134 151 102 198 63 93
Cuts at: 2929 3319 3340 3600 3839 3868 4489 4784 4918
Size: 390 21 260 239 29 621 295 134
Cuts at: 4918 4930 5185 5253 5310 5350 5734 5896 6460
Size: 12 255 68 57 40 384 162 564
Cuts at: 6460 6802 7372 7476 7584 7618 8359 8632 8792
Size: 342 570 104 108 34 741 273 160
Cuts at: 8792 9226 10229 10661 11408 11676 553
Size: 434 1003 432 747 268 1281
Fragments arranged by size:
1281 1003 747 741 621 570 564 434
432 408 390 384 342 295 273 268
260 255 249 239 222 198 189 174
162 160 153 151 134 134 108 104
102 102 93 87 68 63 57 40
34 29 27 24 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3199 7546 8326 8797 9199 9202 3199
Size: 4347 780 471 402 3 6401
Fragments arranged by size:
6401 4347 780 471 402 3
EcoO109I rG'GnC_Cy
Cuts at: 231 361 915 927 1542 2229 2304 2745 3824
Size: 130 554 12 615 687 75 441 1079
Cuts at: 3824 4313 4593 5384 5401 5619 6066 7043 7044
Size: 489 280 791 17 218 447 977 1
Cuts at: 7044 7226 8265 8266 8433 9207 9543 231
Size: 182 1039 1 167 774 336 3092
Fragments arranged by size:
3092 1079 1039 977 791 774 687 615
554 489 447 441 336 280 218 182
167 130 75 17 12 1 1
EcoRI G'AATT_C
Cuts at: 8164 8977 9408 8164
Size: 813 431 11160
Fragments arranged by size:
11160 813 431
EcoRV GAT'ATC
Cuts at: 3894 3894
Size: 12404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 492 6741 7539 10423 492
Size: 6249 798 2884 2473
Fragments arranged by size:
6249 2884 2473 798
FseI GG_CCGG'CC
Cuts at: 7265 7265
Size: 12404
FspI TGC'GCA
Cuts at: 2473 5428 10185 2473
Size: 2955 4757 4692
Fragments arranged by size:
4757 4692 2955
HaeII r_GCGC'y
Cuts at: 2244 2589 5537 7097 7373 9838 10086 11108 12087
Size: 345 2948 1560 276 2465 248 1022 979
Cuts at: 12087 2244
Size: 2561
Fragments arranged by size:
2948 2561 2465 1560 1022 979 345 276
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1362 1394 1432 1464 1513 1545 1609 1641 2395
Size: 32 38 32 49 32 64 32 754
Cuts at: 2395 2427 2488 2500 2520 2532 3105 3137 3864
Size: 32 61 12 20 12 573 32 727
Cuts at: 3864 3896 4550 4582 5053 5085 5302 5334 5530
Size: 32 654 32 471 32 217 32 196
Cuts at: 5530 5562 5761 5793 7189 7221 7612 7644 8213
Size: 32 199 32 1396 32 391 32 569
Cuts at: 8213 8245 8516 8548 10246 10276 10278 10308 1362
Size: 32 271 32 1698 30 2 30 3458
Fragments arranged by size:
3458 1698 1396 754 727 654 573 569
471 391 271 217 199 196 64 61
49 38 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 2283 3138 5904 7994 9443 2283
Size: 855 2766 2090 1449 5244
Fragments arranged by size:
5244 2766 2090 1449 855
HindIII A'AGCT_T
Cuts at: 978 1505 8125 978
Size: 527 6620 5257
Fragments arranged by size:
6620 5257 527
KasI G'GCGC_C
Cuts at: 7093 7369 9834 10082 7093
Size: 276 2465 248 9415
Fragments arranged by size:
9415 2465 276 248
KpnI G_GTAC'C
Cuts at: 8823 9424 8823
Size: 601 11803
MfeI C'AATT_G
Cuts at: 5843 5843
Size: 12404
MluI A'CGCG_T
Cuts at: 11837 11837
