Restriction map for
pF1KE1599
(Circular) (Six-base) MAPSORT of: pF1KE1599.seq Check: 7792 from: 1 to: 6608
pF1KE1599 6608 bp
With 182 enzymes: SgfI *
August 26, 2010 17:36 ..
AccI GT'mk_AC
Cuts at: 992 999 3646 992
Size: 7 2647 3954
Fragments arranged by size:
3954 2647 7
Acc65I G'GTAC_C
Cuts at: 894 3624 894
Size: 2730 3878
AclI AA'CG_TT
Cuts at: 2788 6448 2788
Size: 3660 2948
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 3332 4433 4865 5612 5880 626
Size: 2706 1101 432 747 268 1354
Fragments arranged by size:
2706 1354 1101 747 432 268
AflIII A'CryG_T
Cuts at: 1687 2827 3960 5064 6041 1687
Size: 1140 1133 1104 977 2254
Fragments arranged by size:
2254 1140 1133 1104 977
AgeI A'CCGG_T
Cuts at: 4996 4996
Size: 6608
AhdI GACnn_n'nnGTC
Cuts at: 2831 2831
Size: 6608
AleI CACnn'nnGTG
Cuts at: 2618 2618
Size: 6608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1356 1356
Size: 6608
AlwNI CAG_nnn'CTG
Cuts at: 3913 5480 3913
Size: 1567 5041
ApaLI G'TGCA_C
Cuts at: 5378 5378
Size: 6608
ApoI r'AATT_y
Cuts at: 121 148 228 282 453 933 1124 1218 1232
Size: 27 80 54 171 480 191 94 14
Cuts at: 1232 2038 2206 2283 3156 3303 3612 5961 6217
Size: 806 168 77 873 147 309 2349 256
Cuts at: 6217 121
Size: 512
Fragments arranged by size:
2349 873 806 512 480 309 256 191
171 168 147 94 80 77 54 27
14
AseI AT'TA_AT
Cuts at: 20 2054 2582 2689 20
Size: 2034 528 107 3939
Fragments arranged by size:
3939 2034 528 107
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6608
AvaI C'yCGr_G
Cuts at: 1310 3628 1310
Size: 2318 4290
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3005 3038 3005
Size: 33 6575
BamHI G'GATC_C
Cuts at: 3633 3633
Size: 6608
BanI G'GyrC_C
Cuts at: 894 3624 4038 4286 4321 894
Size: 2730 414 248 35 3181
Fragments arranged by size:
3181 2730 414 248 35
BanII G_rGCy'C
Cuts at: 1562 3622 4652 1562
Size: 2060 1030 3518
Fragments arranged by size:
3518 2060 1030
BbeI G_GCGC'C
Cuts at: 4042 4290 4042
Size: 248 6360
BbsI GAAGACnn'nnnn_
Cuts at: 1004 3520 1004
Size: 2516 4092
BbvCI CC'TCA_GC
Cuts at: 3008 3008
Size: 6608
BciVI GTATCCnnnnn_n'
Cuts at: 2375 4506 5267 6608 2375
Size: 2131 761 1341 2375
Fragments arranged by size:
2375 2131 1341 761
BclI T'GATC_A
Cuts at: 1263 2502 3074 4128 1263
Size: 1239 572 1054 3743
Fragments arranged by size:
3743 1239 1054 572
BfrBI ATG'CAT
Cuts at: 1601 1601
Size: 6608
BglII A'GATC_T
Cuts at: 2946 4123 6160 2946
Size: 1177 2037 3394
Fragments arranged by size:
3394 2037 1177
BlpI GC'TnA_GC
Cuts at: 3720 3720
Size: 6608
Bme1580I G_kGCm'C
Cuts at: 2760 3415 4233 4326 5382 2760
Size: 655 818 93 1056 3986
Fragments arranged by size:
3986 1056 818 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 1897 1972 2387 3820 3901 4236 6380 1897
Size: 75 415 1433 81 335 2144 2125
Fragments arranged by size:
2144 2125 1433 415 335 81 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1097 4975 1097
Size: 3878 2730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1083 1092 3439 1083
Size: 9 2347 4252
Fragments arranged by size:
4252 2347 9
Bpu10I CC'TnA_GC
Cuts at: 3008 3008
Size: 6608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 3784 4663 5155 5453 2579
Size: 1205 879 492 298 3734
Fragments arranged by size:
3734 1205 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2931 6312 2931
Size: 3381 3227
BsaAI yAC'GTr
Cuts at: 3961 3961
Size: 6608
BsaBI GATnn'nnATC
Cuts at: 6 1648 3233 4122 6159 6
Size: 1642 1585 889 2037 455
Fragments arranged by size:
2037 1642 1585 889 455
BsaHI Gr'CG_yC
Cuts at: 4039 4287 4989 6485 4039
Size: 248 702 1496 4162
Fragments arranged by size:
4162 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3797 4318 4996 5270 5417 5828 3797
Size: 521 678 274 147 411 4577
Fragments arranged by size:
4577 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1070 1070
Size: 6608
BseYI C'CCAG_C
Cuts at: 3420 4033 4791 5368 5864 3420
Size: 613 758 577 496 4164
Fragments arranged by size:
4164 758 613 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 1940 2148 2350 434
