Restriction map for
pF1KE1689
(Circular) (Six-base) MAPSORT of: pF1KE1689.seq Check: 9082 from: 1 to: 3710
pF1KE1689 3710 bp
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 439 439
Size: 3710
AccI GT'mk_AC
Cuts at: 748 748
Size: 3710
Acc65I G'GTAC_C
Cuts at: 726 726
Size: 3710
AclI AA'CG_TT
Cuts at: 3550 3550
Size: 3710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 712 1535 1967 2714 2982 484
Size: 228 823 432 747 268 1212
Fragments arranged by size:
1212 823 747 432 268 228
AflIII A'CryG_T
Cuts at: 1062 2166 3143 1062
Size: 1104 977 1629
Fragments arranged by size:
1629 1104 977
AgeI A'CCGG_T
Cuts at: 2098 2098
Size: 3710
AhdI GACnn_n'nnGTC
Cuts at: 411 411
Size: 3710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 293 293
Size: 3710
AlwNI CAG_nnn'CTG
Cuts at: 438 534 1015 2582 438
Size: 96 481 1567 1566
Fragments arranged by size:
1567 1566 481 96
ApaLI G'TGCA_C
Cuts at: 2480 2480
Size: 3710
ApoI r'AATT_y
Cuts at: 295 714 3063 3319 295
Size: 419 2349 256 686
Fragments arranged by size:
2349 686 419 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3710
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
AvaI C'yCGr_G
Cuts at: 194 212 478 730 194
Size: 18 266 252 3174
Fragments arranged by size:
3174 266 252 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 149 182 149
Size: 33 3677
BamHI G'GATC_C
Cuts at: 735 735
Size: 3710
BanI G'GyrC_C
Cuts at: 487 579 591 726 1140 1388 1423 487
Size: 92 12 135 414 248 35 2774
Fragments arranged by size:
2774 414 248 135 92 35 12
BanII G_rGCy'C
Cuts at: 369 479 724 1754 369
Size: 110 245 1030 2325
Fragments arranged by size:
2325 1030 245 110
BbeI G_GCGC'C
Cuts at: 583 595 1144 1392 583
Size: 12 549 248 2901
Fragments arranged by size:
2901 549 248 12
BbsI GAAGACnn'nnnn_
Cuts at: 472 700 472
Size: 228 3482
BbvCI CC'TCA_GC
Cuts at: 286 603 286
Size: 317 3393
BciVI GTATCCnnnnn_n'
Cuts at: 1608 2369 3710 1608
Size: 761 1341 1608
Fragments arranged by size:
1608 1341 761
BclI T'GATC_A
Cuts at: 1230 1230
Size: 3710
BglII A'GATC_T
Cuts at: 1225 3262 1225
Size: 2037 1673
BlpI GC'TnA_GC
Cuts at: 142 822 142
Size: 680 3030
Bme1580I G_kGCm'C
Cuts at: 360 490 1335 1428 2484 360
Size: 130 845 93 1056 1586
Fragments arranged by size:
1586 1056 845 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 370 675 922 1003 1338 3482 370
Size: 305 247 81 335 2144 598
Fragments arranged by size:
2144 598 335 305 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 3710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 3710
Bpu10I CC'TnA_GC
Cuts at: 286 603 286
Size: 317 3393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1765 2257 2555 886
Size: 879 492 298 2041
Fragments arranged by size:
2041 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3414 3414
Size: 3710
BsaAI yAC'GTr
Cuts at: 429 1063 429
Size: 634 3076
BsaBI GATnn'nnATC
Cuts at: 6 1224 3261 6
Size: 1218 2037 455
Fragments arranged by size:
2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 580 592 1141 1389 2091 3587 580
Size: 12 549 248 702 1496 703
Fragments arranged by size:
1496 703 702 549 248 12
BsaWI w'CCGG_w
Cuts at: 899 1420 2098 2372 2519 2930 899
Size: 521 678 274 147 411 1679
Fragments arranged by size:
1679 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 591 591