Size: 12404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2851 3483 3887 4194 5163 5585 7167 9021 11075
Size: 632 404 307 969 422 1582 1854 2054
Cuts at: 11075 11259 2851
Size: 184 3996
Fragments arranged by size:
3996 2054 1854 1582 969 632 422 404
307 184
MscI TGG'CCA
Cuts at: 1321 1343 2249 5728 6371 7852 8575 10165 1321
Size: 22 906 3479 643 1481 723 1590 3560
Fragments arranged by size:
3560 3479 1590 1481 906 723 643 22
MslI CAynn'nnrTG
Cuts at: 568 793 1232 1315 1889 2125 2141 2143 3055
Size: 225 439 83 574 236 16 2 912
Cuts at: 3055 3575 3743 5284 7777 7798 7949 9335 10393
Size: 520 168 1541 2493 21 151 1386 1058
Cuts at: 10393 10520 568
Size: 127 2452
Fragments arranged by size:
2493 2452 1541 1386 1058 912 574 520
439 236 225 168 151 127 83 21
16 2
MspA1I CmG'CkG
Cuts at: 122 163 894 1131 1383 1647 1752 1758 1809
Size: 41 731 237 252 264 105 6 51
Cuts at: 1809 2007 2061 2397 2469 2832 3711 4293 5224
Size: 198 54 336 72 363 879 582 931
Cuts at: 5224 5465 5630 6432 6980 7004 7013 7031 7061
Size: 241 165 802 548 24 9 18 30
Cuts at: 7061 7085 7476 7638 7912 9515 9829 10189 11202
Size: 24 391 162 274 1603 314 360 1013
Cuts at: 11202 11447 122
Size: 245 1079
Fragments arranged by size:
1603 1079 1013 931 879 802 731 582
548 391 363 360 336 314 274 264
252 245 241 237 198 165 162 105
72 54 51 41 30 24 24 18
9 6
NaeI GCC'GGC
Cuts at: 204 5544 7117 7263 9264 10585 204
Size: 5340 1573 146 2001 1321 2023
Fragments arranged by size:
5340 2023 2001 1573 1321 146
NarI GG'CG_CC
Cuts at: 7094 7370 9835 10083 7094
Size: 276 2465 248 9415
Fragments arranged by size:
9415 2465 276 248
NcoI C'CATG_G
Cuts at: 1238 5723 1238
Size: 4485 7919
NgoMIV G'CCGG_C
Cuts at: 202 5542 7115 7261 9262 10583 202
Size: 5340 1573 146 2001 1321 2023
Fragments arranged by size:
5340 2023 2001 1573 1321 146
NheI G'CTAG_C
Cuts at: 7423 7423
Size: 12404
NotI GC'GGCC_GC
Cuts at: 5170 5170
Size: 12404
NruI TCG'CGA
Cuts at: 1375 1375
Size: 12404
NspI r_CATG'y
Cuts at: 498 798 1099 1446 2124 2974 3393 3742 4140
Size: 300 301 347 678 850 419 349 398
Cuts at: 4140 4309 4524 4750 6279 7872 9457 9957 10864
Size: 169 215 226 1529 1593 1585 500 907
Cuts at: 10864 498
Size: 2038
Fragments arranged by size:
2038 1593 1585 1529 907 850 678 500
419 398 349 347 301 300 226 215
169
PasI CC'CwG_GG
Cuts at: 3843 6588 3843
Size: 2745 9659
PciI A'CATG_T
Cuts at: 1442 2120 4746 6275 7868 10860 1442
Size: 678 2626 1529 1593 2992 2986
Fragments arranged by size:
2992 2986 2626 1593 1529 678
PflMI CCAn_nnn'nTGG
Cuts at: 1719 2061 5154 8964 1719
Size: 342 3093 3810 5159
Fragments arranged by size:
5159 3810 3093 342
PfoI T'CCnGG_A
Cuts at: 1718 2432 2741 3062 4040 4877 7123 8738 9083
Size: 714 309 321 978 837 2246 1615 345
Cuts at: 9083 9242 1718
Size: 159 4880
Fragments arranged by size:
4880 2246 1615 978 837 714 345 321
309 159
PmeI GTTT'AAAC
Cuts at: 9403 9403
Size: 12404
PmlI CAC'GTG
Cuts at: 3273 5484 7691 8192 8381 3273
Size: 2211 2207 501 189 7296
Fragments arranged by size:
7296 2211 2207 501 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 985 1017 4616 4648 7823 7855 8912 8944 8998
Size: 32 3599 32 3175 32 1057 32 54
Cuts at: 8998 9030 11543 11575 985
Size: 32 2513 32 1814
Fragments arranged by size:
3599 3175 2513 1814 1057 54 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 231 361 927 1542 2229 2304 2745 3824 4313
Size: 130 566 615 687 75 441 1079 489
Cuts at: 4313 4593 6066 8433 9207 231
Size: 280 1473 2367 774 3428
Fragments arranged by size:
3428 2367 1473 1079 774 687 615 566
489 441 280 130 75
PshAI GACnn'nnGTC
Cuts at: 3515 5192 5339 8729 3515
Size: 1677 147 3390 7190
Fragments arranged by size:
7190 3390 1677 147
PsiI TTA'TAA
Cuts at: 4706 4706
Size: 12404
PspOMI G'GGCC_C
Cuts at: 3249 5384 5400 7043 7225 8265 3249
Size: 2135 16 1643 182 1040 7388
Fragments arranged by size:
7388 2135 1643 1040 182 16
PspXI vC'TCGA_Gb
Cuts at: 2512 7179 2512
Size: 4667 7737
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4553 4585 4553
Size: 32 12372
PstI C_TGCA'G
Cuts at: 545 1022 1541 1808 2180 2273 2393 2468 2669
Size: 477 519 267 372 93 120 75 201
Cuts at: 2669 2846 3005 3181 6658 7176 7547 8642 8836
Size: 177 159 176 3477 518 371 1095 194
Cuts at: 8836 9203 9260 9377 9451 10136 545
Size: 367 57 117 74 685 2813
Fragments arranged by size:
3477 2813 1095 685 519 518 477 372
371 367 267 201 194 177 176 159
120 117 93 75 74 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12404
PvuII CAG'CTG
Cuts at: 894 1131 1383 1758 1809 2007 2061 2397 2469
Size: 237 252 375 51 198 54 336 72
Cuts at: 2469 2832 3711 4293 6432 7085 7912 9829 10189
Size: 363 879 582 2139 653 827 1917 360
Cuts at: 10189 894
Size: 3109
Fragments arranged by size:
3109 2139 1917 879 827 653 582 375
363 360 336 252 237 198 72 54
51
RsrII CG'GwC_CG
Cuts at: 10599 10599
Size: 12404
SacI G_AGCT'C
Cuts at: 2429 2459 3641 4007 5395 5834 7570 9418 2429
Size: 30 1182 366 1388 439 1736 1848 5415
Fragments arranged by size:
5415 1848 1736 1388 1182 439 366 30
SacII CC_GC'GG
Cuts at: 164 164
Size: 12404
SalI G'TCGA_C
Cuts at: 9441 9441
Size: 12404
SanDI GG'GwC_CC
Cuts at: 4593 4593
Size: 12404
SapI GCTCTTCn'nnn_
Cuts at: 2434 2464 5506 10427 10637 2434
Size: 30 3042 4921 210 4201
Fragments arranged by size:
4921 4201 3042 210 30
SbfI CC_TGCA'GG
Cuts at: 545 2273 2846 9203 9451 545
Size: 1728 573 6357 248 3498
Fragments arranged by size:
6357 3498 1728 573 248
ScaI AGT'ACT
Cuts at: 11921 11921
Size: 12404
SexAI A'CCwGG_T
Cuts at: 8612 8612
Size: 12404
SfcI C'TryA_G
Cuts at: 33 541 1018 1537 1804 2176 2269 2389 2464
Size: 508 477 519 267 372 93 120 75
Cuts at: 2464 2665 2842 3001 3031 3177 4570 6654 7172
Size: 201 177 159 30 146 1393 2084 518
Cuts at: 7172 7543 8301 8313 8493 8638 8832 9199 9256
Size: 371 758 12 180 145 194 367 57
Cuts at: 9256 9373 9447 10132 11125 11316 33
Size: 117 74 685 993 191 1121
Fragments arranged by size:
2084 1393 1121 993 758 685 519 518
508 477 372 371 367 267 201 194