Size: 1506 208 202 4692
Fragments arranged by size:
4692 1506 208 202
BsiEI CG_ry'CG
Cuts at: 104 4150 4196 5404 104
Size: 4046 46 1208 1308
Fragments arranged by size:
4046 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3622 4400 4590 5382 3622
Size: 778 190 792 4848
Fragments arranged by size:
4848 792 778 190
BsmI GAATG_Cn'
Cuts at: 1363 1603 1363
Size: 240 6368
Bsp1286I G_dGCh'C
Cuts at: 1562 2760 3415 3622 4233 4326 4400 4590 4652
Size: 1198 655 207 611 93 74 190 62
Cuts at: 4652 5382 1562
Size: 730 2788
Fragments arranged by size:
2788 1198 730 655 611 207 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5828 5828
Size: 6608
BspHI T'CATG_A
Cuts at: 678 5784 678
Size: 5106 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2617 3658 3872 4174 4555 2617
Size: 1041 214 302 381 4670
Fragments arranged by size:
4670 1041 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4900 4954 6438 6603 50
Size: 4850 54 1484 165 55
Fragments arranged by size:
4850 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1353 2175 4520 1353
Size: 822 2345 3441
Fragments arranged by size:
3441 2345 822
BsrFI r'CCGG_y
Cuts at: 4606 4787 4996 4606
Size: 181 209 6218
Fragments arranged by size:
6218 209 181
BssSI C'ACGA_G
Cuts at: 1082 4879 5237 1082
Size: 3797 358 2453
Fragments arranged by size:
3797 2453 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 3985 1927
Size: 2058 4550
BstBI TT'CG_AA
Cuts at: 286 4969 286
Size: 4683 1925
BstEII G'GTnAC_C
Cuts at: 3021 3351 3021
Size: 330 6278
BstXI CCAn_nnnn'nTGG
Cuts at: 807 1061 807
Size: 254 6354
BstYI r'GATC_y
Cuts at: 1 2946 3633 4100 4123 4458 4704 5716 5802
Size: 2945 687 467 23 335 246 1012 86
Cuts at: 5802 5814 6160 1
Size: 12 346 449
Fragments arranged by size:
2945 1012 687 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1000 1000
Size: 6608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1351 4558 4739 4902 1351
Size: 3207 181 163 3057
Fragments arranged by size:
3207 3057 181 163
BtsI GCAGTG_nn'
Cuts at: 660 678 1158 1340 1717 2136 3851 660
Size: 18 480 182 377 419 1715 3417
Fragments arranged by size:
3417 1715 480 419 377 182 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2903 2938 2903
Size: 35 6573
DraI TTT'AAA
Cuts at: 866 2354 3607 866
Size: 1488 1253 3867
Fragments arranged by size:
3867 1488 1253
DraIII CAC_nnn'GTG
Cuts at: 1640 2341 2395 3140 1640
Size: 701 54 745 5108
Fragments arranged by size:
5108 745 701 54
DrdI GACnn_nn'nnGTC
Cuts at: 967 3396 4314 5172 967
Size: 2429 918 858 2403
Fragments arranged by size:
2429 2403 918 858
EaeI y'GGCC_r
Cuts at: 2252 4193 4367 4758 4785 6130 2252
Size: 1941 174 391 27 1345 2730
Fragments arranged by size:
2730 1941 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4193 4193
Size: 6608
EarI CTCTTCn'nnn_
Cuts at: 2478 2667 2843 4631 4841 2478
Size: 189 176 1788 210 4245
Fragments arranged by size:
4245 1788 210 189 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4816 5126 5272 6418 4816
Size: 310 146 1146 5006
Fragments arranged by size:
5006 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3620 3620
Size: 6608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 1083 1092 3332 3439 4433 4865 5612 5880
Size: 457 9 2240 107 994 432 747 268
Cuts at: 5880 626
Size: 1354
Fragments arranged by size:
2240 1354 994 747 457 432 268 107
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2027
Size: 6608
EcoO109I rG'GnC_Cy
Cuts at: 2032 3747 2032
Size: 1715 4893
EcoRI G'AATT_C
Cuts at: 933 3612 933
Size: 2679 3929
EcoRV GAT'ATC
Cuts at: 2871 2871
Size: 6608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1809 4627 1809
Size: 2818 3790
FspI TGC'GCA
Cuts at: 4389 4389
Size: 6608
HaeII r_GCGC'y
Cuts at: 4042 4290 5312 6291 4042
Size: 248 1022 979 4359
Fragments arranged by size:
4359 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1522 1554 1867 1899 2139 2171 2817 2849 3163
Size: 32 313 32 240 32 646 32 314
Cuts at: 3163 3195 3316 3348 3451 3483 4450 4480 4482
Size: 32 121 32 103 32 967 30 2
Cuts at: 4482 4512 1522