Size: 3710
BseYI C'CCAG_C
Cuts at: 300 483 557 1135 1893 2470 2966 300
Size: 183 74 578 758 577 496 1044
Fragments arranged by size:
1044 758 578 577 496 183 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 371 451 634 263
Size: 108 80 183 3339
Fragments arranged by size:
3339 183 108 80
BsiEI CG_ry'CG
Cuts at: 104 1252 1298 2506 104
Size: 1148 46 1208 1308
Fragments arranged by size:
1308 1208 1148 46
BsiHKAI G_wGCw'C
Cuts at: 239 369 724 1502 1692 2484 239
Size: 130 355 778 190 792 1465
Fragments arranged by size:
1465 792 778 355 190 130
Bsp1286I G_dGCh'C
Cuts at: 239 360 369 479 490 724 1335 1428 1502
Size: 121 9 110 11 234 611 93 74
Cuts at: 1502 1692 1754 2484 239
Size: 190 62 730 1465
Fragments arranged by size:
1465 730 611 234 190 121 110 93
74 62 11 9
BspEI T'CCGG_A
Cuts at: 2930 2930
Size: 3710
BspHI T'CATG_A
Cuts at: 2886 2886
Size: 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 428 439 760 974 1276 1657 428
Size: 11 321 214 302 381 2481
Fragments arranged by size:
2481 381 321 302 214 11
BsrBI CCG'CTC
Cuts at: 50 248 403 2002 2056 3540 3705 50
Size: 198 155 1599 54 1484 165 55
Fragments arranged by size:
1599 1484 198 165 155 55 54
BsrDI GCAATG_nn'
Cuts at: 1622 1622
Size: 3710
BsrFI r'CCGG_y
Cuts at: 509 521 576 1708 1889 2098 509
Size: 12 55 1132 181 209 2121
Fragments arranged by size:
2121 1132 209 181 55 12
BssSI C'ACGA_G
Cuts at: 1981 2339 1981
Size: 358 3352
BstAPI GCAn_nnn'nTGC
Cuts at: 438 1087 438
Size: 649 3061
BstBI TT'CG_AA
Cuts at: 2071 2071
Size: 3710
BstYI r'GATC_y
Cuts at: 1 735 1202 1225 1560 1806 2818 2904 2916
Size: 734 467 23 335 246 1012 86 12
Cuts at: 2916 3262 1
Size: 346 449
Fragments arranged by size:
1012 734 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1660 1841 2004 1660
Size: 181 163 3366
Fragments arranged by size:
3366 181 163
BtsI GCAGTG_nn'
Cuts at: 439 953 439
Size: 514 3196
DraI TTT'AAA
Cuts at: 709 709
Size: 3710
DrdI GACnn_nn'nnGTC
Cuts at: 1416 2274 1416
Size: 858 2852
EaeI y'GGCC_r
Cuts at: 151 341 1295 1469 1860 1887 3232 151
Size: 190 954 174 391 27 1345 629
Fragments arranged by size:
1345 954 629 391 190 174 27
EagI C'GGCC_G
Cuts at: 1295 1295
Size: 3710
EarI CTCTTCn'nnn_
Cuts at: 462 1733 1943 462
Size: 1271 210 2229
Fragments arranged by size:
2229 1271 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 274 1918 2228 2374 3520 274
Size: 1644 310 146 1146 464
Fragments arranged by size:
1644 1146 464 310 146
EcoICRI GAG'CTC
Cuts at: 367 722 367
Size: 355 3355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 493 712 1535 1967 2714 2982 484
Size: 9 219 823 432 747 268 1212
Fragments arranged by size:
1212 823 747 432 268 219 9
EcoO109I rG'GnC_Cy
Cuts at: 849 849
Size: 3710
EcoRI G'AATT_C
Cuts at: 714 714
Size: 3710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1729 1729
Size: 3710
FspI TGC'GCA
Cuts at: 1491 1491
Size: 3710
HaeII r_GCGC'y
Cuts at: 583 595 1144 1392 2414 3393 583
Size: 12 549 248 1022 979 900
Fragments arranged by size:
1022 979 900 549 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 234 266 397 429 1552 1582 1584 1614 234
Size: 32 131 32 1123 30 2 30 2330
Fragments arranged by size:
2330 1123 131 32 32 30 30 2
HincII GTy'rAC
Cuts at: 749 749
Size: 3710
KasI G'GCGC_C