191 180 177 159 146 145 120 117
93 75 74 57 30 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 7258 7258
Size: 12404
SfoI GGC'GCC
Cuts at: 7095 7371 9836 10084 7095
Size: 276 2465 248 9415
Fragments arranged by size:
9415 2465 276 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12404
SmaI CCC'GGG
Cuts at: 7224 7456 7616 9426 7224
Size: 232 160 1810 10202
Fragments arranged by size:
10202 1810 232 160
SmlI C'TyrA_G
Cuts at: 2512 3109 3127 3187 4819 6451 7179 7984 9559
Size: 597 18 60 1632 1632 728 805 1575
Cuts at: 9559 10474 10966 11228 2512
Size: 915 492 262 3688
Fragments arranged by size:
3688 1632 1632 1575 915 805 728 597
492 262 60 18
SnaBI TAC'GTA
Cuts at: 4058 9057 4058
Size: 4999 7405
SpeI A'CTAG_T
Cuts at: 67 6036 67
Size: 5969 6435
SphI G_CATG'C
Cuts at: 2974 3742 4524 9457 9957 2974
Size: 768 782 4933 500 5421
Fragments arranged by size:
5421 4933 782 768 500
StuI AGG'CCT
Cuts at: 671 1190 2689 5234 5776 671
Size: 519 1499 2545 542 7299
Fragments arranged by size:
7299 2545 1499 542 519
StyI C'CwwG_G
Cuts at: 227 1034 1238 1700 2649 4989 5723 6069 7409
Size: 807 204 462 949 2340 734 346 1340
Cuts at: 7409 7827 8436 9176 9538 9892 227
Size: 418 609 740 362 354 2739
Fragments arranged by size:
2739 2340 1340 949 807 740 734 609
462 418 362 354 346 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 2155 3646 4723 4770 5736 7741 8635 9987
Size: 1350 1491 1077 47 966 2005 894 1352
Cuts at: 9987 10120 10579 10788 805
Size: 133 459 209 2421
Fragments arranged by size:
2421 2005 1491 1352 1350 1077 966 894
459 209 133 47
TatI w'GTAC_w
Cuts at: 3206 3818 6278 11919 3206
Size: 612 2460 5641 3691
Fragments arranged by size:
5641 3691 2460 612
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1378 1410 1483 1515 2347 2379 2692 2724 6496
Size: 32 73 32 832 32 313 32 3772
Cuts at: 6496 6528 9172 9204 11921 11953 1378
Size: 32 2644 32 2717 32 1829
Fragments arranged by size:
3772 2717 2644 1829 832 313 73 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8614 10201 8614
Size: 1587 10817
XbaI T'CTAG_A
Cuts at: 9435 9435
Size: 12404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 596 927 1329 3555 5277 5979 7660 8568 8703
Size: 331 402 2226 1722 702 1681 908 135
Cuts at: 8703 9899 596
Size: 1196 3101
Fragments arranged by size:
3101 2226 1722 1681 1196 908 702 402
331 135
XhoI C'TCGA_G
Cuts at: 2512 3187 7179 2512
Size: 675 3992 7737
Fragments arranged by size:
7737 3992 675
XmaI C'CCGG_G
Cuts at: 7222 7454 7614 9424 7222
Size: 232 160 1810 10202
Fragments arranged by size:
10202 1810 232 160
XmnI GAAnn'nnTTC
Cuts at: 980 1871 2551 3988 4091 6445 6724 980
Size: 891 680 1437 103 2354 279 6660
Fragments arranged by size:
6660 2354 1437 891 680 279 103
ZraI GAC'GTC
Cuts at: 2640 3354 5039 2640
Size: 714 1685 10005
Fragments arranged by size:
10005 1685 714
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI ClaI FspAI HpaI NdeI
NsiI PacI SgrAI SrfI SspI SwaI