Size: 30 3618
Fragments arranged by size:
3618 967 646 314 313 240 121 103
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1457 2994 3647 1457
Size: 1537 653 4418
Fragments arranged by size:
4418 1537 653
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 6608
KasI G'GCGC_C
Cuts at: 4038 4286 4038
Size: 248 6360
KpnI G_GTAC'C
Cuts at: 898 3628 898
Size: 2730 3878
MfeI C'AATT_G
Cuts at: 666 1495 2264 666
Size: 829 769 5010
Fragments arranged by size:
5010 829 769
MluI A'CGCG_T
Cuts at: 6041 6041
Size: 6608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5279 5463 5279
Size: 184 6424
MscI TGG'CCA
Cuts at: 2254 4369 2254
Size: 2115 4493
MslI CAynn'nnrTG
Cuts at: 805 1370 1686 2618 4597 4724 805
Size: 565 316 932 1979 127 2689
Fragments arranged by size:
2689 1979 932 565 316 127
MspA1I CmG'CkG
Cuts at: 307 3719 4033 4393 5406 5651 307
Size: 3412 314 360 1013 245 1264
Fragments arranged by size:
3412 1264 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4789 4789
Size: 6608
NarI GG'CG_CC
Cuts at: 4039 4287 4039
Size: 248 6360
NdeI CA'TA_TG
Cuts at: 839 839
Size: 6608
NgoMIV G'CCGG_C
Cuts at: 4787 4787
Size: 6608
NsiI A_TGCA'T
Cuts at: 1603 1603
Size: 6608
NspI r_CATG'y
Cuts at: 222 1691 2831 3534 3661 4161 5068 222
Size: 1469 1140 703 127 500 907 1762
Fragments arranged by size:
1762 1469 1140 907 703 500 127
PciI A'CATG_T
Cuts at: 1687 2827 5064 1687
Size: 1140 2237 3231
Fragments arranged by size:
3231 2237 1140
PfoI T'CCnGG_A
Cuts at: 2377 3455 2377
Size: 1078 5530
PmeI GTTT'AAAC
Cuts at: 3607 3607
Size: 6608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5747 5779 5747
Size: 32 6576
PpuMI rG'GwC_Cy
Cuts at: 2032 2032
Size: 6608
PsiI TTA'TAA
Cuts at: 572 3121 572
Size: 2549 4059
PspXI vC'TCGA_Gb
Cuts at: 1310 1310
Size: 6608
PstI C_TGCA'G
Cuts at: 3655 4340 3655
Size: 685 5923
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6608
PvuII CAG'CTG
Cuts at: 307 4033 4393 307
Size: 3726 360 2522
Fragments arranged by size:
3726 2522 360
RsrII CG'GwC_CG
Cuts at: 4803 4803
Size: 6608
SacI G_AGCT'C
Cuts at: 3622 3622
Size: 6608
SalI G'TCGA_C
Cuts at: 3645 3645
Size: 6608
SapI GCTCTTCn'nnn_
Cuts at: 4631 4841 4631
Size: 210 6398
SbfI CC_TGCA'GG
Cuts at: 3655 3655
Size: 6608
ScaI AGT'ACT
Cuts at: 1077 6125 1077
Size: 5048 1560
SfcI C'TryA_G
Cuts at: 33 3651 4336 5329 5520 33
Size: 3618 685 993 191 1121
Fragments arranged by size:
3618 1121 993 685 191
SfoI GGC'GCC
Cuts at: 4040 4288 4040
Size: 248 6360
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6608
SmaI CCC'GGG
Cuts at: 3630 3630
Size: 6608
SmlI C'TyrA_G
Cuts at: 1310 2594 3763 4678 5170 5432 1310
Size: 1284 1169 915 492 262 2486
Fragments arranged by size:
2486 1284 1169 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6608
SphI G_CATG'C
Cuts at: 222 3661 4161 222
Size: 3439 500 2669
Fragments arranged by size:
3439 2669 500
SspI AAT'ATT
Cuts at: 925 2549 925
Size: 1624 4984
StyI C'CwwG_G
Cuts at: 196 3742 4096 196
Size: 3546 354 2708
Fragments arranged by size:
3546 2708 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2406 4191 4324 4783 4992 2406
Size: 1785 133 459 209 4022
Fragments arranged by size:
4022 1785 459 209 133
TatI w'GTAC_w
Cuts at: 1075 1337 1462 1848 6123 1075
Size: 262 125 386 4275 1560
Fragments arranged by size:
4275 1560 386 262 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6125 6157 6125
Size: 32 6576
Tth111I GACn'n_nGTC
Cuts at: 4405 4405
Size: 6608
XbaI T'CTAG_A
Cuts at: 3639 3639
Size: 6608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 194 888 1825 4103 194
Size: 694 937 2278 2699
Fragments arranged by size:
2699 2278 937 694
XhoI C'TCGA_G
Cuts at: 1310 1310
Size: 6608
XmaI C'CCGG_G
Cuts at: 3628 3628
Size: 6608
XmnI GAAnn'nnTTC
Cuts at: 559 2472 2729 559
Size: 1913 257 4438
Fragments arranged by size:
4438 1913 257
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII Bsu36I BtgI ClaI FseI FspAI HindIII NcoI
NheI NotI NruI PacI PasI PflMI PmlI PshAI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI ZraI