Cuts at: 579 591 1140 1388 579
Size: 12 549 248 2901
Fragments arranged by size:
2901 549 248 12
KpnI G_GTAC'C
Cuts at: 730 730
Size: 3710
MluI A'CGCG_T
Cuts at: 3143 3143
Size: 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2565 2381
Size: 184 3526
MscI TGG'CCA
Cuts at: 153 343 1471 153
Size: 190 1128 2392
Fragments arranged by size:
2392 1128 190
MslI CAynn'nnrTG
Cuts at: 1699 1826 1699
Size: 127 3583
MspA1I CmG'CkG
Cuts at: 163 273 285 304 588 821 1135 1495 2508
Size: 110 12 19 284 233 314 360 1013
Cuts at: 2508 2753 163
Size: 245 1120
Fragments arranged by size:
1120 1013 360 314 284 245 233 110
19 12
NaeI GCC'GGC
Cuts at: 523 578 1891 523
Size: 55 1313 2342
Fragments arranged by size:
2342 1313 55
NarI GG'CG_CC
Cuts at: 580 592 1141 1389 580
Size: 12 549 248 2901
Fragments arranged by size:
2901 549 248 12
NgoMIV G'CCGG_C
Cuts at: 521 576 1889 521
Size: 55 1313 2342
Fragments arranged by size:
2342 1313 55
NspI r_CATG'y
Cuts at: 763 1263 2170 763
Size: 500 907 2303
Fragments arranged by size:
2303 907 500
PasI CC'CwG_GG
Cuts at: 172 172
Size: 3710
PciI A'CATG_T
Cuts at: 2166 2166
Size: 3710
PflMI CCAn_nnn'nTGG
Cuts at: 184 321 184
Size: 137 3573
PmeI GTTT'AAAC
Cuts at: 709 709
Size: 3710
PmlI CAC'GTG
Cuts at: 429 429
Size: 3710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 3678
PspXI vC'TCGA_Gb
Cuts at: 194 194
Size: 3710
PstI C_TGCA'G
Cuts at: 497 530 757 1442 497
Size: 33 227 685 2765
Fragments arranged by size:
2765 685 227 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3710
PvuII CAG'CTG
Cuts at: 163 273 285 304 1135 1495 163
Size: 110 12 19 831 360 2378
Fragments arranged by size:
2378 831 360 110 19 12
RsrII CG'GwC_CG
Cuts at: 1905 1905
Size: 3710
SacI G_AGCT'C
Cuts at: 369 724 369
Size: 355 3355
SalI G'TCGA_C
Cuts at: 747 747
Size: 3710
SapI GCTCTTCn'nnn_
Cuts at: 462 1733 1943 462
Size: 1271 210 2229
Fragments arranged by size:
2229 1271 210
SbfI CC_TGCA'GG
Cuts at: 497 757 497
Size: 260 3450
ScaI AGT'ACT
Cuts at: 3227 3227
Size: 3710
SfcI C'TryA_G
Cuts at: 33 370 493 526 753 1438 2431 2622 33
Size: 337 123 33 227 685 993 191 1121
Fragments arranged by size:
1121 993 685 337 227 191 123 33
SfoI GGC'GCC
Cuts at: 581 593 1142 1390 581
Size: 12 549 248 2901
Fragments arranged by size:
2901 549 248 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3710
SgrAI Cr'CCGG_yG
Cuts at: 576 576
Size: 3710
SmaI CCC'GGG
Cuts at: 732 732
Size: 3710
SmlI C'TyrA_G
Cuts at: 194 865 1780 2272 2534 194
Size: 671 915 492 262 1370
Fragments arranged by size:
1370 915 671 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3710
SphI G_CATG'C
Cuts at: 763 1263 763
Size: 500 3210
StyI C'CwwG_G
Cuts at: 844 1198 844
Size: 354 3356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1293 1426 1885 2094 1293
Size: 133 459 209 2909
Fragments arranged by size:
2909 459 209 133
TatI w'GTAC_w
Cuts at: 3225 3225
Size: 3710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 3678
Tth111I GACn'n_nGTC
Cuts at: 1507 1507
Size: 3710
XbaI T'CTAG_A
Cuts at: 741 741
Size: 3710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1205 1205
Size: 3710
XhoI C'TCGA_G
Cuts at: 194 194
Size: 3710
XmaI C'CCGG_G
Cuts at: 730 730
Size: 3710
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XmnI ZraI